Frontiers and development in live-cell super-resolution fluorescence microscopy_Journal of Biomedical Engineering

Authors：

CHENG Yufei ¹ , LI Wei ² , JIN Tingting ³ , WU Sisi ¹ ,  ZHANG Longhao ²

1. Core Facilities, West China Hospital of Sichuan University, Chengdu 610041, P. R. China;
2. Department of Medical Discipline Construction, West China hospital of Sichuan University, Chengdu 610041, P. R. China;
3. Department of Health Policy and Management, West China School of Public Health and West China Fourth Hospital, Sichuan University, Chengdu 610041, P. R. China;

Corresponding author：

Keywords：

Live-cell; Super-resolution fluorescence microscopy; Structured illumination microscopy; Stimulated emission depletion microscopy; Minimal photon fluxes

DOI：

10.7507/1001-5515.202210060

Video：

Export PDF Favorites Scan Get Citation

Abstract Full text Figures/Tables Video References Cited by

This paper reviews the research progress on live-cell super-resolution fluorescence microscopy, discusses the current research status and hotspots in this field, and summarizes the technological application of super-resolution fluorescence microscopy for live-cell imaging. To date, this field has gained progress in numerous aspects. Specifically, the structured illumination microscopy, stimulated emission depletion microscopy, and the recently introduced minimal photon fluxes microscopy are the current research hotspots. According to the current progress in this field, future development trend is likely to be largely driven by artificial intelligence as well as advances in fluorescent probes and relevant labelling methods.

Citation： CHENG Yufei, LI Wei, JIN Tingting, WU Sisi, ZHANG Longhao. Frontiers and development in live-cell super-resolution fluorescence microscopy. Journal of Biomedical Engineering, 2023, 40(1): 180-184. doi: 10.7507/1001-5515.202210060 Copy

