Study on the positive rates of several virulence genes in 171 clinical strains of <i>Streptococcus pneumoniae</i>_West China Medical Journal

Authors：

GAO Bing , ZHANG Hongbo , ZHANG Chi ,  PENG Zheng

Department of Clinical Laboratory, Tongji Hospital Affiliated to Tongji Medical College of Huazhong University of Science and Technology, Wuhan, Hubei 430030, P. R. China 430030;

Corresponding author：

PENG Zheng, Email: pengzheng2013@126.com

Keywords：

Streptococcus pneumoniae; Virulence gene; Septicemia; Pneumonia

DOI：

10.7507/1002-0179.201906078

Video：

Export PDF Favorites Scan Get Citation

Abstract Full text Figures/Tables Video References Cited by

Objective To investigate the positive rates of virulence genes ply, lytA and nanA in Streptococcus pneumoniae (SP) strains isolated from different sources and the pathogenesis.Methods A total of 147 and 24 strains of SP were isolated from sputum and blood samples of hospitalized children in Tongji Hospital of Wuhan from 2015 to 2016, respectively. Such strains of SP were analyzed by automated microbial analyzer VITEK Compact-2 and confirmed by its specific gene pbp2B using regular polymerase chain reaction (PCR) method. Then PCR method was used to detect the carriers of the virulence genes ply, lytA and nanA, and calculated the fatality and hospitalization days of patients.Results Positive rates of virulence genes ply, lytA and nanA were 95.9%, 96.6% and 88.4% respectively for 147 strains isolated from sputum, and were 100.0%, 100.0% and 79.2% respectively for those from blood. Between the 147 children with pneumonia and 24 children with septicemia, there was no statistically significant difference in fatality [ 8.3% (2/24) vs. 0.7% (1/147), P=0.052], but there was a significant difference in length of hospital stay [(14.2±2.4) vs. (6.4±1.5) d; t=21.303, P<0.001].Conclusions The positive rate of SP virulence gene nanA is lower than those of ply and lytA. The positive rates of SP virulence genes ply, lytA and nanA are similar from different sources. Significant difference may be found for hospitalization days among different types of SP infections.

Citation： GAO Bing, ZHANG Hongbo, ZHANG Chi, PENG Zheng. Study on the positive rates of several virulence genes in 171 clinical strains of Streptococcus pneumoniae. West China Medical Journal, 2019, 34(9): 1042-1046. doi: 10.7507/1002-0179.201906078 Copy

