Objective To establish two-dimensional electrophoresis profiles with high resolution and reproducibility from subcellular immortalized human endocervical cell (H8) and cervical cancer cell (Caski), and to identify the differential expressions of subcellular proteins (cytoplasmic, membranous and nuclear proteins).
Methods H8 cells and Caski cells were incubated, and subcellular proteins of H8 cells and Caski cells were extracted and separated by means of immobilized pH gradient-based two-dimensional gel electrophoresis (2-DE). Then the selected differential protein spots were analyzed by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF-MS) and SWISS-PROT database.
Results We obtained well-resolved, reproducible 2-DE patterns; 6 differentially expressed cytoplasmic proteins and 3 differentially expressed membranous proteins and 9 differentially expressed nuclear proteins were defined in 2-DE gels.
Conclusion Subcellular proteins of cervical precancerous lesion and cervical cancer are separated and analyzed by means of 2-DE and MALDI-TOF-MS/MS. There are significant differences between H8 cells and Caski cells. These data may be valuable for research of cervical precancerous lesion and cervical cancer, or as diagnostic markers and therapeutic targets for cervical cancer.
Citation：
SONGDa-ping, LÜYu-yu, ZHAOYong. Subcellular Proteomics Analysis of Immortalized Cervical Cell and Cervical Cancer Cell. West China Medical Journal, 2014, 29(1): 51-53. doi: 10.7507/1002-0179.20140014
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Copyright © the editorial department of West China Medical Journal of West China Medical Publisher. All rights reserved
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- 1. David E. Two-dimensional gel electrophoresis:an overview[J].Trends in Analyt Chem, 2003, 5(22):263-272,.
- 2. 李茂玉. 亚细胞蛋白质组学研究[J]. 中国医师杂志, 2004, 5(2):718-719.
- 3. 张丽萍, 叶能胜. 宫颈癌蛋白质组学研究新进展[J]. 医学综述, 2011, 17(18):2754-2756.
- 4. Haie-Meder C, de Crevoisier R, Bruna A, et al. Concomitant chemoradiation in patients with cervix cancer[J]. Bull Cancer, 2005, 92(12):1032-1038.
- 5. Gorg A, Obermaier C, Boguth G, et al. The current state of two dimensional electrophoresis with immobilized pH gradients[J]. Electrophoresis, 2000, 21(6):1037-1053.
- 6. 宋艳萍, 姜颖, 贺福初. 亚细胞蛋白质组研究进展[J]. 生命科学, 2007, 19(1):104-108.
- 7. Trejo-Becerril C, Pérez-Cárdenas E, Taja-Chayeb L, et al. Membrane proteins in neoplassic and normal uterine cervix[J]. J Exp Cline Cancer Res, 2001, 20(2):231-237.
- 8. 林英姿, 陈仁, 杨文, 等. 人宫颈癌组织及癌旁正常组织蛋白质组的比较研究[J]. 第四军医大学学报, 2007, 28(17):1593-1596.
- 9. 蔡思娜, 刘国炳, 郭晓红, 等. 应用蛋白芯片-飞行质谱技术筛选宫颈癌血清差异蛋白质[J]. 南方医科大学学报, 2009, 29(1):32-35.
- 10. 罗俊华, 高春芳, 魏蓉, 等. 宫颈浸润癌患者血清蛋白质组的特征研究[J]. 解放军医学杂志, 2010, 35(4):433-435.
- 11. 曾亮, 朱红, 邓亚平, 等. 应用蛋白质组学技术筛选宫颈癌临床分期相关蛋白[J]. 现代生物医学进展, 2009, 8(16):3049-3052.
