Effect of Ultrastructure on Human Ovarian Tissue Cryopreservation with a Novel Vitrification Method_West China Medical Journal

Authors：

XIAO Zhun ¹ , WANG Yan ¹ , LI Lingling ² ,  LI Shangwei. ¹

1. Reproductive Medical Center of West China 2nd University Hospital, Sichuan University, Chengdu, Sichuan 610041, P. R. China; 2. Department of Obstetrics and Gynecology, Sichuan Provincial Hospital for Women and Children, Chengdu, Sichuan 610031, P. R. China.;

Corresponding author：

LI Shangwei., Email: xiaozhunok@163.com

Keywords：

玻璃化冷冻; 人卵巢组织; 超微结构; 间质细胞

DOI：

10.7507/1002-0179.20130180

Video：

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目的　探讨新型玻璃化冷冻法对人卵巢组织超微结构的保存效果，尤其是对卵巢间质细胞的保存效果。方法　收集2007年6月－2009年1月在我院行妇科手术的患者卵巢组织共8例，使用新型玻璃化冷冻法和慢速冷冻法保存，比较两种冷冻方法对于始基卵泡卵母细胞、颗粒细胞、卵泡周围间质细胞超微结构保存效果。结果　对于始基卵泡卵母细胞和颗粒细胞，发现两种冷冻方法之间无显著差异（P＞0.05）；在间质细胞保存中，新型玻璃化冷冻法与慢速冷冻法相比较，正常间质细胞百分率增加（P＜0.05）。结论　新型玻璃化冷冻法与慢速冷冻法相比能更好地保存卵泡周围间质细胞，在深低温保存人卵巢组织中具有较广阔的应用前景。

Citation： XIAO Zhun,WANG Yan,LI Lingling,LI Shangwei.. Effect of Ultrastructure on Human Ovarian Tissue Cryopreservation with a Novel Vitrification Method. West China Medical Journal, 2013, 28(4): 567-570. doi: 10.7507/1002-0179.20130180 Copy

1.	Amorim CA, Curaba M, van Langendonckt A, et al. Vitrification as an alternative means of cryopreserving ovarian tissue[J]. Reprod Biomed. Online, 2011, 23(2): 160-186.
2.	Chang HJ, Moon JH, Lee JR, et al. Optimal condition of vitrification method for cryopreservation of human ovarian cortical tissues[J]. J Obstet Gynaecol, 2011, 37(8): 1092-1101.
3.	Zhou XH, Wu YJ, Shi J, et al. Cryopreservation of human ovarian tissue: comparison of novel direct cover vitrification and conventional vitrification[J]. Cryobiology, 2010, 60 (2): 101-105.
4.	Chen SU, Chen CL, Wu MY, et al. Novel direct cover vitrification for cryopreservation of ovarian tissues increases follicle viability and pregnancy capability in mice[J]. Hum Reprod, 2006, 21(11): 2794-2800.
5.	Hasegawa A, HamadaY, Mehandjiev T, et al. In vitro growth and maturation as well as fertilization of mouse preantral oocytes from vitrified ovaries[J]. Fertil Steril, 2004, 81(Suppl 1): 824-830.
6.	Yeoman RR., Wolf DP, and Lee D M. Coculture of monkey ovarian tissue increases survival after vitrification and slow-rate freezing[J]. Fertil Steril, 2005, 83(4): 1248-1254.
7.	Wang Y, Xiao Z, Li L, et al. Novel needle immersed vitrification: a practical and convenient method with potential advantages in mouse and human ovarian tissue cryopreservation[J]. Hum Reprod, 2008, 23(10): 2256-2265.
8.	Gosden RG, Baird DT, Wade JC, et al. Restoration of fertility to oophorectomized sheep by ovarian autografts stored at -196 degrees C[J]. Hum Reprod, 1994, 9(4): 597-603.
9.	Vajta G, Holm P, Kuwayama M.,et al. Open pulled straw(OPS) vitrification, a new way to reduce cryoinjuries of bovine ova and embryos[J]. Mol Reprod Dev, 1998, 51(1): 53-58.
10.	Antinori M, Licata E, Dani G, et al. Cryotop vitrification of human oocytes results in high survival rate and healthy deliveries[J]. Reprod Biomed Online, 2007, 14(1): 72-79.
11.	Valbuena D, Póo ME, Aguilar-Gallardo C, et al. Comparison of Cryotip vs. Cryotop for mouse and human blastomere vitrification[J]. Fertil Steril, 2012, 97(1): 209-217.
12.	Huang LL, Mo YQ, Wang WJ, et al. Cryopreservation of human ovarian tissue by solid-surface vitrification[J]. Eur J Obstet Gynecol Reprod Biol, 2008, 139(2): 193-198.
13.	Isachenko E, Isachenko V, Rahimi G, et al. Cryopreservation of human ovarian tissue by direct plunging into liquid nitrogen[J]. Eur J Obstet Gynecol Reprod Biol, 2003, 108(2): 186-193.
14.	Hovatta O, Wright C, Krausz T, et al. Human primordial, primary and secondary ovarian follicles in long-term culture: effect of partial isolation[J]. Hum Reprod, 1999, 14(10): 2519-2524.
15.	Liu J, Van Der Elst J, Van Den Broecke R, et al. Maturation of mouse primordial follicles by combination of grafting and in vitro culture[J]. Biol Reprod, 2000, 62(5): 1218-1223.
16.	M Xu, SL Barrett, E West-Farrell, et al. In vitro grown human ovarian follicles from cancer patients support oocyte growth[J]. Hum Reprod, 2009, 24(10): 1032-1036.

