Mechanical and light-activated antibacterial properties of resin filled with Ag-TiO<sub>2 </sub>nanoparticles_Journal of Biomedical Engineering

Authors：

PAN Shiqi ¹ , LU Shuxin ¹ , LI Ruoyu ¹ ,  ZHANG Xiangyu ¹ , CHEN Weiyi ²

1. Shanxi Key Laboratory of Biomedical Metal Materials, College of Materials Science and Engineering, Taiyuan University of Technology, Taiyuan 030024, P. R. China;
2. College of Biomedical Engineering, Taiyuan University of Technology, Taiyuan 030024, P. R. China;

Corresponding author：

ZHANG Xiangyu, Email: zhangxiangyu@tyut.edu.cn

Keywords：

Composite resin; Mechanical property; Light-controlled antibacterial; Biocompatibility

DOI：

10.7507/1001-5515.202112067

Video：

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The poor mechanical property and vulnerability to bacterial infections are the main problems in clinic for dental restoration resins. Based on this problem, the purpose of this study is to synthesize silver-titanium dioxide (Ag-TiO₂) nanoparticles with good photocatalytic properties, and add them to the composite resin to improve the mechanical properties and photocatalytic antibacterial capability of the resin. The microstructure and chemical composition of Ag-TiO₂ nanoparticles and composite resins were characterized. The results indicated that Ag existed in both metallic and silver oxide state in the Ag-TiO₂, and Ag-TiO₂ nanoparticles were uniformly dispersed in the resins. The results of mechanical experiments suggested that the mechanical properties of the composite resin were significantly improved due to the incorporation of Ag-TiO₂ nanoparticles. The antibacterial results indicated that the Ag-TiO₂ nanoparticle-filled composite resins exhibited excellent antibacterial activities under 660 nm light irradiation for 10 min due to the photocatalysis, and the Ag-TiO₂ nanoparticle-filled composite resins could also exhibit excellent antibacterial activities after contact with bacteria for 24 h without light irradiation because of the release of Ag ions. In summary, this study provides a new antibacterial idea for the field of dental composite resins.

Citation： PAN Shiqi, LU Shuxin, LI Ruoyu, ZHANG Xiangyu, CHEN Weiyi. Mechanical and light-activated antibacterial properties of resin filled with Ag-TiO₂nanoparticles. Journal of Biomedical Engineering, 2022, 39(4): 749-758. doi: 10.7507/1001-5515.202112067 Copy

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11.	Shi Y Y, Sun B, Zhou Z, et al. Size-controlled and large-scale synthesis of organic-soluble Ag nanocrystals in water and their formation mechanism. Progress in Natural Science, 2011, 21(6): 447-454.
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13.	Wang J, Zhang C, Yang Y, et al. Poly (vinyl alcohol)(PVA) hydrogel incorporated with Ag/TiO2 for rapid sterilization by photoinspired radical oxygen species and promotion of wound healing. Appl Surf Sci, 2019, 494: 708-720.
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15.	Sun J, Petersen E J, Watson S S, et al. Biophysical characterization of functionalized Titania nanoparticles and their application in dental adhesives. Acta Biomater, 2017, 53: 585-597.
16.	Dias H B, Bernardi M I B, Marangoni V S, et al. Synthesis, characterization and application of Ag doped ZnO nanoparticles in a composite resin. Mater Sci Eng C Mater Biol Appl, 2019, 96: 391-401.
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24.	Bai X, Lin C, Wang Y, et al. Preparation of Zn doped mesoporous silica nanoparticles (Zn-MSNs) for the improvement of mechanical and antibacterial properties of dental resin composites. Dent Mater, 2020, 36(6): 794-807.
25.	Feng Z, Liu X, Tan L, et al. Electrophoretic deposited stable chitosan@MoS(2) coating with rapid in situ bacteria-killing ability under dual-light irradiation. Small, 2018, 14(21): e1704347.
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27.	Zhang X, Zhang G, Zhang H, et al. A bifunctional hydrogel incorporated with CuS@MoS2 microspheres for disinfection and improved wound healing. Chem Engineer J, 2020, 382: 122849.
28.	Zhang Guannan, Yang Yongqiang, Shi Jing, et al. Near-infrared light II-assisted rapid biofilm elimination platform for bone implants at mild temperature. Biomaterials, 2021, 269: 120634.
29.	Mao C, Xiang Y, Liu X, et al. Photo-inspired antibacterial activity and wound healing acceleration by hydrogel embedded with Ag/Ag@AgCl/ZnO nanostructures. ACS Nano, 2017, 11(9): 9010-9021.
30.	Tan L, Li J, Liu X, et al. In situ disinfection through photoinspired radical oxygen species storage and thermal-triggered release from black phosphorous with strengthened chemical stability. Small, 2018: 1703197.
31.	Zhang X, Li M, He X, et al. Antibacterial activity of single crystalline silver-doped anatase TiO2 nanowire arrays. Appl Surf Sci, 2016, 372: 139-144.
32.	Zhang L, Guo J, Huang X, et al. The dual function of Cu-doped TiO(2) coatings on titanium for application in percutaneous implants. J Mater Chem B, 2016, 4(21): 3788-3800.
33.	Gao A, Hang R, Huang X, et al. The effects of titania nanotubes with embedded silver oxide nanoparticles on bacteria and osteoblasts. Biomaterials, 2014, 35(13): 4223-4235.
34.	Cheng H, Wang J, Yang Y, et al. Ti3C2TX MXene modified with ZnTCPP with bacteria capturing capability and enhanced visible light photocatalytic antibacterial activity. Small, 2022: 2200857.

