Experimental Study of the Influence of the Validity Period of Glassware Packaging on Sterilizing Instrument inside the Bio-safety Cabinet_West China Medical Journal

Authors：

 LIUDian-zhong ^1,2 , JIANGJun-qiang ² , GUOShi-li ² , LIUYao ² ,  ZHENGLi-ge ²

1. Department of Clinical Laboratory, Affiliated Hospital of Stomatology;
2. Orofacial Reconstruction and Regeneration Laboratory;Luzhou Medical College, Luzhou, Sichuan 646000, P. R. China;

Corresponding author：

ZHENGLi-ge, Email: 541148817@qq.com

Keywords：

Autoclaving; Validity period; Paper-plastic packaging; Glassware packaging

DOI：

10.7507/1002-0179.20150072

Video：

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Abstract Full text Figures/Tables Video References Cited by

Objective To explore the validity period of oral sterilizing instruments in different packages inside the bio-safety cabinet. Methods Inside the bio-safety cabinet, we brought the autoclaved dental gadgets packed in glassware and paper-plastic into microbial sampling cultivation experiment, and made observation of the bacteria growth at the 4th, 8th, 24th hour after sterilizing, with the sampling of once per 24 hours, lasting 10 days. Samples taken from the gadgets in paper-plastic each time when samples were taken from the gadgets in glassware were as the control. Results Under the condition that the bio-safety cabinet was not contaminated, no colony grew in neither group in 144 hours; 168 hours later, the cultivation results indicated that colonies started to grow（P<0.05）. Conclusion Under the condition that the bio-safety cabinet is pollution-free, the validity period of gadgets in glassware packaging for dental clinic practices is far longer than the 4-hour limit after the sterilized packages are opened, as is stipulated in the "Operating Specifications for Sterilization Technology on Dental Instruments of Medical Institutions".

Citation： LIUDian-zhong, JIANGJun-qiang, GUOShi-li, LIUYao, ZHENGLi-ge. Experimental Study of the Influence of the Validity Period of Glassware Packaging on Sterilizing Instrument inside the Bio-safety Cabinet. West China Medical Journal, 2015, 30(2): 233-235. doi: 10.7507/1002-0179.20150072 Copy

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14.	张昀, 张卫平, 吴颖, 等. 口腔诊疗器械消毒灭菌现状的思考[J]. 甘肃医药, 2010, 29(5):580-582.
15.	中国国家标准化管理委员会管理委员会. GB 15982-2012医院消毒卫生标准[S]. 2012.

1. 邹咏梅, 汪鸿文, 汪志强, 等. 杭州市口腔诊疗机构消毒灭菌现状[J]. 中国感染控制杂志, 2009, 8(3):192-194.
2. 王颖. 口腔专科医院小器械的消毒灭菌[J]. 中国消毒学杂志, 2008, 25(2):220-221.
3. 中华人民共和国卫生部. 医疗机构口腔诊疗器械消毒技术操作规范[EB/OL]. (2005-03-06)[2014-01-01]. http://moh.gov.cn/yzygj/s3576/200804/1e3b43d1a3d442eb8457cddec01658c4.shtml.
4. Raju TB, Garapati S, Agrawal R, et al. Sterilizing Endodontic Files by four different sterilization methods to prevent cross-infection:an In-vitro study[J]. J Int Oral Health, 2013, 5(6):108-112.
5. Brusca MI, Mosca CO, Nastri N, et al. Long-term sterility of orthodontic-surgical appliances[J]. J Dent Res, 2003, 82(C):10.
6. 张素芹, 王琳, 韩春云. 纸塑袋包装物品高压灭菌后有效期分析[J]. 临床医学, 2010, 30(10):123-124.
7. 中华人民共和国卫生部. WS/T 367-2012医疗机构消毒技术规范[S]. 2012.
8. 中华人民共和国卫生部. WS/T 368-2012医院空气净化管理规范[S]. 2012.
9. 余树荣. 微生物学和微生物学检验[M]. 北京:人民卫生出版社, 1997:439-449.
10. 周庭银, 赵虎. 临床微生物学诊断与图解[M]. 上海:上海科学技术出版社, 2001:15-400.
11. 赵佛容. 口腔护理学[M]. 上海:复旦大学出版社, 2004:173-174.
12. 王春燕. 口腔科诊疗器械消毒灭菌存在的问题及对策[J]. 中华医院感染学杂志, 2010, 20(1):67-68.
13. Guggenheim B, Gander M, Koller MM. A comprehensive system for washing, pre-disinfecting and sterilizing of dental and surgical instruments[J]. Oral Health Prev Dent, 2004, 2(4):335-344.
14. 张昀, 张卫平, 吴颖, 等. 口腔诊疗器械消毒灭菌现状的思考[J]. 甘肃医药, 2010, 29(5):580-582.
15. 中国国家标准化管理委员会管理委员会. GB 15982-2012医院消毒卫生标准[S]. 2012.

West China Medical Journal

Experimental Study of the Influence of the Validity Period of Glassware Packaging on Sterilizing Instrument inside the Bio-safety Cabinet

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