Effect of water soluble chitosan on the apoptosis of peritoneal macrophage induced by lipopolysaccharides_West China Medical Journal

Authors：

 WANG Dashan

Molecular Biology Research Center, Shandong Medical College, Linyi, Shandong 276000, P. R. China;

Corresponding author：

WANG Dashan, Email: wds1055@163.com

Keywords：

Water soluble chitosan; Macrophage; Apoptosis

DOI：

10.7507/1002-0179.201504030

Video：

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Objective To study the effect of water soluble chitosan (WSC) on the apoptosis of peritoneal macrophage induced by lipopolysaccharides (LPS), and discuss the mechanism. Methods Peritoneal macrophages were divided to three groups: phosphate buffered saline (PBS) group, LPS group and LPS plus WSC group. At hour 24, apoptosis cell and active caspase-3 were detected by flow cytometry; nitric oxide (NO) was determined with Griess reagent. Results There were more apoptosis cells in the LPS group than the PBS group. The percentage of apoptosis cells was significantly decreased in the LPS plus WSC group than the LPS group. The expression of active caspase-3 and the secretion of NO were also inhibited by WSC after LPS intervention. Conclusion WSC inhibits apoptosis of peritoneal macrophage induced by LPS.

Citation： WANGDashan. Effect of water soluble chitosan on the apoptosis of peritoneal macrophage induced by lipopolysaccharides. West China Medical Journal, 2017, 32(1): 1-4. doi: 10.7507/1002-0179.201504030 Copy

1.	Younes I, Rinaudo M. Chitin and chitosan preparation from Marine sources. structure, properties and applications. Mar Drugs, 2015, 13(3): 1133-1174.
2.	Jung WJ, Park RD. Bioproduction of chitooligosaccharides: present and perspectives. Mar Drugs, 2014, 12(11): 5328-5356.
3.	Porporatto C, Bianco ID, Correa SG. Local and systemic activity of the polysaccharide chitosan at lymphoid tissues after oral administration. J Leukoc Biol, 2005, 78(1): 62-69.
4.	Han YP, Zhao LH, Yu ZJ,et al. Role of mannose receptor in oligochitosan-mediated stimulation of macrophage function. Int Immunopharmacol, 2005, 5(10): 1533-1542.
5.	Azuma K, Izumi R, Osaki T,et al. Chitin, chitosan, and its derivatives for wound healing: old and new materials. J Funct Biomater, 2015, 6(1): 104-142.
6.	Chou TC, Fu E, Shen EC. Chitosan inhibits prostaglandin E2 formation and cyclooxygenase-2 induction in lipopolysaccharide-treated RAW 264.7 macrophages. Biochem Biophys Res Commun, 2003, 308(2): 403-407.
7.	Mills CD, Thomas AC, Lenz LL,et al. Macroplage: SHIP of immunity. Front Immunol, 2014, 5: 620.
8.	Li ZH, Yang F, Yang RD. Synthesis and characterization of chitosan derivatives with dual-antibacterial functional groups. Int J Biol Macromol, 2015, 75: 378-387.
9.	Feng J, Zhao LH, Yu QQ. Receptor-mediated stimulatory effect of oligochitosan in macrophages. Biochem Biophys Res Commun, 2004, 317(2): 414-420.
10.	Mayol L, De Stefano D, Campani V,et al. Design and characterization of a chitosan physical gel promoting wound healing in mice. J Mater Sci Mater Med, 2014, 25(6): 1483-1493.
11.	Booth LA, Tavallai S, Hamed HA,et al. The role of cell signalling in the crosstalk between autophagy and apoptosis. Cell Signal, 2014, 26(3): 549-555.
12.	Tam VC, Aderem A. Macrophage activation as an effector mechanism for Cell-Mediated immunity. J Immunol, 2014, 193(7): 3183-3184.
13.	Gotoh T, Mori M. Arginase Ⅱ downregulates nitric oxide (NO) production and prevents NO-mediated apoptosis in murine macrophage-derived RAW 264.7 cells. J Cell Biol, 1999, 144(3): 427-434.
14.	Dragovich T, Rudin CM, Thompson CB. Signal transduction pathways that regulate cell survival and cell death. Oncogene, 1998, 17(25): 3207-3213.
15.	Kumar S. Caspase function in programmed cell death. Cell Death Differ, 2007, 14(1): 32-43.
16.	Chaudhari BR, Murphy RF, Agrawal DK. Following the TRAIL to apoptosis. Immunol Res, 2006, 35(3): 249-262.
17.	Cosentino K, Garcia-Saez AJ. Mitochondrial alterations in apoptosis. Chem Phys Lipids, 2014, 181: 62-75.
18.	Volkmann N, Marassi FM, Newmeyer DD,et al. The rheostat in the membrane: BCL-2 family proteins and apoptosis. Cell Death Differ, 2014, 21(2): 206-215.
19.	Sano R, Reed JC. ER stress-induced cell death mechanisms. Biochim Biophys Acta, 2013, 1833(12): 3460-3470.
20.	Kumar S. Regulation of caspase activation in apoptosis: implications in pathogenesis and treatment of disease. Clin Exp Pharmacol Physiol, 1999, 26(4): 295-303.

