p38丝裂原活化蛋白激酶抑制剂对葡聚糖硫酸钠诱导的溃疡性结肠炎大鼠结肠传输功能的影响_West China Medical Journal

Authors：

 杨昆 , 闫静 ,  黄晓丽

四川大学华西医院老年医学中心（成都 610041）;

Corresponding author：

黄晓丽, Email: xiaoxiao334@sina.cn

Keywords：

p38丝裂原活化蛋白激酶; p38丝裂原活化蛋白激酶抑制剂; 溃疡性结肠炎; 葡聚糖硫酸钠结肠炎; 肠道动力障碍

DOI：

10.7507/1002-0179.20150431

Video：

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目的探讨p38丝裂原活化蛋白激酶（p38MAPK）抑制剂在葡聚糖硫酸钠（DSS）诱导的溃疡性结肠炎大鼠肠道动力障碍中的作用。方法 34只Sprague-Dawley大鼠用随机数字表法随机分成4组：正常对照组（正常组，n=8），DSS模型组（DSS组，n=8），DSS+生理盐水（NS）组（DSS+NS组，n=9），DSS+SB203580干预组（SB203580组，n=9）。除正常组外，所有大鼠每日饮用5% DSS溶液，SB203580组大鼠在饮用5%DSS溶液72 h以后，每天予以SB203580（1 mg/kg体质量）进行腹腔注射，观察各组大鼠的疾病活动指数（DAI），以酚红法测定大鼠结肠传输功能。结果 ①DSS组和DSS+NS组大鼠的DAI评分明显高于正常组，SB203580组DAI评分明显降低，但仍然高于正常组（P＜0.05），DSS组和DSS+NS组之间差异无统计学意义（P＞0.05）。②与正常对照组比较，DSS组和DSS+NS组大鼠的结肠传输功能明显延迟，经SB203580干预后，其结肠传输功能明显改善（P＜0.05），DSS组和DSS+NS组之间差异无统计学意义（P＞0.05）。结论 SB203580能阻断p38MAPK信号转导通路，改善DSS诱导的结肠炎症，从而改善结肠传输功能。

Citation： 杨昆, 闫静, 黄晓丽. p38丝裂原活化蛋白激酶抑制剂对葡聚糖硫酸钠诱导的溃疡性结肠炎大鼠结肠传输功能的影响. West China Medical Journal, 2015, 30(8): 1505-1507. doi: 10.7507/1002-0179.20150431 Copy

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11.	Murthy SN, Cooper HS, Shim H, et al. Treatment of dextran sulfate sodium-induced murine colitis by intracolonic cyclosporin[J]. Dig Dis Sci, 1993, 38(9):1722-1734.
12.	Izbéki F, Wittmann T, Jancsó G, et al. Inhibition of gastric emptying and small intestinal Transit by ethanol is mediated by capsaicin-sensitive afferent nerves[J]. Naunyn Schmiedebergs Arch Pharmacol, 2002, 365(1):17-21.
13.	Lelievre V, Favrais G, Abad C, et al. Gastrointestinal dysfunction in mice with a targeted mutation in the gene encoding vasoactive intestinal polypeptide:a model for the study of intestinal ileus and Hirschsprung's disease[J]. Peptides, 2007, 28(9):1688-1699.
14.	Rao SS, Read NW, Brown C, et al. Studies on the mechanism of bowel disturbance in ulcerative colitis[J]. Gastroenterology, 1987, 93(5):934-940.
15.	Mackie TT. The medical management of chronic ulcerative colitis[J]. JAMA, 1938(111):2071-2076.
16.	Jouët P, Sarna SK, Singaram C, et al. Immunocytes and abnormal gastrointestinal motor activity during ileitis in dogs[J]. Am J Physiol, 1995, 269(6 Pt 1):G913-G924.
17.	Kalff JC, Carlos TM, Schraut WH, et al. Surgically induced leukocytic infiltrates within the rat intestinal muscularis mediate postoperative ileus[J]. Gastroenterology, 1999, 117(2):378-387.
18.	Robinson MJ, Cobb MH. Mitogen-activated protein kinase pathways[J]. Curr Opin Cell Biol, 1997, 9(2):180-186.
19.	Hommes DW, Peppelenbosch MP, Van Deventer SJ. Mitogen activated protein (MAP) kinase signal transduction pathways and novel anti-inflammatory targets[J]. Gut, 2003, 52(1):144-151.
20.	Waetzig GH, Seegert D, Rosenstiel P, et al. p38 mitogen-activated protein kinase is activated and linked to TNF-alpha signaling in inflammatory bowel disease[J]. J Immunol, 2002, 168(10):5342-5351.

