The Effect of Post-conditioning with Fospropofol Disodium on Hepatic Ischemia-reperfusion and Its Influence on the Expression of bcl-2/bax Protein in Rats_West China Medical Journal

Authors：

 HEYin ,  LUOChao-zhi

Department of Anesthesiology, West China Hospital, Sichuan University, Chengdu, Sichuan 610041, P. R. China;

Corresponding author：

LUOChao-zhi, Email: chaozhil@aliyun.com

Keywords：

Fospropofol disodium; Hepatic ischemia-reperfusion; Post-conditioning; Protective effects; bcl-2/bax protein; Rats

DOI：

10.7507/1002-0179.20140382

Video：

Export PDF Favorites Scan Get Citation

Abstract Full text Figures/Tables Video References Cited by

Objective To investigate the effect of post-conditioning with fospropofol disodium on hepatic ischemiareperfusion (I/R) and its possible mechanism in rats. Methods Forty-eight Sprague-Dawley rats were randomly divided into four groups, including sham group (S), control group (C), propofol group (P) and fospropofol disodium group (F). According to the different periods after reperfusion, each group was further divided into 2-hour and 4-hour reperfusion subgroups respectively (n=6 in each subgroup), named S_2h, C_2h, P_2h, and F_2h subgroups and S_4h, C_4h, P_4h, and F_4h subgroups. The livers of rats were reperfused after hepatic ischemia for one hour. In the beginning of reperfusion, normal saline was infused intravenously in group S and group C continuously, propofol was infused intravenously in group P continuously, fospropofol disodium was infused continuously in group F. The blood was sampled at the end of ischemia and reperfusion for assay of alanine aminotransferase (ALT) and aspartate aminotransferase (AST). The bcl-2 and bax protein contents in liver tissue were detected by immunohistochemical analysis, and liver samples were stained with hematoxylin-eosine for histological observation and damage degree evaluation by counting the proportion of necrosis cells. Results The activity of ALT and AST, the rate of necrosis cells and the amount of bcl-2 and bax protein after reperfusion in group C, group P and group F were higher than those in group S at matched reperfusion time points (P<0.05). The activity of ALT and AST, the proportion of necrosis cells and bax protein contents decreased in group P and group F, compared with group C at the same reperfusion time points, while the contents of bcl-2 protein were significantly increased (P<0.05). Conclusion Fospropofol disodium can alleviate hepatic injury induced by ischemia-reperfusion in rats, in which the bcl-2 and bax protein may play important roles.

Citation： HEYin, LUOChao-zhi. The Effect of Post-conditioning with Fospropofol Disodium on Hepatic Ischemia-reperfusion and Its Influence on the Expression of bcl-2/bax Protein in Rats. West China Medical Journal, 2014, 29(7): 1242-1247. doi: 10.7507/1002-0179.20140382 Copy

1.	肖峰, 周晓清, 李元涛, 等. 异丙酚对大鼠缺血再灌注海马区Bax和Bcl-2表达的影响[J]. 华中科技大学学报·医学版, 2010, 39(1):113-115, 119.
2.	李锦, 王莉莉, 李树人. 丙泊酚对大鼠肾缺血再灌注损伤细胞凋亡信号通路的影响[J]. 中国组织工程研究与临床康复, 2009, 13(31):6013-6017.
3.	冯春生, 麻海春, 岳云, 等. 异丙酚对大鼠局灶性脑缺血再灌注时NF-κB活化和Bcl-2、Caspase-3基因表达的影响[J]. 中华麻醉学杂志, 2006, 26(5):456-459.
4.	刘宇, 罗朝志. 磷丙泊酚钠对大鼠在体肝脏缺血再灌注损伤的保护作用[J]. 四川大学学报·医学版, 2011, 42(2):231-233.
5.	张琰, 冷玉芳, 方七五. 异丙酚后处理联合缺血后处理对大鼠肝脏缺血再灌注损伤的影响[J]. 中华麻醉学杂志, 2010, 30(1):86-89.
6.	元文勇, 余伟平, 叶启发, 等. 大鼠肝脏缺血再灌注损伤程度判定指标的选择[J]. 中国普通外科杂志, 2008, 17(7):650-653.
7.	王泽华, 殷振奎, 黄海波, 等. 丙泊酚对急性肾缺血再灌注过程脂质过氧化损伤的保护作用[J]. 临床麻醉学杂志, 2001, 17(11):619-621.
8.	Atlas G. Fospropofol:is there an infusion regimen for propofol equivalence?[J]. J Anaesthesiol Clin Pharmacol, 2011, 27(3):303-306.
9.	Chu ZL, Mckinsey T, Liu L, et al. Suppression of tumor necrosis factor-induced cell death by inhibitor of apoptosis c-IAP2 is under NF-κB control[J]. Proc Nati Acad Sci USA, 1997, 94(19):10057-10062.
10.	Deng WL, Wang D, Gosmanova E, et al. LPA protects intestinal epithelial cells from apoptosis by inhibiting the mitochondrial pathway[J]. Am J Physiol Gastrointest Liver Physiol, 2003, 284(5):G821-G829.
11.	Guiral JS, Nleci TJ, Farhood A, et al. Mechanism of Celt death during warm hepatic ischemia-reperfmion in rats:apoptosis or necrosis?[J]. Hepatology, 2001, 33(2):397-405.
12.	Yamabe K, Shimizu S, Kamiike W, et al. Prevention of hypoxic liver cell necrosis by in vivo human bcl-2 gene transfection[J]. Biochem Biophys Res Commun, 1998, 243(1):217-223.
13.	Kane DJ, Ord T, Anton R, et al. Expression of bcl-2 inhibits necrotic neural cell death[J]. J Neurosci Res, 1995, 40(2):269-275.
14.	张玮玮, 郭永清. 丙泊酚对大鼠肝脏缺血再灌注损伤Bcl-2和P53表达的影响[J]. 山西医药杂志, 2008, 37(19):892-894.
15.	Vollmar B, Glasz J, Leiderer R, et al. Hepatic microcirculatory perfusion failure is a determinant for liver dysfunction in warm ischemia-reperfusion[J]. Ampatol, 1994, 145(6):1421-1423.
16.	Jaeschke H. Molecular mechanisms of hepatic ischemia-reperfusion injury and preconditioning[J]. Am J Physiol Gastrointest Liver Physiol, 2003, 284(1):G15-G26.
17.	袁苑, 曹定睿, 殷渊. 丙泊酚对大鼠肠缺血再灌注时肝损伤及Bcl-2、Bax蛋白表达的影响[J]. 山西医科大学学报, 2008, 39(9):791-793.
18.	Campion ME, Gan TJ. Fospropofol disodium for sedation[J]. Drugs Today, 2009, 45(8):567-576.

