Comparison of diagnostic significance between myeloperoxidase staining and cytoplasm myeloperoxidase in acute leukemia cells_West China Medical Journal

Authors：

CHEN Jiao , JIN Yongmei , ZHENG Qin , SHI Qing , CHEN Xingyuan ,  SU Jun

Department of Laboratory Medicine, West China Hospital, Sichuan University, Chengdu, Sichuan 610041, P. R. China;

Corresponding author：

SU Jun, Email: sjhauxi1667@163.com

Keywords：

Acute leukemia; Myeloperoxidase staining; Cytoplasm myeloperoxidase flow cytometry

DOI：

10.7507/1002-0179.201609066

Video：

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Objective To detect the difference between the peroxidase (POX) by cytochemical staining and cytoplasm myeloperoxidase (cMPO) by flow cytometry in acute leukemia cells, and provide a more accurate basis for the classification of leukemia. Methods The positive rate of POX in acute leukemia cells was detected by cytochemical staining. The positive rate of cMPO in acute leukemia cells was detected by flow cytometry. Then the positive rate of POX and cMPO, and the positive cells score were analyzed. Results The positive rate and the positive cells scores between POX and cMPO in acute lymphoblastic leukemia were significantly different (P<0.05), the positive rate and the positive cells scores of POX were significantly higher than those of cMPO. The positive rate between POX and cMPO in acute non-lymphoblastic leukemia (ANLL) had significant differences (P<0.05), the positive rate of cMPO was higher than that of POX; but no difference was found between POX and cMPO positive cells scores in ANLL (P>0.05). In acute myelocytic leukemia (AML)-M1 subtype, significant difference was found in the positive rate between POX and cMPO (P=0.006); cMPO positive rate was significantly higher than that of POX, but the POX positive cells score was significantly higher than that of cMPO (P=0.001). There were no significances of positive rate and positive cells score in AML-M2, AML-M3, AML-M4, AML-M5 subtypes between POX and cMPO (P>0.05). Conclusions There are not major differences between positive rate of POX and cMPO, as well as the positive cells scores in acute leukemia, especially acute myelocytic leukemia. We can choose the better method according to the actual situation and the sensitivity requirements. The two methods should be replenished by each other and used alternately.

Citation： CHEN Jiao, JIN Yongmei, ZHENG Qin, SHI Qing, CHEN Xingyuan, SU Jun. Comparison of diagnostic significance between myeloperoxidase staining and cytoplasm myeloperoxidase in acute leukemia cells. West China Medical Journal, 2017, 32(7): 1019-1023. doi: 10.7507/1002-0179.201609066 Copy

1.	Khwaja A, Bjorkholm M, Gale RE, et al. Acute myeloid leukaemia. Nat Rev Dis Primers, 2016, 10(2): 16010.
2.	Guy J, Antony-Debré I, Benayoun E, et al. Flow cytometry thresholds of myeloperoxidase detection to discriminate between acute lymphoblastic or myeloblastic leukaemia. Br J Haematol, 2013, 161(4): 551-555.
3.	Chen WX, Zhu HL, Xue M, et al. Quick staining technique for myeloperoxidase using potassium iodide and oxidized pyronine B. Acta Histochem, 2014, 116(1): 292-296.
4.	Lee D, Grigoriadis G, Westerman D. The role of multiparametric flow cytometry in the detection of minimal residual disease in acute leukaemia. Pathology, 2015, 47(7): 609-621.
5.	Sukumaran R, Nair RA, Jacob PM, et al. Flow cytometric analysis of mixed phenotype acute leukemia: experience from a tertiary oncology center. Indian J Pathol Microbiol, 2015, 58(2): 181-186.
6.	刘想想, 熊北斗, 王敏, 等. 髓过氧化物酶基因多态性与白血病关系的 Meta 分析. 公共卫生与预防医学, 2016, 27(2): 5-7.
7.	Döhner H, Weisdorf DJ, Bloomfield CD. Acute myeloid leukemia. N Engl J Med, 2015, 373(12): 1136-1152.
8.	胡彩虹, 杨桂斌, 张夏, 等. MICM分型在急性早幼粒细胞白血病诊断中的应用. 国际检验医学, 2013, 34(24): 3347-3348.
9.	McKenna RW, Parkin J, Bloomfield CD, et al. Acute promyelocyticleukaemia: a study of 39 cases with identification of a hyperbasophilicmicrogranular variant. Br J Haematol, 1982, 50(2): 201-214.
10.	Latger-Cannard V, Bardet V, Malet M, et al. Evaluation of peroxidase activity by alpha-naphthol/pyronine staining compared with benzidine staining in 101 acute leukemia cases. Lab Hematol, 2010, 16(4): 76-82.
11.	常军林, 巨小英. 髓过氧化物酶染色对急性白血病分型诊断的价值. 吉林医学, 2014, 22: 4925-4926.
12.	Zhou JZ. Positive rate and score discrepancies of myeloperoxidase staining at different temperatures. Acta Histochem, 2014, 116(1): 289-291.
13.	Sharma A, Buxi G, Walia R, et al. Childhood acute erythroleukemia diagnosis by flow cytometry. Indian J Pathol Microbiol, 2011, 54(1): 173-175.
14.	Ikoma MR, Beltrame MP, Ferreira S, et al. Proposal for the standardization of flowcytometry protocols to detect minimal residual disease in acutelymphoblastic leukemia. Rev Bras Hematol Hemoter, 2015, 37(6): 406-413.
15.	Saravanan L, Juneja S. Immunohistochemistry is a more sensitive marker for the detection of myeloperoxidase in acute myeloid leukemia compared with flow cytometry and cytochemistry. Int J Lab Hematol, 2010, 32(1,1): E132-E136.
16.	Manivannan P, Puri V, Somasundaram V, et al. Can threshold for MPO by flow cytometry be reduced in classifying acute leukaemia? A comparison of flowcytometric and cytochemical myeloperoxidase using different flow cytometric cut-offs. Hematology, 2015, 20(8): 455-461.

