The Expression and Clinical Significance of Livin and Caspase-3 in Patients with Middle Ear Cholesteatoma_West China Medical Journal

Authors：

 LIShu-hua , DONGChuan , WANGJie

Department of Otorhinolaryngology, Mianyang Central Hospital, Mianyang, Sichuan 621000, P. R. China;

Corresponding author：

Keywords：

Livin; Caspase-3; Immunohistochemistry; Middle ear cholesteatoma

DOI：

10.7507/1002-0179.20150311

Video：

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Objective To study the expression of inhibitor of apoptosis proteins (Livin) and aspartate-specific cysteine protease-3 (Caspase-3) in patients with middle ear cholesteatoma and its clinical significance. Method We selected 51 patients with cholesteatoma of the middle ear treated between April 2013 and March 2014 in our department to be our study subjects. Streptaridin-perosidase immunohistochemical method was adopted to detect the expression of Livin and Caspase-3 in the middle ear cholesteatoma epithelium and normal skin of external acoustic meatus. SPSS 17.0 software package was used for statistical analysis. Results The expression of Livin in cholesteatoma epithelium was significantly higher than that in the normal skin tissue of the external auditory canal (P<0.05), and the expression of Caspase-3 in cholesteatoma epithelium was significantly higher than the normal skin tissue in the external auditory canal (P<0.05). The expression of Livin and Caspase-3 in cholesteatoma epithelium was positively correlated (r=0.49, P<0.05). Conclusions There is a balance between apoptosis and inhibition of apoptosis in normal tissues, and when there is abnormal expression of Livin and Caspase-3 in normal tissues, it will cause cell apoptosis and apoptosis-inhibitory balance disorders, which causes middle ear cholesteatoma.

Citation： LIShu-hua, DONGChuan, WANGJie. The Expression and Clinical Significance of Livin and Caspase-3 in Patients with Middle Ear Cholesteatoma. West China Medical Journal, 2015, 30(6): 1083-1086. doi: 10.7507/1002-0179.20150311 Copy

1.	Fujimura M, Watanabe M, Shimizu H, et al. Expression of matrix metalloproteinases (MMPs) and tissue inhibitor of metalloproteinase (TIMP) in cerebral cavernous malformations:immunohistochemical analysis of MMP-2, -9 and TIMP-2[J]. Acta Neurochir (Wien), 2007, 149(2):179-183; discussion 183.
2.	田勇泉. 耳鼻咽喉头颈外科学[M]. 8版. 北京:人民卫生出版社, 2013:329.
3.	Ergün S, Carlsöö B, Zheng X. Apoptosis in meatal skin, cholesteatoma and squamous cell carcinoma of the ear[J]. Clin Otolaryngol Allied Sci, 1999, 24(4):280-285.
4.	潘宏光, 崔永华, 高起学. 中耳胆脂瘤分子生物学研究的新进展[J]. 临床耳鼻咽喉科杂志, 2000, 14(5):235-237.
5.	Albino AP, Reed JA, Bogdany JK, et al. Expression of p53 protein in human middle ear cholesteatomas:pathogenetic implications[J]. Am J Otol, 1998, 19(1):30-36.
6.	Kojima H, Miyazaki H, Tanaka Y, et al. Role of Bcl-xL protein in differentiation and apoptosis of human middle ear cholesteatoma epithelium[J]. Arch Otolaryngol Head Neck Surg, 1999, 125(7): 738-742.
7.	李冬, 黄芳, 龚树生, 等. 凋亡抑制蛋白Survivin及Bcl-2在中耳胆脂瘤上皮中的表达及意义[J]. 中国耳鼻咽喉颅底外科杂志, 2006, 12(5):334-338.
8.	Olszewska E, Chodynicki S, Chyczewski L. Apoptosis in the pathogenesis of cholesteatoma in adults[J]. Eur Arch Otorhinolaryngol, 2006, 263(5):409-413.
9.	关芳灵, 马敏, 尚丽芳, 等. X连锁凋亡抑制蛋白在胆脂瘤中的表达及其与细胞凋亡的关系[J]. 山西医药杂志, 2008, 37(6):492-494.
10.	Lin SH, Deng G, Huang Q, et al. KIAP, a novel member of the inhibitor of apoptosis protein family[J]. Biochem Biophys Res Commun, 2000, 279(3):820-831.
11.	Vucic D, Stennicke HR, Pisabarro MT, et al. ML-IAP, a novel inhibitor of apoptosis that is preferentially expressed in human melanomas[J]. Curr Biol, 2000, 10(21):1359-1366.
12.	Gazzaniga P, Gradilone A, Giuliani L, et al. Expression and prognostic significance of LIVIN, SURVIVIN and other apoptosis-related genes in the progression of superficial bladder cancer[J]. Ann Oncol, 2003, 14(1):85-90.
13.	彭利, 刘立中. 中耳胆脂瘤上皮中Livin、p73的表达与其发生关系研究[J]. 中华耳科学杂志, 2010, 8(3):315-318.
14.	杨宁, 蒋立新. 凋亡抑制蛋白Livin及Survivin在中耳胆脂瘤上皮的表达及意义[J]. 听力学及言语疾病杂志, 2011, 19(4):356-358.
15.	伍千元, 李建军, 李龙平. Survivin及Livin在中耳胆脂瘤组织中的表达及相关性研究[J]. 中国实用医药, 2011, 6(1):3-4.
16.	郝艳, 李珂, 王建亭, 等. NF-κBP65和caspase-3在中耳胆脂瘤中的表达及其临床意义[J]. 山西医科大学学报, 2011, 42(4):288-291.
17.	潘松林, 龚树生, 贺红斌. 细胞外凋亡途径与中耳胆脂瘤细胞凋亡[J]. 中国耳鼻咽喉头颈外科, 2007, 14(12):699-701.
18.	Bao GM, Zhang CW, Wang GX. Expressions of livin and IGF-Ⅱ in colorectalcarcinoma and their relationship[J]. Chin Germ J Clin Oncol, 2010, 9(8):470-474.
19.	Park HR, Min SK, Min K, et al. Increased expression of p63 and survivin in cholesteatomas[J]. Acta Otolaryngol, 2009, 129(3): 268-272.

