MECHANISM OF VASCULAR STROMAL FRACTION AT EARLY STAGE AFTER ASPIRATED FAT TRANSPLANTATION_Chinese Journal of Reparative and Reconstructive Surgery

Authors：

YUAN Yi , LU Feng , GAO Jianhua.

Department of Plastic Surgery, Nanfang Hospital of Southern Medical University, Guangzhou Guangdong, 510515, P.R.China. Corresponding author: GAO Jianhua, E-mail: gaopsnf@yahoo.com.cn;

Corresponding author：

Keywords：

Vascular stromal fraction; Aspirated fat transplantation; Angiogenesis; Growth factor Nude mouse

DOI：

10.7507/1002-1892.20130103

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Objective To investigate the mechanism of vascular stromal fraction (SVF) at the early stage after aspirated fat transplantation. Methods Fat was harvested from 5 cases of women undergoing abdominal liposuction operation, and SVF was isolated. Aspirated fat with (group B) or without (group A) SVF was injected subcutaneously into the back of nude mice, and the grafts were harvested at 1, 3, 5, and 7 days. Graft wet weight was measured; and immunohistochemical method (CD31) was performed and the secretion of vascular endothelial growth factor (VEGF) and hepatocyte growth factor (HGF) were qnantified by Western blot assay. Results The wet weight of transplanted adipose tissue showed an increasing tendency in groups A and B with time, and no significant difference was found between groups A and B (P gt; 0.05). At 1 and 3 days after transplantation, no CD31 positive cells was seen in 2 groups; the CD31 positive cells of group B were significantly more than those of group A at 5 and 7 days (P lt; 0.05), and the CD31 positive cells at 7 days were significantly more than those at 5 days in 2 groups (P lt; 0.05). Western blot test showed that VEGF expression reached peak at 3 days , then decreased gradually; the expression of VEGF protein in group B was significantly higher than that in group A at 1, 3, and 5 days (P lt; 0.05). The expression of HGF protein in groups A and B remained at a high level within 5 days, but it tended to decrease at 7 days, which was significantly higher in group B than that in group A (P lt; 0.05). Conclusion SVF can enhance angiogenesis by secretion of growth factors at the early stage after aspirated fat transplantation.

Citation： YUAN Yi,LU Feng,GAO Jianhua.. MECHANISM OF VASCULAR STROMAL FRACTION AT EARLY STAGE AFTER ASPIRATED FAT TRANSPLANTATION. Chinese Journal of Reparative and Reconstructive Surgery, 2013, 27(4): 449-453. doi: 10.7507/1002-1892.20130103 Copy

1.	Risau W. Mechanisms of angiogenesis. Nature, 1997, 386(6626): 671-674.
2.	Yamaguchi T, Sawa Y, Miyamoto Y, et al. Therapeutic angiogenesis induced by injecting hepatocyte growth factor in ischemic canine hearts. Surg Today, 2005, 35(10): 855-860.
3.	Miyagawa S, Sawa Y, Fukuda K, et al. Angiogenic gene cell therapy using suicide gene system regulates the effect of angiogenesis in infarcted rat heart. Transplantation, 2006, 81(6): 902-907.
4.	Taniyama Y, Morishita R, Aoki M, et al. Therapeutic angiogenesis induced by human hepatocyte growth factor gene in rat and rabbit hindlimb ischemia models: preclinical study for treatment of peripheral arterial disease. Gene Ther, 2001, 8(3): 181-189.
5.	Yoshimura K, Shigeura T, Matsumoto D, et al. Characterization of freshly isolated and cultured cells derived from the fatty and fluid portions of liposuction aspirates. J Cell Physiol, 2006, 208(1): 64-76.
6.	Matsumoto D, Sato K, Gonda K, et al. Cell-assisted lipotransfer: Supportive use of human adipose-derived cells for soft tissue augmentation with lipoinjection. Tissue Eng, 2006, 12(12): 3375-3382.
7.	Yoshimura K, Sato K, Aoi N, et al. Cell-assisted lipotransfer for facial lipoatrophy: efficacy of clinical use of adipose-derived stem cells. Dermatol Surg, 2008, 34(9): 1178-1185.
8.	Zuk PA, Zhu M, Ashjian P, et al. Human adipose tissue is a source of multipotent stem cells. Mol Biol Cell, 2002, 13(12): 4279-4295.
9.	Tallone T, Realini C, Bohmler A, et al. Adult human adipose tissue contains several types of multipotent cells. J Cardiovasc Transl Res, 2011, 4(2): 200-210.
10.	Wilson A, Butler PE, Seifalian AM. Adipose-derived stem cells for clinical applications: a review. Cell Prolif, 2011, 44(1): 86-98.
11.	Shiffman MA, Mirrafati S. Fat transfer techniques: the effect of harvest and transfer methods on adipocyte viability and review of the literature. Dermatol Surg, 2001, 27(9): 819-826.
12.	Christiaens V, Lijnen HR. Angiogenesis and development of adipose tissue. Mol Cell Endocrinol, 2010, 318(1-2): 2-9.
13.	Yoshimura K, Suga H, Eto H. Adipose-derived stem/progenitor cells: roles in adipose tissue remodeling and potential use for soft tissue augmentation. Regen Med, 2009, 4(2): 265-273.
14.	Lee EY, Xia Y, Kim WS, et al. Hypoxia-enhanced wound-healing function of adipose-derived stem cells: increase in stem cell proliferation and up-regulation of VEGF and bFGF. Wound Repair Regen, 2009, 17(4): 540-547.
15.	Rubina K, Kalinina N, Efimenko A, et al. Adipose stromal cells stimulate angiogenesis via promoting progenitor cell differentiation, secretion of angiogenic factors, and enhancing vessel maturation. Tissue Eng Part A, 2009, 15(8): 2039-2050.
16.	Eto H, Kato H, Suga H, et al. The fate of adipocytes after nonvascularized fat grafting: evidence of early death and replacement of adipocytes. Plast Reconstr Surg, 2012, 129(5): 1081-1092.
17.	Maumus M, Peyrafitte JA, D’Angelo R, et al. Native human adipose stromal cells: localization, morphology and phenotype. Int J Obes (Lond), 2011, 35(9): 1141-1153.
18.	Traktuev DO, Prater DN, Merfeld-Clauss S, et al. Robust functional vascular network formation in vivo by cooperation of adipose progenitor and endothelial cells. Circ Res, 2009, 104(12): 1410-1420.
19.	Butala P, Hazen A, Szpalski C, et al. Endogenous stem cell therapy enhances fat graft survival. Plast Reconstr Surg, 2012, 130(2): 293-306.

