Emulsified Isoflurane Induces Postconditioning against Myocardial Ischemia and Reperfusion Injury in Rats_West China Medical Journal

Authors：

 KONGXiang-yun , HUZhao-yang , LIUJin ,  LUOChao-zh

Department of Anesthesiology, West China Hospital, Sichuan University, Chengdu, Sichuan 610041, P. R. China;

Corresponding author：

LUOChao-zh, Email: chaozhil@aliyun.com

Keywords：

Emulsified isoflurane; Postconditioning; Ischemia and reperfusion injury; Rats; Tumor necrosis factor

DOI：

10.7507/1002-0179.20150418

Video：

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Objective To investigate whether emulsified isoflurane applied after an ischemic episode induces postconditioning in an ischemia model of myocardial injury and its underlying mechanism. Methods Between March and October 2012, using a model of in situ myocardial ischemia and reperfusion injury in rats, cardioprotective effects of emulsified isoflurane were examined by determining infarct size, myocardial damage markers and the concentration of tumor necrosis factor (TNF)-α. Results Emulsified isoflurane postconditioning limited infarct size compared with control groups. It increased serum concentrations of superoxide dismutase while decreased malonaldehyde. TNF-α positive cells were also significantly reduced in emulsified isoflurane group compared with control group. Infusion of intralipid had no effect on infarct size or other variables. Conclusion Intravenous administration of emulsified isoflurane after reperfusion protects hearts against reperfusion injury, which may be mediated by the inhibition of cardiac damage markers and the concentration of TNF-α.

Citation： KONGXiang-yun, HUZhao-yang, LIUJin, LUOChao-zh. Emulsified Isoflurane Induces Postconditioning against Myocardial Ischemia and Reperfusion Injury in Rats. West China Medical Journal, 2015, 30(8): 1461-1464. doi: 10.7507/1002-0179.20150418 Copy

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7.	Hu ZY, Luo NF, Liu J. The protective effects of emulsified isoflurane on myocardial ischemia and reperfusion injury in rats[J]. Can J Anaesth, 2009, 56(2):115-125.
8.	Turer AT, Hiu JA. Pathogenesis of myocardial ischemia-reperfusion injury and rationale for therapy[J]. Am J Cardiol, 2010, 106(3):360-368.
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10.	Yellon DM, Downey JM. Preconditioning the myocardium:from cellular physiology to clinical cardiology[J]. Physiol Rev, 2003, 83(4):1113-1151.
11.	Chiari PC, Pagel PS, Tanaka K, et al. Intravenous emulsified halogenated anesthetics produce acute and delayed preconditioning against myocardial infarction in rabbits[J]. Anesthesiology, 2004, 101(5):1160-1166.
12.	Rao Y, Wang YL, Chen YQ, et al. Protective effects of emulsified isoflurane after myocardial ischemia reperfusion injury and its mechanism in rabbits[J]. Chin J Traumatol, 2009, 12(1):18-21.
13.	Kaminski KA, Bonda TA, Korecki J, et al. Oxidative stress and neutrophil activation——the two keystones of ischemia/reperfusion injury[J]. Int J Cardiol, 2002, 86(1):41-59.
14.	Czermak BJ, Sarma V, Bless NM, et al. In vitro and in vivo dependency of chemokine generation on C5a and TNF-alpha[J].J Immunol, 1999, 162(4):2321-2325.
15.	Shames BD, Barton HH, Reznikov LL, et al. Ischemia alone is sufficient to induce tnf-α mrna and peptide in the myocardium[J]. Shock, 2002, 17(2):114-119.

1. Braunwald E, Kloner RA. Myocardial reperfusion:a double-edged sword?[J]. J Clin Invest, 1985, 76(5):1713-1719.
2. Piper HM, Garcia-Dorado D, Ovize M. A fresh look at reperfusion injury[J]. Cardiovasc Res, 1998, 38(2):291-300.
3. Zhou JX, Luo NF, Liang XM, et al. The efficacy and safety of intravenous emulsified isoflurane in rats[J]. Anesth Analg, 2006, 102(1):129-134.
4. Yang XL, Ma HX, Yang ZB, et al. Comparison of minimum alveolar concentration between intravenous isoflurane lipid emulsion and inhaled isoflurane in dogs[J]. Anesthesiology 2006,104(3):482-487.
5. Rao Y, Wang YL, Zhang WS, et al. Emulsified isoflurane produces cardiac protection after ischemia-reperfusion injury in rabbits[J]. Anesth Analg, 2008, 106(5):1353-1359.
6. Hu ZY, Liu J. Effects of emulsified isoflurane on haemodynamics and cardiomyocyte apoptosis in rats with myocardial ischaemia[J]. Clin Exp Pharmacol Physiol, 2009, 36(8):776-783.
7. Hu ZY, Luo NF, Liu J. The protective effects of emulsified isoflurane on myocardial ischemia and reperfusion injury in rats[J]. Can J Anaesth, 2009, 56(2):115-125.
8. Turer AT, Hiu JA. Pathogenesis of myocardial ischemia-reperfusion injury and rationale for therapy[J]. Am J Cardiol, 2010, 106(3):360-368.
9. Yellon DM, Hausenloy DJ. Myocardial reperfusion injury[J]. N Engl J Med, 2007, 357(11):1121-1135.
10. Yellon DM, Downey JM. Preconditioning the myocardium:from cellular physiology to clinical cardiology[J]. Physiol Rev, 2003, 83(4):1113-1151.
11. Chiari PC, Pagel PS, Tanaka K, et al. Intravenous emulsified halogenated anesthetics produce acute and delayed preconditioning against myocardial infarction in rabbits[J]. Anesthesiology, 2004, 101(5):1160-1166.
12. Rao Y, Wang YL, Chen YQ, et al. Protective effects of emulsified isoflurane after myocardial ischemia reperfusion injury and its mechanism in rabbits[J]. Chin J Traumatol, 2009, 12(1):18-21.
13. Kaminski KA, Bonda TA, Korecki J, et al. Oxidative stress and neutrophil activation——the two keystones of ischemia/reperfusion injury[J]. Int J Cardiol, 2002, 86(1):41-59.
14. Czermak BJ, Sarma V, Bless NM, et al. In vitro and in vivo dependency of chemokine generation on C5a and TNF-alpha[J].J Immunol, 1999, 162(4):2321-2325.
15. Shames BD, Barton HH, Reznikov LL, et al. Ischemia alone is sufficient to induce tnf-α mrna and peptide in the myocardium[J]. Shock, 2002, 17(2):114-119.

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Emulsified Isoflurane Induces Postconditioning against Myocardial Ischemia and Reperfusion Injury in Rats

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