PRELIMINARY HISTOLOGICAL OBSERVATION OF EFFECT OF EXPANDED CAPSULE ON COSTAL CARTILAGE AUTOGRAFT_Chinese Journal of Reparative and Reconstructive Surgery

Authors：

SHANGQiaoli ,  JIANGHaiyue , ZHOUJiayu , HELeren , YANGQinghua

The 7th Department of Plastic Surgery, Plastic Surgery Hospital, Chinese Academy of Medical Sciences, Peking Union Medical College, Beijing, 100144, P. R. China;

Corresponding author：

JIANGHaiyue, Email: jianghaiyue_psh@163.com

Keywords：

Costal cartilage autograft; Expanded capsule; Histological observation; Rabbit

DOI：

10.7507/1002-1892.20140165

Video：

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Objective To explore the effect of the expanded capsule on the growth of autogenous costal cartilage. Methods Sixteen New Zealand white rabbits at the age of 3 months (weighing, 2.2-2.5 kg; male or female) were selected and four 15 mL tissue expanders were implanted on the back symmetrically. After 1 month, the expanded capsule formed, the tissue expanders were removed; the capsule of the left side was removed (experimental group), and the capsule of the right side was reserved (control group); meanwhile, the right 7th and 8th costal cartilage without the perichondrium was divided into segments and placed into the capsule of 2 groups symmetrically. At 4 and 8 weeks after transplantation, the cartilage was harvested for the general, weighing, and histological observations. Results One rabbit died during the experiment, and the other 15 rabbits survived. The differences of cartilage weight between before and after transplantation showed more obvious increase in the experimental group[(0.003 4±0.002 7) g and (0.005 8±0.001 4) g] than those in the control group[(-0.000 3±0.001 9) g and (-0.003 9±0.005 3) g] at 4 and 8 weeks, showing significant differences between 2 gouprs (t=4.331, P=0.029; t=6.688, P=0.008). The change of cartilage weight at 8 weeks was significantly higher than that at 4 weeks in the experimental group (t=-3.098, P=0.001); but the change of cartilage weight at 8 weeks was significantly lower than that at 4 weeks in the control group (t=2.491, P=0.009). The histological observation showed that the activity of the cartilage was enhanced in 2 groups at 4 and 8 weeks when compared with normal cartilage, and more obvious change was observed in the experimental group than in the control group. And the acellular area was seen in the cartilage at 8 weeks in the control group. The Masson staining results showed that the color was deeper in the experimental group than in the control group. Conclusion The removal of the expanded capsule during operation is beneficial to the growth of autogenous costal cartilage. The results can provide corresponding experimental guidance for the clinical problems.

Citation： SHANGQiaoli, JIANGHaiyue, ZHOUJiayu, HELeren, YANGQinghua. PRELIMINARY HISTOLOGICAL OBSERVATION OF EFFECT OF EXPANDED CAPSULE ON COSTAL CARTILAGE AUTOGRAFT. Chinese Journal of Reparative and Reconstructive Surgery, 2014, 28(6): 741-744. doi: 10.7507/1002-1892.20140165 Copy

