PRIMARY STUDY ON TISSUE ENGINEERED PERIOSTEUM OSTEOGENESIS TO REPAIR SCAPULA DEFECT IN VIVO IN ALLOGENIC RABBIT_Chinese Journal of Reparative and Reconstructive Surgery

Authors：

ZHANGCangyu , WANGShuanke , RENGuangtie , TUOZhenhe , YUJiajia , WANGJing , ANLiping , MAJinglin ,  ZHAOLin

Department of Orthopedics, the 2nd Hospital of Lanzhou University, the Bone and Joint Key Laboratory of Gansu Province, Lanzhou Gansu, 730030, P. R. China;

Corresponding author：

ZHAOLin, Email: bonezl@sina.com

Keywords：

Tissue engineered periosteum; Bone marrow mesenchymal stem cells; Small intestinal submucosa; Bone defect repair; Rabbit; Pig

DOI：

10.7507/1002-1892.20140085

Video：

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Objective To investigate the feasibility of tissue engineered periosteum (TEP) constructed by porcine small intestinal submucosa (SIS) and bone marrow mesenchymal stem cells (BMSCs) of rabbit to repair the large irregular bone defects in allogenic rabbits. Methods The BMSCs were cultivated from the bone marrow of New Zealand white rabbits (aged, 2 weeks-1 month). SIS was fabricated by porcine proximal jejunum. The TEP constructed by SIS scaffold and BMSCs was prepared in vitro. Eighteen 6-month-old New Zealand white rabbits whose scapula was incompletely resected to establish one side large irregular bone defects (3 cm×3 cm) model. The bone defects were repaired with TEP (experimental group,n=9) and SIS (control group,n=9), respectively. At 8 weeks after operation, the rabbits were sacrificed, and the implants were harvested. The general condition of the rabbits was observed; X-ray radiography and score according to Lane-Sandhu criteria, and histological examination (HE staining and Masson staining) were performed. Results After operation, all animals had normal behavior and diet; the incision healed normally. The X-ray results showed new bone formation with normal bone density in the defect area of experimental group; but no bone formation was observed in control group. The X-ray score was 6.67±0.32 in experimental group and was 0.32±0.04 in control group, showing significant difference (t=19.871,P=0.001). The general observation of the specimens showed bone healing at both ends of the defect, and the defect was filled by new bone in experimental group; no new bone formed in the control group. The histological staining showed new bone tissue where there were a lot of new vessels and medullary cavity, and no macrophages or lymphocytes infiltration was observed in the defect area of experimental group; only some connective tissue was found in the control group. Conclusion TEP constructed by porcine SIS and BMSCs of rabbit can form new bone in allogenic rabbit and has the feasibility to repair the large irregular bone defects.

Citation： ZHANGCangyu, WANGShuanke, RENGuangtie, TUOZhenhe, YUJiajia, WANGJing, ANLiping, MAJinglin, ZHAOLin. PRIMARY STUDY ON TISSUE ENGINEERED PERIOSTEUM OSTEOGENESIS TO REPAIR SCAPULA DEFECT IN VIVO IN ALLOGENIC RABBIT. Chinese Journal of Reparative and Reconstructive Surgery, 2014, 28(3): 384-388. doi: 10.7507/1002-1892.20140085 Copy

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15.	Zhao L, Zhao JL, Wan L, et al. The study of the feasibility of segmental bone defect repair with tissue-engineered bone membrane:a qualitative observation. Strategies Trauma Limb Reconstr, 2008, 3(2):57-64.
16.	李伟. 肩胛骨的临床解剖学研究. 广州:南方医科大学, 2007.
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18.	Hodde J, Janis A, Hiles M. Effects of sterilization on an extracellular matrix scaffold:part II. Bioactivity and matrix interaction. J Mater Sci Mater Med, 2007, 18(4):545-550.
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21.	Ye H, Das DB, Triffitt JT, et al. Modelling nutrient transport in hollow fibre membrane bioreactors for growing three-dimensional bone tissue. J Membrane Sci, 2006, 272(1-2):169-178.
22.	罗静聪, 杨志明. 小肠黏膜下层的制备及其特性的研究进展. 中国修复重建外科杂志, 2003, 17(5):425-428.

