Determination of the Concentration of Isonicotinyl Hydrazide in the Hydrothorax of Tuberculosis Patients_West China Medical Journal

Authors：

 ZOUPin-jiao ,  WUJuan , JIANGYun-ping , JINWei-hua , JIANGYan , YUBo-tao , FANKai-hua

Department of Pharmacy, General Hospital of Chengdu Military Region, Chengdu, Sichuan 610083, P. R. China;

Corresponding author：

WUJuan, Email: morriswujuan@126.com

Keywords：

Isonicotinyl hydrazide; High performance liquid chromatography; Tuberculosis patients; Hydrothorax

DOI：

10.7507/1002-0179.20140455

Video：

Export PDF Favorites Scan Get Citation

Abstract Full text Figures/Tables Video References Cited by

Objective To determinate the concentration of isonicotinyl hydrazide (INH) in the hydrothorax and thereby increase the drug concentration of the hydrothorax to enhance the anti-tuberculosis efficacy through experiments. Methods Between February and June 2009, we used high performance liquid chromatography (HPLC) to determinate the concentration of INH in the hydrothorax of tuberculosis (TB) patients. The separation of sample was performed on Agilent ZORBAX SB-C18 (5 μm, 4.6 mm×2.5 mm) column with a fixed sample injection volume of 20 μL. The mobile phase was methanol-water (20︰80) at a flow rate of 1 mL/min. The ultraviolet detective wavelength was set at 254 nm; The column temperature was room temperature. Results In the range of 0.25-10.00 μg/mL (r=0.998 9), moxi-floxacin showed a good linear relation in HPLC. The recoveries of moxi-floxacin at three concentrations (0.5, 5.0, and 10.0 μg/mL) were 97.95%, 100.64%, and 102.84%, respectively, with intra-day relative standard deviation (RSD) at 3.49%, 1.45%, and 2.03%, respectively and intra-day RSD at 3.85%, 5.68%, 4.15%, respectively. Conclusion The measuring method adopted in the experiment can accurately determinate the concentration of INH in the hydrothorax with high sensitivity and exceptional reproducibility.

Citation： ZOUPin-jiao, WUJuan, JIANGYun-ping, JINWei-hua, JIANGYan, YUBo-tao, FANKai-hua. Determination of the Concentration of Isonicotinyl Hydrazide in the Hydrothorax of Tuberculosis Patients. West China Medical Journal, 2014, 29(8): 1483-1486. doi: 10.7507/1002-0179.20140455 Copy

1.	王超, 朱中元. 抗结核病药物的研究进展[J]. 中国热带医学, 2008, 8(4):673-675.
2.	何国钧. 抗结核药物的研究进展[J]. 中国防痨杂志, 1996, 23(3):136-139.
3.	Mitchison DA. Role of individual drugs in the chemotherapy of tuberculosis[J]. Int J Tuberc Lung Dis, 2000, 4(9):796-806.
4.	丁辉. HPLC法测定异烟肼片的含量[J]. 中华医学实践杂志, 2005, 4(8):813-814.
5.	Liapakis IE, Kottakis I, Tzatzarakis MN, et al. Penetration of newer quinolones in the empyema fluid[J]. Eur Respir J, 2004, 24(3):466-740.
6.	Jutte PC, Rutgers SR, Van Altena R. Penetration of isoniazid, rifampicin and pyrazinamide in tuberculous pleural effusion and psoas abscess[J]. Int J Tuberc Lung Dis, 2004, 8(11):1368-1372.
7.	杨德柱, 李文清, 王开平, 等. 高效液相色谱法测定异烟肼固体制剂血药浓度[J]. 黑龙江医药, 1996, 9(2):68-69.
8.	邓立东, 蒋学华, 陈钧, 等. 异福胶囊的人体相对生物利用度研究[J]. 中国现代应用药学, 2004, 21(2):132-136.
9.	黎昌权. 高效液相色谱法测定异烟肼注射液的含量[J]. 中国药业, 2008, 17(8):32-33.
10.	赵伟杰, 段连山, 李惠文. HPLC法测定人血浆异烟肼浓度[J]. 结核病与胸部肿瘤, 1997, 1(2):43-45.
11.	成诗明. 全球结核病流行特征与控制进展[J]. 中国健康教育, 2005, 21(7):499-502.
12.	刘剑君, 幺鸿雁. 我国结核病的流行现状和防治对策[J]. 预防医学文献信息, 2004, 10(3):381-384.
13.	刘会民, 张洁, 张永进. 我院异烟肼血药浓度监测结果分析[J]. 天津药学, 2011, 23(6):27-28.
14.	Liu P, Fu ZZ, Jiang JM, et al. Determination of isoniazid concentration in rabbit vertebrae by isotope tracing technique in conjunction with HPLC[J]. Biomed Chromatogr, 2013, 27(9):1150-1156.
15.	戈朝晖, 王自立, 魏敏吉, 等. RP-HPLC法测定脊柱结核病椎组织中异烟肼的浓度[J]. 脊柱外科杂志, 2008, 6(3):165-168, 190.
16.	徐康平, 李福双, 谭健兵, 等. 川东獐牙菜药材的HPLC指纹图谱初步研究[J]. 中南药学, 2004, 2(1):10-13.
17.	纪兰菊, 保怡, 陈桂琛, 等. 15种獐牙菜属植物中主要药用成分的高效液相色谱测定[J]. 西北植物学报, 2004, 2(7):1298-1302.
18.	曾德贵, 刘玉琴. 异烟肼人体血药浓度测定及药代动力学研究[J]. 中国药业, 2011, 20(3):6-7.

