N-乙酰氨基葡萄糖转移酶-Ⅴ与恶性肿瘤增殖和侵袭转移的关系_West China Medical Journal

Authors：

 杨娟 ,  陈杰

四川大学华西第二医院妇产科（成都，610041）;

Corresponding author：

陈杰, Email: cjzb@sina.com

Keywords：

N-乙酰氨基葡萄糖转移酶-Ⅴ; 上皮间质转化; 细胞增殖; 肿瘤转移

DOI：

10.7507/1002-0179.20140542

Video：

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Abstract Full text Figures/Tables Video References Cited by

β1，6分支型N-糖链是一类由N-乙酰氨基葡萄糖转移酶-Ⅴ催化生成的糖蛋白，位于细胞表面，其生物活性的变化与细胞的生物行为直接相关，参与细胞增殖，增强肿瘤细胞侵袭、转移能力。因此，对N-乙酰氨基葡萄糖转移酶-Ⅴ的生物功能的全面了解有助于对肿瘤发病机制的进一步把握，从而为恶性肿瘤的治疗提供指导。

Citation： 杨娟, 陈杰. N-乙酰氨基葡萄糖转移酶-Ⅴ与恶性肿瘤增殖和侵袭转移的关系. West China Medical Journal, 2014, 29(9): 1779-1782. doi: 10.7507/1002-0179.20140542 Copy

