Effect of Different Doses of Fospropofol Disodium Post-treatment on Liver in Rats with Hepatic Ischemia-reperfusion Injury_West China Medical Journal

Authors：

Department of Anesthesiology, West China Hospital, Sichuan University, Chengdu, Sichuan 610041, P. R. China;

Corresponding author：

Keywords：

磷丙泊酚钠; 大鼠; 肝脏缺血再灌注损伤; 后处理; 保护作用

DOI：

10.7507/1002-0179.20130229

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目的　探讨磷丙泊酚钠后处理对大鼠肝脏缺血再灌注损伤的影响及是否呈剂量相关性。方法　40只SD大鼠随机分为5组（每组n=8），即：假手术组（SP组）、生理盐水后处理组（NS组）、丙泊酚后处理组（PRO组）、低剂量磷丙泊酚钠[6 mg/（kg·h）]后处理组（LFOS组）、高剂量磷丙泊酚钠[12 mg/（kg·h）]后处理组（HFOS组）。除SP组外，其余4组在肝脏缺血60 min后给予药物后处理直至手术结束。在缺血60 min、再灌注60 min和120 min时采集血样，测定血清中丙氨酸氨基转移酶（ALT）、天门冬氨酸氨基转移酶（AST）、乳酸脱氢酶（ LDH）含量；在灌注120 min时取大鼠肝左外叶，用于HE染色，观察肝脏的形态学改变。结果　与NS组相比，SP组、PRO组、LFOS组和HFOS组血清中的ALT、AST、LDH值明显降低（P＜0.05）；与SP组比较，PRO组、LFOS组、HFOS组和NS组的ALT、AST、LDH值升高（P＜0.05）；与PRO组比较，LFOS组的ALT、AST和LDH值差异无统计学意义（P＞0.05），HFOS组的ALT、AST和LDH值降低（P＜0.05）；LFOS组与HFOS组比较，HFOS组的ALT、AST和LDH值降低更为明显（P＜0.05）。结论　磷丙泊酚钠后处理对大鼠肝脏缺血再灌注损伤具有保护作用，且高剂量磷丙泊酚钠[12 mg/（kg·h）]的保护作用更为明显，保护作用存在剂量依赖性。

Citation： HUANG Qinghua,LUO Chaozhi.. Effect of Different Doses of Fospropofol Disodium Post-treatment on Liver in Rats with Hepatic Ischemia-reperfusion Injury. West China Medical Journal, 2013, 28(5): 741-745. doi: 10.7507/1002-0179.20130229 Copy

