• 1. College of Bioengineering, Chongqing University, Chongqing 400044, P.R.China;
  • 2. Shenzhen Lifotronic of Technology Co., Ltd., Shenzhen, Guangdong 518055, P.R.China;
GUO Xingming, Email: guoxm@cqu.edu.cn
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High performance liquid chromatography (HPLC) is currently the mainstream technology for detecting hemoglobin. Glycated hemoglobin (HbA1c) is a gold indicator for diagnosing diabetes, however, the accuracy of HbA1c test is affected by thalassemia factor hemoglobin F (HbF)/hemoglobin A2 (HbA2) and variant hemoglobin during HPLC analysis. In this study, a new anti-interference hemoglobin analysis system of HPLC is proposed. In this system, the high-pressure three-gradient elution method was improved, and the particle size and sieve plate aperture in the high-pressure chromatography column and the structure of the double-plunger reciprocating series high-pressure pump were optimized. The system could diagnose both HbA1c and thalassemia factor HbF/HbA2 and variant hemoglobin, and the performance of the system was anti-interference and stable. It is expected to achieve industrialization. In this study, the HbA1c and thalassemia factor HbF/HbA2 detection performance was compared between this system and the world’s first-line brand products such as Tosoh G8, Bio-Rad Ⅶ and D10 glycosylated hemoglobin analysis system. The results showed that the linear correlation between this system and the world-class system was good. The system is the first domestic hemoglobin analysis system by HPLC for screening of HbA1c and thalassemia factor HbF/HbA2 rapidly and accurately.

Citation: XU Yan, YAO Tiantian, HU Wenyong, ZHANG Bo, GUO Xingming. Anti-interference hemoglobin analysis system by high performance liquid chromatography. Journal of Biomedical Engineering, 2021, 38(5): 940-950. doi: 10.7507/1001-5515.202101069 Copy

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