Antinuclear Antibodies Assay: A Comparative Study between HELIOS and Artificial Interpretation_West China Medical Journal

Authors：

 HuJing , LiLixin , LuoLimei , LiYi , HuangZhuochun , PengXiaodong ,  WuYongkang

Department of Laboratory Medicine, West China Hospital, Sichuan University, Chengdu, Sichuan 610041, P. R. China;

Corresponding author：

WuYongkang, Email: vipwyk@163.com

Keywords：

Antinuclear antibodies; Indirect immunofluorescence; Comparison analysis

DOI：

10.7507/1002-0179.201600291

Video：

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Abstract Full text Figures/Tables Video References Cited by

Objective To verify the consistency between artificial interpretation and automatic interpretation by HELIOS automatic immunofluorescence system by comparing their results on the same antinuclear antibodies (ANA) fluorescent slides, and analyze the application of automatic interpretation clinically. Method A total of 281 ANA fluorescent slides of 281 impatients or outpatients in February 2015 were analyzed by HELIOS automatic immunofluorescence system and artificial interpretation respectively. As HELIOS could only determine the titer not the fluorescence type, only the negative or positive results qualitatively and the titer of ANA positive slides were analyzed. Results There was no statistically significant difference between HELIOS automatic immunofluorescence system and artificial interpretation in negative or positive rate qualitatively (P>0.05) . The total coincidence rate was 98.9%, the positive coincidence rate was 99.5%, and the negative coincidence rate was 97.4%, and the kappa coefficient was 0.973. The difference of titer between the two groups had no statistical significance (P>0.05) . Conclusions The results of HELIOS automatic Immunofluorescence system and artificial interpretation are in good consistency. HELIOS automatic immunofluorescence system is suitable for clinical use as its high degree of automation, simple operation and result reliability.

Citation： HuJing, LiLixin, LuoLimei, LiYi, HuangZhuochun, PengXiaodong, WuYongkang. Antinuclear Antibodies Assay: A Comparative Study between HELIOS and Artificial Interpretation. West China Medical Journal, 2016, 31(6): 1084-1087. doi: 10.7507/1002-0179.201600291 Copy

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13.	Bizzaro N, Antico A, Platzgummer S, et al. Automated antinuclear immunofluorescence antibody screening:a comparative study of six computer-aided diagnostic systems[J]. Autoimmun Rev, 2014, 13(3) :292-298.
14.	Wiik AS, Bizzaro N. Missing links in high quality diagnostics of inflammatory systemic rheumatic diseases[J]. Auto Immun Highlights, 2012, 3(2) :35-49.
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1. Wieser M, Pohla-Gubo G, Hintner H. Antinuclear antibodies (ANA) Diagnostic value of different methods for screening and differentiation[J]. Clin Appled Immunol Rev, 2001, 1(3) :201-206.
2. 谢兰, 程京. 生物芯片的临床应用现状和未来发展趋势[J]. 中华检验医学杂志, 2010, 33(11) :1000-1010.
3. Fritzler MJ. Toward a new autoantibody diagnostic orthodoxy: understanding the bad, good and indifferent[J]. Auto Immu Highlights, 2012, 3(2) :51-58.
4. Copple SS. Screening for IgG antinuclear autoantibodies by HEp-2 indirect fluorescent antibody assays and the need for standardization[J]. Am J Clin Pathol, 2012, 137(5) :825-830.
5. Bizzaro N, Tozzoli R, Tonutti E, et al. Variability between methods to determine ANA, anti-dsDNA and anti-ENA autoantibodies: a collaborative study with the biomedical industry[J]. J Immunol Methods, 1998, 219:99-107.
6. Boland MV, Murphy RF. A neural network classifier capable of recognizing the patterns of all major subcellular structures in fluorescence microscope images of HeLa cells[J]. Bioinformatics, 2002, 17(12) :1213-1223.
7. Bossuyt X, Luyckx A. Antibodies to extractable nuclear antigens in antinuclear antibody-negative samples[J]. Clin Chem, 2005, 51(12) : 2426-2427.
8. 叶应妩, 王毓三, 申子瑜. 全国临床检验操作规程[M]. 3版.南京: 东南大学出版社, 2006:655-658.
9. Tozzoli R, Bonaguri C, Melegari A, et al. Current state of diagnostic technologies in the autoimmunology laboratory[J]. Clin Chem Lab Med, 2013, 51(1) :129-138.
10. Solomon DH, Kavanaugh AJ, Schur PH. Evidence-based guidelines for the use of immunologic tests:antinuclear antibody testing[J]. Arthritis Rheum, 2002, 47(4) :434-444.
11. Almeida González D, Cabrera de LeÓn A, Rodríguez Pérez Mdel C, et al. Efficiency of different strategies to detect autoantibodies to extractable nuclear antigens[J]. J Immunol Methods, 2010, 360(1/2) : 89-95.
12. Kavanaugh A, Tomar R, Reveille J. et al. Guidelines for clinical use of the antinuclear antibody test and tests for specific autoantibodies to nuclear antigens[J]. Arch Pathol Lab Med, 2000, 124(1) :71-81.
13. Bizzaro N, Antico A, Platzgummer S, et al. Automated antinuclear immunofluorescence antibody screening:a comparative study of six computer-aided diagnostic systems[J]. Autoimmun Rev, 2014, 13(3) :292-298.
14. Wiik AS, Bizzaro N. Missing links in high quality diagnostics of inflammatory systemic rheumatic diseases[J]. Auto Immun Highlights, 2012, 3(2) :35-49.
15. Fritzler MJ. The antinuclear antibody test:last or lasting gasp[J]. Arthritis Rheum, 2010, 63(1) :19-22.

West China Medical Journal

Antinuclear Antibodies Assay: A Comparative Study between HELIOS and Artificial Interpretation

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