EXPERIMENTAL ASSESSMENT OF BLADDER REGENERATION BY COLLAGEN MEMBRANE SCAFFOLDS_Chinese Journal of Reparative and Reconstructive Surgery

Authors：

MAChao , CHENWei , JIAWeisheng , GUOYu ,  YEGang

No.1 Department of Urology, Xinqiao Hospital, Third Military Medical University, Chongqing, 400037, P. R. China;

Corresponding author：

YEGang, Email: goodocyeah@163.com

Keywords：

Bladder tissue engineering; Collagen membrane; Scaffold material; Bladder reconstruction

DOI：

10.7507/1002-1892.20140171

Video：

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Objective To observe the bladder regeneration by collagen membrane scaffolds for bladder construction to find a new alternative scaffold material. Methods Twelve healthy adult male Sprague Dawley rats, weighing 300-350 g, were randomly divided into collagen membrane scaffold group (experimental group, n=6), and sham operated group (control group, n=6). Upper hemicystectomy was performed and collagen scaffold was used for reconstruction in experimental group, while the bladder was turned over without bladder resection in control group. At 30 days after operation, the animals were sacrificed and grafts were harvested;HE staining and Masson staining were used to evaluate the bladder regeneration, immunohistochemical staining was performed with α-smooth muscleactin (α-SMA) and von Willebrand factor (vWF) markers to evaluate the percentage of α-SMA positive area and capillary number. Results The rats of 2 groups survived to the end of the experiment, and no urine leakage or infection was observed in experimental group. Histologically, control group presented a pattern of normal bladder structure, experimental group presented a pattern of almost normal urothelium with a small amount of smooth muscle cells and a thin layer of undegraded collagen fibers. Immunohistochemically, experimental group showed ingrowth of smooth muscle fibers and new capillary formation along the collagen membrane scaffolds. The percentage of α-SMA positive area and capillary number in experimental group were significantly lower than those in control group (6.49%±2.14% vs. 52.42%±1.78% and 4.83±0.75 vs. 14.83±1.17, respectively)(t=40.40, P=0.00; t=17.62, P=0.00). Conclusion The collagen membrane scaffolds could be an effective scaffold material for bladder reconstruction.

Citation： MAChao, CHENWei, JIAWeisheng, GUOYu, YEGang. EXPERIMENTAL ASSESSMENT OF BLADDER REGENERATION BY COLLAGEN MEMBRANE SCAFFOLDS. Chinese Journal of Reparative and Reconstructive Surgery, 2014, 28(6): 773-776. doi: 10.7507/1002-1892.20140171 Copy

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14.	Cen L, Liu W, Cui L, et al. Collagen tissue engineering:development of novel biomaterials and applications. Pediatr Res, 2008, 63(5):492-496.
15.	陶亮, 李强, 任昊桢, 等. 缓释bFGF胶原膜修复猪胆管缺损的实验研究. 中国修复重建外科杂志, 2013, 27(2):212-218.
16.	Li X, Sun H, Lin N, et al. Regeneration of uterine horns in rats by collagen scaffolds loaded with collagen-binding human basic fibroblast growth factor. Biomaterials, 2011, 32(32):8172-8181.
17.	Shi C, Chen W, Zhao Y, et al. Regeneration of full-thickness abdominal wall defects in rats using collagen scaffolds loaded with collagen-binding basic fibroblast growth factor. Biomaterials, 2011, 32(3):753-759.
18.	胡顺鹏, 赵洪石, 王冠聪, 等. 一种胶原支架材料的结构与性能表征. 山东大学学报:工学版, 2010, 40(4):67-71, 79.
19.	Yow SZ, Quek CH, Yim EK, et al. Collagen-based fibrous scaffold for spatial organization of encapsulated and seeded human mesenchymal stem cells. Biomaterials, 2009, 30(6):1133-1142.
20.	Laschke MW, Menger MD. Vascularization in tissue engineering:angiogenesis versus Inosculation. Eur Surg Res, 2012, 48(2):85-92.
21.	Laschke MW, Harder Y, Amon M, et al. Angiogenesis in tissue engineering:breathing life into constructed tissue substitutes. Tissue Eng, 2006, 12(8):2093-2104.
22.	Michel MC, Parra S. Similarities and differences in the autonomic control of airway and urinary bladder smooth muscle. Naunyn Schmiedebergs Arch Pharmacol, 2008, 378(2):217-224.
23.	Adamowicz J, Juszczak K, Tworkiewicz J, et al. Regeneration of smooth muscle layer is only half way to restore urinary bladder function using tissue engineering methods. European Urology Supplements, 2012, 11(1):E802-U699.

