感染性疾病实验室诊断技术及其研究进展_West China Medical Journal

Authors：

 龙海燕 ,  冯萍

四川大学华西医院感染性疾病中心（成都 610041）;

Corresponding author：

冯萍, Email: fengp_62@163.com

Keywords：

感染性疾病; 免疫学; 分子生物学; 生物传感器; 流式细胞术

DOI：

10.7507/1002-0179.20150280

Video：

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目前全球感染性疾病现状严峻，应用于感染性疾病诊断的实验室技术种类繁多，为了解感染性疾病实验室诊断技术及其最新进展，帮助临床工作者早期、快速、准确地诊断感染性疾病，降低感染性疾病暴发的危险性，及时启动感染性疾病的正确治疗等，现从病原学检测、免疫学技术、分子生物学技术、生物传感器、流式细胞术这几个方面对感染性疾病诊断技术进行综述。

Citation： 龙海燕, 冯萍. 感染性疾病实验室诊断技术及其研究进展. West China Medical Journal, 2015, 30(5): 983-986. doi: 10.7507/1002-0179.20150280 Copy

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2.	Crowley E, Bird P, Fisher K, et al. Evaluation of the VITEK 2 gram-negative (GN) microbial identification test card:collaborative study[J]. J AOAC Int, 2012, 95(3):778-785.
3.	Kang X, Li Y, Fan L, et al. Development of an ELISA-array for simultaneous detection of five encephalitis viruses[J]. Virol J, 2012, 9(1):56.
4.	秦望森. 两种检测乙肝血清学标志物方法的临床对比[J]. 中国老年学杂志, 2011, 31(14):2665-2666.
5.	Chris Maple PA, Gray J, Brown K, et al. Performance characteristics of a quantitative, standardised varicella zoster IgG time resolved fluorescence immunoassay (VZV TRFIA) for measuring antibody following natural infection[J]. J Virol Methods, 2009, 157(1):90-92.
6.	张宏, 达文平, 苗克军, 等. UPT技术在人间鼠疫疫情处理中的展望[J]. 中国地方病防治杂志, 2011, 26(2):122-123.
7.	Li X, Chen H, Wei J, et al. The evaluation of colloidal Gold immunochromatographic assay (GICA) for rapid diagnosis of influenza A disease[J]. Clin Chem Lab Med, 2011, 49(9):1533-1537.
8.	Sano T, Smith CL, Cantor CR. Immuno-PCR:very sensitive antigen detection by means of specific antibody-DNA conjugates[J]. Science, 1992, 258(579):120-122.
9.	Ding YZ, Liu YS, Zhou JH, et al. A highly sensitive detection for foot-and-mouth disease virus by Gold nanopariticle improved immuno-PCR[J]. Virol J, 2011, 8(1):148.
10.	Monjezi R, Tan SW, Tey BT, et al. Detection of hepatitis B virus core antigen by phage display mediated TaqMan real-time immuno-PCR[J]. J Virol Methods, 2013, 187(1):121-126.
11.	Engström A, Morcillo N, Imperiale B, et al. Detection of first- and second-line drug resistance in Mycobacterium tuberculosis clinical isolates by pyrosequencing[J]. J Clin Microbiol, 2012, 50(6):2026-2033.
12.	Lin SY, Rodwell TC, Victor TC, et al. Pyrosequencing for rapid detection of extensively drug-resistant Mycobacterium tuberculosis in clinical isolates and clinical specimens[J]. J Clin Microbiol, 2014, 52(2):475-482.
13.	Zheng R, Zhu C, Guo Q, et al. Pyrosequencing for rapid detection of tuberculosis resistance in clinical isolates and sputum samples from re-treatment pulmonary tuberculosis patients[J]. BMC Infect Dis, 2014, 14(1):200.