1. 王超, 朱中元. 抗结核病药物的研究进展[J]. 中国热带医学, 2008, 8(4):673-675.
2. 何国钧. 抗结核药物的研究进展[J]. 中国防痨杂志, 1996, 23(3):136-139.
3. Mitchison DA. Role of individual drugs in the chemotherapy of tuberculosis[J]. Int J Tuberc Lung Dis, 2000, 4(9):796-806.
4. 丁辉. HPLC法测定异烟肼片的含量[J]. 中华医学实践杂志, 2005, 4(8):813-814.
5. Liapakis IE, Kottakis I, Tzatzarakis MN, et al. Penetration of newer quinolones in the empyema fluid[J]. Eur Respir J, 2004, 24(3):466-740.
6. Jutte PC, Rutgers SR, Van Altena R. Penetration of isoniazid, rifampicin and pyrazinamide in tuberculous pleural effusion and psoas abscess[J]. Int J Tuberc Lung Dis, 2004, 8(11):1368-1372.
7. 杨德柱, 李文清, 王开平, 等. 高效液相色谱法测定异烟肼固体制剂血药浓度[J]. 黑龙江医药, 1996, 9(2):68-69.
8. 邓立东, 蒋学华, 陈钧, 等. 异福胶囊的人体相对生物利用度研究[J]. 中国现代应用药学, 2004, 21(2):132-136.
9. 黎昌权. 高效液相色谱法测定异烟肼注射液的含量[J]. 中国药业, 2008, 17(8):32-33.
10. 赵伟杰, 段连山, 李惠文. HPLC法测定人血浆异烟肼浓度[J]. 结核病与胸部肿瘤, 1997, 1(2):43-45.
11. 成诗明. 全球结核病流行特征与控制进展[J]. 中国健康教育, 2005, 21(7):499-502.
12. 刘剑君, 幺鸿雁. 我国结核病的流行现状和防治对策[J]. 预防医学文献信息, 2004, 10(3):381-384.
13. 刘会民, 张洁, 张永进. 我院异烟肼血药浓度监测结果分析[J]. 天津药学, 2011, 23(6):27-28.
14. Liu P, Fu ZZ, Jiang JM, et al. Determination of isoniazid concentration in rabbit vertebrae by isotope tracing technique in conjunction with HPLC[J]. Biomed Chromatogr, 2013, 27(9):1150-1156.
15. 戈朝晖, 王自立, 魏敏吉, 等. RP-HPLC法测定脊柱结核病椎组织中异烟肼的浓度[J]. 脊柱外科杂志, 2008, 6(3):165-168, 190.
16. 徐康平, 李福双, 谭健兵, 等. 川东獐牙菜药材的HPLC指纹图谱初步研究[J]. 中南药学, 2004, 2(1):10-13.
17. 纪兰菊, 保怡, 陈桂琛, 等. 15种獐牙菜属植物中主要药用成分的高效液相色谱测定[J]. 西北植物学报, 2004, 2(7):1298-1302.
18. 曾德贵, 刘玉琴. 异烟肼人体血药浓度测定及药代动力学研究[J]. 中国药业, 2011, 20(3):6-7.

West China Medical Journal

Determination of the Concentration of Isonicotinyl Hydrazide in the Hydrothorax of Tuberculosis Patients

Abstract Full text Figures/Tables Video References Cited by

Previous Article

Next Article

Format

Content