Comparative Research between Fluorogenic Quantitative Polymerase Chain Reaction and Enzyme-linked Immunosorbent Assay Applied in Diagnosing Enterovirus 71 Infection_West China Medical Journal

Authors：

 YANGXing-lin ¹ , XIONGJin-feng ¹ , LILi ² , WANGYi-guang ¹

1. Department of Laboratory;Guizhou No. 5 People's Hospital of Guiyang, Guiyang, Guizhou 55000, P. R. China;
2. Department of Infection;Guizhou No. 5 People's Hospital of Guiyang, Guiyang, Guizhou 55000, P. R. China;

Corresponding author：

Keywords：

Hand-foot-mouth disease; Enterovirus 71; IgM antibody; Enterovirus 71 nucleic acid

DOI：

10.7507/1002-0179.20140399

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Objective To compare effect of enterovirus (EV) 71 nucleic acid detection and EV71-IgM antibody detection on clinically diagnosis of hand-foot-mouth disease in children. Methods Rectal swabs collected from 1379 children who were clinically diagnosed from April 20, 2011 to September 10, 2011 as suspected patients with the handfoot- mouth disease were detected by fluorogenic quantitative polymerase chain reaction to conduct EV71 nucleic acid detection. Meantime, enzyme-linked immunosorbent assay was used to conduct EV71-IgM antibody detection in serum samples collected from those children. Results In these 1379 cases, 79 had positive EV71 nucleic acids with a positive rate of 5.73%; while 82 cases had positive EV71-IgM antibodies with a positive rate of 5.95%. There were 32 cases with positive EV71 nucleic acid and positive EV71-IgM antibody. The rate of consistent results of two detection methods was 95.2%. The positive rates of two methods had no negligible differences (χ²=0.093, P=0.761). Conclusion Combination of EV71 nucleic acid detection and EV71-IgM antibody detection, can improve the efficiency in diagnosing hand-foot-mouth disease in children and facilitate the protection and diagnosis of the disease.

Citation： YANGXing-lin, XIONGJin-feng, LILi, WANGYi-guang. Comparative Research between Fluorogenic Quantitative Polymerase Chain Reaction and Enzyme-linked Immunosorbent Assay Applied in Diagnosing Enterovirus 71 Infection. West China Medical Journal, 2014, 29(7): 1309-1312. doi: 10.7507/1002-0179.20140399 Copy

1.	Hamaguchi T, Fujisawa H, Sakai K, et al. Acute encephalitis caused by intrafamilial transmission of enterovirus 71 in adult[J]. Emerg Infect Dis, 2008, 14(5):828-830.
2.	Schmidt NJ, Lennette EH, Ho HH. An apparently new enterovirus isolated from patients with disease of the central nervous system[J]. J Infect Dis, 1974, 129(3):304-309.
3.	何家鑫, 王碧锦, 奚月兰, 等. 柯萨奇A16引起"手足口"病流行的特点简报[J]. 中国人兽共患病杂志, 1985, 1(2):36.
4.	毛国顺, 罗玲, 刘晓琳, 等. 手足口病轻症与重症患者临床特征比较[J]. 中华传染病杂志, 2008, 26(7):387-390.
5.	邓莉, 张艳玲. 手足口病的流行与防治[J]. 北京医学, 2008, 30(6):354-355.
6.	赵华, 赵春芳, 陈应琼, 等. 重庆市2009年手足口病病原学分析[J]. 现代预防医学, 2011, 38(12):2384-2385.
7.	李亮, 朱凤才, 史智扬, 等. 江苏省手足口病病原学流行特征分析[J]. 中华预防医学杂志, 2010, 44(7):676-677.
8.	朱冰, 钟家禹, 邝璐, 等. 2010年广州地区儿童肠道病毒71型感染的分子流行病学研究[J]. 中华医院感染学杂志, 2011, 21(12):2401-2403.
9.	杜阳光, 杨晋川, 晏嘉璐, 等. 血清中EV71型IgM抗体检测对手足口病诊断的意义[J]. 中华全科医学, 2011, 9(10):1574-1575.
10.	彭兰芬, 张少丰, 付文金, 等. 肠病毒EV71-IgM抗体与核酸检测在手足口病诊断中的价值[J]. 中国热带医学, 2011, 11(3):274-275.
11.	Tan EL, Chow VT, Quak SH, et al. Development of multiplex real time hybridization probe reverse transcriptase polymerase chain reaction for specific detection and differentiation of Enterovirus 71 and Coxsackievirus A16[J]. Diagn Microbiol Infect Dis, 2008, 61(3):294-301.
12.	杨秀惠, 严延生. 手足口病的病原学研究进展[J]. 传染病信息, 2008, 21(3):129-131.
13.	林裕龙, 温坤, 王压娣, 等. 捕获ELISA法测定抗EV71-IgM抗体用于EV71感染早期诊断[J]. 临床检验杂志, 2012, 30(1):27-28.
14.	谢靖婧, 杨桂林, 刘映霞, 等. 肠道病毒71型手足口病ELISA诊断试剂盒研制与临床应用[J]. 中华检验医学杂志, 2009, 32(11):1262-1265.

