Interference of antibiotic use on detection of <i>Helicobacter pylori</i> in children _West China Medical Journal

Authors：

XIE Yongmei , WANG Zhiling , WANG Liyuan , CAI Xiaotang ,  GAO Shan

Department of Pediatric, West China Second University Hospital, Sichuan University; Key Laboratory of Birth Defects and Related Diseases of Women and Children (Sichuan University) Ministry of Education; Chengdu, Sichuan 610041, P. R. China;

Corresponding author：

GAO Shan, Email: huaxigaoshan@163.com

Keywords：

Helicobacter pylori; Antibiotic; Pediatrics

DOI：

10.7507/1002-0179.201806126

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Objective To explore the interference of recent use of antibiotics in the sensitivities of different methods for the detection of Helicobacter pylori (Hp) in children. Methods From June 2015 to December 2017, children who were highly suspected of Hp infection and required gastroscopy were divided into the antibiotic group and the control group according to the recent use of antibiotics, with 200 cases in each group. The use of antibiotics of children in the antibiotic group was analyzed. The children in the two groups completed five methods of Hp detection, including rapid urease test (RUT), ¹³C-urea breath test (¹³C-UBT), pathological Warthin-Starry staining, colloidal gold method for measuring Hp immunoglobulin M (IgM) antibody, and enzyme linked immunosorbent assay method for measuring Hp stool antigen (HpSA). Comparative analyses of data were done. Results The positive rate of RUT was 36.5% in the antibiotic group and 75.0% in the control group (P<0.05). The positive rate and value of¹³C-UBT were 59.0% and 13.6±4.7 in the antibiotic group, and 78.0% and 41.7±6.6 in the control group (P<0.05). The positive rate of pathological biopsy was 48.0% in the antibiotic group and 68.0% in the control group (P<0.05), and L-forms were found in 80.9% of the Hp-positive specimens in the antibiotic group. The positive rate of IgM antibody was 54.5% in the antibiotic group and 65.5% in the control group (P<0.05). The positive rate of HpSA was 38.0% in the antibiotic group and 69.0% in the control group (P<0.05). In the antibiotic group, only 12.5% of the children used antibiotics for eradication of Hp and 87.5% for non Hp eradication. Conclusions Under the interference of antibiotics use, the sensitivity of ¹³C-UBT is the highest among the five methods for Hp detection, and the sensitivities of RUT and HpSA are very low. For children who could not cooperate with ¹³C-UBT, the sensitivity of IgM detection is relatively high. For children who need gastroscopy, prolonged chromogenic time for RUT and multi-point pathological biopsy can reduce misdiagnosis.

Citation： XIE Yongmei, WANG Zhiling, WANG Liyuan, CAI Xiaotang, GAO Shan. Interference of antibiotic use on detection of Helicobacter pylori in children . West China Medical Journal, 2018, 33(12): 1513-1518. doi: 10.7507/1002-0179.201806126 Copy

1.	O’Ryan ML, Lucero Y, Rabello M, et al. Persistent and transient Helicobacter pylori infections in early childhood. Clin Infect Dis, 2015, 61(2): 211-218.
2.	Mentis A, Lehours P, Megraud F. Epidemiology and diagnosis of Helicobacter pylori infection. Helicobacter, 2015, 20(Suppl 1): 1-7.
3.	Sarem M, Corti R. Role of Helicobacter pylori coccoid forms in infection and recrudescence. Gastroenterol Hepatol, 2016, 39(1): 28-35.
4.	Narang M, Puri AS, Sachdeva S, et al. Celiac disease and Helicobacter pylori infection in children: is there any association?. J Gastroenterol Hepatol, 2017, 32(6): 1178-1182.
5.	Muhsen K, Goren S, Cohen D. Helicobacter pylori infection in early childhood and growth at school age. Helicobacter, 2015, 20(6): 410-417.
6.	中华医学会消化病学分会幽门螺杆菌和消化性溃疡学组, 全国幽门螺杆菌研究协作组, 刘文忠, 等. 第五次中国全国共识幽门螺杆菌感染管理报告. 中华内科杂志, 2017, 56(7): 532-545.
7.	Liu WZ, Xie Y, Lu H, et al. Fifth Chinese National Consensus Report on the management of Helicobacter pylori infection. Helicobacter, 2018, 23(2): e12475.
8.	Malfertheiner P, Megraud F, O’Morain CA, et al. Management of Helicobacter pylori infection-the Maastricht V/Florence Consensus Report. Gut, 2017, 66(1): 6-30.
9.	Sánchez-Cuén JA, Canizalez-Román VA, León-Sicairos NM, et al. Concordance among invasive diagnostic procedures for Helicobacter pylori infection in adults. Salud Publica Mex, 2015, 57(4): 352-357.
10.	Sugano K, Tack J, Kuipers EJ, et al. Kyoto global consensus report on Helicobacter pylori gastritis. Gut, 2015, 64(9): 1353-1367.
11.	Li L, Ke Y, Yu C, et al. Antibiotic resistance of Helicobacter pylori in Chinese children: a multicenter retrospective study over 7 years. Helicobacter, 2017, 22(3). https://doi.org/10.1111/hel.12373.
12.	Leja M, Axon A, Brenner H. Epidemiology of Helicobacter pylori infection. Helicobacter, 2016, 21(Suppl 1): 3-7.
13.	Chai FY, Chong HC, Tan YE, et al. Helicobacter pylori infection rates in patients undergoing endoscopy in the interior of borneo. Helicobacter, 2016, 21(2): 158-162.
14.	Dos Santos AA, Carvalho AA. Pharmacological therapy used in the elimination of Helicobacter pylori infection: a review. World J Gastroenterol, 2015, 21(1): 139-154.
15.	Saha R, Roy P, Das S, et al. Application of a stool antigen test to evaluate the burden of Helicobacter pylori infection in dyspepsia patients. Indian J Pathol Microbiol, 2016, 59(1): 66-68.

