EXPERIMENTAL STUDY ON QUICK PREPARATION OF OSTEOPOROSIS IN VITRO MODEL_Chinese Journal of Reparative and Reconstructive Surgery

Authors：

SHANG Qing ¹ , LIU Zongchao ² , LU Xiaobo ³

1Department of Orthopaedics, the People’s Hospital of Fushun, Fushun Sichuan, 643200, P.R.China;;
2Department of Orthopaedics, the Second Affiliated Hospital of Luzhou Medical College;;
3Department of Orthopaedics, the Affiliated Hospital of Luzhou Medical College. Corresponding author: LU Xiaobo, E-mail: luxiaobo1963@126.com;

Corresponding author：

Keywords：

Osteoporosis In vitro model; EDTA Quantitative computed tomography; Biomechanics; Goat

DOI：

10.7507/1002-1892.20130075

Video：

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Objective To establish a rapid, simple, and economic method to prepare osteoporosis (OP) in vitro model. Methods Eighty pairs of fresh goat femur were collected from 18-month-old female goats and were randomly divided into 4 groups (20 pairs in each group). The femur was immersed decalcifying solution (18% EDTA) for 1-5 days (group B), 6-10 days (group C), and 11-15 days (group D), while group A had no treatment as control. Four pairs of femur were taken out every day. Quantitative computed tomography was used to scan the medial and lateral femoral condyles, and the bone mineral density (BMD) was calculated. Electronic universal testing machine was used to do three-point bending test and compress and tensile ultimate strenght test, and the mechanical parameters for femur were calculated. Results With demineralized time passing, BMD of the medial and lateral femoral condyles were downtrend in groups A, B, C, and D, showing significant differences among 4 groups (P lt; 0.05); BMD of the lateral femoral condyle was significantly higher than that of the medial femoral condyle in each group (P lt; 0.05). The three-point bending test showed that broken load, ultimate strength, and elastic modulus of groups A and B were significantly higher than those of groups C and D (P lt; 0.05); but no significant difference was found between groups A and B, and between groups C and D (P gt; 0.05). Compress and tensile ultimate strength test showed that the compress and tensile ultimate strengths were significantly higher in group A than in groups C and D (P lt; 0.05), and in group B than in group D (P lt; 0.05), but no significant difference was found between groups A and B, between groups B and C, and between groups C and D (P gt; 0.05). Conclusion The 18% EDTA immersing for 6-15 days is a fast, simple, economical method to prepare an OP in vitro model of goat femur.

Citation： SHANG Qing,LIU Zongchao,LU Xiaobo. EXPERIMENTAL STUDY ON QUICK PREPARATION OF OSTEOPOROSIS IN VITRO MODEL. Chinese Journal of Reparative and Reconstructive Surgery, 2013, 27(3): 326-329. doi: 10.7507/1002-1892.20130075 Copy

1.	刘忠厚, 杨定焯, 朱汉民, 等. 中国人骨质疏松症建议诊断标准(第二稿). 中国骨质疏松杂志, 2000, 6(1): 1-3.
2.	Lozo P, Krpan D, Krvavica A, et al. Bone histology in postmenopausal osteoporosis variations in cellular activity. Act Med Croatica, 2004, 58(1): 5-11.
3.	薛延. 骨质疏松症的现状、流行趋势与研究方向. 中国全科医学, 2005, 8(16): 1299.
4.	王亮, 张志敏, 甄相周, 等. 去势雌性山羊骨质疏松模型的特点. 中国组织工程研究, 2012, 16(7): 1303-1306.
5.	张光, 孙晶, 韩邕, 等. 正常人群骨矿物含量的定量CT测量和研究. 中国医学影像技术, 1998, 14(6): 402-404.
6.	Kanis JA, Melton LJ, Christiansen C, et al. The diagnosis. J Bone Miner Res, 1994, 9(8): 1137-1141.
7.	李良, 陈槐卿, 陈孟诗, 等. 建立骨质疏松山羊模型初探. 中国骨质疏松杂志, 1998, 4(2): 12-16.
8.	李良, 陈槐卿, 陈孟诗, 等. 去卵巢山羊长骨生物力学性能的变化. 生物医学工程学杂志, 1998, 15(2): 101-105, 110.
9.	张智海, 沈建雄, 吴志宏, 等. 快速制备骨质疏松性椎体压缩骨折动物模型研究. 中国骨质疏松杂志, 2005, 11(1): 19-21, 51.
10.	张智海, 沈建雄, 吴志宏, 等. 骨质疏松性椎体压缩骨折动物模型短期制备方法初探. 美中国际创伤杂志, 2003, 2(2): 14-16, F003.
11.	杨彬奎, 雷伟, 王军, 等. 小牛椎体骨质疏松模型的快速建立. 第四军医大学学报, 2008, 29(12): 1061-1063.
12.	曹涌, 张烽, 姚羽, 等. 快速建立小牛椎体骨质疏松模型. 中国组织工程研究与临床康复, 2011, 15(39): 7288-7291.
13.	徐继红, 陈卫民. Th-EDTA络合滴定法测定羟乙膦酸二钠片含量. 解放军药学学报, 1999, 15(4): 56-57.
14.	刘大维, 初同伟, 张良, 等. EDTA微波脱钙骨的免疫组化染色. 第三军医大学学报, 2003, 25(1): 77-78.
15.	贾军宏, 刘忠厚. QCT与pQCT在骨质疏松诊断中的临床应用. 中国骨质疏松杂志, 2003, 9(2): 172-176.
16.	毛京沭, 杨波. 双能X线吸收法及定量CT法诊断骨质疏松症的比较研究. 中国骨质疏松杂志, 1997, 3(1): 33-35.
17.	赵亮, 李来, 黄敬, 等. 探讨应用QCT诊断骨质疏松症. 中国骨质疏松杂志, 2004, 10(3): 293-295.
18.	蒋建军, 杨定焯. 骨质量与骨密度影像学检查及评价. 医师进修杂志, 2005, 28(5): 6-9.
19.	褚保成, 江志勇. 定量CT在骨质疏松诊断中的应用. 中华外科杂志, 1992, 30(8): 467-469.
20.	李良, 吴文超, 陈槐卿, 等. 去势雌山羊骨组织形态学变化的动态观察. 华西医科大学学报, 1997, 28(4): 398-400.
21.	李良, 陈槐卿, 何成明, 等. 双侧卵巢切除山羊不同时间段骨形态计量学与骨密度的变化. 中国骨质疏松杂志, 2000, 6(2): 10-12.
22.	何成明, 陈槐卿, 李良, 等. 雌性山羊去卵巢后不同时间骨生物力学参数变化. 生物医学工程学杂志, 1999, 16(3): 295-299.