1.	Centers for Disease Control Prevention. Pneumococcal disease. (2017-09-06)[2019-05-01]. https://www.cdc.gov/pneumococcal/index.html.
2.	Brooks Lavida RK, Mias GI. Streptococcus pneumoniae’s virulence and host immunity: aging, diagnostics, and prevention. Front Immunol, 2018, 9: 1366.
3.	World Health Organization Media Centre. Pneumonia: fact sheet No 331. 2013.
4.	World Health Organization. Antimicrobial resistance: global report on surveillance. (2014-04)[2019-06-03]. https://www.who.int/drugresistance/documents/surveillancereport/en/.
5.	Feldman C, Anderson R. Review: current and new generation pneumococcal vaccines. J Infect, 2014, 69(4): 309-325.
6.	Weiser JN, Ferreira DM, Paton JC. Streptococcus pneumoniae: transmission, colonization and invasion. Nat Rev Microbiol, 2018, 16(6): 355-367.
7.	魏桂芳, 沈荣. 肺炎链球菌相关毒力因子及其作用的研究进展. 微生物学免疫学进展, 2016, 44(5): 69-75.
8.	Hu DK, Liu Y, Li XY, et al. In vitro expression of Streptococcus pneumoniae ply gene in human monocytes and pneumocytes. Eur J Med Res, 2015, 20(1): 52.
9.	du Plessis M, Smith AM, Klugman KP. Rapid detection of penicillin-resistant Streptococcus pneumoniae in cerebrospinal fluid by a seminested-PCR strategy. J Clin Microbiol, 1998, 36(2): 453-457.
10.	胡大康, 张瑾, 刘池波, 等. 肺炎链球菌上调THP- 1 细胞分泌细胞间黏附分子1. 中国微生态学杂志, 2013, 25(6): 643-646, 651.
11.	Suzuki N, Yuyama M, Maeda S, et al. Genotypic identification of presumptive Streptococcus pneumoniae by PCR using four genes highly specific for S.pneumoniae. J Med Microbiol, 2006, 55(6): 709-714.
12.	Pettigrew MM, Fennie KP, York MP, et al. Variation in the presence of neuraminidase genes among Streptococcus pneumoniae isolates with identical sequence types. Infect Immun, 2006, 74(6): 3360-3365.
13.	董一山, 黄文祥, 罗涛, 等. 临床分离肺炎链球菌的毒力基因及血清型分析. 中华微生物和免疫学杂志, 2009, 29(2): 177-180.
14.	Zhao Wantong, Pan Fen, Wang Bingjie, et al. Epidemiology characteristics of streptococcus pneumoniae from children with pneumonia in shanghai: a retrospective study. Front Cell Infect Microbiol, 2019, 9(7): 258.
15.	Palmer M. The family of thiol-activated, cholesterol-binding cytolysins. Toxicon, 2001, 39(11): 1681-1689.
16.	Hirst RA, Kadioglu A, O’Callaghan C, et al. The role of pneumolysin in pneumococcal pneumonia and meningitis. Clin Exp Immunol, 2004, 138(2): 195-201.
17.	Marriott HM, Mitchell TJ, Dockrell DH. Pneumolysin: a double-edged sword during the host-pathogen interaction. Curr Mol Med, 2008, 8(6): 497-509.
18.	Mitchell TJ, Andrew PW, Saunders FK, et al. Complement activation and antibody binding by pneumolysin via a region of the toxin homologous to a human acute-phase protein. Mol Microbiol, 1991, 5(8): 1883-1888.
19.	Rossjohn J, Gilbert RJ, Crane D, et al. The molecular mechanism of pneumolysin, a virulence factor from Streptococcus pneumonia. J Mol Biol, 1998, 284(2): 449-461.
20.	Paton JC, Ferrante A. Inhibition of human polymorphonuclear leukocyte respiratory burst, bactericidal activity, and migration by pneumolysin. Infect Immun, 1983, 41(3): 1212-1216.
21.	Rogers PD, Thornton J, Barker KS, et al. Pneumolysin-dependent and -independent gene expression identified by cDNA microarray analysis of THP-1 human mononuclear cells stimulated by Streptococcus pneumonia. Infect Immun, 2003, 71(4): 2087-2094.
22.	Uchiyama S, Carlin AF, Khosravi A, et al. The surface-anchored NanA protein promotes pneumococcal brain endothelial cell invasion. J Exp Med, 2009, 206(9): 1845-1852.
23.	Nagai K, Shibasaki Y, Hasegawa K, et al. Evaluation of PCR primers to screen for Streptococcus pneumoniae isolates and β-lactam resistance, and to detect common macrolide resistance determinants. J Antimicrob Chemother, 2001, 48(6): 915-918.
24.	王愿, 蔡培泉, 姜秀峰, 等. 肺炎链球菌毒力基因检测与临床研究. 中华医院感染学杂志, 2016, 26(8): 1684-1686, 1735.
25.	Hu DK, Wang DG, Liu Y, et al. Roles of virulence genes (PsaA and CpsA) on the invasion of Streptococcus pneumoniae into blood system. Eur J Med Res, 2013, 18: 14.
26.	Weisfelt M, van de Beek D, Spanjaard L, et al. Clinical features, complications, and outcome in adults with pneumococcal meningitis: a prospective case series. Lancet Neurol, 2006, 5(2): 123-129.
27.	Kim SH, Song JH, Chung DR, et al. Changing trends in antimicrobial resistance and serotypes of Streptococcus pneumoniae isolates in Asian countries: an Asian Network for Surveillance of Resistant Pathogens (ANSORP) study. Antimicrob Agents Chemother, 2012, 56(3): 1418-1426.