1.	Tønnesen J, Inavalli V V G K, Nägerl U V. Super-resolution imaging of the extracellular space in living brain tissue. Cell, 2018, 172(5): 1108-1121.
2.	Shim S H, Xia C L, Zhong G S, et al. Super-resolution fluorescence imaging of organelles in live cells with photoswitchable membrane probes. Proc Natl Acad Sci U S A, 2012, 109(35): 13978-13983.
3.	Nixon-Abell J, Obara C J, Weigel A V, et al. Increased spatiotemporal resolution reveals highly dynamic dense tubular matrices in the peripheral ER. Science. 2016, 354(6311): aaf3928.
4.	Gustafsson M G. Surpassing the lateral resolution limit by a factor of two using structured illumination microscopy. J Microsc, 2000, 198(Pt 2): 82-87.
5.	陈廷爱,陈龙超,李慧,等. 结构光照明超分辨光学显微成像技术与展望. 中国光学, 2018, 11(3): 307-328.
6.	张娇,何勤,武泽凯,等. 超分辨显微成像技术在活细胞成像中的应用与发展. 生物化学与生物物理进展, 2021, 48(11): 1301-1315.
7.	Ling C, Zhang C L, Wang M Q, et al. Fast structured illumination microscopy via deep learning. Photonics Research, 2020, 8(8): 1350-1359.
8.	Jin L H, Liu B, Zhao F Q, et al. Deep learning enables structured illumination microscopy with low light levels and enhanced speed. Nat Commun, 2020, 11(1): 1934.
9.	Förster R, Wicker K, Müller W, et al. Motion artefact detection in structured illumination microscopy or live cell imaging. Opt Express, 2016, 24(19): 22121-22124.
10.	Foster R, Muller W, Richter R, et al. Automated distinction of shearing and distortion artefacts in structured illumination microscopy. Opt Express, 2018, 26(16): 20680-20694.
11.	Wen G, Li S M, Wang L B, et al. High-fidelity structured illumination microscopy by point-spread-function engineering. Light Sci Appl, 2021, 10(1): 70.
12.	Huang X X, Fan J C, Li L J, et al. Fast, long-term, super-resolution imaging with Hessian structured illumination microscopy. Nat Biotechnol, 2018, 36(5): 451-459.
13.	Zhao W S, Zhao S Q, Li L J, et al. Sparse deconvolution improves the resolution of live-cell super-resolution fluorescence microscopy. Nat Biotechnol, 2022, 40(4): 606-617.
14.	Hell S W, Wichmann J. Breaking the diffraction resolution limit by stimulated emission: stimulated-emission-depletion fluorescence microscopy. Optics Letters, 1994, 19(11): 780-782.
15.	Schermelleh L, Ferrand A, Huser T, et al. Super-resolution microscopy demystified. Nat Cell Biol, 2019, 21(1): 72-84.
16.	Takasaki K T, Ding J B, Sabatini B L. Live-cell superresolution imaging by pulsed STED two-photon excitation microscopy. Biophys J, 2013, 104(4): 770-777.
17.	Vicidomini G, Hernandez I C, d'Amora M, et al. Gated CW-STED microscopy: a versatile tool for biological nanometer scale investigation. Methods, 2014, 66(2): 124-130.
18.	Hernández I C, Buttafava M, Boso G, et al. Gated STED microscopy with time-gated single-photon avalanche diode. Biomed Opt Express, 2015, 6(6): 2258-2267.
19.	Castello M, Tortarolo G, Hernández I C, et al. Gated-sted microscopy with subnanosecond pulsed fiber laser for reducing photobleaching. Microsc Res Tech, 2016, 79(9): 785-791.
20.	Liu S C, Zhang Z M, Han Y B, et al. Ratiometric photon reassignment based on fluorescence lifetime to improve resolution in pulse STED microscopy. Opt Lett, 2021, 46(13): 3304-3307.
21.	Heine J, Reuss M, Harke B, et al. Adaptive-illumination STED nanoscopy. Proc Natl Acad Sci U S A, 2017, 144(37): 9797-9802.
22.	Schloetel J G, Heine J, Cowman A F, et al. Guided STED nanoscopy enables super-resolution imaging of blood stage malaria parasites. Sci Rep, 2019, 9(1): 4674.
23.	D’Este E, Kamin D, Gottfert F, et al. STED nanoscopy reveals the ubiquity of subcortical cytoskeleton periodicity in living neurons. Cell Rep, 2015, 10(8): 1246-1251.
24.	Lukinavicius G, Mitronova G Y, Schnorrenberg S, et al. Fluorescent dyes and probes for super-resolution microscopy of microtubules and tracheoles in living cells and tissues. Chem Sci, 2018, 9(13): 3324-3334.
25.	Wang C G, Taki M, Sato Y, et al. A photostable fluorescent marker for the superresolution live imaging of the dynamic structure of the mitochondrial criste. Proc Natl Acad Sci U S A, 2019, 116(32): 15817-15822.
26.	Yang X S, Yang Z G, Wu Z Y, et al. Mitochondrial dynamics quantitatively revealed by STED nanoscopy with an enhanced squaraine variant probe. Nat Commun, 2020, 11(1): 3699.
27.	Balzarotti F, Eilers Y, Gwosch K C, et al. Nanometer resolution imaging and tracking of fluorescent molecules with minimal photon fluxes. Science, 2017, 355(6325): 606-612.
28.	Eilers Y, Ta H, Gwosch K C, et al. MINFLUX monitors rapid molecular jumps with superior spatiotemporal resolution. Proc Natl Acad Sci U S A, 2018, 115(24): 6117-6122.
29.	Gwosch K C, Pape J K, Balzarotti F, et al. MINFLUX nanoscopy delivers 3D multicolor nanometer resolution in cells. Nat Methods, 2020, 17(2): 217-224.
30.	Pape J K, Stephan T, Balzarotti F, et al. Multicolor 3D MINFLUX nanoscopy of mitochondrial MICOS proteins. Proc Natl Acad Sci U S A, 2020, 117(34): 20607-20614.
31.	Liu S, Hoess P, Ries J. Super-resolution microscopy for structural cell biology. Annu Rev Biophys, 2022, 51: 301-326.
32.	Schmidt R, Weihs T, Wurm C A, et al. MINFLUX nanometer-scale 3D imaging and microsecond-range tracking on a common fluorescence microscope. Nat Commun, 2021, 12(1): 1478.

