Distribution Characteristics and Analysis of Genotype Drug Resistance of Drug-resistant Gene Mutations in Mycobacterium Tuberculosis in Deyang District, Sichuan_West China Medical Journal

Authors：

 YANGJia-hong , ZHANGHao , WANGXiu , CHENGao , CHENXue-bing , LUOWan-rong , JIANGShu-ming

Department of Infectious Diseases, People's Hospital of Deyang City, Deyang, Sichuan 618000, China;

Corresponding author：

Keywords：

Mycobacterium tuberculosis; Drug-resistant genes; Mutations

DOI：

10.7507/1002-0179.20140456

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Objective To explore distribution characteristics of drug-resistant mutations and analyze drug-resistant genotypes in Mycobacterium tuberculosis in Deyang district, Sichuan. Methods A total of 257 patients infected with Mycobacterium tuberculosis and positive for mycobacterium tuberculosis DNA who were detected from February 2010 to March 2013 were included in our research. Drug-resistance mutations were detected and analyzed using gene chip technology combining by polymerase chain reaction (PCR) and reverse dot hybridization (RDB). Results In these 257 pulmonary tuberculosis patients, drug-resistance mutations were detected in 49 with pulmonary tuberculosis. Drug-resistance mutation rate at katG 315, rpsL 43, embB 306 and rpoB 531 (S531L) was 11.67% (30/257), 7.00% (18/257), 4.28% (11/257) and 3.89% (10/257), respectively. In 234 initially treated pulmonary tuberculosis patients, the rate of isoniazid-resistant genotype, rifampicin-resistant genotype, ethambutol-resistant genotype, streptomycin-resistant genotype and multi-drug resistant genotype was 9.83%, 4.27%, 3.42%, 5.13% and 2.99%, respectively. In 23 retreated pulmonary tuberculosis patients, these rates was 52.17%, 26.09%, 13.04%, 43.48% and 13.04%, respectively. Conclusion In Deyang district, Sichuan, drug-resistant genotypes for isoniazid, rifampicin, ethambutol and streptomycin are detected in Mycobacterium tuberculosis. Most of the drug-resistant mutations occur at katG 315, rpsL 43, embB 306 and rpoB 531. The rates of drug-resistant genotypes and multi-drug resistance in initially treated pulmonary tuberculosis patients are lower than those in retreated patients. Multi-drug resistant rate is relatively low in our research.

Citation： YANGJia-hong, ZHANGHao, WANGXiu, CHENGao, CHENXue-bing, LUOWan-rong, JIANGShu-ming. Distribution Characteristics and Analysis of Genotype Drug Resistance of Drug-resistant Gene Mutations in Mycobacterium Tuberculosis in Deyang District, Sichuan. West China Medical Journal, 2014, 29(8): 1487-1490. doi: 10.7507/1002-0179.20140456 Copy

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19.	谭云洪, 易松林, 梁珍. 591株结核分枝杆菌耐药情况分析[J]. 中国防痨杂志, 2009, 31(3):157-159.
20.	邓建平, 李群, 肖体权, 等. 四川省自贡市结核分枝杆菌耐药性监测结果初步分析[J]. 中国预防医学杂志, 2012, 13(9):653-656.
21.	陆军, 叶松, 李朝品, 等. 淮南矿区煤工尘肺结核患者耐多药结核分枝杆菌耐药基因rpoB序列分析[J]. 中华劳动卫生职业病杂志, 2012, 30(8):579-581.
22.	梁伟. 湛江地区住院肺结核患者耐药情况分析[J]. 齐齐哈尔医学院学报, 2010, 31(8):1217-1218.
23.	范大鹏, 张艳, 夏强, 等. 2010-2011年杭州市636例肺结核患者耐药情况分析[J]. 中国防痨杂志, 2012, 34(11):750-752.