1. 刘忠厚, 杨定焯, 朱汉民, 等. 中国人骨质疏松症建议诊断标准(第二稿). 中国骨质疏松杂志, 2000, 6(1): 1-3.
2. Lozo P, Krpan D, Krvavica A, et al. Bone histology in postmenopausal osteoporosis variations in cellular activity. Act Med Croatica, 2004, 58(1): 5-11.
3. 薛延. 骨质疏松症的现状、流行趋势与研究方向. 中国全科医学, 2005, 8(16): 1299.
4. 王亮, 张志敏, 甄相周, 等. 去势雌性山羊骨质疏松模型的特点. 中国组织工程研究, 2012, 16(7): 1303-1306.
5. 张光, 孙晶, 韩邕, 等. 正常人群骨矿物含量的定量CT测量和研究. 中国医学影像技术, 1998, 14(6): 402-404.
6. Kanis JA, Melton LJ, Christiansen C, et al. The diagnosis. J Bone Miner Res, 1994, 9(8): 1137-1141.
7. 李良, 陈槐卿, 陈孟诗, 等. 建立骨质疏松山羊模型初探. 中国骨质疏松杂志, 1998, 4(2): 12-16.
8. 李良, 陈槐卿, 陈孟诗, 等. 去卵巢山羊长骨生物力学性能的变化. 生物医学工程学杂志, 1998, 15(2): 101-105, 110.
9. 张智海, 沈建雄, 吴志宏, 等. 快速制备骨质疏松性椎体压缩骨折动物模型研究. 中国骨质疏松杂志, 2005, 11(1): 19-21, 51.
10. 张智海, 沈建雄, 吴志宏, 等. 骨质疏松性椎体压缩骨折动物模型短期制备方法初探. 美中国际创伤杂志, 2003, 2(2): 14-16, F003.
11. 杨彬奎, 雷伟, 王军, 等. 小牛椎体骨质疏松模型的快速建立. 第四军医大学学报, 2008, 29(12): 1061-1063.
12. 曹涌, 张烽, 姚羽, 等. 快速建立小牛椎体骨质疏松模型. 中国组织工程研究与临床康复, 2011, 15(39): 7288-7291.
13. 徐继红, 陈卫民. Th-EDTA络合滴定法测定羟乙膦酸二钠片含量. 解放军药学学报, 1999, 15(4): 56-57.
14. 刘大维, 初同伟, 张良, 等. EDTA微波脱钙骨的免疫组化染色. 第三军医大学学报, 2003, 25(1): 77-78.
15. 贾军宏, 刘忠厚. QCT与pQCT在骨质疏松诊断中的临床应用. 中国骨质疏松杂志, 2003, 9(2): 172-176.
16. 毛京沭, 杨波. 双能X线吸收法及定量CT法诊断骨质疏松症的比较研究. 中国骨质疏松杂志, 1997, 3(1): 33-35.
17. 赵亮, 李来, 黄敬, 等. 探讨应用QCT诊断骨质疏松症. 中国骨质疏松杂志, 2004, 10(3): 293-295.
18. 蒋建军, 杨定焯. 骨质量与骨密度影像学检查及评价. 医师进修杂志, 2005, 28(5): 6-9.
19. 褚保成, 江志勇. 定量CT在骨质疏松诊断中的应用. 中华外科杂志, 1992, 30(8): 467-469.
20. 李良, 吴文超, 陈槐卿, 等. 去势雌山羊骨组织形态学变化的动态观察. 华西医科大学学报, 1997, 28(4): 398-400.
21. 李良, 陈槐卿, 何成明, 等. 双侧卵巢切除山羊不同时间段骨形态计量学与骨密度的变化. 中国骨质疏松杂志, 2000, 6(2): 10-12.
22. 何成明, 陈槐卿, 李良, 等. 雌性山羊去卵巢后不同时间骨生物力学参数变化. 生物医学工程学杂志, 1999, 16(3): 295-299.

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Chinese Journal of Reparative and Reconstructive Surgery

EXPERIMENTAL STUDY ON QUICK PREPARATION OF OSTEOPOROSIS IN VITRO MODEL

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