1. Tønnesen J, Inavalli V V G K, Nägerl U V. Super-resolution imaging of the extracellular space in living brain tissue. Cell, 2018, 172(5): 1108-1121.
2. Shim S H, Xia C L, Zhong G S, et al. Super-resolution fluorescence imaging of organelles in live cells with photoswitchable membrane probes. Proc Natl Acad Sci U S A, 2012, 109(35): 13978-13983.
3. Nixon-Abell J, Obara C J, Weigel A V, et al. Increased spatiotemporal resolution reveals highly dynamic dense tubular matrices in the peripheral ER. Science. 2016, 354(6311): aaf3928.
4. Gustafsson M G. Surpassing the lateral resolution limit by a factor of two using structured illumination microscopy. J Microsc, 2000, 198(Pt 2): 82-87.
5. 陈廷爱,陈龙超,李慧,等. 结构光照明超分辨光学显微成像技术与展望. 中国光学, 2018, 11(3): 307-328.
6. 张娇,何勤,武泽凯,等. 超分辨显微成像技术在活细胞成像中的应用与发展. 生物化学与生物物理进展, 2021, 48(11): 1301-1315.
7. Ling C, Zhang C L, Wang M Q, et al. Fast structured illumination microscopy via deep learning. Photonics Research, 2020, 8(8): 1350-1359.
8. Jin L H, Liu B, Zhao F Q, et al. Deep learning enables structured illumination microscopy with low light levels and enhanced speed. Nat Commun, 2020, 11(1): 1934.
9. Förster R, Wicker K, Müller W, et al. Motion artefact detection in structured illumination microscopy or live cell imaging. Opt Express, 2016, 24(19): 22121-22124.
10. Foster R, Muller W, Richter R, et al. Automated distinction of shearing and distortion artefacts in structured illumination microscopy. Opt Express, 2018, 26(16): 20680-20694.
11. Wen G, Li S M, Wang L B, et al. High-fidelity structured illumination microscopy by point-spread-function engineering. Light Sci Appl, 2021, 10(1): 70.
12. Huang X X, Fan J C, Li L J, et al. Fast, long-term, super-resolution imaging with Hessian structured illumination microscopy. Nat Biotechnol, 2018, 36(5): 451-459.
13. Zhao W S, Zhao S Q, Li L J, et al. Sparse deconvolution improves the resolution of live-cell super-resolution fluorescence microscopy. Nat Biotechnol, 2022, 40(4): 606-617.
14. Hell S W, Wichmann J. Breaking the diffraction resolution limit by stimulated emission: stimulated-emission-depletion fluorescence microscopy. Optics Letters, 1994, 19(11): 780-782.
15. Schermelleh L, Ferrand A, Huser T, et al. Super-resolution microscopy demystified. Nat Cell Biol, 2019, 21(1): 72-84.
16. Takasaki K T, Ding J B, Sabatini B L. Live-cell superresolution imaging by pulsed STED two-photon excitation microscopy. Biophys J, 2013, 104(4): 770-777.
17. Vicidomini G, Hernandez I C, d'Amora M, et al. Gated CW-STED microscopy: a versatile tool for biological nanometer scale investigation. Methods, 2014, 66(2): 124-130.
18. Hernández I C, Buttafava M, Boso G, et al. Gated STED microscopy with time-gated single-photon avalanche diode. Biomed Opt Express, 2015, 6(6): 2258-2267.
19. Castello M, Tortarolo G, Hernández I C, et al. Gated-sted microscopy with subnanosecond pulsed fiber laser for reducing photobleaching. Microsc Res Tech, 2016, 79(9): 785-791.
20. Liu S C, Zhang Z M, Han Y B, et al. Ratiometric photon reassignment based on fluorescence lifetime to improve resolution in pulse STED microscopy. Opt Lett, 2021, 46(13): 3304-3307.
21. Heine J, Reuss M, Harke B, et al. Adaptive-illumination STED nanoscopy. Proc Natl Acad Sci U S A, 2017, 144(37): 9797-9802.
22. Schloetel J G, Heine J, Cowman A F, et al. Guided STED nanoscopy enables super-resolution imaging of blood stage malaria parasites. Sci Rep, 2019, 9(1): 4674.
23. D’Este E, Kamin D, Gottfert F, et al. STED nanoscopy reveals the ubiquity of subcortical cytoskeleton periodicity in living neurons. Cell Rep, 2015, 10(8): 1246-1251.
24. Lukinavicius G, Mitronova G Y, Schnorrenberg S, et al. Fluorescent dyes and probes for super-resolution microscopy of microtubules and tracheoles in living cells and tissues. Chem Sci, 2018, 9(13): 3324-3334.
25. Wang C G, Taki M, Sato Y, et al. A photostable fluorescent marker for the superresolution live imaging of the dynamic structure of the mitochondrial criste. Proc Natl Acad Sci U S A, 2019, 116(32): 15817-15822.
26. Yang X S, Yang Z G, Wu Z Y, et al. Mitochondrial dynamics quantitatively revealed by STED nanoscopy with an enhanced squaraine variant probe. Nat Commun, 2020, 11(1): 3699.
27. Balzarotti F, Eilers Y, Gwosch K C, et al. Nanometer resolution imaging and tracking of fluorescent molecules with minimal photon fluxes. Science, 2017, 355(6325): 606-612.
28. Eilers Y, Ta H, Gwosch K C, et al. MINFLUX monitors rapid molecular jumps with superior spatiotemporal resolution. Proc Natl Acad Sci U S A, 2018, 115(24): 6117-6122.
29. Gwosch K C, Pape J K, Balzarotti F, et al. MINFLUX nanoscopy delivers 3D multicolor nanometer resolution in cells. Nat Methods, 2020, 17(2): 217-224.
30. Pape J K, Stephan T, Balzarotti F, et al. Multicolor 3D MINFLUX nanoscopy of mitochondrial MICOS proteins. Proc Natl Acad Sci U S A, 2020, 117(34): 20607-20614.
31. Liu S, Hoess P, Ries J. Super-resolution microscopy for structural cell biology. Annu Rev Biophys, 2022, 51: 301-326.
32. Schmidt R, Weihs T, Wurm C A, et al. MINFLUX nanometer-scale 3D imaging and microsecond-range tracking on a common fluorescence microscope. Nat Commun, 2021, 12(1): 1478.

Previous Article
Research progress on image-based calculation of coronary artery fractional flow reserve
Next Article
Research progress on medical image dataset expansion methods

Journal of Biomedical Engineering

Frontiers and development in live-cell super-resolution fluorescence microscopy

Abstract Full text Figures/Tables Video References Cited by

Previous Article

Next Article

Format

Content