Epilepsy model rat hippocampus dentate gyrus neurons apoptosis and the significance of apoptosis-inducing factor and Caspase-3 joint expression_West China Medical Journal

Authors：

DING Chunxiao ¹ , LIU Xiaohu ¹ , QI Yue ¹ , QIAO Jujiu ¹ ,  LI Jianhua ²

1. Department of Pathology, Chinese Medicine Research Institute of Liaoning Province, Shenyang, Liaoning 110034, P. R. China;
2. Division and Department of Pathology, the First Affiliated Hospital, Chinses Medical University, Shenyang, Liaoning 110001, P. R. China;

Corresponding author：

LI Jianhua, Email: LIJH@mail.cmu.edu.cn

Keywords：

Pentrolone; Apoptosis; Nissl staining; Immunohistochemistry; Caspase-3; Apoptosis-inducing factor; Epilepsy

DOI：

10.7507/1002-0179.201604034

Video：

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Objective To observe the apoptosis of hippocampal dentate gyrus in rats with pentrolone model of epilepsy, and detect the significance of apoptosis-inducing factor (AIF) and Caspase-3 joint expression. Methods Twelve Wistar rats were randomly divided into model group and control group with 6 in each. Intraperitoneal injection of pentrolone was carried out for rats in the model group, while physiological saline was injected in rats of the control group. After the model was successfully established, we observed the Nissl staining results of hippocampus dentate gyrus tissue in the rats as well as the expression of AIF and Caspase-3 protein in both groups. Results The single visual nerve cell counts of the control group and the model group under 400-times magnification were respectively 99.76±11.89 and 78.69±10.94; Caspase-3 gray values of the two groups were respectively 154.81±16.06 and 131.65±16.81; and AIF protein gray values were respectively 173.09±9.57 and 158.34±6.33. All the above differences were statistically significant (P<0.05). Conclusion The two apoptotic pathways through Caspase-3 dependent on Caspase and AIF independent of Caspase are both involved in the neuronal apoptosis induced by epilepsy.

Citation： DING Chunxiao, LIU Xiaohu, QI Yue, QIAO Jujiu, LI Jianhua. Epilepsy model rat hippocampus dentate gyrus neurons apoptosis and the significance of apoptosis-inducing factor and Caspase-3 joint expression. West China Medical Journal, 2017, 32(4): 550-553. doi: 10.7507/1002-0179.201604034 Copy

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11.	冯建利, 姚博, 闫鹏, 等. 大鼠局灶性脑缺血再灌注后凋亡诱导因子的表达及其与神经细胞凋亡的关系. 中国老年学杂志, 2007, 27(20): 1955-1957.
12.	李巧云, 张海华, 游仁芳. Caspase-3 表达及神经元凋亡的影响. 南昌大学学报, 2013, 53(10): 11-14.
13.	高维娟, 张霞, 刘莎莎. 大鼠脑缺血再灌注后海马 CA1 区 Caspase-3 的表达及细胞凋亡的动态变化. 中国老年学杂志, 2013, 33(24): 6202-6204.
14.	杨宜承, 赵薇, 曾常茜, 等. 雷公藤内酯对海人酸致痫大鼠神经元 caspase3 和 caspase9 蛋白表达的影响. 辽宁中医药大学学报, 2013, 15(2): 48-50.
15.	刘小虎, 向邵杰, 齐越, 等. 戊四唑急性癫痫模型海马病理组织的病理变化. 中国药理学通报, 2015, 31(4): 514-518.
16.	Nicholson DW, Ali A, Thornberry NA, et al. Identification and inhibition of the ICE/CED-3 protease necessary for mammalian apoptosis. Nature, 1995, 376(6535): 37-43.
17.	Izzi L, Attisano L. Ubiquitin-dependent regulation of TGF beta signaling in cancer. Neoplasia, 2006, 8(8): 677-688.

1. Schubert M, Siegmund H, Pape HC, et al. Kindling-induced changes in plasticity of the rat amygdala and hippocampus. Learn Mem, 2005, 12(5): 520-526.
2. 秦晓云, 窦长武, 张占普, 等. 动态脑电图监测癫痫患者的临床意义. 西部医学, 2016, 28(3): 358-360, 364.
3. 杨帆, 杨永清. 癫痫与脑神经元损伤. 医学综述, 2009, 15(19): 2881-2884.
4. 李应宏, 莫烨, 李劲梅, 等. 耐药性癫痫患者脑内 Nurr1 基因及蛋白产物的表达. 西部医学, 2015, 27(1): 5-8, 12.
5. 王守权, 隋鸿锦, 宫瑾, 等. 戊四氮急性癫痫模型齿状回分子层突触的可塑性变化. 解剖学杂志, 2004, 27(3): 288-290.
6. 王玢, 迟华基, 袁方曜. 中药复方对戊四氮致痫大鼠海马神经元 caspase-3 表达的影响. 山东教育学院学报, 2010, 25(6): 39-42.
7. 梁义, 杨忠, 魏璐, 等. 戊四氮点燃 SD 大鼠制作慢性癫痫模型中不同注射剂量的对比研究. 武警医学, 2007, 18(4): 264-266.
8. Shibeeb O, Wood JP, Casson RJ, et al. Effects of a conventional photocoagulator and a 3-ns pulse laser on preconditioning responses and retinal ganglion cell survival after optic nerve crush. Exp Eye Res, 2014, 127(127): 77-90.
9. Dhaher R, Damisah EC, Wang H, et al. 5-aminovaleric acid suppresses the development of severe seizures in the methionine sulfoximine model of mesial temporal lobe epilepsy. Neurobiol Dis, 2014, 67(67): 18-23.
10. 张玮, 韩宁, 胡金凤, 等. 化合物 IMM-H004 对大鼠急性全脑缺血/再灌注损伤的保护作用. 中国药理学通报, 2013, 29(12): 1654-1659.
11. 冯建利, 姚博, 闫鹏, 等. 大鼠局灶性脑缺血再灌注后凋亡诱导因子的表达及其与神经细胞凋亡的关系. 中国老年学杂志, 2007, 27(20): 1955-1957.
12. 李巧云, 张海华, 游仁芳. Caspase-3 表达及神经元凋亡的影响. 南昌大学学报, 2013, 53(10): 11-14.
13. 高维娟, 张霞, 刘莎莎. 大鼠脑缺血再灌注后海马 CA1 区 Caspase-3 的表达及细胞凋亡的动态变化. 中国老年学杂志, 2013, 33(24): 6202-6204.
14. 杨宜承, 赵薇, 曾常茜, 等. 雷公藤内酯对海人酸致痫大鼠神经元 caspase3 和 caspase9 蛋白表达的影响. 辽宁中医药大学学报, 2013, 15(2): 48-50.
15. 刘小虎, 向邵杰, 齐越, 等. 戊四唑急性癫痫模型海马病理组织的病理变化. 中国药理学通报, 2015, 31(4): 514-518.
16. Nicholson DW, Ali A, Thornberry NA, et al. Identification and inhibition of the ICE/CED-3 protease necessary for mammalian apoptosis. Nature, 1995, 376(6535): 37-43.
17. Izzi L, Attisano L. Ubiquitin-dependent regulation of TGF beta signaling in cancer. Neoplasia, 2006, 8(8): 677-688.

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