1. 肖峰, 周晓清, 李元涛, 等. 异丙酚对大鼠缺血再灌注海马区Bax和Bcl-2表达的影响[J]. 华中科技大学学报·医学版, 2010, 39(1):113-115, 119.
2. 李锦, 王莉莉, 李树人. 丙泊酚对大鼠肾缺血再灌注损伤细胞凋亡信号通路的影响[J]. 中国组织工程研究与临床康复, 2009, 13(31):6013-6017.
3. 冯春生, 麻海春, 岳云, 等. 异丙酚对大鼠局灶性脑缺血再灌注时NF-κB活化和Bcl-2、Caspase-3基因表达的影响[J]. 中华麻醉学杂志, 2006, 26(5):456-459.
4. 刘宇, 罗朝志. 磷丙泊酚钠对大鼠在体肝脏缺血再灌注损伤的保护作用[J]. 四川大学学报·医学版, 2011, 42(2):231-233.
5. 张琰, 冷玉芳, 方七五. 异丙酚后处理联合缺血后处理对大鼠肝脏缺血再灌注损伤的影响[J]. 中华麻醉学杂志, 2010, 30(1):86-89.
6. 元文勇, 余伟平, 叶启发, 等. 大鼠肝脏缺血再灌注损伤程度判定指标的选择[J]. 中国普通外科杂志, 2008, 17(7):650-653.
7. 王泽华, 殷振奎, 黄海波, 等. 丙泊酚对急性肾缺血再灌注过程脂质过氧化损伤的保护作用[J]. 临床麻醉学杂志, 2001, 17(11):619-621.
8. Atlas G. Fospropofol:is there an infusion regimen for propofol equivalence?[J]. J Anaesthesiol Clin Pharmacol, 2011, 27(3):303-306.
9. Chu ZL, Mckinsey T, Liu L, et al. Suppression of tumor necrosis factor-induced cell death by inhibitor of apoptosis c-IAP2 is under NF-κB control[J]. Proc Nati Acad Sci USA, 1997, 94(19):10057-10062.
10. Deng WL, Wang D, Gosmanova E, et al. LPA protects intestinal epithelial cells from apoptosis by inhibiting the mitochondrial pathway[J]. Am J Physiol Gastrointest Liver Physiol, 2003, 284(5):G821-G829.
11. Guiral JS, Nleci TJ, Farhood A, et al. Mechanism of Celt death during warm hepatic ischemia-reperfmion in rats:apoptosis or necrosis?[J]. Hepatology, 2001, 33(2):397-405.
12. Yamabe K, Shimizu S, Kamiike W, et al. Prevention of hypoxic liver cell necrosis by in vivo human bcl-2 gene transfection[J]. Biochem Biophys Res Commun, 1998, 243(1):217-223.
13. Kane DJ, Ord T, Anton R, et al. Expression of bcl-2 inhibits necrotic neural cell death[J]. J Neurosci Res, 1995, 40(2):269-275.
14. 张玮玮, 郭永清. 丙泊酚对大鼠肝脏缺血再灌注损伤Bcl-2和P53表达的影响[J]. 山西医药杂志, 2008, 37(19):892-894.
15. Vollmar B, Glasz J, Leiderer R, et al. Hepatic microcirculatory perfusion failure is a determinant for liver dysfunction in warm ischemia-reperfusion[J]. Ampatol, 1994, 145(6):1421-1423.
16. Jaeschke H. Molecular mechanisms of hepatic ischemia-reperfusion injury and preconditioning[J]. Am J Physiol Gastrointest Liver Physiol, 2003, 284(1):G15-G26.
17. 袁苑, 曹定睿, 殷渊. 丙泊酚对大鼠肠缺血再灌注时肝损伤及Bcl-2、Bax蛋白表达的影响[J]. 山西医科大学学报, 2008, 39(9):791-793.
18. Campion ME, Gan TJ. Fospropofol disodium for sedation[J]. Drugs Today, 2009, 45(8):567-576.

West China Medical Journal

The Effect of Post-conditioning with Fospropofol Disodium on Hepatic Ischemia-reperfusion and Its Influence on the Expression of bcl-2/bax Protein in Rats

Abstract Full text Figures/Tables Video References Cited by

Previous Article

Next Article

Format

Content