1. Khalaf M E, Alomari Q D, Omar R. Factors relating to usage patterns of amalgam and resin composite for posterior restorations-a prospective analysis. J Dent, 2014, 42(7): 785-792.
2. 毛驭川, 张璐斯, 陈红艳, 等. 抗菌剂用于牙科复合树脂的研究进展. 材料科学与工艺, 2021, 29(2): 1-19.
3. Nedeljkovic I, de Munck J, Vanloy A, et al. Secondary caries: prevalence, characteristics, and approach. Clin Oral Investig, 2020, 24(2): 683-691.
4. Boaro L C, Campos L M, Varca G C, et al. Antibacterial resin-based composite containing chlorhexidine for dental applications. Dent Mater, 2019, 35(6): 909-918.
5. Beyth N, Farah S, Domb A J, et al. Antibacterial dental resin composites. React Funct Polym, 2014, 75: 81-88.
6. 周泽瑛, 张静月, 牛菊, 等. 牙科树脂材料抗菌性能的研究进展. 口腔疾病防治, 2021, 29(9): 638-643.
7. Ilie N, Hilton T J, Heintze S D, et al. Academy of dental materials guidance-resin composites: part I-mechanical properties. Dent Mater, 2017, 33(8): 880-894.
8. Sun J, Watson S S, Allsopp D, et al. Tuning photo-catalytic activities of TiO₂ nanoparticles using dimethacrylate resins. Dent Mater, 2016, 32(3): 363-372.
9. Raorane D V, Chaughule R S, Pednekar S R, et al. Experimental synthesis of size-controlled TiO₂ nanofillers and their possible use as composites in restorative dentistry. Saudi Dent J, 2019, 31(2): 194-203.
10. Dias H B, Bernardi M B, Bauab T M, et al. Titanium dioxide and modified titanium dioxide by silver nanoparticles as an antibiofilm filler content for composite resins. Dent Mater, 2019, 35(2): 36-46.
11. Shi Y Y, Sun B, Zhou Z, et al. Size-controlled and large-scale synthesis of organic-soluble Ag nanocrystals in water and their formation mechanism. Progress in Natural Science, 2011, 21(6): 447-454.
12. 董伟, 廖丽斐. 纳米银系抗菌剂在口腔粘接材料中的应用现状及展望. 粘接, 2021, 46(6): 68-71.
13. Wang J, Zhang C, Yang Y, et al. Poly (vinyl alcohol)(PVA) hydrogel incorporated with Ag/TiO2 for rapid sterilization by photoinspired radical oxygen species and promotion of wound healing. Appl Surf Sci, 2019, 494: 708-720.
14. Esteban F L, Hiers R D, Larson P, et al. Antibacterial dental adhesive resins containing nitrogen-doped titanium dioxide nanoparticles. Mater Sci Eng C Mater Biol Appl, 2018, 93: 931-943.
15. Sun J, Petersen E J, Watson S S, et al. Biophysical characterization of functionalized Titania nanoparticles and their application in dental adhesives. Acta Biomater, 2017, 53: 585-597.
16. Dias H B, Bernardi M I B, Marangoni V S, et al. Synthesis, characterization and application of Ag doped ZnO nanoparticles in a composite resin. Mater Sci Eng C Mater Biol Appl, 2019, 96: 391-401.
17. Chambers C, Stewart S B, Su B, et al. Silver doped titanium dioxide nanoparticles as antimicrobial additives to dental polymers. Dent Mater, 2017, 33(3): e115-e123.
18. 国家食品药品监督管理局. YY 1042-2011牙科学聚合物基修复材料. 北京: 质检出版社, 2011.
19. Yang G, Yin H, Liu W, et al. Synergistic Ag/TiO2-N photocatalytic system and its enhanced antibacterial activity towards acinetobacter baumannii. Appl Catal B-Environ, 2018, 224: 175-182.
20. Saravanan R, Manoj D, Qin J, et al. Mechanothermal synthesis of Ag/TiO2 for photocatalytic methyl orange degradation and hydrogen production. Process Saf Environ, 2018, 120: 339-347.
