Comparison of Evaluation in vitro between Erhuang Gel Paste and Its Ointment_West China Medical Journal

Authors：

 ZhuLiyang ,  MaJian , TuHe , XiangMeng , WangRui

Manufacturing Laboratory, Sichuan Provincial Orthopedic Hospital, Chengdu 610041, China;

Corresponding author：

MaJian, Email: majian222@sina.com

Keywords：

Release experiment; Transdermal experiment; Gel paste; Osthole

DOI：

10.7507/1002-0179.201600196

Video：

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Abstract Full text Figures/Tables Video References Cited by

Objective To compare the in vitro release and transdermal capability between Erhuang gel paste and its ointment. Methods Strat-M membrane and advanced Franz diffusion cell were used to conduct the in vitro release and transdermal experiment. The content of osthole, the effective component of the key herb radix angelicae tuhuo in the preparations was quantitatively determined by high performance liquid chromatography (HPLC) method to determine the in vitro release rate, transdermal quantity and transdermal rate. Results The transdermal penetration formula of Erhuang gel paste was L_n=0.443 6t+1.350 9, r=0.964 4. The cumulative in vitro release rate, transdermal quantity and transdermal rate were respectively 62.90%, 0.067 2 mg/g and 20.82%. The transdermal penetration formula of Erhuang ointment was L_n=0.205 1t-0.244 7, r=0.995 6. The cumulative in vitro release rate, transdermal quantity and transdermal rate were respectively 77.64%, 0.013 1mg/g and 9.36%. Conclusion The transdermal capability of Erhuang gel paste is obviously better than its ointment, and the gel paste is more convenient to use and is unlikely to cause allergic reactions.

Citation： ZhuLiyang, MaJian, TuHe, XiangMeng, WangRui. Comparison of Evaluation in vitro between Erhuang Gel Paste and Its Ointment. West China Medical Journal, 2016, 31(4): 724-727. doi: 10.7507/1002-0179.201600196 Copy

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1. Nylén K, Ost M, Csajbok LZ, et al. Increased serum-GFAP in patients with severe traumatic brain injury is related to outcome[J]. J Neurol Sci, 2006, 240(1/2): 85-91.
2. Deng P, Xu HH, Zhu JZ. Preliminary study on using changes in astrocytes and neurons in the brain-stem of the rat after stab wounds for determining the time of injury[J]. Forensic Sci Int, 1999, 103(3): 181-191.
3. 王炜煜, 秦启生, 易继林. 大鼠脑挫伤后GFAP和iNOS表达与损伤时间关系的实验研究[J]. 中国法医学杂志, 2004, 19(5): 271-274.
4. Nieto-Sampedro M, Lewis ER, Cotman CW, et al. Brain injury causes a time-dependent increase in neuronotrophic activity at the lesion site[J]. Science, 1982, 217(4562): 860-861.
5. 杨静, 王晔, 陈晓刚, 等. 大鼠脑液压冲击伤caspase-8的表达[J]. 法医学杂志, 2006, 22(1): 1-3.
6. 陈锐, 余彬华, 胡玲, 等. 大鼠脑震荡伤后脑组织HSP70、bFGF及TGF-β1表达变化[J]. 法医学杂志, 2009, 25(4): 249-253.
7. 董阳. 一重和三重脑震荡鼠BB、BW和FS行为学变化及脑组织GFAP和MBP变化研究[D]. 昆明: 昆明医科大学, 2013.
8. Marmarou A, Foda MA, van den Brink W, et al. A new model of diffuse brain injury in rats. Part I: Pathophysiology and biomechanics[J]. J Neurosurg, 1994, 80(2): 291-300.
9. 陈锐, 严治, 胡玲, 等. 大鼠脑震荡模型建立法的改良和评价[J]. 广东医学院学报, 2010, 28(4): 362-363.
10. Kamijo R, Harada H, Matsuyama T, et al. Requirement for transcription factor IRF-1 in NO synthase induction in macrophages[J]. Science, 1994, 263(5153): 1612-1615.
11. Saura M, Zaragoza C, Bao G, et al. Interaction of interferon regulatory factor-1 and nuclear factor kappa B during activation of inducible nitric oxide synthase transcription[J]. J Mol Biol, 1999, 289(3): 459-471.
12. Martin E, Nathan C, Xie Q. Role of interferon regulatory factor 1 in induction of nitric oxide synthase[J]. J Exp Med, 1994, 180(3): 977-984.
13. Xie K, Huang S, Dong Z, et al. Destruction of bystander cells by tumor cells transfected with inducible nitric oxide (NO) synthase gene[J]. J Natl Cancer Inst, 1997, 89(6): 421-427.
14. Robertson FM, Long BW, Tober KL, et al. Gene expression and cellular sources of inducible nitric oxide synthase during tumor promotion[J]. Carcinogenesis, 1996, 17(9): 2053-2059.
15. Palmieri D, Capponi S, Geroldi A, et al. TNF alpha induces the expression of genes associated with endothelial dysfunction through p38MAPK-mediated down-regulation of miR-149[J]. Biochem Biophys Res Commun, 2014, 443(1): 246-251.

West China Medical Journal

Comparison of Evaluation in vitro between Erhuang Gel Paste and Its Ointment

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