1. 中华医学会消化病学分会炎症性肠病组. 炎症性肠病诊断与治疗的共识意见(2012年·广州)[J]. 胃肠病学, 2012, 51(12):763-781.
2. Vrees MD, Pricolo VE, Potenti FM, et al. Abnormal motility in patients with ulcerative colitis:the role of inflammatory cytokines[J]. Arch Surg, 2002, 137(4):439-445; discussion 445-446.
3. Ozaki H, Hori M, Kinoshita K, et al. Intestinal dysmotility in inflammatory bowel disease:mechanisms of the reduced activity of smooth muscle contraction[J]. Inflammopharmacology, 2005, 13(1/2/3):103-111.
4. Bossone C, Hosseini JM, Piñeiro-Carrero V, et al. Alterations in spontaneous contractions in vitro after repeated inflammation of rat distal colon[J]. Am J Physiol Gastrointest Liver Physiol, 2001, 280(5):G949-G957.
5. Collins SM. The immunomodulation of enteric neuromuscular function:implications for motility and inflammatory disorders[J]. Gastroenterology, 1996, 111(6):1683-1699.
6. Sethi AK, Sarna SK. Colonic motor activity in acute colitis in conscious dogs[J]. Gastroenterology, 1991, 100(4):954-963.
7. Ono K, Han JH. The p38 signal transduction pathway Activation and function[J]. Cell Signal, 2000, 12(1):1-13.
8. Kyriakis JM, Avruch J. Mammalian mitogen-activated protein kinase signal transduction pathways activated by stress and inflammation[J]. Physiol Rev, 2001, 81(2):807-869.
9. Cooper HS, Murthy SN, Shah RS, et al. Clinicopathologic study of dextran sulfate sodium experimental murine colitis[J]. Lab Invest, 1993, 69(2):238-249.
10. Hollenbach E, Neumann M, Vieth M, et al. Inhibition of p38 MAP kinase-and RICK/NF-kappaB-signaling suppresses inflammatory bowel disease[J]. FASEB J, 2004, 18(13):1550-1552.
11. Murthy SN, Cooper HS, Shim H, et al. Treatment of dextran sulfate sodium-induced murine colitis by intracolonic cyclosporin[J]. Dig Dis Sci, 1993, 38(9):1722-1734.
12. Izbéki F, Wittmann T, Jancsó G, et al. Inhibition of gastric emptying and small intestinal Transit by ethanol is mediated by capsaicin-sensitive afferent nerves[J]. Naunyn Schmiedebergs Arch Pharmacol, 2002, 365(1):17-21.
13. Lelievre V, Favrais G, Abad C, et al. Gastrointestinal dysfunction in mice with a targeted mutation in the gene encoding vasoactive intestinal polypeptide:a model for the study of intestinal ileus and Hirschsprung's disease[J]. Peptides, 2007, 28(9):1688-1699.
14. Rao SS, Read NW, Brown C, et al. Studies on the mechanism of bowel disturbance in ulcerative colitis[J]. Gastroenterology, 1987, 93(5):934-940.
15. Mackie TT. The medical management of chronic ulcerative colitis[J]. JAMA, 1938(111):2071-2076.
16. Jouët P, Sarna SK, Singaram C, et al. Immunocytes and abnormal gastrointestinal motor activity during ileitis in dogs[J]. Am J Physiol, 1995, 269(6 Pt 1):G913-G924.
17. Kalff JC, Carlos TM, Schraut WH, et al. Surgically induced leukocytic infiltrates within the rat intestinal muscularis mediate postoperative ileus[J]. Gastroenterology, 1999, 117(2):378-387.
18. Robinson MJ, Cobb MH. Mitogen-activated protein kinase pathways[J]. Curr Opin Cell Biol, 1997, 9(2):180-186.
19. Hommes DW, Peppelenbosch MP, Van Deventer SJ. Mitogen activated protein (MAP) kinase signal transduction pathways and novel anti-inflammatory targets[J]. Gut, 2003, 52(1):144-151.
20. Waetzig GH, Seegert D, Rosenstiel P, et al. p38 mitogen-activated protein kinase is activated and linked to TNF-alpha signaling in inflammatory bowel disease[J]. J Immunol, 2002, 168(10):5342-5351.

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West China Medical Journal

p38丝裂原活化蛋白激酶抑制剂对葡聚糖硫酸钠诱导的溃疡性结肠炎大鼠结肠传输功能的影响

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