The Expression of Yes-associated Protein Based on Bioinformatics in Rats with Myocardial-ischemia Reperfusion Injury_West China Medical Journal

Authors：

 HuYingchun , ZhongWu , ChenMuhu , LiShaolan , LiLinfang

Emergency Department, the Affiliated Hospital of Southwest Medical University, Luzhou, Sichuan 646000, P. R. China;

Corresponding author：

Keywords：

Myocardial-ischemia reperfusion injury; HIPPO signaling pathway; Yes-associated protein; Bioinformatics; Rat

DOI：

10.7507/1002-0179.201600272

Video：

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Objective To investigate the expression of Yes-associated protein (YAP) screened by bioinformatics in rats with myocardial-ischemia reperfusion injury and establish the base for further research. Methods The difference of gene spectrum of rats with myocardial-ischemia reperfusion injury was analyzed by bioinformatics technique. The related signaling pathways and key genes were screened by KOBAS2.0 and KEGG. Eighteen Sprague Dawley rats were randomly divided into three groups: normal group (n=6), sham operation group (n=6) and myocardial-ischemia reperfusion injury group (n=6). The expression of target gene was detected by immunochemistry, quantitive reverse transcription polymerase chain reaction and western blotting. Results A total of 345 differentially expressed genes were found by bioinformatics, among which 181 were up-regulated and 164 were down-regulated. The differential genes were mainly enriched in Wnt, HIPPO, MAPK, Jak-STAT and other signaling pathways. We focused on HIPPO pathway and found that the expression of YAP increased significantly in myocardial-ischemia reperfusion injury group, compared with the normal group and sham operation group (P＜0.05). Conclusions The expression of YAP of HIPPO signal pathway is increased in rats with myocardial-ischemia reperfusion injury.

Citation： HuYingchun, ZhongWu, ChenMuhu, LiShaolan, LiLinfang. The Expression of Yes-associated Protein Based on Bioinformatics in Rats with Myocardial-ischemia Reperfusion Injury. West China Medical Journal, 2016, 31(6): 1008-1012. doi: 10.7507/1002-0179.201600272 Copy

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13.	Harvey KF, Zhang X, Thomas DM. The hippo pathway and human cancer[J]. Nat Rev Cancer, 2013, 13(4): 246-257.
14.	Johnson R, Halder G. The two faces of Hippo: targeting the Hippo pathway for regenerative medicine and cancer treatment[J]. Nat Rev Drug Discov, 2014, 13(1): 63-79.
15.	Li N, Lim G, Chen L, et al. Spinal expression of Hippo signaling components YAP and TAZ following peripheral nerve injury in rats[J]. Brain Res, 2013, 1535: 137-147.
16.	Orcholski ME, Zhang Q, Bredesen DE. Signaling via amyloid precursor-like proteins APLP1 and APLP2[J]. J Alzheimers Dis, 2011, 23(4): 689-699.
17.	Heallen T, Zhang M, Wang J, et al. Hippo pathway inhibits Wnt signaling to restrain cardiomyocyte proliferation and heart size[J]. Science, 2011, 332(628): 458-461.
18.	Xin M, Kim Y, Sutherland LB, et al. Hippo pathway effector Yap promotes cardiac regeneration[J]. Proc Natl Acad Sci USA, 2013, 110(34): 13839-13844.
19.	Schlegelmilch K, Mohseni M, Kirak O, et al. Yap1 acts downstream of α-catenin to control epidermal proliferation[J]. Cell, 2011, 144(5): 782-795.

1. Zhou T, Chuang CC, Zuo L. Molecular characterization of reactive oxygen species in myocardial ischemia-reperfusion injury[J]. Biomed Res Int, 2015, 2015: 864946.
2. Yang Q, He GW, Underwood MJ, et al. Cellular and molecular mechanisms of endothelial ischemia/reperfusion injury: perspectives and implications for postischemic myocardial protection[J]. Am J Transl Res, 2016, 8(2): 765-777.
3. Vilahur G, Badimon L. Ischemia/reperfusion activates myocardial innate immune response: the key role of the toll-like receptor[J]. Front Physiol, 2014, 5: 496.
4. Zeng XC, Li L, Wen H, et al. MicroRNA-128 inhibition attenuates myocardial ischemia/reperfusion injury-induced cardiomyocyte apoptosis by the targeted activation of peroxisome proliferatoractivated receptor gamma[J/OL]. Mol Med Rep, 2016: 129-136. [2016-06-08]. https://www.spandidos-publications.com/10.3892/mmr.2016.5208..
5. Roy S, Khanna S, Kuhn DE, et al. Transcriptome analysis of the ischemia-reperfused remodeling myocardium: temporal changes in inflammation and extracellular matrix[J]. Physiol Genomics, 2006, 25(3): 364-374.
6. Xie C, Mao X, Huang J, et al. KOBAS 2.0: a web server for annotation and identification of enriched pathways and diseases[J]. Nucleic Acids Res, 2011, 39(Web Server issue): W316-W322.
7. Chua S, Lee FY, Tsai TH, et al. Inhibition of dipeptidyl peptidase-Ⅳ enzyme activity protects against myocardial ischemia-reperfusion injury in rats[J]. J Transl Med, 2014, 12: 357.
8. Ke LD, Chen Z, Yung WK. A reliability test of standard-based quantitative PCR: exogenous vs endogenous standards[J]. Mol Cell Probes, 2000, 14(2): 127-135.
9. Liu W, Saint DA. Validation of a quantitative method for real time PCR kinetics[J]. Biochem Biophys Res Commun, 2002, 294(2): 347-353.
10. Logan CY, Nusse R. The Wnt signaling pathway in development and disease[J]. Annu Rev Cell Dev Biol, 2004, 20: 781-810.
11. Keshet Y, Seger R. The MAP kinase signaling cascades: a system of hundreds of components regulates a diverse array of physiological functions[J]. Methods Mol Biol, 2010, 661: 3-38.
12. Nicolas CS, Peineau S, Amici M, et al. The Jak/STAT pathway is involved in synaptic plasticity[J]. Neuron, 2012, 73(2): 374-390.
13. Harvey KF, Zhang X, Thomas DM. The hippo pathway and human cancer[J]. Nat Rev Cancer, 2013, 13(4): 246-257.
14. Johnson R, Halder G. The two faces of Hippo: targeting the Hippo pathway for regenerative medicine and cancer treatment[J]. Nat Rev Drug Discov, 2014, 13(1): 63-79.
15. Li N, Lim G, Chen L, et al. Spinal expression of Hippo signaling components YAP and TAZ following peripheral nerve injury in rats[J]. Brain Res, 2013, 1535: 137-147.
16. Orcholski ME, Zhang Q, Bredesen DE. Signaling via amyloid precursor-like proteins APLP1 and APLP2[J]. J Alzheimers Dis, 2011, 23(4): 689-699.
17. Heallen T, Zhang M, Wang J, et al. Hippo pathway inhibits Wnt signaling to restrain cardiomyocyte proliferation and heart size[J]. Science, 2011, 332(628): 458-461.
18. Xin M, Kim Y, Sutherland LB, et al. Hippo pathway effector Yap promotes cardiac regeneration[J]. Proc Natl Acad Sci USA, 2013, 110(34): 13839-13844.
19. Schlegelmilch K, Mohseni M, Kirak O, et al. Yap1 acts downstream of α-catenin to control epidermal proliferation[J]. Cell, 2011, 144(5): 782-795.

West China Medical Journal

The Expression of Yes-associated Protein Based on Bioinformatics in Rats with Myocardial-ischemia Reperfusion Injury

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