1. Ikada Y. Challenges in tissue engineering. J R Soc Interface, 2006, 3(10):589-601.
2. Augustin G, Antabak A, Davila S. The periosteum. Part 1:Anatomy, histology and molecular biology. Injury, 2007, 38(10):1115-1130.
3. Dwek JR. The periosteum:what is it, where is it, and what mimics it in its absence? Skeletal Radiol, 2010, 39(4):319-323.
4. Mackie EJ, Ahmed YA, Tatarczuch L, et al. Endochondral ossification:how cartilage is converted into bone in the developing skeleton. Int J Biochem Cell Biol, 2008, 40(1):46-62.
5. Hall BK, Miyake T. All for one and one for all:condensations and the initiation of skeletal development. Bioessays, 2000, 22(2):138-147.
6. 张大伟, 田清业, 刘光军, 等. 骨膜瓣复合异体骨移植修复大段骨缺损. 组织工程与重建外科, 2012, 8(1):26-31.
7. Mizuno H, Hata KI, Kojima K, et al. A novel approach to regenerating periodontal tissue by grafting autologous cultured periosteum. Tissue Eng, 2006, 12(5):1227-1335.
8. Zhao L, Zhao J, Wang S, et al. Comparative study between tissue-engineered periosteum and structural allograft in rabbit critical-sized radial defect model. J Biomed Mater Res B Appl Biomater, 2011, 97(1):1-9.
9. 庾佳佳, 汪新柱, 赵琳, 等. 兔骨髓间充质干细胞的分离培养及成骨诱导. 中国组织工程研究, 2013, 17(6):974-979.
10. Gersbach CA, Le Doux JM, Guldberg RE, et al. Inducible regulation of Runx2-stimulated osteogenesis. Gene Ther, 2006, 13(11):873-882.
11. Tang YL, Zhao Q, Zhang YC, et al. Autologous mesenchymal stem cell transplantation induce VEGF and neovascularization in ischemic myocardium. Regul Pept, 2004, 117(1):3-10.
12. Abraham GA, Murray J, Billiar K, et al. Evaluation of the porcine intestinal collagen layer as a biomaterial. J Biomed Mater Res, 2000, 51(3):442-452.
13. Xiao ZM, Jiang H, Zhan XL, et al. Treatment of osteonecrosis of femoral head with BMSCs-seeded bio-derived bone materials combined with rhBMP-2 in rabbits. Chin J Traumatol, 2008, 11(3):165-170.
14. 赵琳, 史志勇, 周晟, 等. 组织工程骨膜异体体内成骨修复兔骨缺损的初步观察. 中国修复重建外科杂志, 2008, 22(2):145-147.
15. Zhao L, Zhao JL, Wan L, et al. The study of the feasibility of segmental bone defect repair with tissue-engineered bone membrane:a qualitative observation. Strategies Trauma Limb Reconstr, 2008, 3(2):57-64.
16. 李伟. 肩胛骨的临床解剖学研究. 广州:南方医科大学, 2007.
17. Meirelles Lda S, Fontes AM, Covas DT, et al. Mechanisms involved in the therapeutic properties of mesenchymal stem cells. Cytokine Growth Factor Rev, 2009, 20(5):419-427.
18. Hodde J, Janis A, Hiles M. Effects of sterilization on an extracellular matrix scaffold:part II. Bioactivity and matrix interaction. J Mater Sci Mater Med, 2007, 18(4):545-550.
19. Ye H, Xia Z, Ferguson DJ, et al. Studies on the use of hollow fibre membrane bioreactors for tissue generation by using rat bone marrow fibroblastic cells and a composite scaffold. J Mater Sci Mater Med, 2007, 18(4):641-648.
20. 裴国献, 魏宽海. 显微外科与血管化组织工程组织的构建. 中华创伤骨科杂志, 2004, 6(4):361-363.
21. Ye H, Das DB, Triffitt JT, et al. Modelling nutrient transport in hollow fibre membrane bioreactors for growing three-dimensional bone tissue. J Membrane Sci, 2006, 272(1-2):169-178.
22. 罗静聪, 杨志明. 小肠黏膜下层的制备及其特性的研究进展. 中国修复重建外科杂志, 2003, 17(5):425-428.

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Chinese Journal of Reparative and Reconstructive Surgery

PRIMARY STUDY ON TISSUE ENGINEERED PERIOSTEUM OSTEOGENESIS TO REPAIR SCAPULA DEFECT IN VIVO IN ALLOGENIC RABBIT

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