21. Gumy D, Morais C, Bowen P, et al. Catalytic activity of commercial of TiO₂ powders for the abatement of the bacteria (E. coli) under solar simulated light: influence of the isoelectric point. Applied Catalysis B-Environmental, 2006, 63(1-2): 76-84.
22. Liao H, Andersson A S, Sutherland D, et al. Response of rat osteoblast-like cells to microstructured model surfaces in vitro. Biomaterials, 2003, 24(4): 649-654.
23. Samuel S P, Li S, Mukherjee I, et al. Mechanical properties of experimental dental composites containing a combination of mesoporous and nonporous spherical silica as fillers. Dent Mater, 2009, 25(3): 296-301.
24. Bai X, Lin C, Wang Y, et al. Preparation of Zn doped mesoporous silica nanoparticles (Zn-MSNs) for the improvement of mechanical and antibacterial properties of dental resin composites. Dent Mater, 2020, 36(6): 794-807.
25. Feng Z, Liu X, Tan L, et al. Electrophoretic deposited stable chitosan@MoS(2) coating with rapid in situ bacteria-killing ability under dual-light irradiation. Small, 2018, 14(21): e1704347.
26. Deepagan V G, You D G, Um W, et al. Long-circulating Au-TiO₂ nanocomposite as a sonosensitizer for ROS-mediated eradication of cancer. Nano Lett, 2016, 16(10): 6257-6264.
27. Zhang X, Zhang G, Zhang H, et al. A bifunctional hydrogel incorporated with CuS@MoS2 microspheres for disinfection and improved wound healing. Chem Engineer J, 2020, 382: 122849.
28. Zhang Guannan, Yang Yongqiang, Shi Jing, et al. Near-infrared light II-assisted rapid biofilm elimination platform for bone implants at mild temperature. Biomaterials, 2021, 269: 120634.
29. Mao C, Xiang Y, Liu X, et al. Photo-inspired antibacterial activity and wound healing acceleration by hydrogel embedded with Ag/Ag@AgCl/ZnO nanostructures. ACS Nano, 2017, 11(9): 9010-9021.
30. Tan L, Li J, Liu X, et al. In situ disinfection through photoinspired radical oxygen species storage and thermal-triggered release from black phosphorous with strengthened chemical stability. Small, 2018: 1703197.
31. Zhang X, Li M, He X, et al. Antibacterial activity of single crystalline silver-doped anatase TiO2 nanowire arrays. Appl Surf Sci, 2016, 372: 139-144.
32. Zhang L, Guo J, Huang X, et al. The dual function of Cu-doped TiO(2) coatings on titanium for application in percutaneous implants. J Mater Chem B, 2016, 4(21): 3788-3800.
33. Gao A, Hang R, Huang X, et al. The effects of titania nanotubes with embedded silver oxide nanoparticles on bacteria and osteoblasts. Biomaterials, 2014, 35(13): 4223-4235.
34. Cheng H, Wang J, Yang Y, et al. Ti3C2TX MXene modified with ZnTCPP with bacteria capturing capability and enhanced visible light photocatalytic antibacterial activity. Small, 2022: 2200857.

Journal of Biomedical Engineering

Mechanical and light-activated antibacterial properties of resin filled with Ag-TiO₂nanoparticles

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Journal of Biomedical Engineering

Mechanical and light-activated antibacterial properties of resin filled with Ag-TiO2 nanoparticles

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Mechanical and light-activated antibacterial properties of resin filled with Ag-TiO₂nanoparticles