Detection and Correlation Analysis between Anti-cell Membrane DNA Antibody and Other Autoantibodies_West China Medical Journal

Authors：

 HuJing ¹ , LiLixin ¹ , WuYongkang ¹ , WeiDapeng ² ,  PengXiaodong ¹

1. Department of Laboratory Medicine, West China Hospital, Sichuan University, Chengdu 610041, P. R. China;
2. Teaching and Research Section of Immunology, College of Basic and Forensic Medicine, Sichuan University, Chengdu 610041, P. R. China;

Corresponding author：

PengXiaodong, Email: pxd@scu.edu.cn

Keywords：

Anti-cell membrane DNA antibody; Systemic lupus erythematosus; Correlation; Sensitivity; Specificity

DOI：

10.7507/1002-0179.201600374

Video：

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Objective To analyze the correlation between anti-cell membrane DNA (mDNA) antibodies and other autoantibodies and estimate its diagnosing significance for systemic lupus erythematosus (SLE). Methods From January to August 2015, the sera samples from 254 patients with various autoimmune diseases, including 106 SLE, 80 rheumatoid arthritis (RA), 32 mixed connective tissue disease (MCTD), 29 Sjogren's syndrome (SS), 7 polymyositis/dermatomyositis (PM/DM) and 20 healthy controls, were collected. The anti-mDNA antibody, anti-dsDNA antibody, antinuclear antibody (ANA) and anti-keratin antibody (AKA) were detected by indirect immunofluorescent assay; anti-cyclic citrylinated peptide antibody (CCP) antibody was detected by enzyme-linked immuno sorbent assay; rheumatoid factor (RF) was detected by rat scatter turbidimetry assay; and anti-Sm antibody was detected by Western blotting method. Results Anti-mDNA antibody was found in 77 of 106 SLE (72.6%), 4 of 80 RA (5.0%), 6 of 32 MCTD (18.7%), 4 of 29 SS (14.7%), 0 of 7 PM/DM (0.0%) and 0 of 20 healthy controls (0.0%), respectively. It's notable higher in SLE than that in the others (P < 0.001). The sensitivity, specificity and diagnosis efficiency of anti-mDNA antibody for SLE were 72.6%, 91.7% and 84.3%, respectively. Anti-mDNA antibody was significantly correlated with ANA, anti-dsDNA antibody and anti-Sm antibody (P < 0.001), while it had no significant correlation with anti-CCP antibody, AKA and RF (P > 0.05). Conclusion Anti-mDNA antibody is closely related with other SLE associated antibodies and with high sensitivity and specificity for SLE diagnosis.

Citation： HuJing, LiLixin, WuYongkang, WeiDapeng, PengXiaodong. Detection and Correlation Analysis between Anti-cell Membrane DNA Antibody and Other Autoantibodies. West China Medical Journal, 2016, 31(8): 1372-1375. doi: 10.7507/1002-0179.201600374 Copy

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2.	Ching KH, Burbelo PD, Tipton C, et al. Two major autoantibody clusters in systemic lupus erythematosus[J]. PLoS One, 2012, 7(2):e32001.
3.	Pisetsky DS. Anti-DNA antibodies——quintessential biomarkers of SLE[J]. Nat Rev Rheumatol, 2016, 12(2):102-110.
4.	Servais G, Guillaume MP, Dumarey N, et al. Evidence of autoantibodies to cell membrane associated DNA (cultured lymphocytes):a new specific marker for rapid identification of systemic lupus erythematosus[J]. Ann Rheum Dis, 1998, 57(10):606-613.
5.	Bennett RM, Kotzin BL, Merritt MJ. DNA receptor dysfunction in systemic lupus erythematosus and kindred disorders. Induction by anti-DNA antibodies, antihistone antibodies, and antireceptor antibodies[J]. J Exp Med, 1987, 166(4):850-863.
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7.	郭嘉隆, 毕黎琦, 栗占国.抗细胞膜DNA抗体在三种自身抗体阴性系统性红斑狼疮中的诊断价值[J].中华检验医学杂志, 2006, 29(9):787-790.
8.	茹晋丽, 魏华, 吕志勤, 等.儿童系统性红斑狼疮细胞膜DNA抗体间接免疫荧光检测研究[J].中华儿科杂志, 2009, 47(11):820-823.
9.	Alspaugh MA, Tan EM. Antibodies to cellular antigens in Sjögren's syndrome[J]. J Clin Invest, 1975, 55(55(5)):1067-1073.
10.	Ru JL, Zhao Y, Xie XX, et al. Clinical applications of the indirect immunofluorescence assay for detection of anticell membrane-associated DNA antibodies in juvenile systemic lupus erythematosus[J]. Pediatr Res, 2015, 77(2):376-380.
11.	Chen HY, Guo JL, Li ZG. Significance of anti-cell membrane-associated DNA (mDNA) antibodies in systemic lupus erythematosus[J]. Clin Rheumatol, 2008, 27(2):183-187.
12.	Servais G, Daens S, Guillaume MP, et al. Diagnostic specificities and sensitivities of anti dsDNA, anti membrane DNA and anti nucleosomes autoantibodies[J]. Scand J Clin Lab Invest Suppl, 2001, 235(2):61-67.
13.	陈海英, 贾汝琳, 栗占国.抗细胞膜DNA抗体的测定及其在系统性红斑狼疮诊断中的意义[J].中华风湿病学杂志, 2005, 9(4):229-233.
14.	James JA, Robertson JM. Lupus and Epstein-Barr[J]. Curr Opin Rheumatol, 2012, 24(4):383-388.
15.	Larsen M, Sauce D, Deback C, et al. Exhausted cytotoxic control of Epstein-Barr virus in human lupus[J]. PLoS Pathog, 2011, 7(10):e1002328.
16.	Mcclain MT, Poole BD, Bruner BF, et al. An altered immune response to Epstein-Barr nuclear antigen 1 in pediatric systemic lupus erythematosus[J]. Arthritis Rheum, 2006, 54(1):360-368.
17.	Incaprera M, Rindi L, Bazzichi A, et al. Potential role of the Epstein-Barr virus in systemic lupus erythematosus autoimmunity[J]. Clin Exp Rheumatol, 1998, 16(3):289-294.

