Distribution of human papilloma virus subtypes detected by flow fluorescence hybridization in physical examination women in West China Hospital of Sichuan University_West China Medical Journal

Authors：

ZHONG Huiyu , LIU Tangyuheng , WANG Minjin , WU Lijuan , ZHUANG Jie , WANG Jun ,  SONG Xingbo

Department of Laboratory Medicine, West China Hospital, Sichuan University, Chengdu, Sichuan 610041, P. R. China;

Corresponding author：

SONG Xingbo, Email: songxingbo1981@163.com

Keywords：

Human papilloma virus; Flow fluorescent hybridization technology; Physical examination population

DOI：

10.7507/1002-0179.201907058

Video：

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Objective Using molecular biology method to detect and genotype human papilloma virus (HPV) in women taking physical examination in West China Hospital, Sichuan University, to explore the infection status and genotype distribution of HPV in normal women in Chengdu area, and to provide basis for early effective prevention and control of cervical cancer and domestic research and development of HPV vaccine. Methods Flow fluorescent hybridization technique was used to detect and genotype HPV-DNA in 25 148 healthy women taking physical examination in West China Hospital, Sichuan University between May 1st, 2018 and May 31st, 2019. The overall positive HPV infection rate, HPV genotype distribution, and characteristics of HPV infections were analyzed and calculated, and the HPV infection rates of different age groups were calculated and compared by chi-square test using SPSS 17.0 software. Results The overall positive rate of HPV infection was 12.19% (3 066/25 148). The high-risk HPV genotypes infection rate was 8.69% (2 186/25 148), and the top five subtypes with the highest infection rates were HPV52, HPV53, HPV58, HPV16, and HPV39. The low-risk HPV genotypes infection rate was 4.66% (1 171/25 148), and the top five subtypes with the highest infection rates were HPV61, HPV81, HPV43, HPV44, and HPV6. Single subtype infections were the main infections with a proportion of 81.74% (2 506/3 066), and the most common multiple infections were double infections which accounted for 13.96% (428/3 066). In different age groups, the HPV infection rate of group 60-69 was the highest (12.87%), while that of group 70-89 was the lowest (10.88%), but the difference among different age groups was not statistically significant (χ²=4.035, P=0.544). Conclusion According to the results of this study in women taking physical examination in West China Hospital, Sichuan University, we suggest adding HPV52, HPV53, and HPV58 which have the highest infection rate in high-risk HPV subtypes to the evaluation of domestic HPV vaccine screening and the cervical cancer prevention and control system.

Citation： ZHONG Huiyu, LIU Tangyuheng, WANG Minjin, WU Lijuan, ZHUANG Jie, WANG Jun, SONG Xingbo. Distribution of human papilloma virus subtypes detected by flow fluorescence hybridization in physical examination women in West China Hospital of Sichuan University. West China Medical Journal, 2019, 34(8): 895-899. doi: 10.7507/1002-0179.201907058 Copy

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14.	张美娟, 成祥君, 邱胜丰. 南京地区女性高危型 HPV 亚型分布特征及与宫颈病变的临床分析. 国际检验医学杂志, 2019, 40(10): 1258-1261.
15.	张亚丽, 彭仁佳, 范毅凯, 等. 郑州地区 22708 例女性 HPV 感染及基因亚型分布研究. 检验医学与临床, 2019, 16(11): 1537-1540, 1543.
16.	刘玉萍, 沈太敏, 帅平, 等. 成都市健康体检妇女人乳头状瘤病毒感染及基因亚型分析. 实用医院临床杂志, 2015, 12(6): 52-54.
17.	赵玉萍, 王中才, 闫艺, 等. 新疆昌吉地区 1100 例妇女 HPV 感染情况及其亚型分型. 新疆医学, 2017, 47(12): 1388-1390.
18.	李芳芹, 汪敏, 杜伟平. 719 例女性 HPV 感染情况及其亚型分析. 延安大学学报(医学科学版), 2017, 15(4): 55-57, 60.
19.	蔡鹏飞, 许泽辉, 王敬仁, 等. 广西柳州地区 9810 例人乳头瘤病毒检测结果分析. 检验医学, 2019, 34(3): 246-248.
20.	郑红云, 杨鹏, 申复进, 等. 7746 例妇女宫颈脱落细胞 HPV 基因分型结果研究. 国际检验医学杂志, 2017, 38(24): 3391-3392, 3395.