1. Khwaja A, Bjorkholm M, Gale RE, et al. Acute myeloid leukaemia. Nat Rev Dis Primers, 2016, 10(2): 16010.
2. Guy J, Antony-Debré I, Benayoun E, et al. Flow cytometry thresholds of myeloperoxidase detection to discriminate between acute lymphoblastic or myeloblastic leukaemia. Br J Haematol, 2013, 161(4): 551-555.
3. Chen WX, Zhu HL, Xue M, et al. Quick staining technique for myeloperoxidase using potassium iodide and oxidized pyronine B. Acta Histochem, 2014, 116(1): 292-296.
4. Lee D, Grigoriadis G, Westerman D. The role of multiparametric flow cytometry in the detection of minimal residual disease in acute leukaemia. Pathology, 2015, 47(7): 609-621.
5. Sukumaran R, Nair RA, Jacob PM, et al. Flow cytometric analysis of mixed phenotype acute leukemia: experience from a tertiary oncology center. Indian J Pathol Microbiol, 2015, 58(2): 181-186.
6. 刘想想, 熊北斗, 王敏, 等. 髓过氧化物酶基因多态性与白血病关系的 Meta 分析. 公共卫生与预防医学, 2016, 27(2): 5-7.
7. Döhner H, Weisdorf DJ, Bloomfield CD. Acute myeloid leukemia. N Engl J Med, 2015, 373(12): 1136-1152.
8. 胡彩虹, 杨桂斌, 张夏, 等. MICM分型在急性早幼粒细胞白血病诊断中的应用. 国际检验医学, 2013, 34(24): 3347-3348.
9. McKenna RW, Parkin J, Bloomfield CD, et al. Acute promyelocyticleukaemia: a study of 39 cases with identification of a hyperbasophilicmicrogranular variant. Br J Haematol, 1982, 50(2): 201-214.
10. Latger-Cannard V, Bardet V, Malet M, et al. Evaluation of peroxidase activity by alpha-naphthol/pyronine staining compared with benzidine staining in 101 acute leukemia cases. Lab Hematol, 2010, 16(4): 76-82.
11. 常军林, 巨小英. 髓过氧化物酶染色对急性白血病分型诊断的价值. 吉林医学, 2014, 22: 4925-4926.
12. Zhou JZ. Positive rate and score discrepancies of myeloperoxidase staining at different temperatures. Acta Histochem, 2014, 116(1): 289-291.
13. Sharma A, Buxi G, Walia R, et al. Childhood acute erythroleukemia diagnosis by flow cytometry. Indian J Pathol Microbiol, 2011, 54(1): 173-175.
14. Ikoma MR, Beltrame MP, Ferreira S, et al. Proposal for the standardization of flowcytometry protocols to detect minimal residual disease in acutelymphoblastic leukemia. Rev Bras Hematol Hemoter, 2015, 37(6): 406-413.
15. Saravanan L, Juneja S. Immunohistochemistry is a more sensitive marker for the detection of myeloperoxidase in acute myeloid leukemia compared with flow cytometry and cytochemistry. Int J Lab Hematol, 2010, 32(1,1): E132-E136.
16. Manivannan P, Puri V, Somasundaram V, et al. Can threshold for MPO by flow cytometry be reduced in classifying acute leukaemia? A comparison of flowcytometric and cytochemical myeloperoxidase using different flow cytometric cut-offs. Hematology, 2015, 20(8): 455-461.

West China Medical Journal

Comparison of diagnostic significance between myeloperoxidase staining and cytoplasm myeloperoxidase in acute leukemia cells

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