14.	Frickmann H, Hänle A, Essig A, et al. Fluorescence in situ hybridization (FISH) for rapid identification of Salmonella spp. from agar and blood culture broth:an option for the tropics?[J]. Int J Med Microbiol, 2013, 303(5):277-284.
15.	师永霞, 黄吉城, 苏锦坤, 等. 巢式PCR法在疟疾检测及虫种鉴别中的应用[J]. 中国寄生虫学与寄生虫病杂志, 2011, 29(4):263-266.
16.	Deshpande A, Pasupuleti V, Rolston DD, et al. Diagnostic accuracy of real-time polymerase chain reaction in detection of Clostridium difficile in the stool samples of patients with suspected Clostridium difficile infection:a meta-analysis[J]. Clin Infect Dis, 2011, 53(7):e81-e90.
17.	Persson S, Jensen JN, Olsen KE. Multiplex PCR method for detection of Clostridium difficile tcdA, tcdB, cdtA, and cdtB and internal in-frame deletion of tcdC[J]. J Clin Microbiol, 2011, 49(12):4299-4300.
18.	Mohammadi-Yeganeh S, Paryan M, Mirab Samiee S, et al. Molecular beacon probes-base multiplex NASBA Real-time for detection of HIV-1 and HCV[J]. Iran J Microbiol, 2012, 4(2):47-54.
19.	王立朋, 何云燕, 夏云, 等. 核酸序列依赖扩增联合分子信标对曲霉菌感染的检测效果[J]. 第三军医大学学报, 2013, 35(15):1583-1586.
20.	Wu W, Tang YW. Emerging molecular assays for detection and characterization of respiratory viruses[J]. Clin Lab Med, 2009, 29(4):673-693.
21.	Lalande V, Barrault L, Wadel S, et al. Evaluation of a loop-mediated isothermal amplification assay for diagnosis of Clostridium difficile infections[J]. J Clin Microbiol, 2011, 49(7):2714-2716.
22.	Baumeister MA, Zhang N, Beas H, et al. A sensitive branched DNA HIV-1 signal amplification viral load assay with single day turnaround[J]. PLoS One, 2012, 7(3):e33295.
23.	郭艳玲, 刘洋, 姜广路, 等. 超分支滚环扩增技术快速检测结核分枝杆菌感染的临床应用价值[J]. 临床肺科杂志, 2012, 17(5):837-839.
24.	Gadsby NJ, Hardie A, Claas EC, et al. Comparison of the luminex respiratory virus panel fast assay with in-house real-time PCR for respiratory viral infection diagnosis[J]. J Clin Microbiol, 2010, 48(6):2213-2216.
25.	Yang YC, Yu H, Xiao DW, et al. Rapid identification of Staphylococcus aureus by surface enhanced laser desorption and ionization time of flight mass spectrometry[J]. J Microbiol Methods, 2009, 77(2):202-206.
26.	Teng Y, Zhang X, Fu Y, et al. Optimized ferrocene-functionalized ZnO nanorods for signal amplification in electrochemical immunoassay of Escherichia coli[J]. Biosens Bioelectron, 2011, 26(12):4661-4666.
27.	Li Q, Cheng W, Zhang DC, et al. Rapid and sensitive strategy for salmonella detection using an InvA Gene-Based electrochemical DNA sensor[J]. Int J electrochemical sci, 2012, 7(1):844-856.
28.	付亮, 龙军, 袁小澎. 流式细胞术快速检测产超广谱β-内酰胺酶细菌的研究[J]. 广东医学, 2011, 32(4):416-418.
29.	蒋伟. 流式细胞术测定汉滩病毒滴度方法的建立[J]. 细胞与分子免疫学杂志, 2011, 27(5):579-581.

