Application of BenchMark automatic special staining instrument in reticular fiber staining of liver biopsy_West China Medical Journal

Authors：

CHEN Yu ¹ , YANG Mei ¹ , WANG Rong ¹ , LIAO Hongyan ² , MAO Zhigang ² ,  ZHENG Qin ²

1. Department of Pathology, West China Hospital, Sichuan University, Chengdu, Sichuan 610041, P. R. China;
2. Department of Laboratory Medicine, West China Hospital, Sichuan University, Chengdu, Sichuan 610041, P. R. China;

Corresponding author：

ZHENG Qin, Email: zhengqinhx@scu.edu.cn

Keywords：

BenchMark automatic special staining instrument; Reticular fiber staining; Foot staining; Standard operating procedure

DOI：

10.7507/1002-0179.201912082

Video：

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Objective To explore the dyeing conditions of reticular fiber staining of liver puncture tissue stained by BenchMark automatic special staining instrument, evaluate the staining effect, and share the using experience.Methods A total of 30 cases of liver puncture specimens from April to May 2019 in West China Hospital of Sichuan University were selected. They were fixed with 4% neutral formaldehyde fix solution, treated with automatic dehydrator, embedded in paraffin, stained with adhesive slides, and baked in 65℃ oven for 30 min. Each specimen was sliced in duplicate, with a thickness of 5 μm. One was reticular fiber stained with BenchMark automatic special staining instrument under the dyeing condition according to the using experience of the laboratory, and the other was reticular fiber stained by manual Foot staining method according to the standard operating procedure of the laboratory. The staining effect under microscope was observed by a chief pathologist with extensive slide-reading experience.Results The success rate of instrumental method was 100.0% (30/30), and that of the manual method was 76.7% (23/30). The difference in success rate between the two methods was statistically significant (χ²=5.143, P=0.023).Conclusion The reticular fiber staining effect of BenchMark automatic special staining instrument is stable and reliable, and the success rate is higher than that of manual Foot staining.

Citation： CHEN Yu, YANG Mei, WANG Rong, LIAO Hongyan, MAO Zhigang, ZHENG Qin. Application of BenchMark automatic special staining instrument in reticular fiber staining of liver biopsy. West China Medical Journal, 2020, 35(9): 1090-1093. doi: 10.7507/1002-0179.201912082 Copy

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2. 姜晶, 刘楠楠, 张继, 等. 特殊染色在肝穿刺活检组织中的应用价值. 北华大学学报(自然科学版), 2015, 16(4): 476-480.
3. 王晓燕, 高俊岩, 王艳霞. 网状纤维染色在临床病理诊断中的应用及技术. 中国医药导报, 2016, 13(9): 72-75.
4. 郑海燕, 方庆全, 索文昊. 网状纤维染色技术改进. 中国组织化学与细胞化学杂志, 2018, 27(1): 61-65.
5. 解立武, 常芳, 卢小波. 经典和改良Gomori网状纤维染色的比较. 中国药物与临床, 2018, 18(1): 31-32.
6. 张红雷, 邵晨, 王泰龄. 网状纤维染色-渡边改良法的应用体会. 诊断病理学杂志, 2018, 25(8): 593-594.
7. 冯倩倩, 李纯, 关正元, 等. 网状纤维染色在BenchMark特殊染色系统中的优势. 诊断病理学杂志, 2018, 25(1): 74-75.
8. 于芳, 李纯. Dakewe DP260全自动智能染色机在细胞染色中的使用体会. 诊断病理学杂志, 2017, 24(3): 205, 209.
9. 王伯沄, 李玉松, 黄高晟, 等. 病理学技术. 北京: 人民卫生出版社, 2000: 145.
10. 王德田, 董建强. 实用现代病理学技术. 北京: 中国协和医科大学出版社, 2012: 94-95.
11. 赖美娜, 苏学劲. 浅谈网状纤维染色失误原因及对策. 实用医技杂志, 2007, 14(20): 2793.
12. 曹阳, 罗虹, 罗人滋. 网状纤维染色中脱片的解决方案. 广西医科大学学报, 2003, 20(6): 975.
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14. 王玉芳, 徐晓艳, 武彦. 常用特殊染色在病理诊断中的应用. 实用医技杂志, 2013, 20(12): 1357-1358.
15. 中华医学会. 临床技术操作规范病理学分册. 北京: 人民军医出版社, 2004: 37-38.
16. 王炳海, 方庆全, 叶美华. 提高网状纤维染色病理实验教学质量的探讨. 齐齐哈尔医学院学报, 2013, 34(7): 1029-1030.

West China Medical Journal

Application of BenchMark automatic special staining instrument in reticular fiber staining of liver biopsy

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