Effect of corn oligopeptide on dexamethasone-induced muscle atrophy_West China Medical Journal

Authors：

WANG Yunfeng ¹ , YAN Jiuming ² , ZHOU Zhengming ¹ , GUO Jiankui ¹ , WEI Yaoyao ¹ , JING Xiaofan ² , LIAO Xinyi ² ,  HU Wen ^1,2

1. West China School of Public Health / West China Fourth Hospital, Sichuan University, Chengdu, Sichuan 610041, P. R. China;
2. Department of Clinical Nutrition, West China Hospital, Sichuan University, Chengdu, Sichuan 610041, P. R. China;

Corresponding author：

HU Wen, Email: wendy_nutrition@163.com

Keywords：

Corn oligopeptide; sarcopenia; dexamethasone; metabolomics

DOI：

10.7507/1002-0179.202205119

Video：

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Objective To explore the effect of corn oligopeptide (COP) on dexamethasone-induced muscle atrophy. Methods Forty-nine male Sprague-Dawley rats aged 8 weeks were divided into blank group (n=10) and model group (n=39). The rats in the model group were intraperitoneally injected with dexamethasone (1.0 mg/kg), and the rats in the blank group were injected with normal saline. After 19 days, one rat in the blank group and three rats in the model group were taken to observe whether the model was successfully constructed. After successful modeling, the rats in the model group were randomly divided into model control group, COP low-dose group (COP-L group, 0.5 g/kg), COP medium-dose group (COP-M group, 1.0 g/kg) and COP high-dose group (COP-H group, 2.0 g/kg), with 9 rats in each group. After 33 days, the grip strength of the rats was measured, and then the gastrocnemius, soleus, tibialis anterior and metatarsal muscles were separated and weighed, and muscle fiber diameter, relative expression of Atrogin-1 and MuRF-1 mRNA were measured. Non-targeted metabolomics of gastrocnemius muscle were measured. Results Compared with that in the blank group, the body weight of rats in the model group reduced (P<0.05), and myofibril rupture was observed, indicating that the model was successful. Compared with those in the model control group, the grip strength increased in the COP-L and COP-M groups (P<0.05); the muscle coefficients of gastrocnemius and soleus in the COP-L and COP-H groups increased (P<0.05), and the muscle coefficients of plantaris in the COP-L and COP-M groups increased (P<0.05); the muscle fiber diameter of the tibial anterior muscle increased in the three doses of COP groups (P<0.05), and the muscle fiber diameter of the plantaris muscle increased in the COP-M and COP-H groups (P<0.05); the relative expression of Atrogin-1 mRNA decreased in the three doses of COP groups (P<0.05), while the relative expression of MurF-1 mRNA in the COP-L and COP-H groups decreased (P<0.05). The amino acid synthesis pathway, glycolysis pathway, and acid metabolism pathway were activated in gastrocnemius muscle. Conclusions COP can significantly improve the muscle atrophy induced by dexamethasone. The mechanism may be related to the decrease of Atrogin-1 and MuRF-1 expression in ubiquitin-proteasome pathway and the increase of amino acid biosynthesis.

Citation： WANG Yunfeng, YAN Jiuming, ZHOU Zhengming, GUO Jiankui, WEI Yaoyao, JING Xiaofan, LIAO Xinyi, HU Wen. Effect of corn oligopeptide on dexamethasone-induced muscle atrophy. West China Medical Journal, 2023, 38(3): 429-437. doi: 10.7507/1002-0179.202205119 Copy

