Research on the Effects and Mechanisms of Astragalus and Radix Notoginseng on Renal Ischemia Reperfusion Injury in Rats_West China Medical Journal

Authors：

1. Department of Nephrology, Nanchong Central Hospital, Nanchong, Sichuan 637001, P. R. China; 2. Department of Clinical Medicine, Luzhou Medical College, Luzhou, Sichuan 646000, P. R. China;

Corresponding author：

Keywords：

黄芪三七合剂; 肾缺血再灌注损伤; 活性氧

DOI：

10.7507/1002-0179.20130161

Video：

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目的　观察黄芪三七合剂（A amp;R）对肾缺血再灌注损伤（IRI）大鼠血液活性氧（ROS）变化的影响，探讨其抗IRI损伤的机制。方法　雄性Sprague-Dawley（SD）大鼠30只，随机分为正常组（n=5）、假手术组（SG）（n=5）和IRI 24 h组（n=10），A amp;R组（n=10）。造模：采用微血管夹夹闭双侧肾蒂，22 min后松开动脉夹，用5/0尼龙缝合线缝合腹部。再灌注24 h后将小鼠行麻醉处死。A amp;R组给予A amp;R（3 mL/d），假手术组及IRI 24 h组给予同等体积的生理盐水。采用全自动生化分析仪检测各组大鼠的肾功能，苏木精-伊红染色了解肾脏病理损害，流式细胞仪检测红细胞ROS。结果　IRI 24 h组和A amp;R组肾小管出现不同程度的管腔扩张、变性与坏死，间质炎性细胞浸润、充血水肿等变化。IRI后24 h时，IRI 24 h组、A amp;R组血清尿素氮（BUN）和肌酐（Cr）均高于假手术组、正常组，差异有统计学意义（P＜0.05）；A amp;R组ROS荧光强度阳性率显著低于IRI 24 h组，差异有统计学意义（P＜0.05）。A amp;R组肾小管损伤评分明显低IRI 24 h组（P＜0.05）。相关性分析发现，红细胞ROS荧光强度阳性率与肾小管损伤评分、肌酐、尿素氮水平成正相关（r=0.917，P＜0.01；r=0.897，P＜0.01；r=0.896，P＜0.01）。结论　A amp;R对肾脏缺血再灌注损伤具有明显的保护作用，其机制可能为抑制血液中ROS的活性，从而抑制氧化应激对肾脏的损伤。

Citation： YANG Yue,XIE Xisheng,AI Na,WANG Yanjiang.. Research on the Effects and Mechanisms of Astragalus and Radix Notoginseng on Renal Ischemia Reperfusion Injury in Rats. West China Medical Journal, 2013, 28(4): 500-504. doi: 10.7507/1002-0179.20130161 Copy

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1. 　Nath KA, Norby SM. Reactive oxygen species and acute renal failure[J]. Am J Med, 2000, 109(8): 665-678.
2. 　谢席胜, 冯胜刚, 左川, 等. 黄芪三七合剂对单侧输尿管梗阻大鼠肾间质纤维化的作用研究[J]. 西部医学, 2011, 23(10): 1854-1858.
3. 　史晓萍, 宗阿南, 陶钧, 等.关于善待实验动物的指导性意见的研究[J]. 中国医科大学学报, 2007, 36(4): 95-97.
4. 　Valdes F, Pasaro E, Diaz I, et al. Segmental heterogeneity in Bcl-2, Bcl-xL and Bax expression in rat tubular epithelium after ischemia reperfusion[J]. Nephrology (Carlton), 2008, 13(4): 294-301.
5. 　Hauet T, Mothes D, Goujon JM, et al. Trimetazidine prevents renal injury in the isolated perfused pig kidney exposed to prolonged cold ischemia[J]. Transplantation, 1997, 64(7): 1082-1086.
6. 　Maxhimer JB, Shih HB, Isenberg JS, et al. Thrombospondin-1/CD47 blockade following ischemia-reperfusion injury is tissue protective[J]. Plast Reconstr Surg, 2009, 124(6): 1880-1889.
7. 　Djamali A. Oxidative stress as a common pathway to chronic tubulointerstitial injury in kidney allografts[J]. Am J Physiol Renal Physiol, 2007, 293(2): 445-455.
8. 　Valko M, Leibfritz D, Moncol J, et al. Free radicals and antioxidants in normal physiological functions and human disease[J]. Int J Biochem Cell Biol, 2007, 39(1): 44-84.
9. 　王建新, 梅广林, 胡卫东. 黄芪在肝脏缺血-再灌注损伤中保护作用的研究[J]. 南通大学学报·医学版, 2005, 25(3): 161-163．.
10. 　张可, 李华, 王军. 三七总苷对大鼠缺血再灌注肾损伤的保护作用[J].郑州大学学报·医学版, 2006, 41(6): 1165-1166.
11. 　童琦燕. 牛膝治疗高尿酸血症高脂血症[J]. 辽宁中医杂志, 2003, 30(11): 943.
12. 　王慧铭, 孙炜, 黄素霞 ,等.昆布多糖对大鼠减肥及降血脂作用的实验研究[J]. 中国现代应用药学杂志, 2008, 25(1): 16-19.
13. 　Qiao X, Li RS, Li H, et al. Intermedin protects against renal ischemia-reperfusion injury by inhibition of oxidative stress[J]. Am J Physiol Renal Physiol, 2013, 304(1): 112-119.
14. 　Zheng Y, Lu M, Ma L, et al. Osthole ameliorates renal ischemia- reperfusion injury in rats[J]. J Surg Res, 2013, 4804-4807.

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West China Medical Journal

Research on the Effects and Mechanisms of Astragalus and Radix Notoginseng on Renal Ischemia Reperfusion Injury in Rats

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