EXPRESSIONS OF BONE MORPHOGENETIC PROTEIN 2 AND RUNT-RELATED TRANSCRIPTION FACTOR 2 AND MICROARCHITECTURE OF TRABECULAR BONE PERIACETABULA IN ADULT PATIENTS WITH DEVELOPMENTAL DYSPLASIA OF HIP_Chinese Journal of Reparative and Reconstructive Surgery

Authors：

LIU Ruiyu , WANG Kunzheng , LI Yongwei , BAI Chuanyi , WANG Chunsheng , DANG Xiaoqian.

Department of Orthopedics, Second Hospital Affiliated to the Medical College of Xi’an Jiaotong University, Xi’an Shaanxi, 710004, P.R.China. Corresponding author: WANG Kunzheng, E-mail: kunzheng1955@163.com;

Corresponding author：

Keywords：

Developmental dysplasia of the hip; Acetabular component loosening; Bone morphogenetic protein 2; Runt-related transcription factor 2; Microarchitecture

DOI：

10.7507/1002-1892.20130039

Video：

Export PDF Favorites Scan Get Citation

Abstract Full text Figures/Tables Video References Cited by

Objective To explore the expressions of bone morphogenetic protein 2 (BMP-2) and runt-related transcription facotr 2 (Runx2) and microarchitecture of trabecular bone periacetabula in adult patients with developmental dysplasia of the hip (DDH). Methods Between March and September 2008, the trabecular bone periacetabulum was collected from 8 patients with DDH who were scheduled for total hip arthroplasty (aged 37-55 years, 3 males and 5 females, trial group) and from 8 patients with avascular necrosis of the femoral head (Ficat stage II) who were scheduled for hip resurfacing arthroplasty (aged 36-55 years, 3 males and 5 females, control group). The expressions of BMP-2 and Runx2 in the trabecular bone were determined by real-time quantitative PCR, and the microarchitecture was observed by micro-CT and the following parameters were determined: bone volume/total volume (BV/TV), connectivity density (Conn.Dens), trabecular number (Tb. N), trabecular thickness (Tb.Th), trabecular separation (Tb.Sp), and structure model index (SMI). Results The expressions of BMP-2 and Runx2 were significantly lower in trial group than in control group (P lt; 0.05). The micro-CT showed sparse trabecular bone in trial group and dense trabecular bone in control group. BV/TV and Tb.N in trial group were significantly lower than those in control group, and SMI and Tb.Sp in trial group were significantly higher than those in control group (P lt; 0.05); there was no significant difference in Conn.Dens and Tb.Th between 2 groups (P gt; 0.05). Conclusion The trabecular bone is in a low metabolism condition and its microarchitecture is tendency to be osteoporosis trabecualr bone in adult patients with DDH. It may be related with the acetabular component loosening after total hip arthroplasty.

Citation： LIU Ruiyu,WANG Kunzheng,LI Yongwei,BAI Chuanyi,WANG Chunsheng,DANG Xiaoqian.. EXPRESSIONS OF BONE MORPHOGENETIC PROTEIN 2 AND RUNT-RELATED TRANSCRIPTION FACTOR 2 AND MICROARCHITECTURE OF TRABECULAR BONE PERIACETABULA IN ADULT PATIENTS WITH DEVELOPMENTAL DYSPLASIA OF HIP. Chinese Journal of Reparative and Reconstructive Surgery, 2013, 27(2): 173-177. doi: 10.7507/1002-1892.20130039 Copy

