REPAIR OF LARGE SEGMENTAL BONE DEFECT BY TISSUE ENGINEERED PERIOSTEUM AND DEPROTEINIZED BONE SCAFFOLD IN RABBITS_Chinese Journal of Reparative and Reconstructive Surgery

Authors：

TUOZhenhe ¹ ,  ZHAOLin ¹ , WANGShuanke ¹ , LUYongting ² , RENGuangtie ¹ , ZHANGCangyu ¹ , YUJiajia ¹ , SUNRui ¹ , WANGJing ¹

1. Department of Orthopaedics, Orthopaedics Key Laboratory of Gansu Province, the Second Hospital of Lanzhou University, Lanzhou Gansu, 730000, P. R. China;
2. Department of Gynecology, the Second Hospital of Lanzhou University, Lanzhou Gansu, 730000, P. R. China;

Corresponding author：

ZHAOLin, Email: bonezl@sina.com

Keywords：

Bone tissue engineering; Tissue engineered periosteum; Small intestinal submucosa; Bone marrow mesenchymal stem cells; Deproteinized bone; Rabbit

DOI：

10.7507/1002-1892.20140115

Video：

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Objective To evaluate the effect of tissue engineered periosteum on the repair of large diaphysis defect in rabbit radius, and the effect of deproteinized bone (DPB) as supporting scaffolds of tissue engineering periosteum. Methods Bone marrow mesenchymal stem cells (BMSCs) were cultured from 1-month-old New Zealand Rabbit and osteogenetically induced into osteoblasts. Porcine small intestinal submucosa (SIS) scaffold was produced by decellular and a series mechanical and physiochemical procedures. Then tissue engineered periosteum was constructed by combining osteogenic BMSCs and SIS, and then the adhesion of cells to scaffolds was observed by scanning electron microscope (SEM). Fresh allogeneic bone was drilled and deproteinized as DPB scaffold. Tissue engineered periosteum/DPB complex was constructed by tissue engineered periosteum and DPB. Tissue engineered periosteum was "coat-like" package the DPB, and bundled with absorbable sutures. Forty-eight New Zealand white rabbits (4-month-old) were randomly divided into 4 groups (groups A, B, C, and D, n=12). The bone defect model of 3.5 cm in length in the left radius was created. Defect was repaired with tissue engineered periosteum in group A, with DPB in group B, with tissue engineered periosteum/DPB in group C; defect was untreated in group D. At 4, 8, and 12 weeks after operation, 4 rabbits in each group were observed by X-ray. At 8 weeks after operation, 4 rabbits of each group were randomly sacrificed for histological examination. Results SEM observation showed that abundant seeding cells adhered to tissue engineered periosteum. At 4, 8, and 12 weeks after operation, X-ray films showed the newly formed bone was much more in groups A and C than groups B and D. The X-ray film score were significantly higher in groups A and C than in groups B and D, in group A than in group C, and in group B than in group D (P<0.05). Histological staining indicated that there was a lot of newly formed bone in the defect space in group A, with abundant newly formed vessels and medullary cavity. While in group B, the defect space filled with the DPB, the degradation of DPB was not obvious. In group C, there was a lot of newly formed bone in the defect space, island-like DPB and obvious DPB degradation were seen in newly formed bone. In group D, the defect space only replaced by some connective tissue. Conclusion Tissue engineered periosteum constructed by SIS and BMSCs has the feasibility to repair the large diaphysis defect in rabbit. DPB isn't an ideal support scaffold of tissue engineering periosteum, the supporting scaffolds of tissue engineered periosteum need further exploration.