1. Younes I, Rinaudo M. Chitin and chitosan preparation from Marine sources. structure, properties and applications. Mar Drugs, 2015, 13(3): 1133-1174.
2. Jung WJ, Park RD. Bioproduction of chitooligosaccharides: present and perspectives. Mar Drugs, 2014, 12(11): 5328-5356.
3. Porporatto C, Bianco ID, Correa SG. Local and systemic activity of the polysaccharide chitosan at lymphoid tissues after oral administration. J Leukoc Biol, 2005, 78(1): 62-69.
4. Han YP, Zhao LH, Yu ZJ,et al. Role of mannose receptor in oligochitosan-mediated stimulation of macrophage function. Int Immunopharmacol, 2005, 5(10): 1533-1542.
5. Azuma K, Izumi R, Osaki T,et al. Chitin, chitosan, and its derivatives for wound healing: old and new materials. J Funct Biomater, 2015, 6(1): 104-142.
6. Chou TC, Fu E, Shen EC. Chitosan inhibits prostaglandin E2 formation and cyclooxygenase-2 induction in lipopolysaccharide-treated RAW 264.7 macrophages. Biochem Biophys Res Commun, 2003, 308(2): 403-407.
7. Mills CD, Thomas AC, Lenz LL,et al. Macroplage: SHIP of immunity. Front Immunol, 2014, 5: 620.
8. Li ZH, Yang F, Yang RD. Synthesis and characterization of chitosan derivatives with dual-antibacterial functional groups. Int J Biol Macromol, 2015, 75: 378-387.
9. Feng J, Zhao LH, Yu QQ. Receptor-mediated stimulatory effect of oligochitosan in macrophages. Biochem Biophys Res Commun, 2004, 317(2): 414-420.
10. Mayol L, De Stefano D, Campani V,et al. Design and characterization of a chitosan physical gel promoting wound healing in mice. J Mater Sci Mater Med, 2014, 25(6): 1483-1493.
11. Booth LA, Tavallai S, Hamed HA,et al. The role of cell signalling in the crosstalk between autophagy and apoptosis. Cell Signal, 2014, 26(3): 549-555.
12. Tam VC, Aderem A. Macrophage activation as an effector mechanism for Cell-Mediated immunity. J Immunol, 2014, 193(7): 3183-3184.
13. Gotoh T, Mori M. Arginase Ⅱ downregulates nitric oxide (NO) production and prevents NO-mediated apoptosis in murine macrophage-derived RAW 264.7 cells. J Cell Biol, 1999, 144(3): 427-434.
14. Dragovich T, Rudin CM, Thompson CB. Signal transduction pathways that regulate cell survival and cell death. Oncogene, 1998, 17(25): 3207-3213.
15. Kumar S. Caspase function in programmed cell death. Cell Death Differ, 2007, 14(1): 32-43.
16. Chaudhari BR, Murphy RF, Agrawal DK. Following the TRAIL to apoptosis. Immunol Res, 2006, 35(3): 249-262.
17. Cosentino K, Garcia-Saez AJ. Mitochondrial alterations in apoptosis. Chem Phys Lipids, 2014, 181: 62-75.
18. Volkmann N, Marassi FM, Newmeyer DD,et al. The rheostat in the membrane: BCL-2 family proteins and apoptosis. Cell Death Differ, 2014, 21(2): 206-215.
19. Sano R, Reed JC. ER stress-induced cell death mechanisms. Biochim Biophys Acta, 2013, 1833(12): 3460-3470.
20. Kumar S. Regulation of caspase activation in apoptosis: implications in pathogenesis and treatment of disease. Clin Exp Pharmacol Physiol, 1999, 26(4): 295-303.

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Effect of water soluble chitosan on the apoptosis of peritoneal macrophage induced by lipopolysaccharides

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