Research on the Application of UF-1000i Urine Sediment Analyzer in Rapid Urinary Tract Infection Screening by Receiver Operator Characteristic Curve_West China Medical Journal

Authors：

 MAOZhi-gang , WANGXia , JINYong-mei , XIONGMing , ZHENGQin ,  SUJun

Department of Clinical Laboratory, West China Hospital, Sichuan University, Chengdu, Sichuan 610041, P. R. China;

Corresponding author：

SUJun, Email: daisy2667@163.com

Keywords：

Urinary tract infection; UF-1000i urinary sediment analyzer; Receiver operator characteristic curve; Bacterial Count; Rapid diagnosis

DOI：

10.7507/1002-0179.20140333

Video：

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Objective To investigate the feasibility of quantitative detection of WBC count and bacteria count with UF-1000i urinary sediment analyzer in rapid screening for urinary tract infection by receiver operator characteristic (ROC) curve. Methods From August to December 2013, we used quantitative bacterial culture and UF-1000i automatic urine sediment analyzer respectively to examine asepsis urine specimens of 218 patients with suspected urinary tract infection. Among them, there were 95 males and 123 females, with an average age of 54.7 years old. Results Among the 218 urinary samples, 65 were culture positive specimens. With positive urine culture as the gold standard for making ROC curve, the area under ROC curve for WBC count and bacterial numbers by UF-1000i urine sediment analyzer were respectively 0.839 and 0.894. The cut-off values of Youden index for optimal WBC cell count and bacterial count were ≥31.0/μL and 38.8/μL, respectively. When the above numbers were used as cut-off values, the WBC count sensitivity and specificity were 78.3% and 80.4%, the positive likelihood ratio was 3.99, and the negative likelihood ratio was 1.11. And the bacterial count sensitivity and specificity were 84.3% and 80.6%, the positive likelihood ratio was 4.30, and the negative likelihood ratio was 0.80. Conclusion Using white blood cell count ≥31/μL and bacterial count ≥38.8/μL detected by UF-1000i urine sediment analyzer as the cut off values of noninvasive screening indexes has a very important value in screening for urinary tract infection in the early stage, determining whether there is a need for urine culture, and guiding clinical rational application of antibiotics

Citation： MAOZhi-gang, WANGXia, JINYong-mei, XIONGMing, ZHENGQin, SUJun. Research on the Application of UF-1000i Urine Sediment Analyzer in Rapid Urinary Tract Infection Screening by Receiver Operator Characteristic Curve. West China Medical Journal, 2014, 29(6): 1092-1095. doi: 10.7507/1002-0179.20140333 Copy

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10.	陈丽, 张坤, 李月强, 等. Uf-1000i尿沉渣分析仪检测细菌的性能及对尿路感染的筛查价值[J]. 华中科技大学学报·医学版, 2011, 40(3): 354-356, 360.
11.	王盛华, 刘芸, 肖平, 等. 健康人群Sysmex UF-1000i尿液流式分析参考范围的调查[J]. 国际检验医学杂志, 2012, 33(4): 442-443, 455.
12.	邓益斌, 张梁, 邓巧莹, 等. 尿沉渣细菌定量计数与尿液细菌培养比较及其在筛查泌尿系统感染中的临床应用[J]. 中国实验诊断学, 2010, 14(3): 449-450.
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14.	吴华军, 吕青山, 侯史文, 等. UF-1000i尿液分析仪在尿路感染诊断中的应用[J]. 浙江实用医学, 2010, 15(4): 324-326.

1. Graham JC, Galloway A. ACP Best Practice No 167: the laboratory diagnosis of urinary tract infection[J]. J Clin Pathol, 2001, 54(12): 913-919.
2. 杭建峰, 孙朝晖, 石玉玲, 等. Sysmex UF-1000i全自动尿沉渣分析仪的性能分析及临床应用[J]. 检验医学, 2011, 26(2): 108-110.
3. 黄春光, 杨洁飞, 李小平. UF-1000i尿沉渣分析系统在尿路感染快速诊断中的应用[J]. 实验与检验医学, 2009, 27(5): 489-490.
4. 丛玉隆. 尿液沉渣检查标准化的建议[J]. 中华检验医学杂志, 2002, 25(4): 56-57.
5. 陈卫中, 倪宗瓒, 潘晓平, 等. 用ROC曲线确定最佳临界点和可疑值范围[J]. 现代预防医学, 2005, 32(7): 729-731.
6. 石晶, 赵敏, 欧阳金铭. 以UF-100尿分析仪的WBC和细菌定量结果建立阈值筛选尿细菌培养标本[J]. 临床检验杂志, 2008, 26(3): 181-182.
7. Manoni F, Fornasiero L, Ercolin M, et al. Cutoff values for bacteria and leukocytes for urine flow cytometer Sysmex UF-1000i in urinary tract infections[J]. Diagn Microbiol Infect Dis, 2009, 65(2): 103-107.
8. Delanghe JR, Kouri TT, Huber AR, et al. The role of automated urine particle flow cytometry in clinical practice[J]. Clin Chim Acta, 2000, 301(1-2): 1-18.
9. 吴晶晶, 刘佳, 梅亚宁, 等. 尿路感染病原菌分布及耐药性分析[J]. 南京医科大学学报·自然科学版, 2010, 30(9): 1356-1359.
10. 陈丽, 张坤, 李月强, 等. Uf-1000i尿沉渣分析仪检测细菌的性能及对尿路感染的筛查价值[J]. 华中科技大学学报·医学版, 2011, 40(3): 354-356, 360.
11. 王盛华, 刘芸, 肖平, 等. 健康人群Sysmex UF-1000i尿液流式分析参考范围的调查[J]. 国际检验医学杂志, 2012, 33(4): 442-443, 455.
12. 邓益斌, 张梁, 邓巧莹, 等. 尿沉渣细菌定量计数与尿液细菌培养比较及其在筛查泌尿系统感染中的临床应用[J]. 中国实验诊断学, 2010, 14(3): 449-450.
13. 罗剑, 孙朝晖, 甘燕玲, 等. UF-1000i尿沉渣分析仪对尿路感染早期诊断的价值[J]. 华南国防医学杂志, 2010, 24(1): 61-62.
14. 吴华军, 吕青山, 侯史文, 等. UF-1000i尿液分析仪在尿路感染诊断中的应用[J]. 浙江实用医学, 2010, 15(4): 324-326.

West China Medical Journal

Research on the Application of UF-1000i Urine Sediment Analyzer in Rapid Urinary Tract Infection Screening by Receiver Operator Characteristic Curve

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