Citation： TUOZhenhe, ZHAOLin, WANGShuanke, LUYongting, RENGuangtie, ZHANGCangyu, YUJiajia, SUNRui, WANGJing. REPAIR OF LARGE SEGMENTAL BONE DEFECT BY TISSUE ENGINEERED PERIOSTEUM AND DEPROTEINIZED BONE SCAFFOLD IN RABBITS. Chinese Journal of Reparative and Reconstructive Surgery, 2014, 28(4): 511-516. doi: 10.7507/1002-1892.20140115 Copy

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7.	李卫国, 黄向红, 唐景清, 等. 骨膜成骨细胞培养活体自体、异体和异种移植成骨的实验研究. 昆明医学院学报, 2008, 29(1):81-84.
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9.	吴伟炽, 黄东, 刘晓春, 等. 穿支骨膜瓣游离移植治疗难治性骨不连的疗效观察. 现代医院, 2013, 13(3):39-41.
10.	赵琳, 史志勇, 周晟, 等. 组织工程骨膜异体体内成骨修复兔骨缺损的初步观察. 中国修复重建外科杂志, 2008, 22(2):145-147.
11.	Muschler GF, Nakamoto C, Griffith LG. Engineering principles of clinical cell-based tissue engineering. J Bone Joint Surg (Am), 2004, 86-A(7):1541-1558.
12.	王金成, 李志洲, 陈伟, 等. 骨库骨的生物力学特性比较研究. 骨与关节损伤杂志, 2001, 16(1):35-36.
13.	简月奎, 罗阳, 田晓滨, 等. 改良法制备异种脱蛋白骨体内植入修复骨缺损的免疫学分析. 中国组织工程研究与临床康复, 2010, 14(42):7802-7806.

1. 许建中. 骨组织工程相关研究新进展. 中国修复重建外科杂志, 2010, 24(7):769-773.
2. Zhao L, Zhao JL, Wan L, et al. The study of the feasibility of segmental bone defect repair with tissue-engineered bone membrane:a qualitative observation. Strategies Trauma Limb Reconstr, 2008, 3(2):57-64.
3. 杨志明. 组织工程. 北京:化学工业出版社, 2002:225-227.
4. 范伟, 安洪, 蒋电明, 等. 脱蛋白骨为支架材料体外构建的组织工程骨. 中华医学杂志, 2006, 86(47):3349-3352.
5. Lane JM, Sandhu HS. Current approaches to experimental bone grafting. Orthop Clin North Am, 1987, 18(2):213-225.
6. 贝抗胜, 吴礼杨, 孙庆文, 等. 人骨膜细胞生物学特性的实验研究. 中华创伤骨科杂志, 2011, 13(12):1170-1174.
7. 李卫国, 黄向红, 唐景清, 等. 骨膜成骨细胞培养活体自体、异体和异种移植成骨的实验研究. 昆明医学院学报, 2008, 29(1):81-84.
8. Soldado F, Fontecha CG, Barber I, et al. Vascularized fibular periosteal graft:a new technique to enhance bone union in children. J Pediatr Orthop, 2012, 32(3):308-313.
9. 吴伟炽, 黄东, 刘晓春, 等. 穿支骨膜瓣游离移植治疗难治性骨不连的疗效观察. 现代医院, 2013, 13(3):39-41.
10. 赵琳, 史志勇, 周晟, 等. 组织工程骨膜异体体内成骨修复兔骨缺损的初步观察. 中国修复重建外科杂志, 2008, 22(2):145-147.
11. Muschler GF, Nakamoto C, Griffith LG. Engineering principles of clinical cell-based tissue engineering. J Bone Joint Surg (Am), 2004, 86-A(7):1541-1558.
12. 王金成, 李志洲, 陈伟, 等. 骨库骨的生物力学特性比较研究. 骨与关节损伤杂志, 2001, 16(1):35-36.
13. 简月奎, 罗阳, 田晓滨, 等. 改良法制备异种脱蛋白骨体内植入修复骨缺损的免疫学分析. 中国组织工程研究与临床康复, 2010, 14(42):7802-7806.

Chinese Journal of Reparative and Reconstructive Surgery

REPAIR OF LARGE SEGMENTAL BONE DEFECT BY TISSUE ENGINEERED PERIOSTEUM AND DEPROTEINIZED BONE SCAFFOLD IN RABBITS

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