1. WHO. Global tuberculosis report 2013[EB/OL]. (2013-11-01)[2014-07-04]. http://www.who.int/tb/publications/global_report/en.
2. WHO. Global tuberculosis control and patient care:a ministerial meeting of high M/XDR-TB burden countries[EB/OL]. (2009-04-01)[2014-07-04]. http://www.who.int/tb_beijingmeeting/en.
3. Guo Y, Zhou Y, Wang C, et al. Rapid, accurate determination of multidrug resistance in M. tuberculosis isolates and sputum using a biochip system[J]. Int J Tuberc Lung Dis, 2009, 13(7):914-920.
4. Zhang Y, Yew WW. Mechanisms of drug resistance in Mycobacterium tuberculosis[J]. Int J Tuberc Lung Dis, 2009, 13(11):1320-1330.
5. Baranauskaite A, Cicenaite J. Molecular characterization of isoniazid-resistant Mycobacterium tuberculosis clinical isolates in Lithuania[J]. Antimicrob Agents Chemother, 2003, 47(6):2009-2011.
6. Rindi L, Bianchi L, Tortoli E, et al. A real-time PCR assay for detection of isoniazid resistance in Mycobacterium tuberculosis clinical isolates[J]. J Microbiol Methods, 2003, 55(3):797-800.
7. Senna SG, Ribeiro M. Mutations in katG, inhA, and ahpC genes of Brazilian isoniazid-resistant isolates of Mycobacterium tuberculosis[J]. J Clin Microbiol, 2003, 41(9):4471-4474.
8. Valim AR, Rossetti ML, Ribeiro MO, et al. Mutations in the rpoB gene of multidrug-resistant Mycobacterium tuberculosis isolates from Brazil[J]. J Clin Microbiol, 2000, 38(8):3119-3122.
9. 魏淑贞, 赵秀芹, 刘志广, 等. 结核分枝杆菌链霉素耐药临床分离株rpsL基因突变特征分析[J]. 中国人兽共患病学报, 2011, 27(12):1097-1100.
10. 梅建华, 雷永良, 王晓光, 等. 结核分枝杆菌异烟肼耐药基因KatG分析[J]. 中国卫生检验杂志, 2010, 20(6):1391-1393.
11. 吴雪琼, 李洪敏, 张俊仙, 等. embB基因突变与结核分支杆菌耐乙胺丁醇的关系[J]. 中华微生物学和免疫学杂志, 2001, 21(6):665.
12. 黄海荣, 马玙, 陈曦, 等. 中国耐利福平结核分支杆菌rpoB基因突变特点[J]. 中华结核和呼吸杂志, 2001, 24(4):231-235.
13. 赵玉玲, 杨洪毅, 马晓光, 等. 河南省耐多药结核分枝杆菌利福平耐药基因rpoB的突变特征[J]. 郑州大学学报·医学版, 2012, 47(2):166-169.
14. 金嘉琳, 张文宏, 翁心华, 等. 华东地区耐药结核分枝杆菌katG基因的变异[J]. 中华传染病杂志, 2006, 24(5):301-305.
15. 张舒林, 孙战强, 伍学强, 等. 结核分枝杆菌对乙胺丁醇耐药突变基因检测方法的建立[J]. 中华检验医学杂志, 2004, 27(3):169-170.
16. 朱爱平, 朱丽, 张修磊. 2009~2012年山东省项目市耐多药结核病可疑患者耐药情况分析[J]. 预防医学论坛, 2012, 18(10):790-791, 793.
17. Grosset J, De Kantor I. Drug-resistant tuberculosis:laboratory issues.World Health Organization recommendations[J]. Tuber Lung Dis, 1994, 75(1):1-7.
18. 魏存乐, 王岐, 王志刚. 223例结核分枝杆菌耐药分析[J]. 中国卫生检验杂志, 2012, 22(9):2222-2224.
19. 谭云洪, 易松林, 梁珍. 591株结核分枝杆菌耐药情况分析[J]. 中国防痨杂志, 2009, 31(3):157-159.
20. 邓建平, 李群, 肖体权, 等. 四川省自贡市结核分枝杆菌耐药性监测结果初步分析[J]. 中国预防医学杂志, 2012, 13(9):653-656.
21. 陆军, 叶松, 李朝品, 等. 淮南矿区煤工尘肺结核患者耐多药结核分枝杆菌耐药基因rpoB序列分析[J]. 中华劳动卫生职业病杂志, 2012, 30(8):579-581.
22. 梁伟. 湛江地区住院肺结核患者耐药情况分析[J]. 齐齐哈尔医学院学报, 2010, 31(8):1217-1218.
23. 范大鹏, 张艳, 夏强, 等. 2010-2011年杭州市636例肺结核患者耐药情况分析[J]. 中国防痨杂志, 2012, 34(11):750-752.

West China Medical Journal

Distribution Characteristics and Analysis of Genotype Drug Resistance of Drug-resistant Gene Mutations in Mycobacterium Tuberculosis in Deyang District, Sichuan

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