1. Schiffman M, Clifford G, Buonaguro FM. Classification of weakly carcinogenic human papillomavirus types: addressing the limits of epidemiology at the borderline. Infect Agent Cancer, 2009, 4(1): 8.
2. Torre LA, Bray F, Siegel RL, et al. Global cancer statistics, 2012. CA Cancer J Clin, 2015, 65(2): 87-108.
3. 耿建祥, 黄华艺, 刘建华, 等. HPV 感染疾病相关问题专家共识 (2017). 医学研究生学报, 2017, 30(12): 1238-1241.
4. Couto E, Saeterdal I, Juvet LK, et al. HPV catch-up vaccination of young women: a systematic review and meta-analysis. BMC Public Health, 2014, 14: 867.
5. WHO/ICO Information Centre on HPV and Cervical Cancer. HPV and cervical cancer in the 2007 report. Vaccine, 2007, 25(Suppl 3): C1-C230.
6. Zeng Z, Yang H, Li Z, et al. Prevalence and genotype distribution of HPV infection in China: analysis of 51,345 HPV genotyping results from China’s largest CAP certified laboratory. J Cancer, 2016, 7(9): 1037-1043.
7. 吴海燕, 肖琳, 周琴, 等. 成都北部地区女性人乳头瘤病毒感染特征分析. 成都医学院学报, 2019(3): 393-396, 400.
8. 方莉, 叶红, 许媛, 等. 四川东北部地区女性高危型人乳头瘤病毒感染情况分析. 检验医学与临床, 2017, 14(3): 352-354.
9. 赵佳, 何玉霞, 杜利君. 四川南充地区 3762 例女性高危型人乳头瘤病毒感染结果分析. 中国计划生育和妇产科, 2018, 10(8): 94-96.
10. 张建海, 王前, 王巍, 等. 北京市大兴区 2117 例女性宫颈人乳头瘤病毒感染基因亚型状况分析. 实用预防医学, 2019, 26(4): 468-470.
11. 王燕, 吴珍珍, 周庆云. 甘肃省妇女宫颈 HPV 感染的流行病学特征. 中国妇幼保健, 2010, 25(36): 5371-5373.
12. 郎妙双, 马陈美, 孙丽萍. 宫颈人乳头瘤病毒感染调查与危险因素分析. 中国预防医学杂志, 2019, 20(5): 442-445.
13. 吴颖, 曹芬芳, 刘太林, 等. 湖南地区 7076 例女性 HPV 感染情况及分型分析. 实用预防医学, 2019, 26(4): 474-477.
14. 张美娟, 成祥君, 邱胜丰. 南京地区女性高危型 HPV 亚型分布特征及与宫颈病变的临床分析. 国际检验医学杂志, 2019, 40(10): 1258-1261.
15. 张亚丽, 彭仁佳, 范毅凯, 等. 郑州地区 22708 例女性 HPV 感染及基因亚型分布研究. 检验医学与临床, 2019, 16(11): 1537-1540, 1543.
16. 刘玉萍, 沈太敏, 帅平, 等. 成都市健康体检妇女人乳头状瘤病毒感染及基因亚型分析. 实用医院临床杂志, 2015, 12(6): 52-54.
17. 赵玉萍, 王中才, 闫艺, 等. 新疆昌吉地区 1100 例妇女 HPV 感染情况及其亚型分型. 新疆医学, 2017, 47(12): 1388-1390.
18. 李芳芹, 汪敏, 杜伟平. 719 例女性 HPV 感染情况及其亚型分析. 延安大学学报(医学科学版), 2017, 15(4): 55-57, 60.
19. 蔡鹏飞, 许泽辉, 王敬仁, 等. 广西柳州地区 9810 例人乳头瘤病毒检测结果分析. 检验医学, 2019, 34(3): 246-248.
20. 郑红云, 杨鹏, 申复进, 等. 7746 例妇女宫颈脱落细胞 HPV 基因分型结果研究. 国际检验医学杂志, 2017, 38(24): 3391-3392, 3395.

West China Medical Journal

Distribution of human papilloma virus subtypes detected by flow fluorescence hybridization in physical examination women in West China Hospital of Sichuan University

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