Optimization of the Extracting Process of Zipu Gouty Mixture by Orthogonal Design_West China Medical Journal

Authors：

 LiuZhongjun ¹ ,  FengGuangfu ¹ , PiFengjuan ² , TuHe ¹ , ZhuLiyang ¹ , PangChaoping ¹

1. Department of Pharmacy, the Orthopedic Hospital of Sichuan Province, Chengdu, Sichuan 610041, P. R. China;
2. Department of Pharmacy, Luzhou Hospital of Traditional Chinese Medicine, Luzhou, Sichuan 646000, P. R. China;

Corresponding author：

FengGuangfu, Email: 465197979@qq.com

Keywords：

Zipu gouty mixture; Orthogonal design; Extracting process; Chlorogenic acid

DOI：

10.7507/1002-0179.201600466

Video：

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Objective To optimize the extracting process of Zipu gouty mixture by orthogonal experiment, and to provide theoretical basis for its preparation procedure and quality control. Methods The water drawn extract yield and the total content of the chlorogenic acid were considered as research indexes. The orthogonal test was conducted to evaluate the effects of three factors including the amount of material/solvent ratio, extraction times, and duration of sample extraction of Zipu gouty mixture extracting process. Results The extracting process was optimized with the material/solvent ratio of 1:8, extract time of 30 minutes for 3 cycles. Conclusion The Zipu gouty mixture extracting process optimized by orthogonal test is simple, reliable and easy to repeat, which provides a theoretical basis for large-scale production.

Citation： LiuZhongjun, FengGuangfu, PiFengjuan, TuHe, ZhuLiyang, PangChaoping. Optimization of the Extracting Process of Zipu Gouty Mixture by Orthogonal Design. West China Medical Journal, 2016, 31(10): 1693-1696. doi: 10.7507/1002-0179.201600466 Copy

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1. Nan H, Qiao Q, Dong Y, et al. The prevalence of hyperuricemia in a population of the coastal city of Qingdao, China[J]. J Rheumatol, 2006, 33(7): 1346-1350.
2. 李昕雪.中药治疗痛风的系统综述和方法学研究[D].北京:北京中医药大学, 2013.
3. 左霞, 刘春霞, 杨金霞, 等. HPLC法测定不同来源不同采收期忍冬藤和金银花中绿原酸含量[J].中国药师, 2015(4): 682-684.
4. 熊茉君, 毛兴荣, 张敏, 等. HPLC测定柔肝解毒胶囊中的绿原酸[J].华西药学杂志, 2010, 25(6): 746-747.
5. 许沛虎, 高媛, 张雪琼, 等.正交实验法优选黄连生物碱提取工艺[J].医药导报, 2008, 27(4): 446-448.
6. 李根, 王桂蓉, 伍丽萍, 等.正交试验优选紫冰栓中紫草油提取工艺的研究[J].华西医学, 2010, 25(12): 2228-2230.
7. 史玉红.栀子药材中栀子苷和绿原酸含量测定方法研究[J].辽宁中医药大学学报, 2013, 15(6): 38-40.
8. 国家药典委员会.中华人民共和国药典-一部[M].北京:化学工业出版社, 2005: 286.
9. 潘新波, 李彩东, 王信, 等.正交试验法优选金茵利胆口服液水提工艺[J].中国药师, 2015, 18(4): 585-588.
10. 文卓琼, 蒋利华, 熊海蓉, 等.微波法提取石蒜中石蒜碱的工艺优化[J].中国农学通报, 2015, 31(24): 247-251.
11. 刘颖, 郭明晔, 白根本.绿原酸的研究进展[J].中药材, 2012, 35(7): 1180-1185.
12. 赵君峰, 白志川, 刘世尧.绿原酸提取及分析测定方法研究进展[J].辽宁中医药大学学报, 2007, 9(6): 80-82.
13. 余晟岚, 徐果.反相高效液相色谱法测定金嗓口服液中绿原酸含量[J].中国药业, 2015(10): 65-66.
14. 邓松岳, 王迎举. HPLC法测定抗毒合剂中绿原酸的含量[J].中国中医药现代远程教育, 2015, 13(9): 131-133.
15. 孟姝, 林玉莲, 都述虎. HPLC同时测定利胆片中绿原酸和黄芩苷的含量[J].中国实验方剂学杂志, 2015, 21(10): 77-79.

West China Medical Journal

Optimization of the Extracting Process of Zipu Gouty Mixture by Orthogonal Design

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