循环肿瘤细胞检测及 SE-iFISH 技术检测循环肿瘤细胞的临床应用_West China Medical Journal

Authors：

张静 ¹ , 蒋婷婷 ² ,  陈念永 ²

1. ;
2. ;

Corresponding author：

陈念永, Email: nchenyy@yahoo.com

Keywords：

循环肿瘤细胞; 恶性肿瘤; SE-iFISH 技术; 差相富集; 临床应用

DOI：

10.7507/1002-0179.201607140

Video：

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Abstract Full text Figures/Tables Video References Cited by

循环肿瘤细胞（circulating tumor cells，CTC）是指从实体肿瘤原发或转移病灶脱落并侵入外周血循环的肿瘤细胞，是恶性肿瘤远处转移的主要原因。目前 CTC 检测技术发展迅速，但由于外周血中 CTC 个数稀少且存在较大的异质性，限制了许多检测技术的应用。SE-iFISH 技术将差相富集、瘤标免疫荧光染色与染色体荧光原位杂交（immunostaining-fluorescence in situ hybridization，i-FISH）结合，不依赖肿瘤上皮细胞表面标示物的表达，对 CTC 同时进行瘤标染色与 i-FISH 染色体计数的双重检测，具有高灵敏性和高特异性。现就 CTC 检测方法及 SE-iFISH 技术检测 CTC 的临床应用作一综述。

Citation： 张静, 蒋婷婷, 陈念永. 循环肿瘤细胞检测及 SE-iFISH 技术检测循环肿瘤细胞的临床应用. West China Medical Journal, 2017, 32(7): 1100-1103. doi: 10.7507/1002-0179.201607140 Copy

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17.	Li Y, Zhang X, Ge S, et al. Clinical significance of phenotyping and karyotyping of circulating tumor cells in patients with advanced gastric cancer. Oncotarget, 2014, 5(16): 6594-6602.
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26.	Jiang J, Wang DD, Yang M, et al. Comprehensive characterization of chemotherapeutic efficacy on metastases in the established gastric neuroendocrine cancer patient derived xenograft model. Oncotarget, 2015, 6(17): 15639-15651.

1. Paterlini-Brechot P, Benali NL. Circulating tumor cells (CTC) detection: clinical impact and future directions. Cancer Lett, 2007, 253(2): 180-204.
2. Ashworth T. A case of cancer in which cells similar to those in the tumours were seen in the blood after death. Med J Aust, 1869, 14: 146-149.
3. Pierga JY, Bonneton C, Vincent-Salomon A, et al. Clinical significance of immunocytochemical detection of tumor cells using digital microscopy in peripheral blood and bone marrow of breast cancer patients. Clin Cancer Res, 2004, 10(4): 1392-1400.
4. Cristofanilli M. Circulating tumor cells, disease progression, and survival in metastatic breast cancer. Semin Oncol, 2006, 33, Supplement 9 (8): 9-14.
5. Sastre J, Maestro ML, Puente J, et al. Circulating tumor cells in colorectal cancer: correlation with clinical and pathological variables. Ann Onco, 2008, 19(5): 935-938.
6. Okabe H, Tsunoda S, Hosogi H, et al. Circulating tumor cells as an independent predictor of survival in advanced gastric cancer. Ann Surg Oncol, 2015, 22(12): 3954-3961.
7. Bono JS, Scher HI, Montgomery RB, et al. Circulating tumor cells predict survival benefit from treatment in metastatic castrationresistant prostate cancer. Clin Cancer Res, 2008, 14(19): 6302-6309.
8. Krebs MG, Sloane R, Priest L, et al. Evaluation and prognostic significance of circulating tumor cells in patients with non-small-cell lung cancer. J Clin Oncol, 2011, 29(12): 1556-1563.
9. Harris L, Fritsche H, Mennel R, et al. American society of clinical oncology 2007 update of recommendations for the use of tumor markers in breast cancer. J Clin Oncol, 2007, 25(33): 5287-5312.
10. Alix-Panabières C, Pantel K. Circulating tumor cells: liquid biopsy of cancer. Clin Chem, 2013, 59(1): 110-118.
11. Pantel K, Alix-Panabières C. Real-time liquid biopsy in cancer patients: fact or fiction? Cancer Res, 2013, 73(21): 6384-6388.
12. Alix-Panabières C, Pantel K. Challenges in circulating tumour cell research. Nat Rev Cancer, 2014, 14(9): 623-631.
13. Lin PP. Integrated EpCAM-independent subtraction enrichment and iFISH strategies to detect and classify disseminated and circulating tumors cells. Clin Transl Med, 2015, 4(1): 38.
14. Harouaka R, Kang Z, Zheng SY, et al. Circulating tumor cells: advances in isolation and analysis, and challenges for clinical applications. Pharmacol Ther, 2014, 141(2): 209-221.
15. Miller MC, Doyle GV, Terstappen LW. Significance of circulating tumor cells detected by the CellSearch system in patients with metastatic breast colorectal and prostate cancer. J Oncol, 2010, 2010: 617421.
16. Gires O, Stoecklein NH. Dynamic EpCAM expression on circulating and disseminating tumor cells: causes and Consequences. Cell Mol Life Sci, 2014, 71(22): 4393-4402.
17. Li Y, Zhang X, Ge S, et al. Clinical significance of phenotyping and karyotyping of circulating tumor cells in patients with advanced gastric cancer. Oncotarget, 2014, 5(16): 6594-6602.
18. Ge F, Zhang H, Wang DD, et al. Enhanced detection and comprehensive in situ phenotypic characterization of circulating and disseminated heteroploid epithelial and glioma tumor cells. Oncotarget, 2015, 6(29): 27049-27064.
19. Weitz J, Kienle P, Lacroix J, et al. Dissemination of tumor cells in patients undergoing surgery for colorectal cancer. Clin Cancer Res, 1998, 4(2): 343-348.
20. Hong B, Zu Y. Detecting circulating tumor cells: current challenges and new trends. Theranostics, 2013, 3(6): 377-394.
21. Yu M, Stott S, Toner M, et al. Circulating tumor cells: approaches to isolation and characterization. J Cell Biol, 2011, 192(3): 373-382.
22. Gasparini P, Sozzi G, Pierotti MA. The role of chromosomal alterations in human cancer development. J Cell Biochem, 2007, 102(2): 320-331.
23. Gao Y, Zhu Y, Zhang Z, et al. Clinical significance of pancreatic circulating tumor cells using combined negative enrichment and immunostaining-fluorescence in situ hybridization. J Exp Clin Cancer Res, 2016, 35: 66.
24. Cytelligen. 赛特生物专注于循环肿瘤细胞与转化医学研究. (2016-03-21)[2017-06-27]. http://www.cytointelligen. com/technology.html.
25. Bidard FC, Huguet F, Louvet C, et al. Circulating tumor cells in locally advanced pancreatic adenocarcinoma: the ancillary Circe 07 study to the LAP 07 trial. Ann Onco, 2013, 24(8): 2057-2061.
26. Jiang J, Wang DD, Yang M, et al. Comprehensive characterization of chemotherapeutic efficacy on metastases in the established gastric neuroendocrine cancer patient derived xenograft model. Oncotarget, 2015, 6(17): 15639-15651.

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West China Medical Journal

循环肿瘤细胞检测及 SE-iFISH 技术检测循环肿瘤细胞的临床应用

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