1.	Arabmotlagh M, Hennigs T, Warzecha J, et al. Bone strength influences periprosthetic bone loss after hip arthroplasty. Clin Orthop Relat Res, 2005, (440): 178-183.
2.	Kerner J, Huiskes R, van Lenthe GH, et al. Correlation between pre-operative periprosthetic bone density and post-operative bone loss in THA can be explained by strain-adaptive remodeling. J Biomech, 1999, 32(7): 695-703.
3.	Krischak GD, Wachter NJ, Zabel T, et al. Influence of preoperative mechanical bone quality and bone mineral density on aseptic loosening of total hip arthroplasty after seven years. Clin Biomech (Bristol, Avon), 2003, 18(10): 916-923.
4.	Jacobsen S, Rømer L, Søballe K. Degeneration in dysplastic hips. A computer tomography study. Skeletal Radiol, 2005, 34(12): 778-784.
5.	Crowe JF, Mani VJ, Ranawat CS. Total hip replacement in congenital dislocation and dysplasia of the hip. J Bone Joint Surg (Am), 1979, 61(1): 15-23.
6.	Clauss M, Ilchmann T, Zimmermann P, et al. Classification of acetabular changes in osteoarthritis: a histological and radiological analysis of 122 consecutive drill biopsies routinely taken during THA. Surg Radiol Anat, 2008, 30(7): 547-556.
7.	Kuliwaba JS, Fazzalari NL, Findlay DM. Stability of RNA isolated from human trabecular bone at post-mortem and surgery. Biochim Biophys Acta, 2005, 1740(1): 1-11.
8.	Fazzalari NL, Parkinson IH. Fractal properties of subchondral cancellous bone in severe osteoarthritis of the hip. J Bone Miner Res, 1997, 12(4): 632-640.
9.	Schnitzler CM, Mesquita JM, Wane L. Bone histomorphometry of the iliac crest, and spinal fracture prevalence in atrophic and hypertrophic osteoarthritis of the hip. Osteoporos Int, 1992, 2(4): 186-194.
10.	Amstutz HC, Ball ST, Le DuV MJ, et al. Resurfacing THA for patients younger than 50 years: results of 2- to 9-year follow up. Clin Orthop Relat Res, 2007, (460): 159-164.
11.	Onsten I, Akesson K, Obrant KJ. Micromotion of the acetabular component and periacetabular bone morphology. Clin Orthop Relat Res, 1995, (310): 103-110.
12.	Widmer KH, ZurXuh B, Morscher EW. Load transfer and fixation mode of press-fit acetabular sockets. J Arthroplasty, 2002, 17(7): 926-935.
13.	Kim YH, Kim JS. Histologic analysis of acetabular and proximal femoral bone in patients with osteonecrosis of the femoral head. J Bone Joint Surg (Am), 2004, 86-A(11): 2471-2474.
14.	Boden SD. The ABCs of BMPs. Orthop Nurs, 2005, 24(1): 49-52.
15.	Satija NK, Gurudutta GU, Sharma S, et al. Mesenchymal stem cells: molecular targets for tissue engineering. Stem Cells Dev, 2007, 16(1): 7-23.
16.	Karsenty G. Role of Cbfa1 in osteoblast differentiation and function. Semin Cell Dev Biol, 2000, 11(5): 343-346.
17.	Ducy P, Zhang R, Geoffroy V, et al. Osf2/Cbfa1: a transcriptional activator of osteoblast differentiation. Cell, 1997, 89(5): 747-754.
18.	Tsiridis E, Gamie Z, Conaghan PG. Biological options to enhance periprosthetic bone mass. Injury, 2007, 38(6): 704-713.
19.	Wilkinson JM, Eagleton AC, Stockley I, et al. Effect of pamidronate on bone turnover and implant migration after total hip arthroplasty: a randomized trial. J Orthop Res, 2005, 23(1): 1-8.
20.	Wilkinson JM, Stockley I, Peel NF, et al. Effect of pamidronate in preventing local bone loss after total hip arthroplasty: a randomized, double-blind, controlled trial. J Bone Miner Res, 2001, 16(3): 556-564.
21.	Friedl G, Radl R, Stihsen C, et al. The effect of a single infusion of zoledronic acid on early implant migration in total hip arthroplasty. A randomized, double-blind, controlled trial. J Bone Joint Surg (Am), 2009, 91(2): 274-281.

