EXPERIMENTAL STUDY ON HEMOSTASIS OF THERMOSENSITIVE CHITOSAN HEMOSTATIC FILM_Chinese Journal of Reparative and Reconstructive Surgery

Authors：

YIN Gang ¹ , WEI Changzheng ² , GUO Xingfeng ¹ , CHEN Huihao ¹ , HOU Chunlin ¹

1Department of Orthopaedics, Shanghai Changzheng Hospital, Shanghai, 200003, P.R.China;;
2Shanghai Qisheng Biological Preparation Co., Ltd. Corresponding author: HOU Chunlin, E-mail: chunlin_hou@yahoo.com.cn;

Corresponding author：

Keywords：

Thermosensitive chitosan; Biological hemostatic material; Rat

DOI：

10.7507/1002-1892.20130137

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Objective To investigate the hemostasis of thermosensitive chitosan hemostatic film. Methods Fifty adult Sprague Dawley rats, male or female and weighing 190-210 g, were made the models of liver injury. The models were randomly divided into 5 groups (n=10) depending on different hemostatic materials. The incision of the liver was covered with the hemostatic materials of 2.0 cm × 1.0 cm × 0.5 cm in size: thermosensitive chitosan hemostatic film (group A), chitosan hemostatic film (group B), cellulose hemostatic cotton (group C), gelatin sponge (group D), and no treatment (group E), respectively. The bleeding time and bleeding amount were recorded. After 4 weeks, the incisions of the liver were observed with HE staining. Results Gross observation showed better hemostatic effect and faster hemostatic time in groups A, B, and C; group D had weaker hemostatic effect and slower hemostatic time; group E had no hemostatic effect. The bleeding time and bleeding amount of groups A, B, C, and D were significantly lower than those of group E (P lt; 0.05). The bleeding time and bleeding amount of groups A, B, and C were significantly lower than those of group D (P lt; 0.05), but no significant difference was found among groups A, B, and C (P gt; 0.05). The liver cells of group A had milder edema and ballooning degeneration than other 4 groups through histological observation. Conclusion The thermosensitive chitosan hemostatic film has good hemostasis effect on the liver incision of rats.

Citation： YIN Gang,WEI Changzheng,GUO Xingfeng,CHEN Huihao,HOU Chunlin. EXPERIMENTAL STUDY ON HEMOSTASIS OF THERMOSENSITIVE CHITOSAN HEMOSTATIC FILM. Chinese Journal of Reparative and Reconstructive Surgery, 2013, 27(5): 624-627. doi: 10.7507/1002-1892.20130137 Copy

1.	侯春林, 顾其胜. 几丁质与医学. 上海: 上海科学技术出版社, 2001: 65-69.
2.	Muzzarelli RA, Muzzarelli C. Chitosan chemistry: relevance to the biomedical sciences. Advances in Polymer Science, 2005, 186: 151-209.
3.	Kumar MN, Muzzarelli RA, Muzzarelli C, et al. Chitosan chemistry and pharmaceutical perspectives. Chem Rev, 2004, 104(12): 6017-6084.
4.	曾春, 蔡道章, 全大萍. 壳聚糖在软骨组织工程中的应用. 中国修复重建外科杂志, 2005, 19(10): 831-834.
5.	尹刚, 侯春林, 刘万顺, 等. 新型壳聚糖止血粉止血作用的实验研究. 中国修复重建外科杂志, 2009, 23(11): 1347-1349.
6.	Jackson MR, Taher MM, Burge JR, et al. Hemostatic efficacy of a fibrin sealant dressing in an animal model of kidney injury. J Trauma, 1998, 45(4): 662-665.
7.	王春, 谷天祥, 喻磊, 等. 再生氧化纤维素在严重骨质疏松体外循环手术胸骨止血中的作用. 中国组织工程研究与临床康复, 2011, 15(25): 4739-4742.
8.	谢霞, 唐杰. 局部止血剂的临床研究新进展. 中国药物应用与监测, 2011, 8(5): 311-314.
9.	于美丽, 杜智. 可吸收止血材料的研究现状及临床应用. 北京生物医学工程, 2008, 27(2): 208-211.
10.	Jamborova G, Pospisilova N, Semecky V, et al. Microdispersed Oxidized Cellulose as a novel potential substance with hypolipidemic properties. Nutrition, 2008, 24(11-12): 1174-1181.
11.	Ishihara M, Nakanishi K, Ono K, et al. Photocrosslinkable chitosan as a dressing for wound occlusion and accelerator in healing process. Biomaterials, 2002, 23(3): 833-840.
12.	Martinowitz U, Spotnitz WD. Fibrin tissue adhesives. Thromb Haemost, 1997, 78(1): 661-666.
13.	斯清庆, 侯春林, 蒋丽霞, 等. 几丁糖膜的制备及其止血性能的研究. 解放军医学杂志, 2002, 27(12): 1055-1056.
14.	刘亚平, 侯春林, 顾其胜, 等. 可生物降解止血粉的制备及其止血性能. 中国修复重建外科杂志, 2007, 21(8): 829-832.
15.	Malette WG, Quigleg HJ, Gaines RD, et al．Chitosan: a new hemostatic. Ann Thorac Surg, 1983, 36(1): 55-58.
16.	Pusateri AE, McCarthy SJ, Gregory KW, et al. Effect of a chitosan-based hemostatic dressing on blood loss and survival in a model of severe venous hemorrhage and hepatic injury in swine. J Trauma, 2003, 54(1): 177-182.
17.	Wei CZ, Hou CL, Gu QS, et al. A thermosensitive chitosan-based hydrogel barrier for post-operative adhesions’ prevention. Biomaterials, 2009, 30(29): 5534-5540.