1. Hamaguchi T, Fujisawa H, Sakai K, et al. Acute encephalitis caused by intrafamilial transmission of enterovirus 71 in adult[J]. Emerg Infect Dis, 2008, 14(5):828-830.
2. Schmidt NJ, Lennette EH, Ho HH. An apparently new enterovirus isolated from patients with disease of the central nervous system[J]. J Infect Dis, 1974, 129(3):304-309.
3. 何家鑫, 王碧锦, 奚月兰, 等. 柯萨奇A16引起"手足口"病流行的特点简报[J]. 中国人兽共患病杂志, 1985, 1(2):36.
4. 毛国顺, 罗玲, 刘晓琳, 等. 手足口病轻症与重症患者临床特征比较[J]. 中华传染病杂志, 2008, 26(7):387-390.
5. 邓莉, 张艳玲. 手足口病的流行与防治[J]. 北京医学, 2008, 30(6):354-355.
6. 赵华, 赵春芳, 陈应琼, 等. 重庆市2009年手足口病病原学分析[J]. 现代预防医学, 2011, 38(12):2384-2385.
7. 李亮, 朱凤才, 史智扬, 等. 江苏省手足口病病原学流行特征分析[J]. 中华预防医学杂志, 2010, 44(7):676-677.
8. 朱冰, 钟家禹, 邝璐, 等. 2010年广州地区儿童肠道病毒71型感染的分子流行病学研究[J]. 中华医院感染学杂志, 2011, 21(12):2401-2403.
9. 杜阳光, 杨晋川, 晏嘉璐, 等. 血清中EV71型IgM抗体检测对手足口病诊断的意义[J]. 中华全科医学, 2011, 9(10):1574-1575.
10. 彭兰芬, 张少丰, 付文金, 等. 肠病毒EV71-IgM抗体与核酸检测在手足口病诊断中的价值[J]. 中国热带医学, 2011, 11(3):274-275.
11. Tan EL, Chow VT, Quak SH, et al. Development of multiplex real time hybridization probe reverse transcriptase polymerase chain reaction for specific detection and differentiation of Enterovirus 71 and Coxsackievirus A16[J]. Diagn Microbiol Infect Dis, 2008, 61(3):294-301.
12. 杨秀惠, 严延生. 手足口病的病原学研究进展[J]. 传染病信息, 2008, 21(3):129-131.
13. 林裕龙, 温坤, 王压娣, 等. 捕获ELISA法测定抗EV71-IgM抗体用于EV71感染早期诊断[J]. 临床检验杂志, 2012, 30(1):27-28.
14. 谢靖婧, 杨桂林, 刘映霞, 等. 肠道病毒71型手足口病ELISA诊断试剂盒研制与临床应用[J]. 中华检验医学杂志, 2009, 32(11):1262-1265.

West China Medical Journal

Comparative Research between Fluorogenic Quantitative Polymerase Chain Reaction and Enzyme-linked Immunosorbent Assay Applied in Diagnosing Enterovirus 71 Infection

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