The Effects of Aucklandia and Coptis Pills on Cellular Apoptosis and the Expression of Bcl-2 and Bax mRNA in Model Rats with Ulcerative Colitis_West China Medical Journal

Authors：

 DongYan , LuJingen

Institute of Traditional Chinese Medicine Surgery, Longhua Hosipital Affiliated to Shanghai University of Traditional Chinese Medicine, Shanghai 200032, P. R. China;

Corresponding author：

Keywords：

Ulcerative colitis; Apoptosis; Bcl-2 mRNA; Bax mRNA; Rat

DOI：

10.7507/1002-0179.201600281

Video：

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Objective To investigate the effects of Aucklandia and Coptis pills on cellular apoptosis and the expression of Bcl-2 and Bax mRNA in model rats with ulcerative colitis (UC). Methods Fifty male Wistar rats at the age of seven weeks were randomly divided into five groups: control group, model group, Chinese medicine group (Aucklandia and Coptis pills), inhibitor group, and Chinese medicine plus inhibitor group. The experiment was performed with rats of UC induced by trinitro-benzene-sulfonic acid enema. AG-490 and Aucklandia and Coptis pills were administrated to them by intraperitoneal injection and gavage. Colonic mucosal tissues in rats of all the five groups were observed and evaluated by light microscope. Cellular apoptosis in colonic mucosal tissues was detected by TUNEL. The expressions of Bcl-2 and Bax mRNA were detected by reverse transcription polymerase chain reaction. Results There were many ulcers in the colon of UC rats, and pathological changes in colonic mucosa such as inflammation, congestion and edema were observed in the colon of UC model rats by naked eye and microscope. Compared with UC rats without treatment, Aucklandia and Coptis pills alleviated colonic mucosal injuries and decreased apoptosis rate of colonic epithelial cells, while the expression of Bax mRNA was decreased in the colonic mucosa in UC rats treated with Aucklandia and Coptis pills, and Bcl-2 mRNA expression was increased. Conclusion Aucklandia and Coptis pills can effectively inhibit mRNA expression of apoptosisrelated molecules to down-regulate colonic epithelial cells apoptosis in colonic mucosa in rats with UC.

Citation： DongYan, LuJingen. The Effects of Aucklandia and Coptis Pills on Cellular Apoptosis and the Expression of Bcl-2 and Bax mRNA in Model Rats with Ulcerative Colitis. West China Medical Journal, 2016, 31(6): 1046-1051. doi: 10.7507/1002-0179.201600281 Copy

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8.	Arab HH, Al-Shorbagy MY, Abdallah DM, et al. Telmisartan attenuates colon inflammation, oxidative perturbations and apoptosis in a rat model of experimental inflammatory bowel disease[J]. PLoS One, 2014, 9(5): e97193.
9.	Xu L, He SS, Li DY, et al. Hydrogen peroxide induces oxidative stress and the mitochondrial pathway of apoptosis in RAT intestinal epithelial cells (IEC-6) [J]. Mol Biol (Mosk), 2016, 50(2): 311-319.
10.	Reed JC. Proapoptotic multidomain Bcl-2/Bax-family proteins: mechanisms, physiological roles, and therapeutic opportunities[J]. Cell Death Differ, 2006, 13(8): 1378-1386.
11.	Ouyang H, Furukaw a T, Abe T, et al. The BAX gene, the promoter of apoptosis, is mutated in genetically unstable cancers of the colorectum, stomach, and endometrium[J]. Clin Cancer Res, 1998, 4(4): 1071-1074.
12.	Zhao HM, Huang XY, Zhou F, et al. Si Shen Wan inhibits mRNA expression of apoptosis-related molecules in p38 MAPK signal pathway in mice with colitis[J]. Evid Based Complement Alternat Med, 2013, 2013: 432097.
13.	Limura M, Nakamura T, Shinozaki S, et al. Bax is down regulated in inflamed colonic mucosa of ulcerative colitis[J]. Gut, 2000, 47(2): 228-235.
14.	Alas S, Bonavida B. Rituximab inactivates signal transducer and activation of transcription 3 (STAT3) activity in B-non- Hodgkin’s lymphoma through inhibition of the interleukin 10 autocrine/paracrine loop and results in down-regulation of Bcl-2 and sensitization to cytotoxic drugs[J]. Cancer Res, 2001, 61(13): 5137-5144.
15.	张文俊, 许国铭, 李兆申, 等. 细胞凋亡调控蛋白Bcl-2和Bax在溃疡性结肠炎表达的研究[J]. 中华消化内镜杂志, 2003, 23(3): 189-192.
16.	Karamanolis DG, Kyrlagkitsis I, Konstantinou K, et al. The Bcl-2/Bax system and apoptosis in ulcerative colitis[J]. Hepatogastroenterology, 2007, 54(76): 1085-1088.