1.	Dennis JW,Laferté S,Waghorne C,et al.Beta 1-6 branching of Asn-linked oligosaccharides is directly associated with metastasis[J].Science,1987,236(481):582-585.
2.	Granovsky M,Fata J,Pawling J,et al.Suppression of tumor growth and metastasis in Mgat5-deficient mice[J].Nat Med,2000,6(3):306-312.
3.	Saito H,Gu J,Nishikawa A,et al.Organization of the human N-acetylglucosaminyltransferase Ⅴgene[J].Eur J Biochem,1995,233(1):18-26.
4.	Ko JH,Miyoshi E,Noda K,et al.Regulation of the GnT-Ⅴpromoter by transcription factor Ets-1 in various cancer cell lines[J].J Biol Chem,1999,274(33):22941-22948.
5.	Buckhaults P,Chen L,Fregien N,et al.Transcriptional regulation of N-acetylglucosaminyltransferaseⅤ by the src oncogene[J].J Biol Chem,1997,272(31):19575-19581.
6.	Chen L,Zhang W,Fregien N,et al.The her-2/neu oncogene stimulates the transcription of N-acetylglucosaminyltransferase Ⅴ and expression of its cell surface oligosaccharide products[J].Oncogene,1998,17(16):2087-2093.
7.	Taniguchi N,Miyoshi E,Ko JH,et al.Implication of N-acetylglucosaminyltransferases Ⅲ andⅤ in cancer:gene regulation and signaling mechanism[J].Biochim Biophys Acta,1999,1455(2/3):287-300.
8.	Lüthy R,Mclachlan AD,Eisenberg D.Secondary structure-based profiles:use of structure-conserving scoring tables in searching protein sequence databases for structural similarities[J].Proteins,1991,10(3):229-239.
9.	Ihara S,Miyoshi E,Nakahara S,et al.Addition of beta1-6 GlcNAc branching to the oligosaccharide attached to Asn 772 in the serine protease domain of matriptase plays a pivotal role in its stability and resistance against trypsin[J].Glycobiology,2004,14(2):139-146.
10.	Ihara S,Miyoshi E,Ko JH,et al.Prometastatic effect of N-acetylglucosaminyltransferaseⅤ is due to modification and stabilization of active matriptase by adding beta 1-6 GlcNAc branching[J].J Biol Chem,2002,277(19):16960-16967.
11.	Xu Q,Isaji T,Lu Y,et al.Roles of N-acetylglucosaminyltransferase Ⅲ in epithelial-to-mesenchymal transition induced by transforming growth factor β1 (TGF-β1) in epithelial cell lines[J].J Biol Chem,2012,287(20):16563-16574.
12.	Pinho SS,Figueiredo J,Cabral J,et al.E-cadherin and adherens-junctions stability in gastric carcinoma:functional implications of glycosyltransferases involving N-glycan branching biosynthesis,N-acetylglucosaminyltransferases Ⅲ andⅤ[J].Biochim Biophys Acta,2013,1830(3):2690-2700.
13.	Valastyan S,Weinberg RA.Tumor metastasis:molecular insights and evolving paradigms[J].Cell,2011,147(2):275-292.
14.	Gheldof A,Berx G.Cadherins and epithelial-to-mesenchymal transition[J].Prog Mol Biol Transl Sci,2013,116:317-336.
15.	Partridge EA,Le Roy C,Di Guglielmo GM,et al.Regulation of cytokine receptors by Golgi N-glycan processing and endocytosis[J].Science,2004,306(5693):120-124.
16.	Kimura A,Terao M,Kato A,et al.Upregulation of N-acetylglucosaminyltransferase-V by heparin-binding EGF-like growth factor induces keratinocyte proliferation and epidermal hyperplasia[J].Exp Dermatol,2012,21(7):515-519.
17.	Miyoshi E, Ihara Y, Nishikawa A, et al. Gene expression of N-acetylglucosaminyltransferases Ⅲ andⅤ:a possible implication for liver regeneration[J]. Hepatology, 1995, 22(6):1847-1855.
18.	Umaña P, Jean-Mairet J, Bailey JE. Tetracycline-regulated overexpression of glycosyltransferases in Chinese hamster ovary cells[J]. Biotechnol Bioeng, 1999, 65(5):542-549.
19.	Taniguchi N, Korekane H. Branched N-glycans and their implications for cell adhesion, signaling and clinical applications for cancer biomarkers and in therapeutics[J]. BMB Rep, 2011, 44(12):772-781.
20.	Nakahara S, Saito T, Kondo N, et al. A secreted type of beta1,6 N-acetylglucosaminyltransferaseⅤ(GnT-Ⅴ), a novel angiogenesis inducer, is regulated by gamma-secretase[J]. FASEB J, 2006, 20(14):2451-2459.
21.	Gu J, Nishikawa A, Tsuruoka N, et al. Purification and characterization of UDP-N-acetylglucosamine:alpha-6-D-mannoside beta 1-6N-acetylglucosaminyltransferase (N-acetylglucosaminyltransferase Ⅴ) from a human lung cancer cell line[J]. J Biochem, 1993, 113(5):614-619.
22.	Saito T, Miyoshi E, Sasai K, et al. A secreted type of beta 1,6-N-acetylglucosaminyltransferaseⅤ(GnT-Ⅴ) induces tumor angiogenesis without mediation of glycosylation:a novel function of GnT-Ⅴdistinct from the original glycosyltransferase activity[J]. J Biol Chem, 2002, 277(19):17002-17008.
23.	Barillari G, Albonici L, Franzese O, et al. The basic residues of placenta growth factor type 2 retrieve sequestered angiogenic factors into a soluble form:implications for tumor angiogenesis[J]. Am J Pathol, 1998, 152(5):1161-1166.
24.	Murata K, Miyoshi E, Kameyama M, et al. Expression of N-acetylglucosaminyltransferaseⅤ in colorectal cancer correlates with metastasis and poor prognosis[J]. Clin Cancer Res, 2000, 6(5):1772-1777.
25.	Handerson T, Camp R, Harigopal M, et al. Beta1,6-branched oligosaccharides are increased in lymph node metastases and predict poor outcome in breast carcinoma[J]. Clin Cancer Res, 2005, 11(8):2969-2973.
26.	Yanagi M, Aoyagi Y, Suda T, et al. N-Acetylglucosaminyltransferase Ⅴ as a possible aid for the evaluation of tumor invasiveness in patients with hepatocellular carcinoma[J]. J Gastroenterol Hepatol, 2001, 16(11):1282-1289.
27.	Hart GW, Copeland RJ. Glycomics hits the big time[J]. Cell, 2010, 143(5):672-676.
28.	Dennis JW, Granovsky M, Warren CE. Protein glycosylation in development and disease[J]. Bioessays, 1999, 21(5):412-421.
29.	Ishimura H, Takahashi T, Nakagawa H, et al. N-acetylglucosaminyltransferase Ⅴ and beta1-6 branching N-linked oligosaccharides are associated with good prognosis of patients with bladder cancer[J]. Clin Cancer Res, 2006, 12(8):2506-2511.
30.	Dosaka-Akita H, Miyoshi E, Suzuki O, et al. Expression of N-acetylglucosaminyltransferase Ⅴ is associated with prognosis and histology in non-small cell lung cancers[J]. Clin Cancer Res, 2004, 10(5):1773-1779.
31.	Takahashi T, Hagisawa S, Yoshikawa K, et al. Predictive value of N-acetylglucosaminyltransferase-Ⅴ for superficial bladder cancer recurrence[J]. J Urol, 2006, 175(1):90-93.