1. Binder E, Edelbauer M. Use of biomarkers in the management of children with lupus[J]. Curr Rheumatol Rep, 2013, 15(3):312.
2. Ching KH, Burbelo PD, Tipton C, et al. Two major autoantibody clusters in systemic lupus erythematosus[J]. PLoS One, 2012, 7(2):e32001.
3. Pisetsky DS. Anti-DNA antibodies——quintessential biomarkers of SLE[J]. Nat Rev Rheumatol, 2016, 12(2):102-110.
4. Servais G, Guillaume MP, Dumarey N, et al. Evidence of autoantibodies to cell membrane associated DNA (cultured lymphocytes):a new specific marker for rapid identification of systemic lupus erythematosus[J]. Ann Rheum Dis, 1998, 57(10):606-613.
5. Bennett RM, Kotzin BL, Merritt MJ. DNA receptor dysfunction in systemic lupus erythematosus and kindred disorders. Induction by anti-DNA antibodies, antihistone antibodies, and antireceptor antibodies[J]. J Exp Med, 1987, 166(4):850-863.
6. Bennett RM, Davis J, Merritt M. Anti-DNA antibodies react with DNA expressed on the surface of monocytes and B lymphocytes[J]. J Rheumatol, 1986, 13(4):679-685.
7. 郭嘉隆, 毕黎琦, 栗占国.抗细胞膜DNA抗体在三种自身抗体阴性系统性红斑狼疮中的诊断价值[J].中华检验医学杂志, 2006, 29(9):787-790.
8. 茹晋丽, 魏华, 吕志勤, 等.儿童系统性红斑狼疮细胞膜DNA抗体间接免疫荧光检测研究[J].中华儿科杂志, 2009, 47(11):820-823.
9. Alspaugh MA, Tan EM. Antibodies to cellular antigens in Sjögren's syndrome[J]. J Clin Invest, 1975, 55(55(5)):1067-1073.
10. Ru JL, Zhao Y, Xie XX, et al. Clinical applications of the indirect immunofluorescence assay for detection of anticell membrane-associated DNA antibodies in juvenile systemic lupus erythematosus[J]. Pediatr Res, 2015, 77(2):376-380.
11. Chen HY, Guo JL, Li ZG. Significance of anti-cell membrane-associated DNA (mDNA) antibodies in systemic lupus erythematosus[J]. Clin Rheumatol, 2008, 27(2):183-187.
12. Servais G, Daens S, Guillaume MP, et al. Diagnostic specificities and sensitivities of anti dsDNA, anti membrane DNA and anti nucleosomes autoantibodies[J]. Scand J Clin Lab Invest Suppl, 2001, 235(2):61-67.
13. 陈海英, 贾汝琳, 栗占国.抗细胞膜DNA抗体的测定及其在系统性红斑狼疮诊断中的意义[J].中华风湿病学杂志, 2005, 9(4):229-233.
14. James JA, Robertson JM. Lupus and Epstein-Barr[J]. Curr Opin Rheumatol, 2012, 24(4):383-388.
15. Larsen M, Sauce D, Deback C, et al. Exhausted cytotoxic control of Epstein-Barr virus in human lupus[J]. PLoS Pathog, 2011, 7(10):e1002328.
16. Mcclain MT, Poole BD, Bruner BF, et al. An altered immune response to Epstein-Barr nuclear antigen 1 in pediatric systemic lupus erythematosus[J]. Arthritis Rheum, 2006, 54(1):360-368.
17. Incaprera M, Rindi L, Bazzichi A, et al. Potential role of the Epstein-Barr virus in systemic lupus erythematosus autoimmunity[J]. Clin Exp Rheumatol, 1998, 16(3):289-294.

West China Medical Journal

Detection and Correlation Analysis between Anti-cell Membrane DNA Antibody and Other Autoantibodies

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