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4.	孙建琴, 张坚, 常翠青, 等. 肌肉衰减综合征营养与运动干预中国专家共识(节录). 营养学报, 2015, 37(4): 320-324.
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9.	刘文颖, 林峰, 金振涛, 等. 玉米低聚肽的体外抗氧化作用. 食品科学, 2011, 32(5): 22-26.
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15.	Fuchs CJ, Hermans WJH, Holwerda AM, et al. Branched-chain amino acid and branched-chain ketoacid ingestion increases muscle protein synthesis rates in vivo in older adults: a double-blind, randomized trial. Am J Clin Nutr, 2019, 110(4): 862-872.
16.	Mobley CB, Fox CD, Ferguson BS, et al. L-leucine, beta-hydroxy-beta-methylbutyric acid (HMB) and creatine monohydrate prevent myostatin-induced Akirin-1/Mighty mRNA down-regulation and myotube atrophy. J Int Soc Sports Nutr, 2014, 11: 38.
17.	Mobley CB, Hornberger TA, Fox CD, et al. Effects of oral phosphatidic acid feeding with or without whey protein on muscle protein synthesis and anabolic signaling in rodent skeletal muscle. J Int Soc Sports Nutr, 2015, 12: 32.
18.	Yamamoto D, Maki T, Herningtyas EH, et al. Branched-chain amino acids protect against dexamethasone-induced soleus muscle atrophy in rats. Muscle Nerve, 2010, 41(6): 819-827.
19.	Maki T, Yamamoto D, Nakanishi S, et al. Branched-chain amino acids reduce hindlimb suspension-induced muscle atrophy and protein levels of atrogin-1 and MuRF1 in rats. Nutr Res, 2012, 32(9): 676-683.
20.	Yamanashi K, Kinugawa S, Fukushima A, et al. Branched-chain amino acid supplementation ameliorates angiotensin II-induced skeletal muscle atrophy. Life Sci, 2020, 250: 117593.
21.	Kitajima Y, Yoshioka K, Suzuki N. The ubiquitin-proteasome system in regulation of the skeletal muscle homeostasis and atrophy: from basic science to disorders. J Physiol Sci, 2020, 70(1): 40.
22.	Sartori R, Romanello V, Sandri M. Mechanisms of muscle atrophy and hypertrophy: implications in health and disease. Nat Commun, 2021, 12(1): 330.
23.	Kobayashi H, Kato H, Hirabayashi Y, et al. Modulations of muscle protein metabolism by branched-chain amino acids in normal and muscle-atrophying rats. J Nutr, 2006, 136(1 Suppl): 234S-236S.
24.	Børsheim E, Bui QU, Tissier S, et al. Effect of amino acid supplementation on muscle mass, strength and physical function in elderly. Clin Nutr, 2008, 27(2): 189-195.
25.	Zhao Q, Shen H, Su KJ, et al. A joint analysis of metabolomic profiles associated with muscle mass and strength in Caucasian women. Aging (Albany NY), 2018, 10(10): 2624-2635.
26.	Murphy RA, Moore SC, Playdon M, et al. Metabolites associated with lean mass and adiposity in older black men. J Gerontol A Biol Sci Med Sci, 2017, 72(10): 1352-1359.
27.	杨蕾. 中老年人肌肉减少症与代谢综合征的相关性及代谢组学研究. 乌鲁木齐: 新疆医科大学, 2020.
28.	鲁翰霖. 基于代谢组学的早期骨骼肌损伤时间推断的研究. 太原: 山西医科大学, 2019.

