IMMUNOGENICITY OF ALLOGENEIC FREEZING PERIOSTEUM AND BONE MARROW_Chinese Journal of Reparative and Reconstructive Surgery

Authors：

DENGJiuzheng ¹ ,  ZHANGYoule ² , LIXiang ³

1. Jishuitan College of Orthopedic Surgery, Medical Center of Tsinghua University, Beijing, 100091, P. R. China;
2. Department of Hand Surgery, Beijing Jishuitan Hospital;
3. Department of Orthopaedics and Traumatology, Li Ka Shing Faculty of Medicine, the University of Hong Kong, Hong Kong;

Corresponding author：

ZHANGYoule, Email: zhangyoule2007@hotmail.com

Keywords：

Allograft; Immunogenicity; Periosteum; Bone marrow; Rabbit

DOI：

10.7507/1002-1892.20140143

Video：

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Objective To investigate the immunogenicity of freezing periosteum and bone marrow during allogeneic joint transplantation, and to explore proper pretreatment of allogeneic joint. Methods The allogeneic periosteum and bone marrow were harvested from knee joints of 5 New Zealand white rabbits (aged, 6 months; weighing, 2.6-3.0 kg). After gradient cooling, the tissue was cryopreserved for 1 month. The freezing periosteum and bone marrow were grinded to pieces after rewarming to prepare the suspension of periosteum and bone marrow. Eighteen Chinchilla rabbits (aged, 6 months; weighing, 2.1-2.8 kg) were divided into 3 groups randomly:normal saline injection group (group A, n=6), periosteum injection group (group B, n=6), and bone marrow injection group (group C, n=6). The normal saline, periosteum suspension, and bone marrow suspension were injected into the peritoneal cavity in groups A, B, and C, respectively. The concentrations of interleukin 2 (IL-2), IL-6, and tumor necrosis factor α (TNF-α) in serum and the ratio of CD4⁺ T cell/CD8⁺ T cell in venous blood were measured before injection, at 1 week and 2 weeks after injection. Results There was no significant difference in the concentration of IL-2 between before and after injection in the same group (P=0.241), and between groups (P=0.055). The concentration of IL-6 after injection was significantly lower than that before injection in the same group (P=0.040), but no significant difference was found between groups (P=0.357). The concentration of TNF-α showed no significant difference between before and after injection in the same group (P=0.925), but the concentration of TNF-α in group B was significantly higher than that in groups A and C (P<0.05). The ratio of CD4⁺T cell/CD8⁺T cell of venous blood had no significant difference between before and after operation in the same group (P=0.248), and between groups (P=0.646). Conclusion The freezing periosteum and bone marrow are lowly immunogenic. In order to decrease the immunogenicity of the joint, preserving the periosteum and removing the marrow cavity are recommended.

Citation： DENGJiuzheng, ZHANGYoule, LIXiang. IMMUNOGENICITY OF ALLOGENEIC FREEZING PERIOSTEUM AND BONE MARROW. Chinese Journal of Reparative and Reconstructive Surgery, 2014, 28(5): 649-653. doi: 10.7507/1002-1892.20140143 Copy