1. Centers for Disease Control Prevention. Pneumococcal disease. (2017-09-06)[2019-05-01]. https://www.cdc.gov/pneumococcal/index.html.
2. Brooks Lavida RK, Mias GI. Streptococcus pneumoniae’s virulence and host immunity: aging, diagnostics, and prevention. Front Immunol, 2018, 9: 1366.
3. World Health Organization Media Centre. Pneumonia: fact sheet No 331. 2013.
4. World Health Organization. Antimicrobial resistance: global report on surveillance. (2014-04)[2019-06-03]. https://www.who.int/drugresistance/documents/surveillancereport/en/.
5. Feldman C, Anderson R. Review: current and new generation pneumococcal vaccines. J Infect, 2014, 69(4): 309-325.
6. Weiser JN, Ferreira DM, Paton JC. Streptococcus pneumoniae: transmission, colonization and invasion. Nat Rev Microbiol, 2018, 16(6): 355-367.
7. 魏桂芳, 沈荣. 肺炎链球菌相关毒力因子及其作用的研究进展. 微生物学免疫学进展, 2016, 44(5): 69-75.
8. Hu DK, Liu Y, Li XY, et al. In vitro expression of Streptococcus pneumoniae ply gene in human monocytes and pneumocytes. Eur J Med Res, 2015, 20(1): 52.
9. du Plessis M, Smith AM, Klugman KP. Rapid detection of penicillin-resistant Streptococcus pneumoniae in cerebrospinal fluid by a seminested-PCR strategy. J Clin Microbiol, 1998, 36(2): 453-457.
10. 胡大康, 张瑾, 刘池波, 等. 肺炎链球菌上调THP- 1 细胞分泌细胞间黏附分子1. 中国微生态学杂志, 2013, 25(6): 643-646, 651.
11. Suzuki N, Yuyama M, Maeda S, et al. Genotypic identification of presumptive Streptococcus pneumoniae by PCR using four genes highly specific for S.pneumoniae. J Med Microbiol, 2006, 55(6): 709-714.
12. Pettigrew MM, Fennie KP, York MP, et al. Variation in the presence of neuraminidase genes among Streptococcus pneumoniae isolates with identical sequence types. Infect Immun, 2006, 74(6): 3360-3365.
13. 董一山, 黄文祥, 罗涛, 等. 临床分离肺炎链球菌的毒力基因及血清型分析. 中华微生物和免疫学杂志, 2009, 29(2): 177-180.
14. Zhao Wantong, Pan Fen, Wang Bingjie, et al. Epidemiology characteristics of streptococcus pneumoniae from children with pneumonia in shanghai: a retrospective study. Front Cell Infect Microbiol, 2019, 9(7): 258.
15. Palmer M. The family of thiol-activated, cholesterol-binding cytolysins. Toxicon, 2001, 39(11): 1681-1689.
16. Hirst RA, Kadioglu A, O’Callaghan C, et al. The role of pneumolysin in pneumococcal pneumonia and meningitis. Clin Exp Immunol, 2004, 138(2): 195-201.
17. Marriott HM, Mitchell TJ, Dockrell DH. Pneumolysin: a double-edged sword during the host-pathogen interaction. Curr Mol Med, 2008, 8(6): 497-509.
18. Mitchell TJ, Andrew PW, Saunders FK, et al. Complement activation and antibody binding by pneumolysin via a region of the toxin homologous to a human acute-phase protein. Mol Microbiol, 1991, 5(8): 1883-1888.
19. Rossjohn J, Gilbert RJ, Crane D, et al. The molecular mechanism of pneumolysin, a virulence factor from Streptococcus pneumonia. J Mol Biol, 1998, 284(2): 449-461.
20. Paton JC, Ferrante A. Inhibition of human polymorphonuclear leukocyte respiratory burst, bactericidal activity, and migration by pneumolysin. Infect Immun, 1983, 41(3): 1212-1216.
21. Rogers PD, Thornton J, Barker KS, et al. Pneumolysin-dependent and -independent gene expression identified by cDNA microarray analysis of THP-1 human mononuclear cells stimulated by Streptococcus pneumonia. Infect Immun, 2003, 71(4): 2087-2094.
22. Uchiyama S, Carlin AF, Khosravi A, et al. The surface-anchored NanA protein promotes pneumococcal brain endothelial cell invasion. J Exp Med, 2009, 206(9): 1845-1852.
23. Nagai K, Shibasaki Y, Hasegawa K, et al. Evaluation of PCR primers to screen for Streptococcus pneumoniae isolates and β-lactam resistance, and to detect common macrolide resistance determinants. J Antimicrob Chemother, 2001, 48(6): 915-918.
24. 王愿, 蔡培泉, 姜秀峰, 等. 肺炎链球菌毒力基因检测与临床研究. 中华医院感染学杂志, 2016, 26(8): 1684-1686, 1735.
25. Hu DK, Wang DG, Liu Y, et al. Roles of virulence genes (PsaA and CpsA) on the invasion of Streptococcus pneumoniae into blood system. Eur J Med Res, 2013, 18: 14.
26. Weisfelt M, van de Beek D, Spanjaard L, et al. Clinical features, complications, and outcome in adults with pneumococcal meningitis: a prospective case series. Lancet Neurol, 2006, 5(2): 123-129.
27. Kim SH, Song JH, Chung DR, et al. Changing trends in antimicrobial resistance and serotypes of Streptococcus pneumoniae isolates in Asian countries: an Asian Network for Surveillance of Resistant Pathogens (ANSORP) study. Antimicrob Agents Chemother, 2012, 56(3): 1418-1426.

Previous Article
Bioinformatics and functional analysis of key genes and pathways in tuberculosis
Next Article
Research progress of type Ⅰ collagen in osteoporosis

West China Medical Journal

Study on the positive rates of several virulence genes in 171 clinical strains of Streptococcus pneumoniae

Abstract Full text Figures/Tables Video References Cited by

Previous Article

Next Article

Format

Content