EXPERIMENTAL STUDY ON TREATMENT OF LATERAL FEMORAL CONDYLE FRACTURE WITH POLYLACTIC-CO-GLYCOLIC ACID/HYDROXYAPATITE ABSORBABLE CANNULATED SCREWS IN CANINE_Chinese Journal of Reparative and Reconstructive Surgery

Authors：

ZHENG Guo ¹ , LIU Dong ² , TANG Xin ¹ , LI Qi ¹ , LI Jian ¹ , LI Qing ³ , XIAO Jianping ³

1Department of Orthopaedics, West China Hospital, Sichuan University, Chengdu Sichuan, 610041, P.R.China;;
2Department of Orthopaedics, Suining Central Hospital;;
3Chengdu Organic Chemicals Co., Ltd. Chinese Academy of Sciences. Corresponding author: LI Jian, E-mail: hxlijian.china@163.com;

Corresponding author：

Keywords：

Polylactic-co-glycolic acid/hydroxyapatite; Absorbable cannulated screw; Lateral femoral condyle fracture; Internal fixation; Biodegradation; Canine

DOI：

10.7507/1002-1892.20130151

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Objective To evaluate the internal fixation effect, degradation, and biocompatibility of polylactic-co-glycolic acid/hydroxyapatite (PLGA/HA) absorbable cannulated screws in treatment of lateral femoral condyle fracture of canine so as to provide the theory basis for their further improvement and clinical application. Methods Sixteen adult male Beagles (weighing, 9-12 kg) were selected to prepare the models of bilateral lateral femoral condyle fracture; left fracture was fixed with PLGA/HA absorbable cannulated screws as experimental group and right fracture with metal screws as control group. At 2, 4, 8, and 12 weeks after operation, general observation was done and X-ray films were taken for observing fracture healing; bone mineral density was measured; the histological examination was performed; and the degradation property of absorbable cannulated screws was detected. Results All animals survived to the end of the experiment. General observations showed that no fracture displacement occurred and fracture healed at 12 weeks in 2 groups; no breakage, displacement, or loosening of screws was observed in experimental group. X-ray films results showed that the absorbable cannulated screws could not be found out by X-ray in experimental group, but metal screws could be found out in control group; fracture healed with time in 2 groups. The bone mineral density reached the peak at 8 weeks in 2 groups, and no significant difference was found between 2 groups and among different time points in the same group (P gt; 0.05). Histological examination showed that 2 groups had similar fracture healing process at different time points; no obvious inflammatory reaction was found around absorbable cannulated screws in experimental group. The degradation results of absorbable cannulated screws showed that the intrinsic viscosity and molecular weight distribution obviously decreased at 2 weeks; the number average molecular weight and the weight average molecular weight markedly decreased at 4 weeks; and the maximum shear force did not decrease obviously at 8 weeks, and then decreased significantly. Significant differences were found in all indexes among different time points in the same group (P lt; 0.05). Conclusion PLGA/HA absorbable cannulated screws and metal screws show similar fracture healing process for fixing lateral femoral condyle fracture of canine, and the absorbable canulated screws have good biocompatibility. The maximum shear force of PLGA/HA absorbable cannulated screw has no obvious decrease during 8 weeks after operation, so it can ensure full healing of fracture.

Citation： ZHENG Guo,LIU Dong,TANG Xin,LI Qi,LI Jian,LI Qing,XIAO Jianping. EXPERIMENTAL STUDY ON TREATMENT OF LATERAL FEMORAL CONDYLE FRACTURE WITH POLYLACTIC-CO-GLYCOLIC ACID/HYDROXYAPATITE ABSORBABLE CANNULATED SCREWS IN CANINE. Chinese Journal of Reparative and Reconstructive Surgery, 2013, 27(6): 680-685. doi: 10.7507/1002-1892.20130151 Copy