1. Risau W. Mechanisms of angiogenesis. Nature, 1997, 386(6626): 671-674.
2. Yamaguchi T, Sawa Y, Miyamoto Y, et al. Therapeutic angiogenesis induced by injecting hepatocyte growth factor in ischemic canine hearts. Surg Today, 2005, 35(10): 855-860.
3. Miyagawa S, Sawa Y, Fukuda K, et al. Angiogenic gene cell therapy using suicide gene system regulates the effect of angiogenesis in infarcted rat heart. Transplantation, 2006, 81(6): 902-907.
4. Taniyama Y, Morishita R, Aoki M, et al. Therapeutic angiogenesis induced by human hepatocyte growth factor gene in rat and rabbit hindlimb ischemia models: preclinical study for treatment of peripheral arterial disease. Gene Ther, 2001, 8(3): 181-189.
5. Yoshimura K, Shigeura T, Matsumoto D, et al. Characterization of freshly isolated and cultured cells derived from the fatty and fluid portions of liposuction aspirates. J Cell Physiol, 2006, 208(1): 64-76.
6. Matsumoto D, Sato K, Gonda K, et al. Cell-assisted lipotransfer: Supportive use of human adipose-derived cells for soft tissue augmentation with lipoinjection. Tissue Eng, 2006, 12(12): 3375-3382.
7. Yoshimura K, Sato K, Aoi N, et al. Cell-assisted lipotransfer for facial lipoatrophy: efficacy of clinical use of adipose-derived stem cells. Dermatol Surg, 2008, 34(9): 1178-1185.
8. Zuk PA, Zhu M, Ashjian P, et al. Human adipose tissue is a source of multipotent stem cells. Mol Biol Cell, 2002, 13(12): 4279-4295.
9. Tallone T, Realini C, Bohmler A, et al. Adult human adipose tissue contains several types of multipotent cells. J Cardiovasc Transl Res, 2011, 4(2): 200-210.
10. Wilson A, Butler PE, Seifalian AM. Adipose-derived stem cells for clinical applications: a review. Cell Prolif, 2011, 44(1): 86-98.
11. Shiffman MA, Mirrafati S. Fat transfer techniques: the effect of harvest and transfer methods on adipocyte viability and review of the literature. Dermatol Surg, 2001, 27(9): 819-826.
12. Christiaens V, Lijnen HR. Angiogenesis and development of adipose tissue. Mol Cell Endocrinol, 2010, 318(1-2): 2-9.
13. Yoshimura K, Suga H, Eto H. Adipose-derived stem/progenitor cells: roles in adipose tissue remodeling and potential use for soft tissue augmentation. Regen Med, 2009, 4(2): 265-273.
14. Lee EY, Xia Y, Kim WS, et al. Hypoxia-enhanced wound-healing function of adipose-derived stem cells: increase in stem cell proliferation and up-regulation of VEGF and bFGF. Wound Repair Regen, 2009, 17(4): 540-547.
15. Rubina K, Kalinina N, Efimenko A, et al. Adipose stromal cells stimulate angiogenesis via promoting progenitor cell differentiation, secretion of angiogenic factors, and enhancing vessel maturation. Tissue Eng Part A, 2009, 15(8): 2039-2050.
16. Eto H, Kato H, Suga H, et al. The fate of adipocytes after nonvascularized fat grafting: evidence of early death and replacement of adipocytes. Plast Reconstr Surg, 2012, 129(5): 1081-1092.
17. Maumus M, Peyrafitte JA, D’Angelo R, et al. Native human adipose stromal cells: localization, morphology and phenotype. Int J Obes (Lond), 2011, 35(9): 1141-1153.
18. Traktuev DO, Prater DN, Merfeld-Clauss S, et al. Robust functional vascular network formation in vivo by cooperation of adipose progenitor and endothelial cells. Circ Res, 2009, 104(12): 1410-1420.
19. Butala P, Hazen A, Szpalski C, et al. Endogenous stem cell therapy enhances fat graft survival. Plast Reconstr Surg, 2012, 130(2): 293-306.

Chinese Journal of Reparative and Reconstructive Surgery

MECHANISM OF VASCULAR STROMAL FRACTION AT EARLY STAGE AFTER ASPIRATED FAT TRANSPLANTATION

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