1. Atala A. Tissue engineering for the replacement of organ function in the genitourinary system. Am J Transplant, 2004, 4 Suppl 6:58-73.
2. Gurocak S, Nuininga J, Ure I, et al. Bladder augmentation:Review of the literature and recent advances. Indian J Urol, 2007, 23(4):452-457.
3. Atala A. Tissue engineering of human bladder. Br Med Bull, 2011, 97(1):81-104.
4. Di Lullo GA, Sweeney SM, Korkko J, et al. Mapping the ligand-binding sites and disease-associated mutations on the most abundant protein in the human, type I collagen. J Biol Chem, 2002, 277(6):4223-4231.
5. Parenteau-Bareil R, Gauvin R, Berthod F. Collagen-based biomaterials for tissue engineering applications. Materials, 2010, 3(3):1863-1887.
6. Montzka K, Laufer T, Becker C, et al. Microstructure and cytocompatibility of collagen matrices for urological tissue engineering. BJU Int, 2011, 107(12):1974-1981.
7. O'Brien FJ. Biomaterials & scaffolds for tissue engineering. Materials Today, 2011, 14(3):88-95.
8. Gaspar A, Moldovan L, Constantin D, et al. Collagen-based scaffolds for skin tissue engineering. J Med Life, 2011, 4(2):172-177.
9. Orlando G, Wood KJ, De Coppi P, et al. Regenerative medicine as applied to general surgery. Ann Surg, 2012, 255(5):867-880.
10. McHugh KJ, Tao SL, Saint-Geniez M. A novel porous scaffold fabrication technique for epithelial and endothelial tissue engineering. J Mater Sci Mater Med, 2013, 24(7):1659-1670.
11. Petrovic V, Stankovic J, Stefanovic V. Tissue engineering of the urinary bladder:current concepts and future perspectives. Scientific World Journal, 2011, 11:1479-1488.
12. Atala A. Regenerative medicine and tissue engineering in urology. Urol Clin North Am, 2009, 36(2):199-209.
13. Gaspar A, Moldovan L, Constantin D, et al. Collagen-based scaffolds for skin tissue engineering. J Med Life, 2011, 4(2):172-177.
14. Cen L, Liu W, Cui L, et al. Collagen tissue engineering:development of novel biomaterials and applications. Pediatr Res, 2008, 63(5):492-496.
15. 陶亮, 李强, 任昊桢, 等. 缓释bFGF胶原膜修复猪胆管缺损的实验研究. 中国修复重建外科杂志, 2013, 27(2):212-218.
16. Li X, Sun H, Lin N, et al. Regeneration of uterine horns in rats by collagen scaffolds loaded with collagen-binding human basic fibroblast growth factor. Biomaterials, 2011, 32(32):8172-8181.
17. Shi C, Chen W, Zhao Y, et al. Regeneration of full-thickness abdominal wall defects in rats using collagen scaffolds loaded with collagen-binding basic fibroblast growth factor. Biomaterials, 2011, 32(3):753-759.
18. 胡顺鹏, 赵洪石, 王冠聪, 等. 一种胶原支架材料的结构与性能表征. 山东大学学报:工学版, 2010, 40(4):67-71, 79.
19. Yow SZ, Quek CH, Yim EK, et al. Collagen-based fibrous scaffold for spatial organization of encapsulated and seeded human mesenchymal stem cells. Biomaterials, 2009, 30(6):1133-1142.
20. Laschke MW, Menger MD. Vascularization in tissue engineering:angiogenesis versus Inosculation. Eur Surg Res, 2012, 48(2):85-92.
21. Laschke MW, Harder Y, Amon M, et al. Angiogenesis in tissue engineering:breathing life into constructed tissue substitutes. Tissue Eng, 2006, 12(8):2093-2104.
22. Michel MC, Parra S. Similarities and differences in the autonomic control of airway and urinary bladder smooth muscle. Naunyn Schmiedebergs Arch Pharmacol, 2008, 378(2):217-224.
23. Adamowicz J, Juszczak K, Tworkiewicz J, et al. Regeneration of smooth muscle layer is only half way to restore urinary bladder function using tissue engineering methods. European Urology Supplements, 2012, 11(1):E802-U699.

Chinese Journal of Reparative and Reconstructive Surgery

EXPERIMENTAL ASSESSMENT OF BLADDER REGENERATION BY COLLAGEN MEMBRANE SCAFFOLDS

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