1.	Jayabalan M, Shalumon KT, Mitha MK, et al. Effect of hydroxyapatite on the biodegradation and biomechanical stability of polyester nanocomposites for orthopaedic applications. Acta Biomaterialia J, 2010, 6(3): 763-775.
2.	白雁斌, 黄晓琴, 雷自强. 聚乳酸类医用生物降解材料的研究进展. 高分子通报, 2006, (3): 46-51.
3.	Cordewener FW, Bos RR, Rozema FR, et al. Poly (L-lactide) implants for repair of human orbital floor defects: clinical and magnetic resonance Imaging evaluation of long-term results. J Oral Maxillofac Surg, 1996, 54(1): 9-13.
4.	李庆, 庞秀炳, 蒋柳云, 等. 可吸收骨内植入材料及其制备方法: 中国, CN102085397A. 2011-06-08.
5.	Friend G, Grace K, Stone HA. L-osteotomy with absorbable fixation for correction of tailor’s bunion. J Foot Ankle Surg, 1993, 32(1): 14-19.
6.	徐又佳, 郑祖根, 王晓东, 等. 可吸收内固定材科对实验性骨折愈合影响的超微结构观察. 中华骨科杂志, 1996, 16(10): 653-655.
7.	黄福龙, 戴红莲, 方园, 等. HA/PDLLA复合材料的制备及其降解性能研究. 无机材料学报, 2007, 22(2): 333-337.
8.	Fan Y, Xiu K, Duan H, et al. Biomechanical and histological evalution of the application of biodegradable poly-L-lactic cushion to the plate internal fixation for bone fracture healing. Clin Biomech (Bristol, Avon), 2008, 23 Suppl 1: S7-S16.
9.	Hasegawa S, Neo M, Tamura J, et al. In vivo evaluation of a porous hydroxyaptite poly-DL-lactide composite for bone tissue engineering. J Biomed Mater Res A, 2007, 81(4): 930-938.
10.	Hasegawa S, Tamura J, Neo M, et al. In vivo evaluation of a porous hydroxyapatite poly-DL-laetide composite for use as a bone substitute. J Biomed Mate Res A, 2005, 75(3): 567-579.
11.	赵建华, 廖维宏, 王远亮, 等. 消旋聚乳酸/轻基磷灰石/脱钙骨基质的制备及其体外降解特性研究. 中国修复重建外科杂志, 2003, 17(1): 61-64.
12.	Dong J, Kojima H, Uemura T, et al. In vivo evaluation of a novel porou hydroxyapatite to sustain osteogenesis of transplanted bone marrow-derived osteoblastic cells. J Biomed Mater Res, 2001, 57(2): 208-216.
13.	Hoffman GO. Biodegradable implants in traumatology: a review on the state-of-the-art. Arch Orthop Trauma Surg, 1995, 114(3): 123-132.
14.	Hoffmann JF. Update on biodegradable plating systems: theory and results. Otolaryngol Head Neck Surg, 2000, 8(4): 288-293.
15.	Ashammakhi N, Suuronen R, Tiainen J, et al. Spotlight on naturally absorbable osteofixation devices. J Craniofacial Surg, 2003, 14(2): 247-259.
16.	Palmer RH. Biological osteosynthesis. Vet Clin North Am Small Anim Pract, 1999, 29(5): 1171-1185.
17.	Cotton NJ, Egan MJ, Brunelle JE. Composites of poly (DL-lactide-co-glycolide) and calcium carbonate: in vitro evaluation for use in orthopedic applications. J Biomed Mater Res A, 2008, 85(1): 195-205.
18.	Matsusue Y, Yamamuro T, Oka M, et al. In vitro and in vivo studies on bioabsorbable ultra-high-strength poly (L-lactide) rods. J Biomed Mater Res, 1992, 26(12): 1553-1567.
19.	Mackool R, Yim J, McCarthy JG. Delayed degradation in a resorbable plating system. J Craniofacial Surg, 2006, 17(1): 197-198.
20.	Yerit KC, Hainich S, Turhani D, et al. Stability of biodegradable implants in treatment of mandibular fractures. Plast Reconstr Surg, 2005, 115(7): 1863-1870.
21.	Spoliti M. Glenoid osteolysis after arthroseopie labrum repair with a bioabsorbable suture anchor. Acta Orthop Belg, 2007, 73(1): 107-110.
22.	Böstman OM. Osteoarthritis of the ankle after foreign-body reaction to absorbable pins and screws: a three-to nine-year follow-up study. J Bone Joint Surg (Br), 1998, 80(2): 333-338.
23.	Mukherjee DP, Pietrzak WS. Bioabsorbable fixation: scientific, technical, and clinical concepts. J Craniofac Surg, 2011, 22(2): 679-689.
24.	Pietrzak WS, Sarver DR, Verstynen ML. Bioabsorbable polymer science for the practicing surgeon. J Craniofac Surg, 1997, 8(2): 87-91.