1. Morens DM, Fauci AS. Emerging infectious diseases in 2012:20 years after the institute of medicine report[D]. mBio, 2012, 3(6):1-4.
2. Crowley E, Bird P, Fisher K, et al. Evaluation of the VITEK 2 gram-negative (GN) microbial identification test card:collaborative study[J]. J AOAC Int, 2012, 95(3):778-785.
3. Kang X, Li Y, Fan L, et al. Development of an ELISA-array for simultaneous detection of five encephalitis viruses[J]. Virol J, 2012, 9(1):56.
4. 秦望森. 两种检测乙肝血清学标志物方法的临床对比[J]. 中国老年学杂志, 2011, 31(14):2665-2666.
5. Chris Maple PA, Gray J, Brown K, et al. Performance characteristics of a quantitative, standardised varicella zoster IgG time resolved fluorescence immunoassay (VZV TRFIA) for measuring antibody following natural infection[J]. J Virol Methods, 2009, 157(1):90-92.
6. 张宏, 达文平, 苗克军, 等. UPT技术在人间鼠疫疫情处理中的展望[J]. 中国地方病防治杂志, 2011, 26(2):122-123.
7. Li X, Chen H, Wei J, et al. The evaluation of colloidal Gold immunochromatographic assay (GICA) for rapid diagnosis of influenza A disease[J]. Clin Chem Lab Med, 2011, 49(9):1533-1537.
8. Sano T, Smith CL, Cantor CR. Immuno-PCR:very sensitive antigen detection by means of specific antibody-DNA conjugates[J]. Science, 1992, 258(579):120-122.
9. Ding YZ, Liu YS, Zhou JH, et al. A highly sensitive detection for foot-and-mouth disease virus by Gold nanopariticle improved immuno-PCR[J]. Virol J, 2011, 8(1):148.
10. Monjezi R, Tan SW, Tey BT, et al. Detection of hepatitis B virus core antigen by phage display mediated TaqMan real-time immuno-PCR[J]. J Virol Methods, 2013, 187(1):121-126.
11. Engström A, Morcillo N, Imperiale B, et al. Detection of first- and second-line drug resistance in Mycobacterium tuberculosis clinical isolates by pyrosequencing[J]. J Clin Microbiol, 2012, 50(6):2026-2033.
12. Lin SY, Rodwell TC, Victor TC, et al. Pyrosequencing for rapid detection of extensively drug-resistant Mycobacterium tuberculosis in clinical isolates and clinical specimens[J]. J Clin Microbiol, 2014, 52(2):475-482.
13. Zheng R, Zhu C, Guo Q, et al. Pyrosequencing for rapid detection of tuberculosis resistance in clinical isolates and sputum samples from re-treatment pulmonary tuberculosis patients[J]. BMC Infect Dis, 2014, 14(1):200.
14. Frickmann H, Hänle A, Essig A, et al. Fluorescence in situ hybridization (FISH) for rapid identification of Salmonella spp. from agar and blood culture broth:an option for the tropics?[J]. Int J Med Microbiol, 2013, 303(5):277-284.
15. 师永霞, 黄吉城, 苏锦坤, 等. 巢式PCR法在疟疾检测及虫种鉴别中的应用[J]. 中国寄生虫学与寄生虫病杂志, 2011, 29(4):263-266.
16. Deshpande A, Pasupuleti V, Rolston DD, et al. Diagnostic accuracy of real-time polymerase chain reaction in detection of Clostridium difficile in the stool samples of patients with suspected Clostridium difficile infection:a meta-analysis[J]. Clin Infect Dis, 2011, 53(7):e81-e90.
17. Persson S, Jensen JN, Olsen KE. Multiplex PCR method for detection of Clostridium difficile tcdA, tcdB, cdtA, and cdtB and internal in-frame deletion of tcdC[J]. J Clin Microbiol, 2011, 49(12):4299-4300.
18. Mohammadi-Yeganeh S, Paryan M, Mirab Samiee S, et al. Molecular beacon probes-base multiplex NASBA Real-time for detection of HIV-1 and HCV[J]. Iran J Microbiol, 2012, 4(2):47-54.
19. 王立朋, 何云燕, 夏云, 等. 核酸序列依赖扩增联合分子信标对曲霉菌感染的检测效果[J]. 第三军医大学学报, 2013, 35(15):1583-1586.
20. Wu W, Tang YW. Emerging molecular assays for detection and characterization of respiratory viruses[J]. Clin Lab Med, 2009, 29(4):673-693.
21. Lalande V, Barrault L, Wadel S, et al. Evaluation of a loop-mediated isothermal amplification assay for diagnosis of Clostridium difficile infections[J]. J Clin Microbiol, 2011, 49(7):2714-2716.
22. Baumeister MA, Zhang N, Beas H, et al. A sensitive branched DNA HIV-1 signal amplification viral load assay with single day turnaround[J]. PLoS One, 2012, 7(3):e33295.
23. 郭艳玲, 刘洋, 姜广路, 等. 超分支滚环扩增技术快速检测结核分枝杆菌感染的临床应用价值[J]. 临床肺科杂志, 2012, 17(5):837-839.
24. Gadsby NJ, Hardie A, Claas EC, et al. Comparison of the luminex respiratory virus panel fast assay with in-house real-time PCR for respiratory viral infection diagnosis[J]. J Clin Microbiol, 2010, 48(6):2213-2216.
25. Yang YC, Yu H, Xiao DW, et al. Rapid identification of Staphylococcus aureus by surface enhanced laser desorption and ionization time of flight mass spectrometry[J]. J Microbiol Methods, 2009, 77(2):202-206.
26. Teng Y, Zhang X, Fu Y, et al. Optimized ferrocene-functionalized ZnO nanorods for signal amplification in electrochemical immunoassay of Escherichia coli[J]. Biosens Bioelectron, 2011, 26(12):4661-4666.
27. Li Q, Cheng W, Zhang DC, et al. Rapid and sensitive strategy for salmonella detection using an InvA Gene-Based electrochemical DNA sensor[J]. Int J electrochemical sci, 2012, 7(1):844-856.
28. 付亮, 龙军, 袁小澎. 流式细胞术快速检测产超广谱β-内酰胺酶细菌的研究[J]. 广东医学, 2011, 32(4):416-418.
29. 蒋伟. 流式细胞术测定汉滩病毒滴度方法的建立[J]. 细胞与分子免疫学杂志, 2011, 27(5):579-581.

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