1. Ding S, Dai Q, Huang H, et al. An overview of muscle atrophy. Adv Exp Med Biol, 2018, 1088: 3-19.
2. Keller K. Sarcopenia. Wien Med Wochenschr, 2019, 169(7/8): 157-172.
3. Cruz-Jentoft AJ, Sayer AA. Sarcopenia. Lancet, 2019, 393(10191): 2636-2646.
4. 孙建琴, 张坚, 常翠青, 等. 肌肉衰减综合征营养与运动干预中国专家共识(节录). 营养学报, 2015, 37(4): 320-324.
5. Olaniyan ET, O’Halloran F, McCarthy AL. Dietary protein considerations for muscle protein synthesis and muscle mass preservation in older adults. Nutr Res Rev, 2021, 34(1): 147-157.
6. 李红毅, 白树民, 黄贱英, 等. 大豆低聚肽对模拟失重大鼠肌萎缩的防护作用. 营养学报, 2012, 34(1): 46-49, 54.
7. 黄梦君, 许淑芳. 食源性低聚肽的消化吸收机制及营养功能的研究进展. 中国食物与营养, 2021, 27(10): 55-58.
8. Zdzieblik D, Oesser S, Baumstark MW, et al. Collagen peptide supplementation in combination with resistance training improves body composition and increases muscle strength in elderly sarcopenic men: a randomised controlled trial. Br J Nutr, 2015, 114(8): 1237-1245.
9. 刘文颖, 林峰, 金振涛, 等. 玉米低聚肽的体外抗氧化作用. 食品科学, 2011, 32(5): 22-26.
10. 陶景景. 游泳联合玉米低聚肽提高小鼠运动能力和促进心肌保护的作用及机制研究. 武汉: 武汉体育学院, 2019.
11. 潘兴昌, 曾瑜, 刘文颖, 等. 玉米低聚肽和姜黄素对小鼠的醒酒功能研究. 食品与发酵工业, 2016, 42(11): 25-34.
12. 林峰, 梁锐, 王军波, 等. 玉米低聚肽降血压作用的实验研究. 食品与发酵工业, 2009(8): 1-4.
13. 王梦莹, 马丽娜, 高鹤, 等. 玉米低聚肽对肥胖小鼠预防性干预的效果研究//营养研究与临床实践—第十四届全国营养科学大会暨第十一届亚太临床营养大会、第二届全球华人营养科学家大会论文摘要汇编. 南京: 中国营养学会, 2019: 477-478.
14. Wilkinson DJ, Hossain T, Limb MC, et al. Impact of the calcium form of β-hydroxy-β-methylbutyrate upon human skeletal muscle protein metabolism. Clin Nutr, 2018, 37(6 Pt A): 2068-2075.
15. Fuchs CJ, Hermans WJH, Holwerda AM, et al. Branched-chain amino acid and branched-chain ketoacid ingestion increases muscle protein synthesis rates in vivo in older adults: a double-blind, randomized trial. Am J Clin Nutr, 2019, 110(4): 862-872.
16. Mobley CB, Fox CD, Ferguson BS, et al. L-leucine, beta-hydroxy-beta-methylbutyric acid (HMB) and creatine monohydrate prevent myostatin-induced Akirin-1/Mighty mRNA down-regulation and myotube atrophy. J Int Soc Sports Nutr, 2014, 11: 38.
17. Mobley CB, Hornberger TA, Fox CD, et al. Effects of oral phosphatidic acid feeding with or without whey protein on muscle protein synthesis and anabolic signaling in rodent skeletal muscle. J Int Soc Sports Nutr, 2015, 12: 32.
18. Yamamoto D, Maki T, Herningtyas EH, et al. Branched-chain amino acids protect against dexamethasone-induced soleus muscle atrophy in rats. Muscle Nerve, 2010, 41(6): 819-827.
19. Maki T, Yamamoto D, Nakanishi S, et al. Branched-chain amino acids reduce hindlimb suspension-induced muscle atrophy and protein levels of atrogin-1 and MuRF1 in rats. Nutr Res, 2012, 32(9): 676-683.
20. Yamanashi K, Kinugawa S, Fukushima A, et al. Branched-chain amino acid supplementation ameliorates angiotensin II-induced skeletal muscle atrophy. Life Sci, 2020, 250: 117593.
21. Kitajima Y, Yoshioka K, Suzuki N. The ubiquitin-proteasome system in regulation of the skeletal muscle homeostasis and atrophy: from basic science to disorders. J Physiol Sci, 2020, 70(1): 40.
22. Sartori R, Romanello V, Sandri M. Mechanisms of muscle atrophy and hypertrophy: implications in health and disease. Nat Commun, 2021, 12(1): 330.
23. Kobayashi H, Kato H, Hirabayashi Y, et al. Modulations of muscle protein metabolism by branched-chain amino acids in normal and muscle-atrophying rats. J Nutr, 2006, 136(1 Suppl): 234S-236S.
24. Børsheim E, Bui QU, Tissier S, et al. Effect of amino acid supplementation on muscle mass, strength and physical function in elderly. Clin Nutr, 2008, 27(2): 189-195.
25. Zhao Q, Shen H, Su KJ, et al. A joint analysis of metabolomic profiles associated with muscle mass and strength in Caucasian women. Aging (Albany NY), 2018, 10(10): 2624-2635.
26. Murphy RA, Moore SC, Playdon M, et al. Metabolites associated with lean mass and adiposity in older black men. J Gerontol A Biol Sci Med Sci, 2017, 72(10): 1352-1359.
27. 杨蕾. 中老年人肌肉减少症与代谢综合征的相关性及代谢组学研究. 乌鲁木齐: 新疆医科大学, 2020.
28. 鲁翰霖. 基于代谢组学的早期骨骼肌损伤时间推断的研究. 太原: 山西医科大学, 2019.

West China Medical Journal

Effect of corn oligopeptide on dexamethasone-induced muscle atrophy

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