干细胞治疗卵巢功能低下性不孕的研究进展和挑战_West China Medical Journal

Authors：

 郭清民 , 王慧 ,  郑艾

四川大学华西第二医院妇产科/出生缺陷与相关妇儿疾病教育部重点实验室（成都 610041）;

Corresponding author：

郑艾, Email: 894591422@qq.com

Keywords：

细胞治疗; 转分化; 雌性生殖细胞; 不孕，卵巢功能低下

DOI：

10.7507/1002-0179.201600535

Video：

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不孕不育发病率在世界范围内逐年增加，其中女性不孕患者50%～60%，其可能与生活环境及生活方式的改变有关。由于妇科恶性肿瘤发生率有逐年增加及年轻化趋势，卵巢功能缺陷或卵巢结构缺如导致生殖细胞缺陷的患者可能终身不孕，但干细胞移植治疗为这类患者带来了新的希望。干细胞是具有自我更新及多向分化潜能的未分化细胞，存在于胚胎、胎儿或成年各阶段，这些特点使其成为细胞治疗及再生医学领域的研究热点。理论上，在适宜条件下，干细胞能分化为包括雌性生殖细胞在内的三胚层不同类型细胞，进而形成机体不同组织及器官。现今，多能干细胞诱导分化为雌性生殖细胞的实验研究取得了一定的成果，因此，干细胞转分化为雌性生殖细胞从而进行细胞移植治疗及卵巢组织再生可能作为未来治疗不孕不育的新方法。此综述旨在总结干细胞向雌性生殖细胞转分化的研究进展及其应用于生殖医学所面临的挑战。

Citation： 郭清民, 王慧, 郑艾. 干细胞治疗卵巢功能低下性不孕的研究进展和挑战. West China Medical Journal, 2016, 31(11): 1949-1952. doi: 10.7507/1002-0179.201600535 Copy

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1. Boivin J, Bunting L, Collins JA, et al. International estimates of infertility prevalence and treatment-seeking:potential need and demand for infertility medical care[J].Hum Reprod, 2007, 22(6):1506-1512.
2. Schlegel PN. Evaluation of male infertility[J].Minerva Ginecol, 2009, 61(4):261-283.
3. Hübner K, Fuhrmann G, Christenson LK, et al. Derivation of oocytes from mouse embryonic stem cells[J].Science, 2003, 300(5623):1251-1256.
4. Volarevic V, Ljujic B, Stojkovic P, et al. Human stem cell research and regenerative medicine--present and future[J].Br Med Bull, 2011, 99(1):155-168.
5. Ratajczak MZ, Zuba-Surma E, Kucia M, et al. Pluripotent and multipotent stem cells in adult tissues[J].Adv Med Sci, 2012, 57(1):1-17.
6. Takahashi K, Yamanaka S. Induction of pluripotent stem cells from mouse embryonic and adult fibroblast cultures by defined factors[J].Cell, 2006, 126(4):663-676.
7. Deuse T, Wang D, Stubbendorff M, et al. SCNT-derived ESCs with mismatched mitochondria trigger an immune response in allogeneic hosts[J].Cell Stem Cell, 2014, 16(1):33-38.
8. Ko K, Huebner K, Mueller-Keuker J, et al. In vitro derivation of germ cells from embryonic stem cells[J].Front Biosci (Landmark Ed), 2010, 15(1):46-56.
9. Clark AT, Bodnar MS, Fox M, et al. Spontaneous differentiation of germ cells from human embryonic stem cells in vitro[J].Hum Mol Genet, 2004, 13(7):727-739.
10. Tada M, Takahama Y, Abe K, et al. Nuclear reprogramming of somatic cells by in vitro hybridization with ES cells[J].Curr Biol, 2001, 11(19):1553-1558.
11. Abeyta MJ, Clark AT, Rodriguez RT, et al. Unique gene expression signatures of independently-derived human embryonic stem cell lines[J].Hum Mol Genet, 2004, 13(6):601-608.
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13. Johnson J, Canning J, Kaneko T, et al. Germline stem cells and follicular renewal in the postnatal mammalian ovary[J].Nature, 2004, 428(6979):145-150.
14. Bai Y, Yu M, Hu Y, et al. Location and characterization of female germline stem cells (FGSCs) in juvenile porcine ovary[J].Cell Prolif, 2013, 46(5):516-528.
15. Zou K, Yuan Z, Yang Z, et al. Production of offspring from a germline stem cell line derived from neonatal ovaries[J].Nat Cell Biol, 2009, 11(5):631-636.
16. Dyce PW, Wen L, Li J. In vitro germline potential of stem cells derived from fetal porcine skin[J].Nat Cell Biol, 2006, 8(4):384-390.
17. Dyce PW, Liu J, Tayade C, et al. In vitro and in vivo germ line potential of stem cells derived from newborn mouse skin[J].PLoS One, 2011, 6(5):143-149.
18. Virant-Klun I, Skutella T, Kubista M, et al. Expression of pluripotency and oocyte-related genes in single putative stem cells from human adult ovarian surface epithelium cultured in vitro in the presence of follicular fluid[J].Biomed Res Int, 2013(7):861460.
19. Anchan R, Gerami-Naini B, Lindsey JS, et al. Efficient differentiation of steroidogenic and germ-like cells from epigenetically-related iPSCs derived from ovarian granulosa cells[J].PLoS One, 2015, 10(3):e0119275.
20. Eguizabal C, Montserrat N, Vassena R, et al. Complete meiosis from human induced pluripotent stem cells[J].Stem Cells, 2011, 29(8):1186-1195.
21. Goud PT, Goud AP, Qian C, et al. In-vitro maturation of human germinal vesicle stage oocytes:role of cumulus cells and epidermal growth factor in the culture medium[J].Human Reprod, 1998, 13(6):1638-1644.
22. Hayashi K, Ogushi S, Kurimoto K, et al. Offspring from oocytes derived from in vitro primordial germ cell-like cells in mice[J].Science, 2012, 338(619):971-975.
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25. Yong E. Ovarian stem cell debate[J].Scientist, 2012, 26:26.

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干细胞治疗卵巢功能低下性不孕的研究进展和挑战

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