健脾化湿法对特应性皮炎小鼠皮肤水通道蛋白3表达的影响_West China Medical Journal

Authors：

 周爱妍 ¹ ,  杜迎 ² , 史传奎 ¹

1. ;
2. ;

Corresponding author：

杜迎, Email: duying0813@126.com

Keywords：

特应性皮炎; 健脾化湿法; 水通道蛋白

DOI：

10.7507/1002-0179.20160033

Video：

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目的观察健脾化湿法对特应性皮炎（AD）小鼠水通道蛋白3（AQP3）表达的影响，探讨健脾化湿法治疗AD的作用机制与途径，为进一步阐明健脾化湿法治疗AD的分子生物学提供依据。方法将30只7周龄昆明小鼠随机分为中药模型组、空白模型组、空白对照组，每组各10只。中药模型组和空白模型组建立皮炎模型，建模后中药模型组采用小儿健脾化湿水煎液灌胃，剂量为10 mL/kg，2次/d，空白模型组以等量生理盐水灌胃；空白对照组不建立皮炎模型，以等量生理盐水灌胃。小鼠鼠龄达11周龄同时取其背部皮肤组织采用免疫组织化学法（PV法）检测AD小鼠皮肤AQP3的表达及分布。结果中药模型组、空白模型组、空白对照组小鼠皮肤组织中AQP3吸光度值分别为0.450±0.061、0.611±0.063、0.261±0.040，空白模型组高于空白对照组，差异有统计学意义（P＜0.01）；中药模型组低于空白模型组，差异有统计学意义（P＜0.01）。结论健脾化湿法可以明显改善AD小鼠皮肤组织中AQP3的表达与分布，促进皮肤组织的修复。

Citation： 周爱妍, 杜迎, 史传奎. 健脾化湿法对特应性皮炎小鼠皮肤水通道蛋白3表达的影响. West China Medical Journal, 2016, 31(1): 119-121. doi: 10.7507/1002-0179.20160033 Copy

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5.	Pokharel YR, Lim SC, Kim SC, et al. Sopungyangiac-Tang inhibits development of dermatitis in Nc/Nga mice[J]. Evid Based Complement Altemat Med, 2008, 5(2):173-180.
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7.	Qi XF, Kim DH, Yoon YS, et al. Effects of bambusae caulis in liquamen on the development of atopic dermatitis-like skin lesions in hairless mice[J]. Ethnopharmaeol, 2009, 123(2):195-200.
8.	陈奇. 中药药理研究方法学[M]. 2版. 北京:人民卫生出版社, 2006:31.
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10.	Wang KS, Ma TH, Filiz F, et al. Colon water transport in transgenic mice lacking aquaporin-4 water channels[J]. Am J Physiol Gastrointest Liver Physiol, 2000, 279(2):G463-G470.
11.	Toshiyuki, Takeshi S. Water Channel protein AQP3 is present in epithelia exposed to the environment of possible water loss[J]. Histochem Soc, 1999, 47(10):1275-1286.
12.	Agren J. Transepidermal water loss in developing rats:role of aquaporins in the immature skin[J]. J Pediatr Res, 2003, 53(4): 558-565.
13.	Warner RR, Myers MC, Taylor DA. Electron probe analysis of human skin:determination of the water concentration profile[J]. J Invest Dermatol, 1988, 90:218-224.
14.	Olsson M, Broberg A, Jernås M, et al. Increased expression of aquaporin 3 in atopic eczema[J]. Allergy, 2006, 61(9):1132-1137.

1. 陈保疆, 张玉环. 健脾利湿汤治疗异位性皮炎的疗效观察与相关实验研究[J]. 吉林中医药, 2011, 31(4):335-336.
2. 石汉振, 曾冬玉, 陈达灿, 等. 健脾渗湿法治疗特应性皮炎患者血清总IgE、IgG、IgG4变化及疗效评价[J]. 中国中西医结合皮肤性病学杂志, 2011, 10(1):20-22.
3. 谭远忠, 魏文斌, 刘华, 等. 运脾化湿中药对肠易激综合征脾胃湿阻证患者AQP8表达的影响研究[J]. 中药材, 2009, 32(9):1487-1490.
4. 宋秀祖, 宋为民, 许爱娥, 等. AQP3、Caspase14、博来霉素水解酶对慢性湿疹皮肤屏障的作用研究[J]. 中华皮肤科杂志, 2012, 45(2):83-86.
5. Pokharel YR, Lim SC, Kim SC, et al. Sopungyangiac-Tang inhibits development of dermatitis in Nc/Nga mice[J]. Evid Based Complement Altemat Med, 2008, 5(2):173-180.
6. Park CW, Youn M, Jung YM, et al. New functional probiotic Lactobacillus sakei probio 65 alleviates atopic symptoms in the mouse[J]. J Med Food, 2008, 11(3):405-412.
7. Qi XF, Kim DH, Yoon YS, et al. Effects of bambusae caulis in liquamen on the development of atopic dermatitis-like skin lesions in hairless mice[J]. Ethnopharmaeol, 2009, 123(2):195-200.
8. 陈奇. 中药药理研究方法学[M]. 2版. 北京:人民卫生出版社, 2006:31.
9. 李辉, 高强, 王翼腾, 等. 应用小鼠建立特应性皮炎模型的研究进展[J]. 武警医学, 2015, 26(1):99-102.
10. Wang KS, Ma TH, Filiz F, et al. Colon water transport in transgenic mice lacking aquaporin-4 water channels[J]. Am J Physiol Gastrointest Liver Physiol, 2000, 279(2):G463-G470.
11. Toshiyuki, Takeshi S. Water Channel protein AQP3 is present in epithelia exposed to the environment of possible water loss[J]. Histochem Soc, 1999, 47(10):1275-1286.
12. Agren J. Transepidermal water loss in developing rats:role of aquaporins in the immature skin[J]. J Pediatr Res, 2003, 53(4): 558-565.
13. Warner RR, Myers MC, Taylor DA. Electron probe analysis of human skin:determination of the water concentration profile[J]. J Invest Dermatol, 1988, 90:218-224.
14. Olsson M, Broberg A, Jernås M, et al. Increased expression of aquaporin 3 in atopic eczema[J]. Allergy, 2006, 61(9):1132-1137.

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健脾化湿法对特应性皮炎小鼠皮肤水通道蛋白3表达的影响

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