1. Arabmotlagh M, Hennigs T, Warzecha J, et al. Bone strength influences periprosthetic bone loss after hip arthroplasty. Clin Orthop Relat Res, 2005, (440): 178-183.
2. Kerner J, Huiskes R, van Lenthe GH, et al. Correlation between pre-operative periprosthetic bone density and post-operative bone loss in THA can be explained by strain-adaptive remodeling. J Biomech, 1999, 32(7): 695-703.
3. Krischak GD, Wachter NJ, Zabel T, et al. Influence of preoperative mechanical bone quality and bone mineral density on aseptic loosening of total hip arthroplasty after seven years. Clin Biomech (Bristol, Avon), 2003, 18(10): 916-923.
4. Jacobsen S, Rømer L, Søballe K. Degeneration in dysplastic hips. A computer tomography study. Skeletal Radiol, 2005, 34(12): 778-784.
5. Crowe JF, Mani VJ, Ranawat CS. Total hip replacement in congenital dislocation and dysplasia of the hip. J Bone Joint Surg (Am), 1979, 61(1): 15-23.
6. Clauss M, Ilchmann T, Zimmermann P, et al. Classification of acetabular changes in osteoarthritis: a histological and radiological analysis of 122 consecutive drill biopsies routinely taken during THA. Surg Radiol Anat, 2008, 30(7): 547-556.
7. Kuliwaba JS, Fazzalari NL, Findlay DM. Stability of RNA isolated from human trabecular bone at post-mortem and surgery. Biochim Biophys Acta, 2005, 1740(1): 1-11.
8. Fazzalari NL, Parkinson IH. Fractal properties of subchondral cancellous bone in severe osteoarthritis of the hip. J Bone Miner Res, 1997, 12(4): 632-640.
9. Schnitzler CM, Mesquita JM, Wane L. Bone histomorphometry of the iliac crest, and spinal fracture prevalence in atrophic and hypertrophic osteoarthritis of the hip. Osteoporos Int, 1992, 2(4): 186-194.
10. Amstutz HC, Ball ST, Le DuV MJ, et al. Resurfacing THA for patients younger than 50 years: results of 2- to 9-year follow up. Clin Orthop Relat Res, 2007, (460): 159-164.
11. Onsten I, Akesson K, Obrant KJ. Micromotion of the acetabular component and periacetabular bone morphology. Clin Orthop Relat Res, 1995, (310): 103-110.
12. Widmer KH, ZurXuh B, Morscher EW. Load transfer and fixation mode of press-fit acetabular sockets. J Arthroplasty, 2002, 17(7): 926-935.
13. Kim YH, Kim JS. Histologic analysis of acetabular and proximal femoral bone in patients with osteonecrosis of the femoral head. J Bone Joint Surg (Am), 2004, 86-A(11): 2471-2474.
14. Boden SD. The ABCs of BMPs. Orthop Nurs, 2005, 24(1): 49-52.
15. Satija NK, Gurudutta GU, Sharma S, et al. Mesenchymal stem cells: molecular targets for tissue engineering. Stem Cells Dev, 2007, 16(1): 7-23.
16. Karsenty G. Role of Cbfa1 in osteoblast differentiation and function. Semin Cell Dev Biol, 2000, 11(5): 343-346.
17. Ducy P, Zhang R, Geoffroy V, et al. Osf2/Cbfa1: a transcriptional activator of osteoblast differentiation. Cell, 1997, 89(5): 747-754.
18. Tsiridis E, Gamie Z, Conaghan PG. Biological options to enhance periprosthetic bone mass. Injury, 2007, 38(6): 704-713.
19. Wilkinson JM, Eagleton AC, Stockley I, et al. Effect of pamidronate on bone turnover and implant migration after total hip arthroplasty: a randomized trial. J Orthop Res, 2005, 23(1): 1-8.
20. Wilkinson JM, Stockley I, Peel NF, et al. Effect of pamidronate in preventing local bone loss after total hip arthroplasty: a randomized, double-blind, controlled trial. J Bone Miner Res, 2001, 16(3): 556-564.
21. Friedl G, Radl R, Stihsen C, et al. The effect of a single infusion of zoledronic acid on early implant migration in total hip arthroplasty. A randomized, double-blind, controlled trial. J Bone Joint Surg (Am), 2009, 91(2): 274-281.

Previous Article
SURGICAL TREATMENT OF CALCANEAL FRACTURE INVOLVING TALOCALCANEAL AND CALCANEOCUBOID JOINTS
Next Article
APPLICATION OF HIGH FREQUENCY COLOR DOPPLER ULTRASOUND IN ANTEROLATERAL THIGH FLAP SURGERY

Chinese Journal of Reparative and Reconstructive Surgery

EXPRESSIONS OF BONE MORPHOGENETIC PROTEIN 2 AND RUNT-RELATED TRANSCRIPTION FACTOR 2 AND MICROARCHITECTURE OF TRABECULAR BONE PERIACETABULA IN ADULT PATIENTS WITH DEVELOPMENTAL DYSPLASIA OF HIP

Abstract Full text Figures/Tables Video References Cited by

Previous Article

Next Article

Format

Content