1. Dennis JW,Laferté S,Waghorne C,et al.Beta 1-6 branching of Asn-linked oligosaccharides is directly associated with metastasis[J].Science,1987,236(481):582-585.
2. Granovsky M,Fata J,Pawling J,et al.Suppression of tumor growth and metastasis in Mgat5-deficient mice[J].Nat Med,2000,6(3):306-312.
3. Saito H,Gu J,Nishikawa A,et al.Organization of the human N-acetylglucosaminyltransferase Ⅴgene[J].Eur J Biochem,1995,233(1):18-26.
4. Ko JH,Miyoshi E,Noda K,et al.Regulation of the GnT-Ⅴpromoter by transcription factor Ets-1 in various cancer cell lines[J].J Biol Chem,1999,274(33):22941-22948.
5. Buckhaults P,Chen L,Fregien N,et al.Transcriptional regulation of N-acetylglucosaminyltransferaseⅤ by the src oncogene[J].J Biol Chem,1997,272(31):19575-19581.
6. Chen L,Zhang W,Fregien N,et al.The her-2/neu oncogene stimulates the transcription of N-acetylglucosaminyltransferase Ⅴ and expression of its cell surface oligosaccharide products[J].Oncogene,1998,17(16):2087-2093.
7. Taniguchi N,Miyoshi E,Ko JH,et al.Implication of N-acetylglucosaminyltransferases Ⅲ andⅤ in cancer:gene regulation and signaling mechanism[J].Biochim Biophys Acta,1999,1455(2/3):287-300.
8. Lüthy R,Mclachlan AD,Eisenberg D.Secondary structure-based profiles:use of structure-conserving scoring tables in searching protein sequence databases for structural similarities[J].Proteins,1991,10(3):229-239.
9. Ihara S,Miyoshi E,Nakahara S,et al.Addition of beta1-6 GlcNAc branching to the oligosaccharide attached to Asn 772 in the serine protease domain of matriptase plays a pivotal role in its stability and resistance against trypsin[J].Glycobiology,2004,14(2):139-146.
10. Ihara S,Miyoshi E,Ko JH,et al.Prometastatic effect of N-acetylglucosaminyltransferaseⅤ is due to modification and stabilization of active matriptase by adding beta 1-6 GlcNAc branching[J].J Biol Chem,2002,277(19):16960-16967.
11. Xu Q,Isaji T,Lu Y,et al.Roles of N-acetylglucosaminyltransferase Ⅲ in epithelial-to-mesenchymal transition induced by transforming growth factor β1 (TGF-β1) in epithelial cell lines[J].J Biol Chem,2012,287(20):16563-16574.
12. Pinho SS,Figueiredo J,Cabral J,et al.E-cadherin and adherens-junctions stability in gastric carcinoma:functional implications of glycosyltransferases involving N-glycan branching biosynthesis,N-acetylglucosaminyltransferases Ⅲ andⅤ[J].Biochim Biophys Acta,2013,1830(3):2690-2700.
13. Valastyan S,Weinberg RA.Tumor metastasis:molecular insights and evolving paradigms[J].Cell,2011,147(2):275-292.
14. Gheldof A,Berx G.Cadherins and epithelial-to-mesenchymal transition[J].Prog Mol Biol Transl Sci,2013,116:317-336.
15. Partridge EA,Le Roy C,Di Guglielmo GM,et al.Regulation of cytokine receptors by Golgi N-glycan processing and endocytosis[J].Science,2004,306(5693):120-124.
16. Kimura A,Terao M,Kato A,et al.Upregulation of N-acetylglucosaminyltransferase-V by heparin-binding EGF-like growth factor induces keratinocyte proliferation and epidermal hyperplasia[J].Exp Dermatol,2012,21(7):515-519.
17. Miyoshi E, Ihara Y, Nishikawa A, et al. Gene expression of N-acetylglucosaminyltransferases Ⅲ andⅤ:a possible implication for liver regeneration[J]. Hepatology, 1995, 22(6):1847-1855.