1. O’Ryan ML, Lucero Y, Rabello M, et al. Persistent and transient Helicobacter pylori infections in early childhood. Clin Infect Dis, 2015, 61(2): 211-218.
2. Mentis A, Lehours P, Megraud F. Epidemiology and diagnosis of Helicobacter pylori infection. Helicobacter, 2015, 20(Suppl 1): 1-7.
3. Sarem M, Corti R. Role of Helicobacter pylori coccoid forms in infection and recrudescence. Gastroenterol Hepatol, 2016, 39(1): 28-35.
4. Narang M, Puri AS, Sachdeva S, et al. Celiac disease and Helicobacter pylori infection in children: is there any association?. J Gastroenterol Hepatol, 2017, 32(6): 1178-1182.
5. Muhsen K, Goren S, Cohen D. Helicobacter pylori infection in early childhood and growth at school age. Helicobacter, 2015, 20(6): 410-417.
6. 中华医学会消化病学分会幽门螺杆菌和消化性溃疡学组, 全国幽门螺杆菌研究协作组, 刘文忠, 等. 第五次中国全国共识幽门螺杆菌感染管理报告. 中华内科杂志, 2017, 56(7): 532-545.
7. Liu WZ, Xie Y, Lu H, et al. Fifth Chinese National Consensus Report on the management of Helicobacter pylori infection. Helicobacter, 2018, 23(2): e12475.
8. Malfertheiner P, Megraud F, O’Morain CA, et al. Management of Helicobacter pylori infection-the Maastricht V/Florence Consensus Report. Gut, 2017, 66(1): 6-30.
9. Sánchez-Cuén JA, Canizalez-Román VA, León-Sicairos NM, et al. Concordance among invasive diagnostic procedures for Helicobacter pylori infection in adults. Salud Publica Mex, 2015, 57(4): 352-357.
10. Sugano K, Tack J, Kuipers EJ, et al. Kyoto global consensus report on Helicobacter pylori gastritis. Gut, 2015, 64(9): 1353-1367.
11. Li L, Ke Y, Yu C, et al. Antibiotic resistance of Helicobacter pylori in Chinese children: a multicenter retrospective study over 7 years. Helicobacter, 2017, 22(3). https://doi.org/10.1111/hel.12373.
12. Leja M, Axon A, Brenner H. Epidemiology of Helicobacter pylori infection. Helicobacter, 2016, 21(Suppl 1): 3-7.
13. Chai FY, Chong HC, Tan YE, et al. Helicobacter pylori infection rates in patients undergoing endoscopy in the interior of borneo. Helicobacter, 2016, 21(2): 158-162.
14. Dos Santos AA, Carvalho AA. Pharmacological therapy used in the elimination of Helicobacter pylori infection: a review. World J Gastroenterol, 2015, 21(1): 139-154.
15. Saha R, Roy P, Das S, et al. Application of a stool antigen test to evaluate the burden of Helicobacter pylori infection in dyspepsia patients. Indian J Pathol Microbiol, 2016, 59(1): 66-68.

West China Medical Journal

Interference of antibiotic use on detection of Helicobacter pylori in children

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