1.	李翠, 王国林, 王海云, 等. 丙泊酚后处理对大鼠脑缺血再灌注损伤的长时程保护作用[J]. 天津医药, 2011, 39(4): 357-360.
2.	文志廷, 王国林, 王海云, 等. 异丙酚后处理对大鼠脑缺血再灌注损伤的影响:长期观察[J]. 中华麻醉学杂志, 2012, 32(4): 411-415.
3.	王海云, 王国林, 于泳浩, 等. 异丙酚后处理对颅内动脉瘤夹闭术患者脑缺血再灌注时脑组织抗氧化作用的影响[J]. 中华麻醉学杂志, 2010, 30(2): 188-191.
4.	陈岗, 米卫东, 张宏. 不同剂量异丙酚对老龄大鼠慢性缺血性脑损伤后认知功能的影响[J]. 中华麻醉学杂志, 2011, 31(6): 720-722.
5.	Kohli V, Selzner M, Madden JF, et al. Endothelial cell and hepatocyte deaths occur by apoptosis after ischemia-reperfusion injury in the rat liver[J]. Transplantation, 1999, 67(8): 1099-1105.
6.	de La Cruz JP, Sedeño G, Carmona JA, et al. The in vitro effects of propofol on tissular oxidative stress in the rat[J]. Anesthesia & Analgesia, 1998, 87(5): 1141-1146.
7.	Zhao ZQ, Corvera JS, Halkos ME, et al. Inhibition of myocardial injury by ischemic Postcontioning during reperfusion: comparison with ischemic preconditionlng[J]. Am J Physiol Heart Circ Physiol, 2003, 285(2): H579-588.
8.	黄从刚, 曾秋棠, 李睿, 等. 米诺环素后处理急性心肌梗死再灌注的临床观察[J]. 中华急诊医学杂志, 2012, 21(6): 651-654.
9.	Tsutsumi YM, Yokoyama T, Horikawa Y, et al. Reactive oxygen species trigger ischemic and pharmacological postconditioning: in vivo and in vitro characterization [J]. Life Sci, 2007, 81(15): 1223-1227.
10.	Obal D, Dettwiler S, Favoccia C, et al. The influence of mitochondrial KATP-channels in the cardio-protection of preconditioning and postconditioning by sevoflurane in the rat in vivo[J]. Anesth Analg, 2005, 101(5): 1252-1260.
11.	Dwyhimy DI, Fleming NW, Brodkin IG, et al. Anesthetic preconditioning combined with postconditioning offers no additional benefit over preconditioning or postconditioning alone[J]. Anesth Analg，2007, 105(2): 316-324．.
12.	Ozturk E, Demirbilek S, Kadir But A, et a1. Antioxidant properties of propofol and erythropoietin after closed head injury in rats[J]. Prog Neuroschopharmcol Bio Psychiatry, 2005, 29(6): 922-927.
13.	Boisset S, Steghens JP, Favetta P, et a1. Relative antioxidant capacities of propofol and main metabolites[J]. Arch Toxicol, 2004, 78(11): 635-642.
14.	蔡英敏, 薛荣亮, 马小亚, 等. 不同镇静剂量异丙酚对局灶性脑缺血损伤的保护机制[J]. 西安医科大学学报, 2002, 23(1): 59-61.
15.	郭鸿雁, 王月兰, 安世芝. 不同剂量异丙酚对大鼠缺血再灌注肝脏的抗氧化作用[J]. 中华麻醉学杂志, 2005, 25(9): 701-702.
16.	Atlas G. Fospropofol: Is there an infusion regimen for propofol equivalence?[J]. J Anaesthesiol Clin Pharmacol, 2011, 27(3): 303-306.
17.	来源. 小剂量异丙酚对内毒素孵育兔离体肺动脉、体动脉血管环张力的影响[D]. 中国医科大学, 2009.
18.	孙玥, 曹定睿. 异丙酚后处理对大鼠肝缺血再灌注后血清TNF-α和IL-10水平的影响[J]. 中国医疗前沿, 2011, 6(6): 35-36, 42.
19.	吴新军, 王俊科, 裴凌. 不同剂量异丙酚对大鼠小肠缺血/再灌注后氧自由基所致肺损伤的保护作用[J]. 中华麻醉学杂志, 2003, 23(2): 148-149.
20.	李侠, 李元海, 李磊, 等. 异丙酚对大鼠肝脏缺血再灌注损伤的保护作用[J].安徽医科大学学报, 2007, 42(1): 48-50.
21.	Parke TJ, Stevens JE, Rice AS, et al. Metabolic acidosis and fatal myocardial failure after propofol infusion in children: five case reports[J]. BMJ, 1992, 305(6854): 613-616.
22.	Hatch DJ. Propofol-infusion syndrome in children[J]. Lancet, 1999, 353(9159): 1117-1118.
23.	Fudickar A, Bein B, Tonner PH. Propofol infusion syndrome in anesthesia and intensive care medicine[J]. Curr Opin Anaesthesiol, 2006, 19(4): 406-410.
24.	Patwardhan A, Edelmayer R, Annabi E, et al. Receptor specificity defines algogenic properties of propofol and fospropofol[J]. Anesth Analg, 2012, 115(4): 837-840.