1.	庄洪兴, 蒋海越, 潘博, 等. 先天性小耳畸形的皮肤软组织扩张器法外耳再造术. 中华整形外科杂志, 2006, 22(4):286-289.
2.	Kerem M, Orbay H, Sensöz O. Use of an expander temporoparietal fascial flap tecnique for total auricular reconstruction. Plast Reconstr Surg, 2007, 119(7):2320-2321.
3.	Dashan Y, Haiyue J, Qinghua Y, et al. Technical innovations in ear reconstruction using a skin expander with autogenous cartilage grafts. Plast Reconstr Aesthet Surg, 2008, 61 Suppl 1:S59-69.
4.	Jiang H, Pan B, Lin L, et al. Ten-year experience in microtia reconstruction using expander and autogenous cartilage. Int J Pediatr Otorhinolaryngol, 2008, 72(8):1251-1259.
5.	Brent B. Technical advances in ear reconstruction with autogenous rib cartilage grafts:personal experience with 1200 cases. Plast Reconstr Surg, 1999, 104(2):319-338.
6.	Kajin N, Asatoh H. Reconstruction of the auricle with autogeneous rib cartilage grafts. Adv Otorhinolaryngol, 2014, 75:61-75.
7.	Sivayoham E, Woolford TJ. Current opinion on auricular reconstruction. Curr Opin Otolaryngol Head Neck Surg, 2012, 20(4):287-290.
8.	Qing-Hua Y, Yu-Peng S, Haiyue J, et al. The significance of the biomechanical properties of costal cartilage in the timing of ear reconstruction surgery. Plast Reconstr Aesthet Surg, 2011, 64(6):742-746.
9.	曹春艳, 薛斌. 兔皮肤和肉膜之间放置扩张器的研究. 中华实验外科杂志, 2013, 30(2):404.
10.	França DC, de Castro AL, Soubhia AM, et al. Evaluation of the biocompatibility of silicone gel implants-histomorphometric study. Acta Inform Med, 2013, 21(2):93-97.
11.	Pasyk KA, Argenta LC, Austad ED. Histopathology of human expanded tissue. Clin Plast Surg, 1987, 14(3):435:445.
12.	陈惠平, 肖燕, 黄莹滢, 等. 早期连续快速扩张法对扩张囊外纤维包膜影响的研究. 中国美容整形外科杂志, 2013, 24(1):28-30.
13.	Tang Y, Luan J, Zhang X. Accelerating tissue expansion by application of topical papaverine cream. Plast Reconstr Surg, 2004, 114(5):1166-1169.
14.	安波, 庄洪兴, 孙广慈, 等. 扩张包膜上植皮成活的临床观察. 中华整形外科杂志, 2001, 17(2):112-113.
15.	张志宏, 王佳琦, 李文志. 包膜对猪扩张后动脉筋膜皮瓣血供影响的研究. 中国美容医学, 2011, 20(2):222-225.
16.	刘学军, 郝建忠, 喻理. 皮肤软组织扩张术中维持扩张期对扩张皮肤的影响. 国际医药卫生导报, 2002, 8(4):48-49.
17.	刘岩, 冯国平, 杨锴, 等. 面部扩张手术中纤维包膜的减张作用探讨. 中国修复重建外科杂志, 2012, 26(8):939-942.
18.	Walia I, Peer LA, Bernhard WG, et al. Does growth occur in young rabbit ear cartilage grafts transplanted in young rabbits? Plast Reconstr Surg Transplant Bull, 1962, 29(3):259-265.
19.	Gibson T, Davis W. The distortion of autogenous cartilage grafts:Its cause and prevention. Br Plast Surg, 1957-1958, 10:257-274.
20.	李太颖, 周刚, 夏双印. 自体耳软骨移植在动脉岛状皮瓣中成活规律的形态学研究. 实用美容整形外科杂志, 2003, 14(4):172-174.
21.	陈琦, 王正敏, 王德辉, 等. 皮肤扩张对自体移植软骨生长的影响. 上海医科大学学报, 2000, 27(3):226-228.
22.	Eisemann ML. The growth potential of autograft cartilage. An experimental study. Arch Otolaryngol, 1983, 109(7):469-472.