1. Jayabalan M, Shalumon KT, Mitha MK, et al. Effect of hydroxyapatite on the biodegradation and biomechanical stability of polyester nanocomposites for orthopaedic applications. Acta Biomaterialia J, 2010, 6(3): 763-775.
2. 白雁斌, 黄晓琴, 雷自强. 聚乳酸类医用生物降解材料的研究进展. 高分子通报, 2006, (3): 46-51.
3. Cordewener FW, Bos RR, Rozema FR, et al. Poly (L-lactide) implants for repair of human orbital floor defects: clinical and magnetic resonance Imaging evaluation of long-term results. J Oral Maxillofac Surg, 1996, 54(1): 9-13.
4. 李庆, 庞秀炳, 蒋柳云, 等. 可吸收骨内植入材料及其制备方法: 中国, CN102085397A. 2011-06-08.
5. Friend G, Grace K, Stone HA. L-osteotomy with absorbable fixation for correction of tailor’s bunion. J Foot Ankle Surg, 1993, 32(1): 14-19.
6. 徐又佳, 郑祖根, 王晓东, 等. 可吸收内固定材科对实验性骨折愈合影响的超微结构观察. 中华骨科杂志, 1996, 16(10): 653-655.
7. 黄福龙, 戴红莲, 方园, 等. HA/PDLLA复合材料的制备及其降解性能研究. 无机材料学报, 2007, 22(2): 333-337.
8. Fan Y, Xiu K, Duan H, et al. Biomechanical and histological evalution of the application of biodegradable poly-L-lactic cushion to the plate internal fixation for bone fracture healing. Clin Biomech (Bristol, Avon), 2008, 23 Suppl 1: S7-S16.
9. Hasegawa S, Neo M, Tamura J, et al. In vivo evaluation of a porous hydroxyaptite poly-DL-lactide composite for bone tissue engineering. J Biomed Mater Res A, 2007, 81(4): 930-938.
10. Hasegawa S, Tamura J, Neo M, et al. In vivo evaluation of a porous hydroxyapatite poly-DL-laetide composite for use as a bone substitute. J Biomed Mate Res A, 2005, 75(3): 567-579.
11. 赵建华, 廖维宏, 王远亮, 等. 消旋聚乳酸/轻基磷灰石/脱钙骨基质的制备及其体外降解特性研究. 中国修复重建外科杂志, 2003, 17(1): 61-64.
12. Dong J, Kojima H, Uemura T, et al. In vivo evaluation of a novel porou hydroxyapatite to sustain osteogenesis of transplanted bone marrow-derived osteoblastic cells. J Biomed Mater Res, 2001, 57(2): 208-216.
13. Hoffman GO. Biodegradable implants in traumatology: a review on the state-of-the-art. Arch Orthop Trauma Surg, 1995, 114(3): 123-132.
14. Hoffmann JF. Update on biodegradable plating systems: theory and results. Otolaryngol Head Neck Surg, 2000, 8(4): 288-293.
15. Ashammakhi N, Suuronen R, Tiainen J, et al. Spotlight on naturally absorbable osteofixation devices. J Craniofacial Surg, 2003, 14(2): 247-259.
16. Palmer RH. Biological osteosynthesis. Vet Clin North Am Small Anim Pract, 1999, 29(5): 1171-1185.
17. Cotton NJ, Egan MJ, Brunelle JE. Composites of poly (DL-lactide-co-glycolide) and calcium carbonate: in vitro evaluation for use in orthopedic applications. J Biomed Mater Res A, 2008, 85(1): 195-205.
18. Matsusue Y, Yamamuro T, Oka M, et al. In vitro and in vivo studies on bioabsorbable ultra-high-strength poly (L-lactide) rods. J Biomed Mater Res, 1992, 26(12): 1553-1567.
19. Mackool R, Yim J, McCarthy JG. Delayed degradation in a resorbable plating system. J Craniofacial Surg, 2006, 17(1): 197-198.
20. Yerit KC, Hainich S, Turhani D, et al. Stability of biodegradable implants in treatment of mandibular fractures. Plast Reconstr Surg, 2005, 115(7): 1863-1870.
21. Spoliti M. Glenoid osteolysis after arthroseopie labrum repair with a bioabsorbable suture anchor. Acta Orthop Belg, 2007, 73(1): 107-110.
22. Böstman OM. Osteoarthritis of the ankle after foreign-body reaction to absorbable pins and screws: a three-to nine-year follow-up study. J Bone Joint Surg (Br), 1998, 80(2): 333-338.
23. Mukherjee DP, Pietrzak WS. Bioabsorbable fixation: scientific, technical, and clinical concepts. J Craniofac Surg, 2011, 22(2): 679-689.
24. Pietrzak WS, Sarver DR, Verstynen ML. Bioabsorbable polymer science for the practicing surgeon. J Craniofac Surg, 1997, 8(2): 87-91.

Chinese Journal of Reparative and Reconstructive Surgery

EXPERIMENTAL STUDY ON TREATMENT OF LATERAL FEMORAL CONDYLE FRACTURE WITH POLYLACTIC-CO-GLYCOLIC ACID/HYDROXYAPATITE ABSORBABLE CANNULATED SCREWS IN CANINE

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