Effect of Anti-apoptosis Agent on Human Ovarian Tissue Novel Vitrification_West China Medical Journal

Authors：

 XiaoZhun , ZhangYaoyao ,  LiShangwei

Reproductive Medical Center, West China Second University Hospital, Sichuan University/Key Laboratory of Birth Defects and Retated Disease of Women and Children(Sichuan University), Minstry of Education, Chengdu, Sichuan 610041, P. R. China;

Corresponding author：

LiShangwei, Email: lishangwei2015@sina.com

Keywords：

Vitrification; Human ovarian tissue; Apoptosis; Cryoprotectant

DOI：

10.7507/1002-0179.201600502

Video：

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Objective To determine if the anti-apoptosis agent can improve the protective effect of human ovarian tissue with novel vitrification method. Methods Human ovarian cortical tissues were collected from ten patients treated between October 2012 and August 2013. The samples obtained from each patient were divided into two groups:control (the novel immersed vitrification) and experimental group (the novel immersed vitrification supplemented with vitamin C). The preservation effects in the two groups were compared by ultrastructure using electronic microscopy, and apoptosis was assessed by terminal deoxynucleotidyl transferase-medicated dUTP nick-end labeling (TUNEL) staining. Results The proportion of normal ultrastructure of oocytes and granulosa cells in the experimental group was higher than that in the control group (P<0.05). The proportion of TUNEL-positive primordial follicles in the experimental group was 21.6% (49/227) and the proportion of TUNEL-positive primordial follicles in the control group was 38.6% (91/236), with a significant difference between the two groups (P<0.001). The proportion of TUNEL-positive stromal cells in the experimental group was (21.33±3.41)% and the proportion of TUNEL-positive stromal cells in the control group was (33.46±3.09)%, also with a significant difference between the two groups (P<0.001). Conclusion Anti-apoptosis agents can improve the preservation of primordial follicles and stromal cells by inhibiting of apoptosis. It may be a method worthy of trying in order to improve the protective effect of human ovarian tissue vitrification.

Citation： XiaoZhun, ZhangYaoyao, LiShangwei. Effect of Anti-apoptosis Agent on Human Ovarian Tissue Novel Vitrification. West China Medical Journal, 2016, 31(11): 1829-1832. doi: 10.7507/1002-0179.201600502 Copy

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15.	肖准, 张曜曜, 李灵玲, 等. 人卵巢组织慢速冷冻保护液中添加抗凋亡剂研究[J].华西医学, 2016, 31(3):507-510.

1. Stoop D, Cobo A, Silber S. Fertility preservation for age-related fertility decline[J].Lancet, 2014, 384(9950):1311-1319.
2. Poirot C, Abirached F, Prades M, et al. Induction of puberty by autograft of cryopreserved ovarian tissue[J].Lancet, 2012, 379(9815):588.
3. De Vos M, Smitz J, Woodruff TK, et al. Fertility preservation in women with cancer[J].Lancet, 2014, 384(9950):1302-1310.
4. Donnez J, Dolmans MM, Demylle D, et al. Livebirth after orthotopic transplantation of cryopreserved ovarian tissue[J].Lancet, 2004, 364(9443):1405-1410.
5. Xiao Z, Wang Y, Li L, et al. Needle immersed vitrification can lower the concentration of cryoprotectant in human ovarian tissue cryopreservation[J].Fertil Steril, 2010, 94(6):2323-2328.
6. Xiao Z, Li SW, Zhang YY, et al. NIV versus Dropping vitrification in cryopreservation of human ovarian tissue[J].Cryo letters, 2014, 35(3):226-231.
7. Wang Y, Xiao Z, Li L, et al. Novel needle immersed vitrification:a practical and convenient method with potential advantages in mouse and human ovarian tissue cryopreservation[J].Hum Reprod, 2008, 23(10):2256-2265.
8. Raffaella D, Luigi N, Giuseppe L, et al. Evidence of apoptosis in human primordial and primary follicles[J].Hum Reprod, 2003, 18(12):2678-2682.
9. Kim SS, Yang HW, Kang HG, et al. Quantitative assessment of ischemic tissue damage in ovarian cortical tissue with or without antioxidant (ascorbic acid) treatment[J].Fertil Steril, 2004, 82(3):679-685.
10. Xiao Z, Yan W, Li NL, et al. In vitro culture thawed human ovarian tissue:NIV versus slow freezing method[J].Cryo letters, 2013, 34(5):520-526.
11. 肖准, 汪燕, 李灵玲, 等. 新型玻璃化冷冻法对人卵巢组织超微结构保存的研究[J].华西医学, 2013, 28(4):567-672.
12. 肖准, 汪燕, 李灵玲, 等. 新型玻璃化冷冻法在人卵巢组织深低温保存的应用[J].实用妇产科杂志, 2013, 29(12):63-67.
13. Keros V, Xella S, Hultenby K, et al. Vitrification versus controlled-rate freezing in cryopreservation of human ovarian tissue[J].Hum Reprod, 2009, 24(7):1670-1683.
14. Abir R, Fisch B, Jessel S, et al. Improving posttransplantation survival of human ovarian tissue by treating the host and graft[J].Fertil Steril, 2011, 95(4):1205-1210.
15. 肖准, 张曜曜, 李灵玲, 等. 人卵巢组织慢速冷冻保护液中添加抗凋亡剂研究[J].华西医学, 2016, 31(3):507-510.

West China Medical Journal

Effect of Anti-apoptosis Agent on Human Ovarian Tissue Novel Vitrification

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