1. 庄洪兴, 蒋海越, 潘博, 等. 先天性小耳畸形的皮肤软组织扩张器法外耳再造术. 中华整形外科杂志, 2006, 22(4):286-289.
2. Kerem M, Orbay H, Sensöz O. Use of an expander temporoparietal fascial flap tecnique for total auricular reconstruction. Plast Reconstr Surg, 2007, 119(7):2320-2321.
3. Dashan Y, Haiyue J, Qinghua Y, et al. Technical innovations in ear reconstruction using a skin expander with autogenous cartilage grafts. Plast Reconstr Aesthet Surg, 2008, 61 Suppl 1:S59-69.
4. Jiang H, Pan B, Lin L, et al. Ten-year experience in microtia reconstruction using expander and autogenous cartilage. Int J Pediatr Otorhinolaryngol, 2008, 72(8):1251-1259.
5. Brent B. Technical advances in ear reconstruction with autogenous rib cartilage grafts:personal experience with 1200 cases. Plast Reconstr Surg, 1999, 104(2):319-338.
6. Kajin N, Asatoh H. Reconstruction of the auricle with autogeneous rib cartilage grafts. Adv Otorhinolaryngol, 2014, 75:61-75.
7. Sivayoham E, Woolford TJ. Current opinion on auricular reconstruction. Curr Opin Otolaryngol Head Neck Surg, 2012, 20(4):287-290.
8. Qing-Hua Y, Yu-Peng S, Haiyue J, et al. The significance of the biomechanical properties of costal cartilage in the timing of ear reconstruction surgery. Plast Reconstr Aesthet Surg, 2011, 64(6):742-746.
9. 曹春艳, 薛斌. 兔皮肤和肉膜之间放置扩张器的研究. 中华实验外科杂志, 2013, 30(2):404.
10. França DC, de Castro AL, Soubhia AM, et al. Evaluation of the biocompatibility of silicone gel implants-histomorphometric study. Acta Inform Med, 2013, 21(2):93-97.
11. Pasyk KA, Argenta LC, Austad ED. Histopathology of human expanded tissue. Clin Plast Surg, 1987, 14(3):435:445.
12. 陈惠平, 肖燕, 黄莹滢, 等. 早期连续快速扩张法对扩张囊外纤维包膜影响的研究. 中国美容整形外科杂志, 2013, 24(1):28-30.
13. Tang Y, Luan J, Zhang X. Accelerating tissue expansion by application of topical papaverine cream. Plast Reconstr Surg, 2004, 114(5):1166-1169.
14. 安波, 庄洪兴, 孙广慈, 等. 扩张包膜上植皮成活的临床观察. 中华整形外科杂志, 2001, 17(2):112-113.
15. 张志宏, 王佳琦, 李文志. 包膜对猪扩张后动脉筋膜皮瓣血供影响的研究. 中国美容医学, 2011, 20(2):222-225.
16. 刘学军, 郝建忠, 喻理. 皮肤软组织扩张术中维持扩张期对扩张皮肤的影响. 国际医药卫生导报, 2002, 8(4):48-49.
17. 刘岩, 冯国平, 杨锴, 等. 面部扩张手术中纤维包膜的减张作用探讨. 中国修复重建外科杂志, 2012, 26(8):939-942.
18. Walia I, Peer LA, Bernhard WG, et al. Does growth occur in young rabbit ear cartilage grafts transplanted in young rabbits? Plast Reconstr Surg Transplant Bull, 1962, 29(3):259-265.
19. Gibson T, Davis W. The distortion of autogenous cartilage grafts:Its cause and prevention. Br Plast Surg, 1957-1958, 10:257-274.
20. 李太颖, 周刚, 夏双印. 自体耳软骨移植在动脉岛状皮瓣中成活规律的形态学研究. 实用美容整形外科杂志, 2003, 14(4):172-174.
21. 陈琦, 王正敏, 王德辉, 等. 皮肤扩张对自体移植软骨生长的影响. 上海医科大学学报, 2000, 27(3):226-228.
22. Eisemann ML. The growth potential of autograft cartilage. An experimental study. Arch Otolaryngol, 1983, 109(7):469-472.

Chinese Journal of Reparative and Reconstructive Surgery

PRELIMINARY HISTOLOGICAL OBSERVATION OF EFFECT OF EXPANDED CAPSULE ON COSTAL CARTILAGE AUTOGRAFT

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