1. 　Amorim CA, Curaba M, van Langendonckt A, et al. Vitrification as an alternative means of cryopreserving ovarian tissue[J]. Reprod Biomed. Online, 2011, 23(2): 160-186.
2. 　Chang HJ, Moon JH, Lee JR, et al. Optimal condition of vitrification method for cryopreservation of human ovarian cortical tissues[J]. J Obstet Gynaecol, 2011, 37(8): 1092-1101.
3. 　Zhou XH, Wu YJ, Shi J, et al. Cryopreservation of human ovarian tissue: comparison of novel direct cover vitrification and conventional vitrification[J]. Cryobiology, 2010, 60 (2): 101-105.
4. 　Chen SU, Chen CL, Wu MY, et al. Novel direct cover vitrification for cryopreservation of ovarian tissues increases follicle viability and pregnancy capability in mice[J]. Hum Reprod, 2006, 21(11): 2794-2800.
5. 　Hasegawa A, HamadaY, Mehandjiev T, et al. In vitro growth and maturation as well as fertilization of mouse preantral oocytes from vitrified ovaries[J]. Fertil Steril, 2004, 81(Suppl 1): 824-830.
6. 　Yeoman RR., Wolf DP, and Lee D M. Coculture of monkey ovarian tissue increases survival after vitrification and slow-rate freezing[J]. Fertil Steril, 2005, 83(4): 1248-1254.
7. 　Wang Y, Xiao Z, Li L, et al. Novel needle immersed vitrification: a practical and convenient method with potential advantages in mouse and human ovarian tissue cryopreservation[J]. Hum Reprod, 2008, 23(10): 2256-2265.
8. 　Gosden RG, Baird DT, Wade JC, et al. Restoration of fertility to oophorectomized sheep by ovarian autografts stored at -196 degrees C[J]. Hum Reprod, 1994, 9(4): 597-603.
9. 　Vajta G, Holm P, Kuwayama M.,et al. Open pulled straw(OPS) vitrification, a new way to reduce cryoinjuries of bovine ova and embryos[J]. Mol Reprod Dev, 1998, 51(1): 53-58.
10. 　Antinori M, Licata E, Dani G, et al. Cryotop vitrification of human oocytes results in high survival rate and healthy deliveries[J]. Reprod Biomed Online, 2007, 14(1): 72-79.
11. 　Valbuena D, Póo ME, Aguilar-Gallardo C, et al. Comparison of Cryotip vs. Cryotop for mouse and human blastomere vitrification[J]. Fertil Steril, 2012, 97(1): 209-217.
12. 　Huang LL, Mo YQ, Wang WJ, et al. Cryopreservation of human ovarian tissue by solid-surface vitrification[J]. Eur J Obstet Gynecol Reprod Biol, 2008, 139(2): 193-198.
13. 　Isachenko E, Isachenko V, Rahimi G, et al. Cryopreservation of human ovarian tissue by direct plunging into liquid nitrogen[J]. Eur J Obstet Gynecol Reprod Biol, 2003, 108(2): 186-193.
14. 　Hovatta O, Wright C, Krausz T, et al. Human primordial, primary and secondary ovarian follicles in long-term culture: effect of partial isolation[J]. Hum Reprod, 1999, 14(10): 2519-2524.
15. 　Liu J, Van Der Elst J, Van Den Broecke R, et al. Maturation of mouse primordial follicles by combination of grafting and in vitro culture[J]. Biol Reprod, 2000, 62(5): 1218-1223.
16. 　M Xu, SL Barrett, E West-Farrell, et al. In vitro grown human ovarian follicles from cancer patients support oocyte growth[J]. Hum Reprod, 2009, 24(10): 1032-1036.

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Effect of Ultrastructure on Human Ovarian Tissue Cryopreservation with a Novel Vitrification Method

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