Screening, domestication and identification of intestinal uric acid degrading bacteria in low uric acid population_Journal of Biomedical Engineering

Authors：

TIAN Tingting ¹ , CHEN Wujin ² , LIANG Meiting ¹ , Mayina Kahaer ³ , LI Rui ³ ,  SUN Yuping ^3,4

1. Basic Medical College, Xinjiang Medical University, Urumqi 830000, P. R. China;
2. Morphological Center, Basic Medical College, Xinjiang Medical University, Urumqi 830000, P. R. China;
3. Department of Microbiology, Basic Medical College, Xinjiang Medical University, Urumqi 830000, P. R. China;
4. Xinjiang Key Laboratory of Molecular Biology for Endemic Diseases, Xinjiang Medical University, Urumqi 830000, P. R. China;

Corresponding author：

SUN Yuping, Email: 544481723@qq.com

Keywords：

Low uric acid population; Uric acid degrading bacteria; Screen; Domestication; Uric acid degradation rate

DOI：

10.7507/1001-5515.202111028

Video：

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Abstract Full text Figures/Tables Video References Cited by

As the largest ecosystem of human body, intestinal microorganisms participate in the synthesis and metabolism of uric acid. Developing and utilizing intestinal bacteria to degrade uric acid might provide new ideas for the treatment of hyperuricemia. The fecal samples of people with low uric acid were inoculated into uric acid selective medium with the concentration of 1.5 mmol/L for preliminary screening, and the initially screened strains that may have degradation ability were domesticated by concentration gradient method, and the strains with high uric acid degradation rate were identified by 16S rRNA sequencing method. A strain of high-efficiency uric acid degrading bacteria was screened and domesticated from the feces of people with low uric acid. The degradation rate of uric acid could reach 50.2%. It was identified as Escherichia coli. The isolation and domestication of high efficient uric acid degrading strains can not only provide scientific basis for the study of the mechanism of intestinal microbial degradation of uric acid, but also reserve biological strains for the treatment of hyperuricemia and gout in the future.

Citation： TIAN Tingting, CHEN Wujin, LIANG Meiting, Mayina Kahaer, LI Rui, SUN Yuping. Screening, domestication and identification of intestinal uric acid degrading bacteria in low uric acid population. Journal of Biomedical Engineering, 2022, 39(4): 792-797. doi: 10.7507/1001-5515.202111028 Copy

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15.	Liu Hong, Zhang Xiaomin, Wang Yanli, et al. Prevalence of hyperuricemia among Chinese adults: a national cross-sectional survey using multistage, stratified sampling. J Nephrol, 2014, 27: 653-658.
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24.	王智. 抗高尿酸血症药物的研究进展. 中国城乡企业卫生, 2019, 34(5): 34-36.

1. 李丰琴, 叶志斌. 高尿酸血症与糖尿病及其并发症研究进展. 中国实用内科杂志, 2017, 37(6): 569-572.
2. Sandra S, Lesmana C R A, Purnamasari D, et al. Hyperuricemia as an independent risk factor for non-alcoholic fatty liver disease (NAFLD) progression evaluated using controlled attenuation parameter-transient elastography: lesson learnt from tertiary referral center. Diabetes Metab Syndr, 2019, 13(1): 424-428.
3. Su Pu, Hong Liu, Zhao Yifan, et al. Relationship between hyperuricemia and cardiovascular disease risk factors in a Chinese population: a cross-sectional study. Med Sci Monit, 2015, 12(21): 2707-2717.
4. 何向阳, 刘峥, 徐英, 等. 肥胖与四种常见慢性病的相关性分析. 预防医学, 2020, 32(7): 692-697.
5. 王天宇, 窦倩, 郭子坤, 等. 1947例体检人群高血压与高血糖和高尿酸血症及血脂异常关系分析. 社区医学杂志, 2020, 18(22): 1509-1511.
6. Lloyd-price J, Abu-ali G, Huttenhower C. The healthy human microbiome. Genome Med, 2016, 8(1): 51.
7. Guo Zhuang, Zhang Jiachao, Wang Zhanli, et al. Intestinal microbiota distinguish gout patients from healthy humans. Sci Rep, 2016, 6: 20602.
8. 纪泽敏. 基于临床病例筛选高尿酸血症相关的肠道菌群. 北京: 中央民族大学, 2020.
9. Ning Yaogui, Yang Guomei, Chen Yangchun, et al. Characteristics of the urinary microbiome from patients with gout: a prospective study. Front Endocrinol (Lausanne), 2020, 11: 272.
10. Yu Yiran, Liu Qiuping, Li Haichang, et al. Alterations of the gut microbiome associated with the treatment of hyperuricemia in male rats. Fro Mic, 2018, 9: 2233.
11. 任科雨, 勇春明, 金延春, 等. 青岛地区高尿酸血症患者的肠道菌群分析. 中国医师杂志, 2014, 16(12): 1649-1651.
12. 孙玉萍, 陈邬锦, 梁美婷,等. 用于筛选肠道降解尿酸菌株的高尿酸培养基及其制备方法: CN112831448A. 2021-05-25.
13. Ma Quantao, Li Yaqi, Li Pengfei, et al. Research progress in the relationship between type 2 diabetes mellitus and intestinal flora. Biomed Pharmacother, 2019, 117: 109138.
14. 董文学, 蒋雅琼, 马利锋, 等. 肠道菌群对尿酸代谢的影响. 胃肠病学和肝病学杂志, 2021, 30(1): 55-59.
15. Liu Hong, Zhang Xiaomin, Wang Yanli, et al. Prevalence of hyperuricemia among Chinese adults: a national cross-sectional survey using multistage, stratified sampling. J Nephrol, 2014, 27: 653-658.
16. Browne H P, Forster S C, Anonye B O, et al. Culturing of 'unculturable' human microbiota reveals novel taxa and extensive sporulation. Nature, 2016, 533(7604): 543-546.
17. Yu Yiran, Liu Qiuping, Li Haichang, et al. Alterations of the gut microbiome associated with the treatment of hyperuricaemia in male rats. Front Endocrinol, 2018, 9: 1-10.
18. Schultz A C, Nygaard P, Saxild H H. Functional analysis of 14 genes that constitute the purine catabolic pathway in Bacillus subtilis and evidence for a novel regulon controlled by the PucR transcription activator. J Bacteriol, 2001, 183(11): 3293-3302.
19. Lee Y, Lee D H, Kho C W, et al. Transthyretin-related proteins function to facilitate the hydrolysis of 5-hydroxyisourate, the end product of the uricase reaction. FEBS Lett, 2005, 579: 4769-4774.
20. Guzmán K, Badia J, Giménez R, et al. Transcriptional regulation of the gene cluster encoding allantoinase and guanine deaminase in Klebsiella pneumoniae. J Bacteriol, 2011, 193(9): 2197-2207.
21. Iwadate Y, Kato J I. Identification of a formate-dependent uric acid degradation pathway in Escherichia coli. J Bacteriol, 2019, 201(11): e00573-18.
22. 常宇骁. 人类肠道微生物培养组优化及肠道菌库构建与应用. 北京: 军事科学院, 2020.
23. Papakostas K, Frillingos S. Substrate selectivity of YgfU, a uric acid transporter from Escherichia coli. J Biol Chem, 2012, 287(19): 15684-15695.
24. 王智. 抗高尿酸血症药物的研究进展. 中国城乡企业卫生, 2019, 34(5): 34-36.

Journal of Biomedical Engineering

Screening, domestication and identification of intestinal uric acid degrading bacteria in low uric acid population

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