1. 　李翠, 王国林, 王海云, 等. 丙泊酚后处理对大鼠脑缺血再灌注损伤的长时程保护作用[J]. 天津医药, 2011, 39(4): 357-360.
2. 　文志廷, 王国林, 王海云, 等. 异丙酚后处理对大鼠脑缺血再灌注损伤的影响:长期观察[J]. 中华麻醉学杂志, 2012, 32(4): 411-415.
3. 　王海云, 王国林, 于泳浩, 等. 异丙酚后处理对颅内动脉瘤夹闭术患者脑缺血再灌注时脑组织抗氧化作用的影响[J]. 中华麻醉学杂志, 2010, 30(2): 188-191.
4. 　陈岗, 米卫东, 张宏. 不同剂量异丙酚对老龄大鼠慢性缺血性脑损伤后认知功能的影响[J]. 中华麻醉学杂志, 2011, 31(6): 720-722.
5. 　Kohli V, Selzner M, Madden JF, et al. Endothelial cell and hepatocyte deaths occur by apoptosis after ischemia-reperfusion injury in the rat liver[J]. Transplantation, 1999, 67(8): 1099-1105.
6. 　de La Cruz JP, Sedeño G, Carmona JA, et al. The in vitro effects of propofol on tissular oxidative stress in the rat[J]. Anesthesia & Analgesia, 1998, 87(5): 1141-1146.
7. 　Zhao ZQ, Corvera JS, Halkos ME, et al. Inhibition of myocardial injury by ischemic Postcontioning during reperfusion: comparison with ischemic preconditionlng[J]. Am J Physiol Heart Circ Physiol, 2003, 285(2): H579-588.
8. 　黄从刚, 曾秋棠, 李睿, 等. 米诺环素后处理急性心肌梗死再灌注的临床观察[J]. 中华急诊医学杂志, 2012, 21(6): 651-654.
9. 　Tsutsumi YM, Yokoyama T, Horikawa Y, et al. Reactive oxygen species trigger ischemic and pharmacological postconditioning: in vivo and in vitro characterization [J]. Life Sci, 2007, 81(15): 1223-1227.
10. 　Obal D, Dettwiler S, Favoccia C, et al. The influence of mitochondrial KATP-channels in the cardio-protection of preconditioning and postconditioning by sevoflurane in the rat in vivo[J]. Anesth Analg, 2005, 101(5): 1252-1260.
11. 　Dwyhimy DI, Fleming NW, Brodkin IG, et al. Anesthetic preconditioning combined with postconditioning offers no additional benefit over preconditioning or postconditioning alone[J]. Anesth Analg，2007, 105(2): 316-324．.
12. 　Ozturk E, Demirbilek S, Kadir But A, et a1. Antioxidant properties of propofol and erythropoietin after closed head injury in rats[J]. Prog Neuroschopharmcol Bio Psychiatry, 2005, 29(6): 922-927.
13. 　Boisset S, Steghens JP, Favetta P, et a1. Relative antioxidant capacities of propofol and main metabolites[J]. Arch Toxicol, 2004, 78(11): 635-642.
14. 　蔡英敏, 薛荣亮, 马小亚, 等. 不同镇静剂量异丙酚对局灶性脑缺血损伤的保护机制[J]. 西安医科大学学报, 2002, 23(1): 59-61.
15. 　郭鸿雁, 王月兰, 安世芝. 不同剂量异丙酚对大鼠缺血再灌注肝脏的抗氧化作用[J]. 中华麻醉学杂志, 2005, 25(9): 701-702.
16. 　Atlas G. Fospropofol: Is there an infusion regimen for propofol equivalence?[J]. J Anaesthesiol Clin Pharmacol, 2011, 27(3): 303-306.
17. 　来源. 小剂量异丙酚对内毒素孵育兔离体肺动脉、体动脉血管环张力的影响[D]. 中国医科大学, 2009.
18. 　孙玥, 曹定睿. 异丙酚后处理对大鼠肝缺血再灌注后血清TNF-α和IL-10水平的影响[J]. 中国医疗前沿, 2011, 6(6): 35-36, 42.
19. 　吴新军, 王俊科, 裴凌. 不同剂量异丙酚对大鼠小肠缺血/再灌注后氧自由基所致肺损伤的保护作用[J]. 中华麻醉学杂志, 2003, 23(2): 148-149.
20. 　李侠, 李元海, 李磊, 等. 异丙酚对大鼠肝脏缺血再灌注损伤的保护作用[J].安徽医科大学学报, 2007, 42(1): 48-50.
21. 　Parke TJ, Stevens JE, Rice AS, et al. Metabolic acidosis and fatal myocardial failure after propofol infusion in children: five case reports[J]. BMJ, 1992, 305(6854): 613-616.
22. 　Hatch DJ. Propofol-infusion syndrome in children[J]. Lancet, 1999, 353(9159): 1117-1118.
23. 　Fudickar A, Bein B, Tonner PH. Propofol infusion syndrome in anesthesia and intensive care medicine[J]. Curr Opin Anaesthesiol, 2006, 19(4): 406-410.
24. 　Patwardhan A, Edelmayer R, Annabi E, et al. Receptor specificity defines algogenic properties of propofol and fospropofol[J]. Anesth Analg, 2012, 115(4): 837-840.

West China Medical Journal

Effect of Different Doses of Fospropofol Disodium Post-treatment on Liver in Rats with Hepatic Ischemia-reperfusion Injury

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