Effect and mechanism of curcumin on lipopolysaccharide-induced pulmonary macrophage inflammation and apoptosis_West China Medical Journal

Authors：

WANG Youli ,  CHEN Juping , WAN Yunqiang

Department of Respiratory Medicine, Affiliated Hospital of Southwest Medical University, Luzhou, Sichuan 646000, P. R. China;

Corresponding author：

CHEN Juping, Email: 849946999@qq.com

Keywords：

MicroRNA-132; High mobility group protein B1; Curcumin; Lipopolysaccharide; Alveolar macrophages; Inflammation; Apoptosis

DOI：

10.7507/1002-0179.201910090

Video：

Export PDF Favorites Scan Get Citation

Abstract Full text Figures/Tables Video References Cited by

Objective To investigate the effect of curcumin on lipopolysaccharide (LPS)-induced inflammation and apoptosis in alveolar macrophage via microRNA-132 (miR-132)/high mobility group protein B1 (HMGB1).Methods The cultured mouse alveolar macrophage line (RAW264.7 cells) were divided into the control group, the LPS group, the LPS+50 μmol/L curcumin group, and the LPS+100 μmol/L curcumin group. Forty-eight hours after drug treatment, the levels of miR-132/HMGB1, inflammatory mediator and apoptotic were detected. Secondly, the empty vector, synthetic miR-132 mimics and inhibitors were transfected into another cultured mouse alveolar macrophage line (RAW264.7 cells) to detect the inflammation and apoptosis of alveolar macrophage after transfection.Results Compared with the control group, in the LPS group, the apoptosis of alveolar macrophage, the levels of interleukin (IL)-6, IL-8 and tumor necrosis factor (TNF)-α, and the expression of miR-132 increased, while the expression of HMGB1 decreased (P<0.05); compared with the LPS group, in the two curcumin groups, the apoptosis of alveolar macrophage, the levels of IL-6, IL-8 and TNF-α, and the expression of miR-132 decreased, while the expression of HMGB1 increased (P<0.05); and the greater the drug concentration, the more obvious the effect (P<0.05). In addition, up-regulation of miR-132 reduced the expression of HMGB1 in alveolar macrophage, increased inflammatory factor, and induced apoptosis in alveolar macrophage; however, down-regulation of miR-132 increased the expression of HMGB1 in alveolar macrophage, reduced inflammatory factor, and inhibited apoptosis in alveolar macrophage (P<0.05).Conclusion Curcumin could decrease LPS-induced inflammation and apoptosis in alveolar macrophage via decreasing miR-132 and increasing HMGB1.

Citation： WANG Youli, CHEN Juping, WAN Yunqiang. Effect and mechanism of curcumin on lipopolysaccharide-induced pulmonary macrophage inflammation and apoptosis. West China Medical Journal, 2020, 35(2): 192-197. doi: 10.7507/1002-0179.201910090 Copy

1.	Lapara NJ 3rd, Kelly BL. Suppression of LPS-induced inflammatory responses in macrophages infected with leishmania. J Inflamm (Lond), 2010, 7(1): 8.
2.	Zhao S, Ma L, Cao C, et al. Curcumin-loaded redox response of self-assembled micelles for enhanced antitumor and anti-inflammation efficacy. Int J Nanomedicine, 2017, 12: 2489-2504.
3.	Sarkar A, De R, Mukhopadhyay AK. Curcumin as a potential therapeutic candidate for helicobacter pylori associated diseases. World J Gastroenterol, 2016, 22(9): 2736-2748.
4.	Cohen JE, Lee PR, Chen S, et al. MicroRNA regulation of homeostatic synaptic plasticity. Proc Natl Acad Sci USA, 2011, 108(28): 11650-11655.
5.	Liu F, Li Y, Jiang R, et al. miR-132 inhibits lipopolysaccharide-induced inflammation in alveolar macrophages by the cholinergic anti-inflammatory pathway. Exp Lung Res, 2015, 41(5): 261-269.
6.	Chen J, Li G. MiR-1284 enhances sensitivity of cervical cancer cells to cisplatin via downregulating HMGB1. Biomed Pharmacother, 2018, 107: 997-1003.
7.	Wu D, Pan P, Su X, et al. Interferon regulatory factor-1 mediates alveolar macrophage pyroptosis during LPS-induced acute lung injury in mice. Shock, 2016, 46(3): 329-338.
8.	Chen S, Yuan J, Yao S, et al. Lipopolysaccharides may aggravate apoptosis through accumulation of autophagosomes in alveolar macrophages of human silicosis. Autophagy, 2015, 11(12): 2346-2357.
9.	Yu JH, Long L, Luo ZX, et al. Anti-inflammatory role of microRNA let-7c in LPS treated alveolar macrophages by targeting STAT3. Asian Pac J Trop Med, 2016, 9(1): 72-75.
10.	Zhang M, Xie Y, Yan R, et al. Curcumin ameliorates alveolar epithelial injury in a rat model of chronic obstructive pulmonary disease. Life Sci, 2016, 164: 1-8.
11.	Liu X, Meng J. Luteolin alleviates LPS-induced bronchopneumonia injury in vitro and in vivo by down-regulating microRNA-132 expression. Biomed Pharmacother, 2018, 106: 1641-1649.
12.	Ye M, Zhang J, Zhang J, et al. Curcumin promotes apoptosis by activating the p53-miR-192-5p/215-XIAP pathway in non-small cell lung cancer. Cancer Lett, 2015, 357(1): 196-205.
13.	Wang J, Wang H, Shi J, et al. Effects of bone marrow MSCs transfected with sRAGE on the intervention of HMGB1 induced immuno-inflammatory reaction. Int J Clin Exp Pathol, 2015, 8(10): 12028-12040.
14.	Wang F, Lu Z, Hawkes M, et al. Fas (CD95) induces rapid, TLR4/IRAK4-dependent release of pro-inflammatory HMGB1 from macrophages. J Inflamm (Lond), 2010, 7: 30.
15.	Hung YL, Fang SH, Wang SC, et al. Corylin protects LPS-induced sepsis and attenuates LPS-induced inflammatory response. Sci Rep, 2017, 7: 46299.