1. Morris GP, Beck PL, Herridge MS, et al. Hapten-induced model of chronic inflammation and ulceration in the rat colon[J]. Gastroenterology, 1989, 96(3): 795-803.
2. Becker C, Watson AJ, Neurath MF. Complex roles of caspases in the pathogenesis of inflammatory bowel disease[J]. Gastroenterology, 2013, 144(2): 283-293.
3. 王红伟, 徐华明. 针刺对局灶性脑缺血再灌注大鼠脑细胞凋亡的影响[J]. 河南中医学院学报, 2007, 27(6): 21-22.
4. 范恒, 李小慧, 段雪云, 等. 细胞凋亡与UC的发生[J]. 中国中西医结合消化杂志, 2002, 10(3): 189-190.
5. Mahendran V, Liu F, Riordan SM, et al. Examination of the effects of Campylobacter concisus zonula occludens toxin on intestinal epithelial cells and macrophages[J]. Gut Pathog, 2016, 18(8): 18.
6. 余保平, 王伟岸. 消化系统疾病免疫学[M]. 北京: 科学出版社, 2000: 189-190.
7. Halpert G, Eitan T, Voronov E, et al. Multifunctional activity of a small tellurium redox immunomodulator compound, AS101, on dextran sodium sulfate-induced murine colitis[J]. J Biol Chem, 2014, 289(24): 17215-17227.
8. Arab HH, Al-Shorbagy MY, Abdallah DM, et al. Telmisartan attenuates colon inflammation, oxidative perturbations and apoptosis in a rat model of experimental inflammatory bowel disease[J]. PLoS One, 2014, 9(5): e97193.
9. Xu L, He SS, Li DY, et al. Hydrogen peroxide induces oxidative stress and the mitochondrial pathway of apoptosis in RAT intestinal epithelial cells (IEC-6) [J]. Mol Biol (Mosk), 2016, 50(2): 311-319.
10. Reed JC. Proapoptotic multidomain Bcl-2/Bax-family proteins: mechanisms, physiological roles, and therapeutic opportunities[J]. Cell Death Differ, 2006, 13(8): 1378-1386.
11. Ouyang H, Furukaw a T, Abe T, et al. The BAX gene, the promoter of apoptosis, is mutated in genetically unstable cancers of the colorectum, stomach, and endometrium[J]. Clin Cancer Res, 1998, 4(4): 1071-1074.
12. Zhao HM, Huang XY, Zhou F, et al. Si Shen Wan inhibits mRNA expression of apoptosis-related molecules in p38 MAPK signal pathway in mice with colitis[J]. Evid Based Complement Alternat Med, 2013, 2013: 432097.
13. Limura M, Nakamura T, Shinozaki S, et al. Bax is down regulated in inflamed colonic mucosa of ulcerative colitis[J]. Gut, 2000, 47(2): 228-235.
14. Alas S, Bonavida B. Rituximab inactivates signal transducer and activation of transcription 3 (STAT3) activity in B-non- Hodgkin’s lymphoma through inhibition of the interleukin 10 autocrine/paracrine loop and results in down-regulation of Bcl-2 and sensitization to cytotoxic drugs[J]. Cancer Res, 2001, 61(13): 5137-5144.
15. 张文俊, 许国铭, 李兆申, 等. 细胞凋亡调控蛋白Bcl-2和Bax在溃疡性结肠炎表达的研究[J]. 中华消化内镜杂志, 2003, 23(3): 189-192.
16. Karamanolis DG, Kyrlagkitsis I, Konstantinou K, et al. The Bcl-2/Bax system and apoptosis in ulcerative colitis[J]. Hepatogastroenterology, 2007, 54(76): 1085-1088.

West China Medical Journal

The Effects of Aucklandia and Coptis Pills on Cellular Apoptosis and the Expression of Bcl-2 and Bax mRNA in Model Rats with Ulcerative Colitis

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