Clinical Value of DNA Ploidy Analysis in the Diagnosis of Benignancy and Malignancy with Bronchial Brushing or Bronchoalveolar Lavage Fluid_West China Medical Journal

Authors：

 MAOZhi-yuan ¹ , XIONGMei ² , RENLi ¹ , LIWei ³ , LIDe-chang ¹ , YUEYing ¹ , ZHENGJi-chun ¹

1. Department of Pathology, Air Force General Hospital of PLA, Beijing, 100142, P. R. China;
2. Department of Blood Transfusion, Air Force General Hospital of PLA, Beijing, 100142, P. R. China;
3. Canada/China Cancer Center, Tongji Medical College, Wuhan, Hubei 430022, P. R. China;

Corresponding author：

Keywords：

Bronchial brushing; Bronchoalveolar lavage fluid; DNA ploidy analysis; Cytological investigation

DOI：

10.7507/1002-0179.20140673

Video：

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Objective To investigate the clinical value of DNA ploidy analysis system in the diagnosis of benignancy or malignancy with bronchial brushing or bronchoalveolar lavage fluid. Methods We studied 96 bronchial brush tablets or bronchoalveolar lavage fluid specimens confirmed pathologically between June 2012 and June 2013, in which there were 49 cases of benignancy and 47 malignancies. Bronchial brush pieces were acquired by clinicians when they performed bronchoscopy for the patients. Each bronchoalveolar lavage fluid specimen was made into two slides, of which one was stained by HE method for cytology analysis, and the other was stained with Feulgen method for DNA ploidy analysis through automatic imaging cytometer. Results The specificity, sensitivity and accuracy of the routine cytological investigation were respectively 85.7%, 78.7% and 77.1%, while the three indexes for DNA ploidy analysis were 100.0%, 91.5% and 95.8%, respectively. Conclusion DNA ploidy analysis can improve the bronchial brushing or bronchoalveolar lavage fluid positive rate, and compared with cytological investigation, it is more specific and more sensitive with a high clinical value.

Citation： MAOZhi-yuan, XIONGMei, RENLi, LIWei, LIDe-chang, YUEYing, ZHENGJi-chun. Clinical Value of DNA Ploidy Analysis in the Diagnosis of Benignancy and Malignancy with Bronchial Brushing or Bronchoalveolar Lavage Fluid. West China Medical Journal, 2014, 29(12): 2226-2229. doi: 10.7507/1002-0179.20140673 Copy

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17.	蔡元菊, 黄文波, 马占忠. 卵巢肿瘤细胞DNA倍体和S期细胞比率的检测及临床意义[J]. 中华临床医师杂志, 2013, 7(7):3196-3197.
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19.	宋晓兵. HPV感染联合细胞DNA检测在宫颈癌筛查中的临床应用价值[J]. 中国实用医药, 2014, 9(7):90-91.

1. 荣福, 萧淑华, 刘静, 等. 常规经支气管镜针吸活检与超声引导下经支气管镜针吸活检对纵隔病变诊断的比较[J]. 中华结核和呼吸杂志, 2011, 34(2):120-122.
2. 王其生. 临床肿瘤细胞学检查[M]. 合肥:安徽科技出版社, 2005:51-71.
3. 操凤, 张焜和. 浆膜腔积液的分子生物学诊断[J]. 国外医学·肿瘤学分册, 2005, 32(3):199-201.
4. Auer G, Caspersson T, Wallgern A. DNA content and survical in mammary carcinoma[J]. Anat Quant Cytol, 1980, 2(3):161-165.
5. 张春来. 支气管镜下肺活检并刷检和经皮肺穿刺对中央型肺癌的诊断价值[J]. 临床合理用药杂志, 2011, 4(17):73-75.
6. 陶敏, 李建勇, 吴洁, 等. 支气管冲洗和肺泡灌洗液细胞DNA含量分析对肺癌的诊断价值[J]. 中华结核和呼吸杂志, 1996, 19(4):31-34.
7. 翟志敏, 戴海明, 张明黎, 等. DNA含量测定对恶性腹水的诊断价值[J]. 癌症, 2002, 21(1):105-106.
8. Osterheld MC, Liette C, Anca M. Image cytometry:an aid for cytological diagnosis of pleural effusions[J]. Diagn Cytopathol,2005, 32(3):173-176.
9. 孙小蓉, 汪键, Boecking A. DNA倍体分析系统用于宫颈癌及上皮内瘤变的诊断及预测[J]. 中华病理学杂志, 2005, 34(7):435-437.
10. Liu J, Katz R, Shin HJ, et al. Use of mailed urine specimens in diagnosing urothelial carcinoma by cytology and DNA image analysis[J]. Acta Cytol, 2005, 49(2):157-162.
11. 王丽萍, 马俊, 梁若玲, 等. 食管黏膜脱落细胞DNA倍体检测在食管癌诊断中的价值[J]. 广东医学, 2013, 36(16):2471-2474.
12. 何敏, 邝军, 朱建勇, 等. 痰细胞学联合支气管镜DNA倍体检测对肺癌的诊断意义[J]. 临床肺科杂志, 2014, 19(4):710-712.
13. 郭超楠, 任力, 李炜, 等. DNA倍体分析系统在脱落细胞学及术中病理诊断中的应用[J]. 中国妇幼保健, 2013, 28(5):848-851.
14. 李德昌, 岳颖, 郑吉春, 等. 组织印片DNA倍体分析在胃癌诊断和预后评估中的应用[J]. 空军医学杂志, 2014, 30(1):48-50.
15. 熊贵忠, 牛晓泉, 孙小蓉. 用DNA倍体分析评估口腔黏膜疾病严重程度[J]. 临床口腔医学杂志, 2013, 29(6):355-357.
16. 戴一菲, 张平安. 细针穿刺细胞学检查结合DNA倍体分析在乳腺肿块诊断中的应用[J]. 中华临床医师杂志, 2013, 12(7):5587-5589.
17. 蔡元菊, 黄文波, 马占忠. 卵巢肿瘤细胞DNA倍体和S期细胞比率的检测及临床意义[J]. 中华临床医师杂志, 2013, 7(7):3196-3197.
18. 刑应如, 胡万发, 杨路宝. DNA倍体联合肿瘤标志物检测在胸腔积液诊断中的应用[J]. 中国肿瘤外科杂志, 2013, 5(3):151-155.
19. 宋晓兵. HPV感染联合细胞DNA检测在宫颈癌筛查中的临床应用价值[J]. 中国实用医药, 2014, 9(7):90-91.

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West China Medical Journal

Clinical Value of DNA Ploidy Analysis in the Diagnosis of Benignancy and Malignancy with Bronchial Brushing or Bronchoalveolar Lavage Fluid

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