Effect of Down-regulated ATM Expression on Radiosensitivity for Laryngeal Squamous Cell Carcinoma in Nude Mice_West China Medical Journal

Authors：

1. Department of Pathology, North Sichuan Medical College & Department of Pathology, Affiliated Hospital of North Sichuan Medical College; 1. Department of Otolaryngology-Head and Neck Surgery, Nanchong Central Hospital, Second Clinical Medical College of North Sichuan Medical College; 3. Institute of Molecular Biology, North Sichuan Medical College; Nanchong, Sichuan 637007, P. R. China;

Corresponding author：

Keywords：

ATM基因; 反义ATM; 喉鳞状细胞癌

DOI：

10.7507/1002-0179.20130226

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目的　探讨反义ATM多核苷酸（AS-Lipo）作用于裸鼠移植瘤对喉鳞状细胞癌(Hep-2)放射治疗敏感性的影响。方法　用克隆生存率来检测喉鳞状细胞癌放射治疗后的生存能力；用流式细胞仪来分析喉癌细胞的凋亡情况；用Tunel染色法检测各组瘤体中细胞凋亡情况。结果　在同等剂量的放射治疗下，AS-Lipo组的生存分数最低。接受4 Gy放射治疗后，行流式细胞仪检测，结果发现AS-Lipo组的凋亡率为（30.7 ± 1.31）%，远高于转染Sen-Lipo、Mis-Lipo、Lipo组。ATM AS-Lipo+放疗组的细胞凋亡数显著高于单独放射治疗组；ATM AS-Lipo+放射治疗组裸鼠瘤体内的凋亡指数为（17.12 ± 4.2）%，与其他组比较差异有统计学意义。结论　反义ATM多核苷酸增强了裸鼠喉鳞状细胞癌对放射治疗的敏感性。

Citation： LI Li,FENG Jun,WANG Chaoli,JING Baoqian.. Effect of Down-regulated ATM Expression on Radiosensitivity for Laryngeal Squamous Cell Carcinoma in Nude Mice. West China Medical Journal, 2013, 28(5): 728-731. doi: 10.7507/1002-0179.20130226 Copy

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1. 　Hristov B, Bajaj GK. Radiotherapeutic management of laryngeal carcinoma[J]. Otolaryngologic clinics of North America, 2008, 41(4): 715-740.
2. 　Li L, Story M, Legerski RJ. Cellular responses to ionizing radiation damage [J]. Int J Radiat Oncol Biol Phys, 2001, 49(4): 1157-1162.
3. 　Christopher WJ, Dariel PG, Cecile VRT. Aberrant recombination involving the granzyme locus occurs in Atm-/-T-cell lymphomas[J]. Human Molecular Genetics, 2005, 14(18): 2671-2684.
4. 　Helt CE, Cliby WA, Keng PC, et al. Ataxia telangiectasia mutated (ATM) and ATM and Rad3-related protein exhibit selective target specificities in response to different forms of DNA damage[J]. J BiolChem, 2005, 280(2): 1186-92.
5. 　Kastan MB, Lim DS. The many substrates and functions of ATM[J]. Nat RevMol Cell Biol, 2000, 1(2): 179-186.
6. 　Chong MJ, Murray MR, Gosink EC, et al. Atm and Bax cooperate in ionizing radiation induced apoptosis in the central nervous system[J]. Proc Natl Acad Sci USA., 2000, 97(2): 889-894.
7. 　Borges HL, Chao C, Xu Y, et al. Radiation-induced apoptosis in developing mouse retina exhibits dose-dependent requirement for ATM phosphorylation of p53[J]. Cell Death Differ, 2004, 11(5): 494-502.
8. 　江继平, 冯俊, 唐嗣泉, 等．反义ATM在体外对喉鳞状细胞癌ATM蛋白表达影响的研究[J]. 川北医学院学报, 2011, 26(5): 394-396.
9. 　李丽, 冯俊, 王朝莉, 等．反义ATM对喉鳞状细胞癌ATM mRNA、蛋白影响的体外研究[J]．川北医学院学报, 2012, 27(5): 438-440.

West China Medical Journal

Effect of Down-regulated ATM Expression on Radiosensitivity for Laryngeal Squamous Cell Carcinoma in Nude Mice

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