1. Lapara NJ 3rd, Kelly BL. Suppression of LPS-induced inflammatory responses in macrophages infected with leishmania. J Inflamm (Lond), 2010, 7(1): 8.
2. Zhao S, Ma L, Cao C, et al. Curcumin-loaded redox response of self-assembled micelles for enhanced antitumor and anti-inflammation efficacy. Int J Nanomedicine, 2017, 12: 2489-2504.
3. Sarkar A, De R, Mukhopadhyay AK. Curcumin as a potential therapeutic candidate for helicobacter pylori associated diseases. World J Gastroenterol, 2016, 22(9): 2736-2748.
4. Cohen JE, Lee PR, Chen S, et al. MicroRNA regulation of homeostatic synaptic plasticity. Proc Natl Acad Sci USA, 2011, 108(28): 11650-11655.
5. Liu F, Li Y, Jiang R, et al. miR-132 inhibits lipopolysaccharide-induced inflammation in alveolar macrophages by the cholinergic anti-inflammatory pathway. Exp Lung Res, 2015, 41(5): 261-269.
6. Chen J, Li G. MiR-1284 enhances sensitivity of cervical cancer cells to cisplatin via downregulating HMGB1. Biomed Pharmacother, 2018, 107: 997-1003.
7. Wu D, Pan P, Su X, et al. Interferon regulatory factor-1 mediates alveolar macrophage pyroptosis during LPS-induced acute lung injury in mice. Shock, 2016, 46(3): 329-338.
8. Chen S, Yuan J, Yao S, et al. Lipopolysaccharides may aggravate apoptosis through accumulation of autophagosomes in alveolar macrophages of human silicosis. Autophagy, 2015, 11(12): 2346-2357.
9. Yu JH, Long L, Luo ZX, et al. Anti-inflammatory role of microRNA let-7c in LPS treated alveolar macrophages by targeting STAT3. Asian Pac J Trop Med, 2016, 9(1): 72-75.
10. Zhang M, Xie Y, Yan R, et al. Curcumin ameliorates alveolar epithelial injury in a rat model of chronic obstructive pulmonary disease. Life Sci, 2016, 164: 1-8.
11. Liu X, Meng J. Luteolin alleviates LPS-induced bronchopneumonia injury in vitro and in vivo by down-regulating microRNA-132 expression. Biomed Pharmacother, 2018, 106: 1641-1649.
12. Ye M, Zhang J, Zhang J, et al. Curcumin promotes apoptosis by activating the p53-miR-192-5p/215-XIAP pathway in non-small cell lung cancer. Cancer Lett, 2015, 357(1): 196-205.
13. Wang J, Wang H, Shi J, et al. Effects of bone marrow MSCs transfected with sRAGE on the intervention of HMGB1 induced immuno-inflammatory reaction. Int J Clin Exp Pathol, 2015, 8(10): 12028-12040.
14. Wang F, Lu Z, Hawkes M, et al. Fas (CD95) induces rapid, TLR4/IRAK4-dependent release of pro-inflammatory HMGB1 from macrophages. J Inflamm (Lond), 2010, 7: 30.
15. Hung YL, Fang SH, Wang SC, et al. Corylin protects LPS-induced sepsis and attenuates LPS-induced inflammatory response. Sci Rep, 2017, 7: 46299.

Previous Article
Clinical experience analysis of robotic hysterectomy in 62 patients with large uteri
Next Article
The role of hierarchical diagnosis and treatment in the perfection of day surgery management

West China Medical Journal

Effect and mechanism of curcumin on lipopolysaccharide-induced pulmonary macrophage inflammation and apoptosis

Abstract Full text Figures/Tables Video References Cited by

Previous Article

Next Article

Format

Content