1.	Lexer E. Free transplantation. Ann Surg, 1914, 60(2):166-194.
2.	Hofmann GO, Kirschner MH, Wagner FD, et al. Allogeneic vascularized transplantation of human femoral diaphyses and total knee joints-first clinical experiences. Transplant Proc, 1998, 30(6):2754-2761.
3.	孙和炎, 卜海富, 王明丽, 等. 软骨细胞培养及其冻存复苏后的生物学特性比较. 安徽医科大学学报, 2003, 38(2):107-109.
4.	文雪平, 张朝跃. 同种异体半关节移植治疗下肢关节端骨肿瘤. 现代生物医学进展, 2010, 10(13):2476-2478.
5.	Allieu Y, Marck G, Chammas M, et al. Total elbow joint allograft for long term posttraumatic osteoarticular loss. Follow-up results at twelve years. Rev Chir Orthop Reparatrice Appar Mot, 2004, 90(4):319-328.
6.	刘希云, 周从豪, 娄铁尘, 等. 冷冻异体骨膜引导骨组织再生的实验研究. 中华口腔医学杂志, 2000, 35(5):385-387, I026.
7.	许永涛, 顾洁夫, 尚平. 深低温保存的同种异体骨膜与胎骨联合移植的实验研究. 中国修复重建外科杂志, 2001, 15(3):183-187.
8.	张勇. 超深低温条件下较长冷冻时间对异体神经移植免疫排斥的影响. 泸州:泸州医学院, 2012.
9.	Minami A, An KN, Cooney WP 3rd, et al. Ligament stability of the metacarpophalangeal joint:a biomechanical study. J Hand Surg (Am), 1985, 10(2):255-260.
10.	郑振辉, 周淑佩, 彭双清. 实用医学实验动物学. 北京:北京大学医学出版社, 2008:161-165.
11.	范学敏, 李鹏翠, 丁娟, 等. 玻璃化冻存对兔关节软骨细胞存活率及生物学活性的影响. 中国骨科临床与基础研究杂志, 2012, 4(4):275-280.
12.	韩云, 刘军, 蓝妮, 等. 大鼠深低温冻储同种异体气管移植的实验研究. 中国医科大学学报, 2011, 40(12):1079-1080, 1084.
13.	Lechler RI, Garden OA, Turka LA. The complementary roles of deletion and regulation in transplantation tolerance. Nat Rev Immunol, 2003, 3(2):147-158.
14.	朱志刚, 曹建民. 氩氦刀冷冻消融术对荷瘤兔T细胞免疫的近期影响. 介入放射学杂志, 2010, 19(7):558-559.
15.	沈勇, 孔荣, 朱六龙. 兔组织工程化骨异体植入修复骨缺损中外周血T淋巴细胞亚群的检测. 安徽医科大学学报, 2006, 41(4):392-395.
16.	宋红星, 曹峻岭, 刘淼, 等. 同种异体软骨细胞移植修复兔膝关节软骨缺损的免疫反应. 中国矫形外科杂志, 2002, 9(1):32-34.
17.	张罗生, 罗荣城, 郑航, 等. RFA治疗对VX2肝肿瘤兔免疫功能影响的实验研究. 临床肿瘤学杂志, 2002, 7(6):401-404.
18.	Zhao L, Zhao J, Wang S, et al. Evaluation of immunocompatibility of tissue-engineered periosteum. Biomed Meter, 2011, 6(1):015005.
19.	Czitrom AA, Axelrod T, Fernandes B. Antigen presenting cells and bone allotransplantation. Clin Orthop Relat Res, 1985, (197):27-31.
20.	马以勇, 亓建洪. 冷冻保存对同种异体移植物免疫原性的影响. 泰山医学院学报, 2007, 28(5):397-400.
21.	刘斌. 组织学与胚胎学. 北京:北京大学医学出版社, 2003:67-68.
22.	Gotfried Y, Yaremchuk MJ, Randolph MA, et al. Histological characteristics of acute rejection in vascularized allografts of bone. J Bone Joint Surg (Am), 1987, 69(3):410-425.
23.	魏玲玲, 邓绍平, 黄孝伦. 肝细胞移植治疗急性肝功能衰竭的免疫排斥研究. 中国普外基础与临床杂志, 2012, 19(2):135-139.
24.	谢迎宾, 王强, 雷宁玉, 等. 兔角膜移植后房水中血管内皮生长因子及肿瘤坏死因子α表达与高压氧的影响. 中国组织工程研究, 2012, 16(31):5763-5767.
25.	杨涛, 杨勇, 刘斌, 等. 兔异体脂肪干细胞移植的实验研究. 现代生物医学进展, 2013, 13(7):1244-1248.