1. 侯春林, 顾其胜. 几丁质与医学. 上海: 上海科学技术出版社, 2001: 65-69.
2. Muzzarelli RA, Muzzarelli C. Chitosan chemistry: relevance to the biomedical sciences. Advances in Polymer Science, 2005, 186: 151-209.
3. Kumar MN, Muzzarelli RA, Muzzarelli C, et al. Chitosan chemistry and pharmaceutical perspectives. Chem Rev, 2004, 104(12): 6017-6084.
4. 曾春, 蔡道章, 全大萍. 壳聚糖在软骨组织工程中的应用. 中国修复重建外科杂志, 2005, 19(10): 831-834.
5. 尹刚, 侯春林, 刘万顺, 等. 新型壳聚糖止血粉止血作用的实验研究. 中国修复重建外科杂志, 2009, 23(11): 1347-1349.
6. Jackson MR, Taher MM, Burge JR, et al. Hemostatic efficacy of a fibrin sealant dressing in an animal model of kidney injury. J Trauma, 1998, 45(4): 662-665.
7. 王春, 谷天祥, 喻磊, 等. 再生氧化纤维素在严重骨质疏松体外循环手术胸骨止血中的作用. 中国组织工程研究与临床康复, 2011, 15(25): 4739-4742.
8. 谢霞, 唐杰. 局部止血剂的临床研究新进展. 中国药物应用与监测, 2011, 8(5): 311-314.
9. 于美丽, 杜智. 可吸收止血材料的研究现状及临床应用. 北京生物医学工程, 2008, 27(2): 208-211.
10. Jamborova G, Pospisilova N, Semecky V, et al. Microdispersed Oxidized Cellulose as a novel potential substance with hypolipidemic properties. Nutrition, 2008, 24(11-12): 1174-1181.
11. Ishihara M, Nakanishi K, Ono K, et al. Photocrosslinkable chitosan as a dressing for wound occlusion and accelerator in healing process. Biomaterials, 2002, 23(3): 833-840.
12. Martinowitz U, Spotnitz WD. Fibrin tissue adhesives. Thromb Haemost, 1997, 78(1): 661-666.
13. 斯清庆, 侯春林, 蒋丽霞, 等. 几丁糖膜的制备及其止血性能的研究. 解放军医学杂志, 2002, 27(12): 1055-1056.
14. 刘亚平, 侯春林, 顾其胜, 等. 可生物降解止血粉的制备及其止血性能. 中国修复重建外科杂志, 2007, 21(8): 829-832.
15. Malette WG, Quigleg HJ, Gaines RD, et al．Chitosan: a new hemostatic. Ann Thorac Surg, 1983, 36(1): 55-58.
16. Pusateri AE, McCarthy SJ, Gregory KW, et al. Effect of a chitosan-based hemostatic dressing on blood loss and survival in a model of severe venous hemorrhage and hepatic injury in swine. J Trauma, 2003, 54(1): 177-182.
17. Wei CZ, Hou CL, Gu QS, et al. A thermosensitive chitosan-based hydrogel barrier for post-operative adhesions’ prevention. Biomaterials, 2009, 30(29): 5534-5540.

Chinese Journal of Reparative and Reconstructive Surgery

EXPERIMENTAL STUDY ON HEMOSTASIS OF THERMOSENSITIVE CHITOSAN HEMOSTATIC FILM

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