18. Umaña P, Jean-Mairet J, Bailey JE. Tetracycline-regulated overexpression of glycosyltransferases in Chinese hamster ovary cells[J]. Biotechnol Bioeng, 1999, 65(5):542-549.
19. Taniguchi N, Korekane H. Branched N-glycans and their implications for cell adhesion, signaling and clinical applications for cancer biomarkers and in therapeutics[J]. BMB Rep, 2011, 44(12):772-781.
20. Nakahara S, Saito T, Kondo N, et al. A secreted type of beta1,6 N-acetylglucosaminyltransferaseⅤ(GnT-Ⅴ), a novel angiogenesis inducer, is regulated by gamma-secretase[J]. FASEB J, 2006, 20(14):2451-2459.
21. Gu J, Nishikawa A, Tsuruoka N, et al. Purification and characterization of UDP-N-acetylglucosamine:alpha-6-D-mannoside beta 1-6N-acetylglucosaminyltransferase (N-acetylglucosaminyltransferase Ⅴ) from a human lung cancer cell line[J]. J Biochem, 1993, 113(5):614-619.
22. Saito T, Miyoshi E, Sasai K, et al. A secreted type of beta 1,6-N-acetylglucosaminyltransferaseⅤ(GnT-Ⅴ) induces tumor angiogenesis without mediation of glycosylation:a novel function of GnT-Ⅴdistinct from the original glycosyltransferase activity[J]. J Biol Chem, 2002, 277(19):17002-17008.
23. Barillari G, Albonici L, Franzese O, et al. The basic residues of placenta growth factor type 2 retrieve sequestered angiogenic factors into a soluble form:implications for tumor angiogenesis[J]. Am J Pathol, 1998, 152(5):1161-1166.
24. Murata K, Miyoshi E, Kameyama M, et al. Expression of N-acetylglucosaminyltransferaseⅤ in colorectal cancer correlates with metastasis and poor prognosis[J]. Clin Cancer Res, 2000, 6(5):1772-1777.
25. Handerson T, Camp R, Harigopal M, et al. Beta1,6-branched oligosaccharides are increased in lymph node metastases and predict poor outcome in breast carcinoma[J]. Clin Cancer Res, 2005, 11(8):2969-2973.
26. Yanagi M, Aoyagi Y, Suda T, et al. N-Acetylglucosaminyltransferase Ⅴ as a possible aid for the evaluation of tumor invasiveness in patients with hepatocellular carcinoma[J]. J Gastroenterol Hepatol, 2001, 16(11):1282-1289.
27. Hart GW, Copeland RJ. Glycomics hits the big time[J]. Cell, 2010, 143(5):672-676.
28. Dennis JW, Granovsky M, Warren CE. Protein glycosylation in development and disease[J]. Bioessays, 1999, 21(5):412-421.
29. Ishimura H, Takahashi T, Nakagawa H, et al. N-acetylglucosaminyltransferase Ⅴ and beta1-6 branching N-linked oligosaccharides are associated with good prognosis of patients with bladder cancer[J]. Clin Cancer Res, 2006, 12(8):2506-2511.
30. Dosaka-Akita H, Miyoshi E, Suzuki O, et al. Expression of N-acetylglucosaminyltransferase Ⅴ is associated with prognosis and histology in non-small cell lung cancers[J]. Clin Cancer Res, 2004, 10(5):1773-1779.
31. Takahashi T, Hagisawa S, Yoshikawa K, et al. Predictive value of N-acetylglucosaminyltransferase-Ⅴ for superficial bladder cancer recurrence[J]. J Urol, 2006, 175(1):90-93.

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West China Medical Journal

N-乙酰氨基葡萄糖转移酶-Ⅴ与恶性肿瘤增殖和侵袭转移的关系

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