1. Lexer E. Free transplantation. Ann Surg, 1914, 60(2):166-194.
2. Hofmann GO, Kirschner MH, Wagner FD, et al. Allogeneic vascularized transplantation of human femoral diaphyses and total knee joints-first clinical experiences. Transplant Proc, 1998, 30(6):2754-2761.
3. 孙和炎, 卜海富, 王明丽, 等. 软骨细胞培养及其冻存复苏后的生物学特性比较. 安徽医科大学学报, 2003, 38(2):107-109.
4. 文雪平, 张朝跃. 同种异体半关节移植治疗下肢关节端骨肿瘤. 现代生物医学进展, 2010, 10(13):2476-2478.
5. Allieu Y, Marck G, Chammas M, et al. Total elbow joint allograft for long term posttraumatic osteoarticular loss. Follow-up results at twelve years. Rev Chir Orthop Reparatrice Appar Mot, 2004, 90(4):319-328.
6. 刘希云, 周从豪, 娄铁尘, 等. 冷冻异体骨膜引导骨组织再生的实验研究. 中华口腔医学杂志, 2000, 35(5):385-387, I026.
7. 许永涛, 顾洁夫, 尚平. 深低温保存的同种异体骨膜与胎骨联合移植的实验研究. 中国修复重建外科杂志, 2001, 15(3):183-187.
8. 张勇. 超深低温条件下较长冷冻时间对异体神经移植免疫排斥的影响. 泸州:泸州医学院, 2012.
9. Minami A, An KN, Cooney WP 3rd, et al. Ligament stability of the metacarpophalangeal joint:a biomechanical study. J Hand Surg (Am), 1985, 10(2):255-260.
10. 郑振辉, 周淑佩, 彭双清. 实用医学实验动物学. 北京:北京大学医学出版社, 2008:161-165.
11. 范学敏, 李鹏翠, 丁娟, 等. 玻璃化冻存对兔关节软骨细胞存活率及生物学活性的影响. 中国骨科临床与基础研究杂志, 2012, 4(4):275-280.
12. 韩云, 刘军, 蓝妮, 等. 大鼠深低温冻储同种异体气管移植的实验研究. 中国医科大学学报, 2011, 40(12):1079-1080, 1084.
13. Lechler RI, Garden OA, Turka LA. The complementary roles of deletion and regulation in transplantation tolerance. Nat Rev Immunol, 2003, 3(2):147-158.
14. 朱志刚, 曹建民. 氩氦刀冷冻消融术对荷瘤兔T细胞免疫的近期影响. 介入放射学杂志, 2010, 19(7):558-559.
15. 沈勇, 孔荣, 朱六龙. 兔组织工程化骨异体植入修复骨缺损中外周血T淋巴细胞亚群的检测. 安徽医科大学学报, 2006, 41(4):392-395.
16. 宋红星, 曹峻岭, 刘淼, 等. 同种异体软骨细胞移植修复兔膝关节软骨缺损的免疫反应. 中国矫形外科杂志, 2002, 9(1):32-34.
17. 张罗生, 罗荣城, 郑航, 等. RFA治疗对VX2肝肿瘤兔免疫功能影响的实验研究. 临床肿瘤学杂志, 2002, 7(6):401-404.
18. Zhao L, Zhao J, Wang S, et al. Evaluation of immunocompatibility of tissue-engineered periosteum. Biomed Meter, 2011, 6(1):015005.
19. Czitrom AA, Axelrod T, Fernandes B. Antigen presenting cells and bone allotransplantation. Clin Orthop Relat Res, 1985, (197):27-31.
20. 马以勇, 亓建洪. 冷冻保存对同种异体移植物免疫原性的影响. 泰山医学院学报, 2007, 28(5):397-400.
21. 刘斌. 组织学与胚胎学. 北京:北京大学医学出版社, 2003:67-68.
22. Gotfried Y, Yaremchuk MJ, Randolph MA, et al. Histological characteristics of acute rejection in vascularized allografts of bone. J Bone Joint Surg (Am), 1987, 69(3):410-425.
23. 魏玲玲, 邓绍平, 黄孝伦. 肝细胞移植治疗急性肝功能衰竭的免疫排斥研究. 中国普外基础与临床杂志, 2012, 19(2):135-139.
24. 谢迎宾, 王强, 雷宁玉, 等. 兔角膜移植后房水中血管内皮生长因子及肿瘤坏死因子α表达与高压氧的影响. 中国组织工程研究, 2012, 16(31):5763-5767.
25. 杨涛, 杨勇, 刘斌, 等. 兔异体脂肪干细胞移植的实验研究. 现代生物医学进展, 2013, 13(7):1244-1248.

Chinese Journal of Reparative and Reconstructive Surgery

IMMUNOGENICITY OF ALLOGENEIC FREEZING PERIOSTEUM AND BONE MARROW

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