1. Fujimura M, Watanabe M, Shimizu H, et al. Expression of matrix metalloproteinases (MMPs) and tissue inhibitor of metalloproteinase (TIMP) in cerebral cavernous malformations:immunohistochemical analysis of MMP-2, -9 and TIMP-2[J]. Acta Neurochir (Wien), 2007, 149(2):179-183; discussion 183.
2. 田勇泉. 耳鼻咽喉头颈外科学[M]. 8版. 北京:人民卫生出版社, 2013:329.
3. Ergün S, Carlsöö B, Zheng X. Apoptosis in meatal skin, cholesteatoma and squamous cell carcinoma of the ear[J]. Clin Otolaryngol Allied Sci, 1999, 24(4):280-285.
4. 潘宏光, 崔永华, 高起学. 中耳胆脂瘤分子生物学研究的新进展[J]. 临床耳鼻咽喉科杂志, 2000, 14(5):235-237.
5. Albino AP, Reed JA, Bogdany JK, et al. Expression of p53 protein in human middle ear cholesteatomas:pathogenetic implications[J]. Am J Otol, 1998, 19(1):30-36.
6. Kojima H, Miyazaki H, Tanaka Y, et al. Role of Bcl-xL protein in differentiation and apoptosis of human middle ear cholesteatoma epithelium[J]. Arch Otolaryngol Head Neck Surg, 1999, 125(7): 738-742.
7. 李冬, 黄芳, 龚树生, 等. 凋亡抑制蛋白Survivin及Bcl-2在中耳胆脂瘤上皮中的表达及意义[J]. 中国耳鼻咽喉颅底外科杂志, 2006, 12(5):334-338.
8. Olszewska E, Chodynicki S, Chyczewski L. Apoptosis in the pathogenesis of cholesteatoma in adults[J]. Eur Arch Otorhinolaryngol, 2006, 263(5):409-413.
9. 关芳灵, 马敏, 尚丽芳, 等. X连锁凋亡抑制蛋白在胆脂瘤中的表达及其与细胞凋亡的关系[J]. 山西医药杂志, 2008, 37(6):492-494.
10. Lin SH, Deng G, Huang Q, et al. KIAP, a novel member of the inhibitor of apoptosis protein family[J]. Biochem Biophys Res Commun, 2000, 279(3):820-831.
11. Vucic D, Stennicke HR, Pisabarro MT, et al. ML-IAP, a novel inhibitor of apoptosis that is preferentially expressed in human melanomas[J]. Curr Biol, 2000, 10(21):1359-1366.
12. Gazzaniga P, Gradilone A, Giuliani L, et al. Expression and prognostic significance of LIVIN, SURVIVIN and other apoptosis-related genes in the progression of superficial bladder cancer[J]. Ann Oncol, 2003, 14(1):85-90.
13. 彭利, 刘立中. 中耳胆脂瘤上皮中Livin、p73的表达与其发生关系研究[J]. 中华耳科学杂志, 2010, 8(3):315-318.
14. 杨宁, 蒋立新. 凋亡抑制蛋白Livin及Survivin在中耳胆脂瘤上皮的表达及意义[J]. 听力学及言语疾病杂志, 2011, 19(4):356-358.
15. 伍千元, 李建军, 李龙平. Survivin及Livin在中耳胆脂瘤组织中的表达及相关性研究[J]. 中国实用医药, 2011, 6(1):3-4.
16. 郝艳, 李珂, 王建亭, 等. NF-κBP65和caspase-3在中耳胆脂瘤中的表达及其临床意义[J]. 山西医科大学学报, 2011, 42(4):288-291.
17. 潘松林, 龚树生, 贺红斌. 细胞外凋亡途径与中耳胆脂瘤细胞凋亡[J]. 中国耳鼻咽喉头颈外科, 2007, 14(12):699-701.
18. Bao GM, Zhang CW, Wang GX. Expressions of livin and IGF-Ⅱ in colorectalcarcinoma and their relationship[J]. Chin Germ J Clin Oncol, 2010, 9(8):470-474.
19. Park HR, Min SK, Min K, et al. Increased expression of p63 and survivin in cholesteatomas[J]. Acta Otolaryngol, 2009, 129(3): 268-272.

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The Expression and Clinical Significance of Livin and Caspase-3 in Patients with Middle Ear Cholesteatoma

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